Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress.

Abstract:

BACKGROUND:Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. RESULTS:Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. CONCLUSIONS:Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Gomes DF,Batista JS,Schiavon AL,Andrade DS,Hungria M

doi

10.1186/1471-2180-12-84

subject

Has Abstract

pub_date

2012-05-30 00:00:00

pages

84

issn

1471-2180

pii

1471-2180-12-84

journal_volume

12

pub_type

杂志文章
  • Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut.

    abstract:BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1318-1

    authors: Ren F,Li X,Tang H,Jiang Q,Yun X,Fang L,Huang P,Tang Y,Li Q,Huang J,Jiao XA

    更新日期:2018-10-22 00:00:00

  • Diet, physical activity and screen time but not body mass index are associated with the gut microbiome of a diverse cohort of college students living in university housing: a cross-sectional study.

    abstract:BACKGROUND:Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between body mass index, physical activity, screen time, dietary consumption (fat, protein, carbohydrates, an...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1362-x

    authors: Whisner CM,Maldonado J,Dente B,Krajmalnik-Brown R,Bruening M

    更新日期:2018-12-12 00:00:00

  • atpE gene as a new useful specific molecular target to quantify Mycobacterium in environmental samples.

    abstract:BACKGROUND:The environment is the likely source of many pathogenic mycobacterial species but detection of mycobacteria by bacteriological tools is generally difficult and time-consuming. Consequently, several molecular targets based on the sequences of housekeeping genes, non-functional RNA and structural ribosomal RNA...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-277

    authors: Radomski N,Roguet A,Lucas FS,Veyrier FJ,Cambau E,Accrombessi H,Moilleron R,Behr MA,Moulin L

    更新日期:2013-12-03 00:00:00

  • A ferritin-like protein with antioxidant activity in Ureaplasma urealyticum.

    abstract:BACKGROUND:Ureaplasma urealyticum is a major pathogen associated with many diseases. The ability of U. urealyticum to protect itself from oxidative stress is likely to be important for its pathogenesis and survival, but its oxidative stress tolerance mechanisms remain unclear. This study investigates the antioxidant ac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0485-6

    authors: Dai G,Li R,Chen H,Jiang C,You X,Wu Y

    更新日期:2015-07-26 00:00:00

  • Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris.

    abstract:BACKGROUND:Extracellular aspartic proteinase (MCAP) produced by Mucor circinelloides in solid state fermentations has been shown to possess milk clotting activity and represents a potential replacement for bovine chymosin in cheese manufacturing. Despite its prospects in the dairy industry, the molecular characteristic...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-250

    authors: Gama Salgado JA,Kangwa M,Fernandez-Lahore M

    更新日期:2013-11-09 00:00:00

  • Pyrosequencing analysis revealed complex endogenetic microorganism community from natural DongChong XiaCao and its microhabitat.

    abstract:BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dep...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0813-5

    authors: Xia F,Liu Y,Guo MY,Shen GR,Lin J,Zhou XW

    更新日期:2016-08-26 00:00:00

  • Transcriptomic and proteomic analyses of core metabolism in Clostridium termitidis CT1112 during growth on α-cellulose, xylan, cellobiose and xylose.

    abstract:BACKGROUND:Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae. It has the ability to hydrolyze both cellulose and hemicellulose, and ferment the degradation products to acetate, fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0711-x

    authors: Munir RI,Spicer V,Krokhin OV,Shamshurin D,Zhang X,Taillefer M,Blunt W,Cicek N,Sparling R,Levin DB

    更新日期:2016-05-23 00:00:00

  • Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens.

    abstract:BACKGROUND:The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS:In t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1440-8

    authors: Hazarika DJ,Goswami G,Gautom T,Parveen A,Das P,Barooah M,Boro RC

    更新日期:2019-04-02 00:00:00

  • Variability of antibiotic susceptibility and toxin production of Staphylococcus aureus strains isolated from skin, soft tissue, and bone related infections.

    abstract:BACKGROUND:Staphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-188

    authors: Sina H,Ahoyo TA,Moussaoui W,Keller D,Bankolé HS,Barogui Y,Stienstra Y,Kotchoni SO,Prévost G,Baba-Moussa L

    更新日期:2013-08-08 00:00:00

  • In silico evolutionary analysis of Helicobacter pylori outer membrane phospholipase A (OMPLA).

    abstract:BACKGROUND:In the past decade, researchers have proposed that the pldA gene for outer membrane phospholipase A (OMPLA) is important for bacterial colonization of the human gastric ventricle. Several conserved Helicobacter pylori genes have distinct genotypes in different parts of the world, biogeographic patterns that ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-206

    authors: Vollan HS,Tannaes T,Yamaoka Y,Bukholm G

    更新日期:2012-09-13 00:00:00

  • Wolbachia infection in Argentinean populations of Anastrepha fraterculus sp1: preliminary evidence of sex ratio distortion by one of two strains.

    abstract:BACKGROUND:Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic in...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1652-y

    authors: Conte CA,Segura DF,Milla FH,Augustinos A,Cladera JL,Bourtzis K,Lanzavecchia SB

    更新日期:2019-12-24 00:00:00

  • Modelling invasive group A streptococcal disease using bioluminescence.

    abstract:BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1200-1

    authors: Lamb LE,Zhi X,Alam F,Pyzio M,Scudamore CL,Wiles S,Sriskandan S

    更新日期:2018-06-19 00:00:00

  • Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanases and polygalacturonases of Fusarium virguliforme.

    abstract:BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0761-0

    authors: Chang HX,Yendrek CR,Caetano-Anolles G,Hartman GL

    更新日期:2016-07-12 00:00:00

  • The process-related dynamics of microbial community during a simulated fermentation of Chinese strong-flavored liquor.

    abstract:BACKGROUND:Famous Chinese strong-flavored liquor (CSFL) is brewed by microbial consortia in a special fermentation pit (FT). However, the fermentation process was not fully understood owing to the complicate community structure and metabolism. In this study, the process-related dynamics of microbial communities and mai...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1106-3

    authors: Zhang Y,Zhu X,Li X,Tao Y,Jia J,He X

    更新日期:2017-09-15 00:00:00

  • The prevalence of gene duplications and their ancient origin in Rhodobacter sphaeroides 2.4.1.

    abstract:BACKGROUND:Rhodobacter sphaeroides 2.4.1 is a metabolically versatile organism that belongs to α-3 subdivision of Proteobacteria. The present study was to identify the extent, history, and role of gene duplications in R. sphaeroides 2.4.1, an organism that possesses two chromosomes. RESULTS:A protein similarity search...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-331

    authors: Bavishi A,Lin L,Schroeder K,Peters A,Cho H,Choudhary M

    更新日期:2010-12-30 00:00:00

  • The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis.

    abstract:BACKGROUND:A growing body of evidence suggests that Mycobacterium tuberculosis (Mtb) uses the host's cholesterol as a source of carbon and energy during infection. Strains defective in cholesterol transport or degradation exhibit attenuated growth in activated macrophages and diminished infectivity in animal models. Th...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-43

    authors: Brzezinska M,Szulc I,Brzostek A,Klink M,Kielbik M,Sulowska Z,Pawelczyk J,Dziadek J

    更新日期:2013-02-20 00:00:00

  • Differences between predicted outer membrane proteins of genotype 1 and 2 Mannheimia haemolytica.

    abstract:BACKGROUND:Mannheimia haemolytica strains isolated from North American cattle have been classified into two genotypes (1 and 2). Although members of both genotypes have been isolated from the upper and lower respiratory tracts of cattle with or without bovine respiratory disease (BRD), genotype 2 strains are much more ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01932-2

    authors: Clawson ML,Schuller G,Dickey AM,Bono JL,Murray RW,Sweeney MT,Apley MD,DeDonder KD,Capik SF,Larson RL,Lubbers BV,White BJ,Blom J,Chitko-McKown CG,Brichta-Harhay DM,Smith TPL

    更新日期:2020-08-12 00:00:00

  • Bifidobacteria strains isolated from stools of iron deficient infants can efficiently sequester iron.

    abstract:BACKGROUND:Bifidobacteria is one of the major gut commensal groups found in infants. Their colonization is commonly associated with beneficial effects to the host through mechanisms like niche occupation and nutrient competition against pathogenic bacteria. Iron is an essential element necessary for most microorganisms...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0334-z

    authors: Vazquez-Gutierrez P,Lacroix C,Jaeggi T,Zeder C,Zimmerman MB,Chassard C

    更新日期:2015-01-16 00:00:00

  • Inhibition of HSP70 reduces porcine reproductive and respiratory syndrome virus replication in vitro.

    abstract:BACKGROUND:Successful viral infection requires the involvement of host cellular factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by numerous viruses to promote the folding, maturation, or assembly of viral proteins. We have previously shown that HSP70 is significantly elevated in porcine repr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-64

    authors: Gao J,Xiao S,Liu X,Wang L,Ji Q,Mo D,Chen Y

    更新日期:2014-03-13 00:00:00

  • Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs.

    abstract:BACKGROUND:Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxyg...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-55

    authors: Bo Andersen J,Roldgaard BB,Christensen BB,Licht TR

    更新日期:2007-06-14 00:00:00

  • Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.

    abstract:BACKGROUND:Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1423-9

    authors: Sánchez MC,Romero-Lastra P,Ribeiro-Vidal H,Llama-Palacios A,Figuero E,Herrera D,Sanz M

    更新日期:2019-03-12 00:00:00

  • Characterization of the interaction of staphylococcal enterotoxin B with CD1d expressed in human renal proximal tubule epithelial cells.

    abstract:BACKGROUND:Participation of renal cells in the pathogenesis of staphylococcal enterotoxin B (SEB) is critical for late cleansing and sequestration of the antigens facilitated by CD1d mediated antigen sensing and recognition. This is a noted deviation from the typical antigen recognition process that recruits the major ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0344-5

    authors: Hammamieh R,Chakraborty N,Lin Y,Shupp JW,Miller SA,Morris S,Jett M

    更新日期:2015-02-04 00:00:00

  • Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression.

    abstract:BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-167

    authors: Weeks JN,Galindo CL,Drake KL,Adams GL,Garner HR,Ficht TA

    更新日期:2010-06-08 00:00:00

  • Cloning and disruption of the UeArginase in Ustilago esculenta: evidence for a role of arginine in its dimorphic transition.

    abstract:BACKGROUND:Ustilago esculenta, a typical dimorphic fungus could infect Zizania latifolia and induce host stem swollen to form an edible vegetable called Jiaobai in China. The strains differentiation especially in the mating ability and pathogenicity is closely related to different phenotypes of Jiaobai formed in the fi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1588-2

    authors: Zhang Y,Wu M,Ge Q,Yang M,Xia W,Cui H,Yu X,Zhang S,Ye Z

    更新日期:2019-09-05 00:00:00

  • Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides.

    abstract:BACKGROUND:Rhodosporidium toruloides is a β-carotenoid accumulating, oleaginous yeast that has great biotechnological potential. The lack of reliable and efficient genetic manipulation tools have been a major hurdle blocking its adoption as a biotechnology platform. RESULTS:We report for the first time the development...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-50

    authors: Koh CM,Liu Y,Moehninsi,Du M,Ji L

    更新日期:2014-02-27 00:00:00

  • Acquisition of the lac operon by Salmonella enterica.

    abstract:BACKGROUND:Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0511-8

    authors: Leonard SR,Lacher DW,Lampel KA

    更新日期:2015-08-25 00:00:00

  • Development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry.

    abstract:BACKGROUND:Bloodstream infections represent serious conditions carrying a high mortality and morbidity rate. Rapid identification of microorganisms and prompt institution of adequate antimicrobial therapy is of utmost importance for a successful outcome. Aiming at the development of a rapid, simplified and efficient pr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0594-2

    authors: Jakovljev A,Bergh K

    更新日期:2015-11-06 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-69

    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Identification of Enterobacter sakazakii from closely related species: the use of artificial neural networks in the analysis of biochemical and 16S rDNA data.

    abstract:BACKGROUND:Enterobacter sakazakii is an emergent pathogen associated with ingestion of infant formula and accurate identification is important in both industrial and clinical settings. Bacterial species can be difficult to accurately characterise from complex biochemical datasets and computer algorithms can potentially...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-28

    authors: Iversen C,Lancashire L,Waddington M,Forsythe S,Ball G

    更新日期:2006-03-13 00:00:00

  • Effectiveness of the standard and an alternative set of Streptococcus pneumoniae multi locus sequence typing primers.

    abstract:BACKGROUND:Multi-locus sequence typing (MLST) is a portable, broadly applicable method for classifying bacterial isolates at an intra-species level. This methodology provides clinical and scientific investigators with a standardized means of monitoring evolution within bacterial populations. MLST uses the DNA sequences...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-143

    authors: Adamiak P,Vanderkooi OG,Kellner JD,Schryvers AB,Bettinger JA,Alcantara J

    更新日期:2014-06-03 00:00:00