Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris.

abstract：BACKGROUND:DING proteins constitute a conserved and broadly distributed set of proteins found in bacteria, fungi, plants and animals (including humans). Characterization of DING proteins from animal and plant tissues indicated ligand-binding ability suggesting a role for DING proteins in cell signaling and biomineraliz...

journal_title：BMC microbiology

authors： Zhang XX,Scott K,Meffin R,Rainey PB

更新日期：2007-12-18 00:00:00

abstract：BACKGROUND:Bacteria release a wide variety of small molecules including cell-to-cell signaling compounds. Gram-negative bacteria use a variety of self-produced autoinducers such as acylated homoserine lactones (acyl-HSLs) as signal compounds for quorum sensing (QS) within and between bacterial species. QS plays a signi...

journal_title：BMC microbiology

authors： Minagawa S,Inami H,Kato T,Sawada S,Yasuki T,Miyairi S,Horikawa M,Okuda J,Gotoh N

更新日期：2012-05-10 00:00:00

abstract：BACKGROUND:Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS:Here we provide characterization of the first genome sequence for this species, strai...

journal_title：BMC microbiology

authors： Richards VP,Zadoks RN,Pavinski Bitar PD,Lefébure T,Lang P,Werner B,Tikofsky L,Moroni P,Stanhope MJ

更新日期：2012-12-18 00:00:00

abstract：BACKGROUND:Staphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic ...

journal_title：BMC microbiology

authors： Sina H,Ahoyo TA,Moussaoui W,Keller D,Bankolé HS,Barogui Y,Stienstra Y,Kotchoni SO,Prévost G,Baba-Moussa L

更新日期：2013-08-08 00:00:00

abstract：BACKGROUND:Many bacterial extracytoplasmic proteins are stabilized by intramolecular disulfide bridges that are formed post-translationally between their cysteine residues. This protein modification plays an important role in bacterial pathogenesis, and is facilitated by the Dsb (disulfide bond) family of the redox pro...

journal_title：BMC microbiology

authors： Grabowska AD,Wandel MP,Łasica AM,Nesteruk M,Roszczenko P,Wyszyńska A,Godlewska R,Jagusztyn-Krynicka EK

更新日期：2011-07-25 00:00:00

abstract：BACKGROUND:Legionella testing conducted at environmental laboratories plays an essential role in assessing the risk of disease transmission associated with water systems. However, drawbacks of culture-based methodology used for Legionella enumeration can have great impact on the results and interpretation which togethe...

journal_title：BMC microbiology

authors： Díaz-Flores Á,Montero JC,Castro FJ,Alejandres EM,Bayón C,Solís I,Fernández-Lafuente R,Rodríguez G

更新日期：2015-04-29 00:00:00

abstract：BACKGROUND:The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have examined the impact of laboratory contamination on samples collected from the lower airways by pro...

journal_title：BMC microbiology

authors： Drengenes C,Wiker HG,Kalananthan T,Nordeide E,Eagan TML,Nielsen R

更新日期：2019-08-14 00:00:00

abstract：BACKGROUND:The Beijing lineage of Mycobacterium tuberculosis is causing concern due to its global distribution and its involvement in severe outbreaks. Studies focused on this lineage are mainly restricted to geographical settings where its prevalence is high, whereas those in other areas are scarce. In this study, we ...

journal_title：BMC microbiology

authors： Alonso M,Alonso Rodriguez N,Garzelli C,Martínez Lirola M,Herranz M,Samper S,Ruiz Serrano MJ,Bouza E,García de Viedma D

更新日期：2010-05-25 00:00:00

abstract：BACKGROUND:Chlortetracycline (CTC) is one of the commercially important tetracyclines (TCs) family product and is mainly produced by Streptomyces. CTC is still in a great demand due to its broad-spectrum activity against pathogens. Engineering transcriptional control allows the cell to allocate its valuable resources t...

journal_title：BMC microbiology

authors： Kong L,Liu J,Zheng X,Deng Z,You D

更新日期：2019-12-10 00:00:00

abstract：BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG...

journal_title：BMC microbiology

authors： Tazumi A,Kakinuma Y,Misawa N,Moore JE,Millar BC,Matsuda M

更新日期：2009-12-11 00:00:00

abstract：BACKGROUND:Typically, raw meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of raw meat, ...

journal_title：BMC microbiology

authors： Dsani E,Afari EA,Danso-Appiah A,Kenu E,Kaburi BB,Egyir B

更新日期：2020-08-14 00:00:00

abstract：BACKGROUND:Bacterial and cellular genotyping is becoming increasingly important in the diagnosis of infectious diseases. However, difficulties in obtaining sufficient amount of bacterial and cellular DNA extracted from the same human biopsy specimens is often a limiting factor. In this study, total DNA (host and bacter...

journal_title：BMC microbiology

authors： Ryberg A,Borch K,Sun YQ,Monstein HJ

更新日期：2008-10-08 00:00:00

abstract：BACKGROUND:H-NS is a DNA-binding protein with central roles in gene regulation and nucleoid structuring in Escherichia coli. There are over 60 genes that are influenced by H-NS many of which are involved in metabolism. To determine the significance of H-NS-regulated genes in metabolism and stress tolerance, an hns muta...

journal_title：BMC microbiology

authors： Erol I,Jeong KC,Baumler DJ,Vykhodets B,Choi SH,Kaspar CW

更新日期：2006-08-15 00:00:00

abstract：BACKGROUND:The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to Abiotrophia/Granulicatella. R...

journal_title：BMC microbiology

authors： Quevedo B,Giertsen E,Zijnge V,Lüthi-Schaller H,Guggenheim B,Thurnheer T,Gmür R

更新日期：2011-01-19 00:00:00

abstract：BACKGROUND:Ureaplasma urealyticum is a major pathogen associated with many diseases. The ability of U. urealyticum to protect itself from oxidative stress is likely to be important for its pathogenesis and survival, but its oxidative stress tolerance mechanisms remain unclear. This study investigates the antioxidant ac...

journal_title：BMC microbiology

authors： Dai G,Li R,Chen H,Jiang C,You X,Wu Y

更新日期：2015-07-26 00:00:00

abstract：BACKGROUND:The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qP...

journal_title：BMC microbiology

authors： Le Gall F,Le Berre R,Rosec S,Hardy J,Gouriou S,Boisramé-Gastrin S,Vallet S,Rault G,Payan C,Héry-Arnaud G

更新日期：2013-06-21 00:00:00

abstract：BACKGROUND:The Uzon Caldera is one of the places on our planet with unique geological, ecological, and microbiological characteristics. Uzon oil is the youngest on Earth. Uzon oil has unique composition, with low proportion of heavy fractions and relatively high content of saturated hydrocarbons. Microbial communities ...

journal_title：BMC microbiology

authors： Peltek SE,Bryanskaya AV,Uvarova YE,Rozanov AS,Ivanisenko TV,Ivanisenko VA,Lazareva EV,Saik OV,Efimov VM,Zhmodik SM,Taran OP,Slynko NM,Shekhovtsov SV,Parmon VN,Dobretsov NL,Kolchanov NA

更新日期：2020-11-24 00:00:00

abstract：BACKGROUND:Sinorhizobium meliloti is an agriculturally important model symbiont. There is an ongoing need to update and improve its genome annotation. In this study, we used a high-throughput pyrosequencing approach to sequence the transcriptome of S. meliloti, and search for new bacterial genes missed in the previous ...

journal_title：BMC microbiology

authors： Mao C,Evans C,Jensen RV,Sobral BW

更新日期：2008-05-02 00:00:00

abstract：BACKGROUND:Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between body mass index, physical activity, screen time, dietary consumption (fat, protein, carbohydrates, an...

journal_title：BMC microbiology

authors： Whisner CM,Maldonado J,Dente B,Krajmalnik-Brown R,Bruening M

更新日期：2018-12-12 00:00:00

abstract：BACKGROUND:Antimicrobial helper-compounds may reverse antimicrobial resistance. Sertraline, a antidepressant drug, has been suggested as a tetracycline helper-compound. Tetracycline is the preferred antimicrobial for treatment of enteric diseases in pigs. This study is the first to evaluate the potency of sertraline as...

journal_title：BMC microbiology

authors： Kromann S,Hvidtfeldt A,Boye M,Sørensen DB,Jørgensen S,Nielsen JP,Olsen RH

更新日期：2019-01-11 00:00:00

abstract：BACKGROUND:The heme acquisition machinery in Streptococcus pyogenes is believed to consist of the surface proteins, Shr and Shp, and heme-specific ATP-binding cassette transporter HtsABC. Shp has been shown to rapidly transfer its heme to the lipoprotein component, HtsA, of HtsABC. The function of Shr and the heme sour...

journal_title：BMC microbiology

authors： Zhu H,Liu M,Lei B

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:Bacteria adapted to live within animals can protect their hosts against harmful infections. Beyond antagonism with pathogens, a 'defensive' bacterial symbiont could engage in additional interactions with other colonizing micro-organisms. A single bacterium might thus have cascading ecological impacts on the ...

journal_title：BMC microbiology

authors： Dahan D,Preston GM,Sealey J,King KC

更新日期：2020-06-15 00:00:00

abstract：BACKGROUND:Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. RESULTS:In this study, we have establishe...

journal_title：BMC microbiology

authors： Busch CJ,Vogt A,Mochizuki K

更新日期：2010-07-13 00:00:00

abstract：BACKGROUND:Biofilm is a fundamental bacterial survival mode which proceeds through three main generalized phases: adhesion, maturation, and dispersion. Lactobacilli spp. (LB) are critical components of gut and reproductive health and are widely used probiotics. Evaluation of time-dependent mechanisms of biofilm formati...

journal_title：BMC microbiology

authors： Martinez S,Garcia JG,Williams R,Elmassry M,West A,Hamood A,Hurtado D,Gudenkauf B,Ventolini G,Schlabritz-Loutsevitch N

更新日期：2020-03-24 00:00:00

abstract：BACKGROUND:Coxiella burnetii is a Gram-negative intracellular bacterial pathogen that replicates within a phagolysosome-like parasitophorous vacuole (PV) of macrophages. PV formation requires delivery of effector proteins directly into the host cell cytoplasm by a type IVB secretion system. However, additional secretio...

journal_title：BMC microbiology

authors： Stead CM,Omsland A,Beare PA,Sandoz KM,Heinzen RA

更新日期：2013-10-05 00:00:00

abstract：BACKGROUND:Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objecti...

journal_title：BMC microbiology

authors： Rodriguez C,Taminiau B,Korsak N,Avesani V,Van Broeck J,Brach P,Delmée M,Daube G

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. Our knowledge of PAH-degrading bacterial populations in the marine environment is still very limited, and mainly originat...

journal_title：BMC microbiology

authors： Lozada M,Riva Mercadal JP,Guerrero LD,Di Marzio WD,Ferrero MA,Dionisi HM

更新日期：2008-03-25 00:00:00

abstract：BACKGROUND:Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In ...

journal_title：BMC microbiology

authors： Maquart M,Le Flèche P,Foster G,Tryland M,Ramisse F,Djønne B,Al Dahouk S,Jacques I,Neubauer H,Walravens K,Godfroid J,Cloeckaert A,Vergnaud G

更新日期：2009-07-20 00:00:00

abstract：BACKGROUND:It is known that insects and crustaceans can carry simultaneous, active infections of two or more viruses without showing signs of disease, but it was not clear whether co-infecting viruses occupied the same cells or different cells in common target tissues. Our previous work showed that successive challenge...

journal_title：BMC microbiology

authors： Kanthong N,Khemnu N,Pattanakitsakul SN,Malasit P,Flegel TW

更新日期：2010-01-20 00:00:00

abstract：BACKGROUND:Most living cells uptake phosphate, an indispensable nutrient for growth from their natural environment. In Synechocystis sp. PCC 6803, the cells lack phosphate-inorganic transport (Pit) system but contain two phosphate-specific transport (Pst) systems, Pst1 and Pst2. We investigated the kinetics of Pi uptak...

journal_title：BMC microbiology

authors： Burut-Archanai S,Eaton-Rye JJ,Incharoensakdi A

更新日期：2011-10-11 00:00:00