Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms.


BACKGROUND:The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to Abiotrophia/Granulicatella. RESULTS:As lactobacilli are known for notorious resistance to probe penetration, probe-specific assay protocols were experimentally developed to provide maximum cell wall permeability, probe accessibility, hybridization stringency, and fluorescence intensity. The new assays were then applied in a pilot study to three biofilm samples harvested from variably demineralized bovine enamel discs that had been carried in situ for 10 days by different volunteers. Best probe penetration and fluorescent labeling of reference strains were obtained after combined lysozyme and achromopeptidase treatment followed by exposure to lipase. Hybridization stringency had to be established strictly for each probe. Thereafter all probes showed the expected specificity with reference strains and labeled the anticipated morphotypes in dental plaques. Applied to in situ grown biofilms the set of probes detected only Lactobacillus fermentum and bacteria of the Lactobacillus casei group. The most cariogenic biofilm contained two orders of magnitude higher L. fermentum cell numbers than the other biofilms. Abiotrophia/Granulicatella and streptococci from the mitis group were found in all samples at high levels, whereas Streptococcus mutans was detected in only one sample in very low numbers. CONCLUSIONS:Application of these new group- and species-specific FISH probes to oral biofilm-forming lactic acid bacteria will allow a clearer understanding of the supragingival biome, its spatial architecture and of structure-function relationships implicated during plaque homeostasis and caries development. The probes should prove of value far beyond the field of oral microbiology, as many of them detect non-oral species and phylogenetic groups of importance in a variety of medical conditions and the food industry.


BMC Microbiol


BMC microbiology


Quevedo B,Giertsen E,Zijnge V,Lüthi-Schaller H,Guggenheim B,Thurnheer T,Gmür R




Has Abstract


2011-01-19 00:00:00










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    pub_type: 杂志文章


    authors: Yindeeyoungyeon W,Likitvivatanavong S,Palittapongarnpim P

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  • Early development of Moniliophthora perniciosa basidiomata and developmentally regulated genes.

    abstract:BACKGROUND:The hemibiotrophic fungus Moniliophthora perniciosa is the causal agent of Witches' broom, a disease of Theobroma cacao. The pathogen life cycle ends with the production of basidiocarps in dead tissues of the infected host. This structure generates millions of basidiospores that reinfect young tissues of the...

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    pub_type: 杂志文章


    authors: Pires AB,Gramacho KP,Silva DC,Góes-Neto A,Silva MM,Muniz-Sobrinho JS,Porto RF,Villela-Dias C,Brendel M,Cascardo JC,Pereira GA

    更新日期:2009-08-04 00:00:00

  • Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection.

    abstract:BACKGROUND:A disrupted cell cycle progression of hepatocytes was reported in chronic hepatitis C virus (HCV) infection, which can contribute significantly in the associated pathogenesis. The present study aimed to further elaborate these disruptions by evaluating the expression of key cell cycle and apoptotic proteins ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Sarfraz S,Hamid S,Siddiqui A,Hussain S,Pervez S,Alexander G

    更新日期:2008-08-05 00:00:00

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    pub_type: 杂志文章


    authors: Mempin R,Tran H,Chen C,Gong H,Kim Ho K,Lu S

    更新日期:2013-12-24 00:00:00

  • Biphasic toxicodynamic features of some antimicrobial agents on microbial growth: a dynamic mathematical model and its implications on hormesis.

    abstract:BACKGROUND:In the present work, we describe a group of anomalous dose-response (DR) profiles and develop a dynamic model that is able to explain them. Responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (Carnobacterium piscicola and Leuc...

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    pub_type: 杂志文章


    authors: Murado MA,Vázquez JA

    更新日期:2010-08-19 00:00:00

  • In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice.

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    pub_type: 杂志文章


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    更新日期:2018-10-30 00:00:00

  • Adaptive response to iterative passages of five Lactobacillus species in simulated vaginal fluid.

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    pub_type: 杂志文章


    authors: Brandt K,Barrangou R

    更新日期:2020-11-10 00:00:00

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    pub_type: 杂志文章


    authors: Ćudić E,Surmann K,Panasia G,Hammer E,Hunke S

    更新日期:2017-09-18 00:00:00

  • Colony shape as a genetic trait in the pattern-forming Bacillus mycoides.

    abstract:BACKGROUND:Bacillus mycoides Flügge, a Gram-positive, non-motile soil bacterium assigned to Bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (SIN or DX morphotypes). The molecular mechanism causing asymmetric curving is not known: ou...

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    pub_type: 杂志文章


    authors: Di Franco C,Beccari E,Santini T,Pisaneschi G,Tecce G

    更新日期:2002-11-13 00:00:00

  • Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection.

    abstract:BACKGROUND:Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with em...

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    pub_type: 杂志文章


    authors: França TG,Ishikawa LL,Zorzella-Pezavento SF,Chiuso-Minicucci F,Guerino CP,da Cunha Mde L,Sartori A

    更新日期:2009-11-23 00:00:00

  • 16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform.

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    pub_type: 杂志文章


    authors: Fouhy F,Clooney AG,Stanton C,Claesson MJ,Cotter PD

    更新日期:2016-06-24 00:00:00

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    pub_type: 杂志文章


    authors: Zhang Y,Lu Z,Liu S,Yang Y,He Z,Ren Z,Zhou J,Li D

    更新日期:2013-03-29 00:00:00

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    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

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    pub_type: 杂志文章


    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

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    pub_type: 杂志文章


    authors: Nováková E,Hypsa V,Moran NA

    更新日期:2009-07-20 00:00:00

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    authors: Feye KM,Swaggerty CL,Kogut MH,Ricke SC,Piva A,Grilli E

    更新日期:2020-11-02 00:00:00

  • A high throughput multiplex PCR assay for simultaneous detection of seven aminoglycoside-resistance genes in Enterobacteriaceae.

    abstract:BACKGROUND:The aminoglycoside-resistance genes encoding aminoglycoside modifying enzymes and 16S rRNA methyltransferases are main factors contributing to increasing resistance to aminoglycosides. Characterization and distribution of antimicrobial resistance gene profiles provide important information on the potential d...

    journal_title:BMC microbiology

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    authors: Hu X,Xu B,Yang Y,Liu D,Yang M,Wang J,Shen H,Zhou X,Ma X

    更新日期:2013-03-14 00:00:00

  • Laboratory divergence of Methylobacterium extorquens AM1 through unintended domestication and past selection for antibiotic resistance.

    abstract:BACKGROUND:A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discove...

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    authors: Carroll SM,Xue KS,Marx CJ

    更新日期:2014-01-02 00:00:00

  • Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens.

    abstract:BACKGROUND:The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS:In t...

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    authors: Hazarika DJ,Goswami G,Gautom T,Parveen A,Das P,Barooah M,Boro RC

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  • Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.

    abstract:BACKGROUND:The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Bai Y,Wang R,Song W,Li L,Santoferrara LF,Hu X

    更新日期:2020-12-14 00:00:00

  • Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination.

    abstract:BACKGROUND:Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic...

    journal_title:BMC microbiology

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    authors: Carlos C,Pires MM,Stoppe NC,Hachich EM,Sato MI,Gomes TA,Amaral LA,Ottoboni LM

    更新日期:2010-06-01 00:00:00

  • Mechanistic studies of intracellular delivery of proteins by cell-penetrating peptides in cyanobacteria.

    abstract:BACKGROUND:The plasma membrane plays an essential role in selective permeability, compartmentalization, osmotic balance, and cellular uptake. The characteristics and functions of cyanobacterial membranes have been extensively investigated in recent years. Cell-penetrating peptides (CPPs) are special nanocarriers that c...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Liu BR,Huang YW,Lee HJ

    更新日期:2013-03-14 00:00:00

  • Phenotypic and genotypic characterisation of multiple antibiotic-resistant Staphylococcus aureus exposed to subinhibitory levels of oxacillin and levofloxacin.

    abstract:BACKGROUND:The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern i...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Jo A,Ahn J

    更新日期:2016-07-29 00:00:00

  • Influence of internalin A murinisation on host resistance to orally acquired listeriosis in mice.

    abstract:BACKGROUND:The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Bergmann S,Beard PM,Pasche B,Lienenklaus S,Weiss S,Gahan CG,Schughart K,Lengeling A

    更新日期:2013-04-23 00:00:00

  • High-quality genome sequence assembly of R.A73 Enterococcus faecium isolated from freshwater fish mucus.

    abstract:BACKGROUND:Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that pr...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: El Jeni R,Ghedira K,El Bour M,Abdelhak S,Benkahla A,Bouhaouala-Zahar B

    更新日期:2020-10-23 00:00:00

  • Analysis of and function predictions for previously conserved hypothetical or putative proteins in Blochmannia floridanus.

    abstract:BACKGROUND:There is an increasing interest to better understand endosymbiont capabilities in insects both from an ecological point of view and for pest control. Blochmannia floridanus provides important nutrients for its host, the ant Camponotus, while the bacterium in return is provided with a niche to proliferate. Bl...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Gaudermann P,Vogl I,Zientz E,Silva FJ,Moya A,Gross R,Dandekar T

    更新日期:2006-01-09 00:00:00

  • Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast.

    abstract:BACKGROUND:Meiotic cells undergo two rounds of nuclear division and generate gametes. Previous studies have indicated that a number of transcription factors modulate the transcriptome in successive waves during meiosis and spore formation in fission yeast. However, the mechanisms underlying the post-transcriptional reg...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Togashi N,Yamashita A,Sato M,Yamamoto M

    更新日期:2014-07-15 00:00:00

  • Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

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    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Sachse K,Laroucau K,Hotzel H,Schubert E,Ehricht R,Slickers P

    更新日期:2008-04-17 00:00:00

  • A Candida albicans early stage biofilm detachment event in rich medium.

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    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Sellam A,Al-Niemi T,McInnerney K,Brumfield S,Nantel A,Suci PA

    更新日期:2009-02-02 00:00:00

  • Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti.

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    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Zhang Y,Smallbone LA,diCenzo GC,Morton R,Finan TM

    更新日期:2016-07-26 00:00:00

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    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Clawson ML,Schuller G,Dickey AM,Bono JL,Murray RW,Sweeney MT,Apley MD,DeDonder KD,Capik SF,Larson RL,Lubbers BV,White BJ,Blom J,Chitko-McKown CG,Brichta-Harhay DM,Smith TPL

    更新日期:2020-08-12 00:00:00