Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms.

Abstract:

BACKGROUND:The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to Abiotrophia/Granulicatella. RESULTS:As lactobacilli are known for notorious resistance to probe penetration, probe-specific assay protocols were experimentally developed to provide maximum cell wall permeability, probe accessibility, hybridization stringency, and fluorescence intensity. The new assays were then applied in a pilot study to three biofilm samples harvested from variably demineralized bovine enamel discs that had been carried in situ for 10 days by different volunteers. Best probe penetration and fluorescent labeling of reference strains were obtained after combined lysozyme and achromopeptidase treatment followed by exposure to lipase. Hybridization stringency had to be established strictly for each probe. Thereafter all probes showed the expected specificity with reference strains and labeled the anticipated morphotypes in dental plaques. Applied to in situ grown biofilms the set of probes detected only Lactobacillus fermentum and bacteria of the Lactobacillus casei group. The most cariogenic biofilm contained two orders of magnitude higher L. fermentum cell numbers than the other biofilms. Abiotrophia/Granulicatella and streptococci from the mitis group were found in all samples at high levels, whereas Streptococcus mutans was detected in only one sample in very low numbers. CONCLUSIONS:Application of these new group- and species-specific FISH probes to oral biofilm-forming lactic acid bacteria will allow a clearer understanding of the supragingival biome, its spatial architecture and of structure-function relationships implicated during plaque homeostasis and caries development. The probes should prove of value far beyond the field of oral microbiology, as many of them detect non-oral species and phylogenetic groups of importance in a variety of medical conditions and the food industry.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Quevedo B,Giertsen E,Zijnge V,Lüthi-Schaller H,Guggenheim B,Thurnheer T,Gmür R

doi

10.1186/1471-2180-11-14

subject

Has Abstract

pub_date

2011-01-19 00:00:00

pages

14

issn

1471-2180

pii

1471-2180-11-14

journal_volume

11

pub_type

杂志文章
  • Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis.

    abstract:BACKGROUND:Alpha-isopropylmalate synthase (alpha-IPMS) is the key enzyme that catalyzes the first committed step in the leucine biosynthetic pathway. The gene encoding alpha-IPMS in Mycobacterium tuberculosis, leuA, is polymorphic due to the insertion of 57-bp repeat units referred to as Variable Number of Tandem Repea...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-122

    authors: Yindeeyoungyeon W,Likitvivatanavong S,Palittapongarnpim P

    更新日期:2009-06-09 00:00:00

  • Early development of Moniliophthora perniciosa basidiomata and developmentally regulated genes.

    abstract:BACKGROUND:The hemibiotrophic fungus Moniliophthora perniciosa is the causal agent of Witches' broom, a disease of Theobroma cacao. The pathogen life cycle ends with the production of basidiocarps in dead tissues of the infected host. This structure generates millions of basidiospores that reinfect young tissues of the...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-158

    authors: Pires AB,Gramacho KP,Silva DC,Góes-Neto A,Silva MM,Muniz-Sobrinho JS,Porto RF,Villela-Dias C,Brendel M,Cascardo JC,Pereira GA

    更新日期:2009-08-04 00:00:00

  • Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection.

    abstract:BACKGROUND:A disrupted cell cycle progression of hepatocytes was reported in chronic hepatitis C virus (HCV) infection, which can contribute significantly in the associated pathogenesis. The present study aimed to further elaborate these disruptions by evaluating the expression of key cell cycle and apoptotic proteins ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-133

    authors: Sarfraz S,Hamid S,Siddiqui A,Hussain S,Pervez S,Alexander G

    更新日期:2008-08-05 00:00:00

  • Release of extracellular ATP by bacteria during growth.

    abstract:BACKGROUND:Adenosine triphosphate (ATP) is used as an intracellular energy source by all living organisms. It plays a central role in the respiration and metabolism, and is the most important energy supplier in many enzymatic reactions. Its critical role as the energy storage molecule makes it extremely valuable to all...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-301

    authors: Mempin R,Tran H,Chen C,Gong H,Kim Ho K,Lu S

    更新日期:2013-12-24 00:00:00

  • Biphasic toxicodynamic features of some antimicrobial agents on microbial growth: a dynamic mathematical model and its implications on hormesis.

    abstract:BACKGROUND:In the present work, we describe a group of anomalous dose-response (DR) profiles and develop a dynamic model that is able to explain them. Responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (Carnobacterium piscicola and Leuc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-220

    authors: Murado MA,Vázquez JA

    更新日期:2010-08-19 00:00:00

  • In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice.

    abstract:BACKGROUND:Lactic acid bacteria (LAB) are major inhabitants and part of the normal microflora of the gastrointestinal tract (GIT) of humans and animals. Despite substantial evidence supporting the beneficial properties of LAB, only a few studies have addressed the migration and colonization of probiotic bacteria in the...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1315-4

    authors: Van Zyl WF,Deane SM,Dicks LMT

    更新日期:2018-10-30 00:00:00

  • Adaptive response to iterative passages of five Lactobacillus species in simulated vaginal fluid.

    abstract:BACKGROUND:Microbiome and metagenomic studies have given rise to a new understanding of microbial colonization of various human tissues and their ability to impact our health. One human microbiome growing in notoriety, the vaginal microbiome, stands out given its importance for women's health, and is peculiar in terms ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02027-8

    authors: Brandt K,Barrangou R

    更新日期:2020-11-10 00:00:00

  • The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli.

    abstract:BACKGROUND:The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1100-9

    authors: Ćudić E,Surmann K,Panasia G,Hammer E,Hunke S

    更新日期:2017-09-18 00:00:00

  • Colony shape as a genetic trait in the pattern-forming Bacillus mycoides.

    abstract:BACKGROUND:Bacillus mycoides Flügge, a Gram-positive, non-motile soil bacterium assigned to Bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (SIN or DX morphotypes). The molecular mechanism causing asymmetric curving is not known: ou...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-2-33

    authors: Di Franco C,Beccari E,Santini T,Pisaneschi G,Tecce G

    更新日期:2002-11-13 00:00:00

  • Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection.

    abstract:BACKGROUND:Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with em...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-240

    authors: França TG,Ishikawa LL,Zorzella-Pezavento SF,Chiuso-Minicucci F,Guerino CP,da Cunha Mde L,Sartori A

    更新日期:2009-11-23 00:00:00

  • 16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform.

    abstract:BACKGROUND:Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing p...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0738-z

    authors: Fouhy F,Clooney AG,Stanton C,Claesson MJ,Cotter PD

    更新日期:2016-06-24 00:00:00

  • Geochip-based analysis of microbial communities in alpine meadow soils in the Qinghai-Tibetan plateau.

    abstract:BACKGROUND:GeoChip 3.0, a microbial functional gene array, containing ~28,000 oligonucleotide probes and targeting ~57,000 sequences from 292 functional gene families, provided a powerful tool for researching microbial community structure in natural environments. The alpine meadow is a dominant plant community in the Q...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-72

    authors: Zhang Y,Lu Z,Liu S,Yang Y,He Z,Ren Z,Zhou J,Li D

    更新日期:2013-03-29 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-69

    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Arsenophonus, an emerging clade of intracellular symbionts with a broad host distribution.

    abstract:BACKGROUND:The genus Arsenophonus is a group of symbiotic, mainly insect-associated bacteria with rapidly increasing number of records. It is known from a broad spectrum of hosts and symbiotic relationships varying from parasitic son-killers to coevolving mutualists.The present study extends the currently known diversi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-143

    authors: Nováková E,Hypsa V,Moran NA

    更新日期:2009-07-20 00:00:00

  • The biological effects of microencapsulated organic acids and botanicals induces tissue-specific and dose-dependent changes to the Gallus gallus microbiota.

    abstract:BACKGROUND:Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02001-4

    authors: Feye KM,Swaggerty CL,Kogut MH,Ricke SC,Piva A,Grilli E

    更新日期:2020-11-02 00:00:00

  • A high throughput multiplex PCR assay for simultaneous detection of seven aminoglycoside-resistance genes in Enterobacteriaceae.

    abstract:BACKGROUND:The aminoglycoside-resistance genes encoding aminoglycoside modifying enzymes and 16S rRNA methyltransferases are main factors contributing to increasing resistance to aminoglycosides. Characterization and distribution of antimicrobial resistance gene profiles provide important information on the potential d...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-58

    authors: Hu X,Xu B,Yang Y,Liu D,Yang M,Wang J,Shen H,Zhou X,Ma X

    更新日期:2013-03-14 00:00:00

  • Laboratory divergence of Methylobacterium extorquens AM1 through unintended domestication and past selection for antibiotic resistance.

    abstract:BACKGROUND:A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discove...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-2

    authors: Carroll SM,Xue KS,Marx CJ

    更新日期:2014-01-02 00:00:00

  • Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens.

    abstract:BACKGROUND:The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS:In t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1440-8

    authors: Hazarika DJ,Goswami G,Gautom T,Parveen A,Das P,Barooah M,Boro RC

    更新日期:2019-04-02 00:00:00

  • Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.

    abstract:BACKGROUND:The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02057-2

    authors: Bai Y,Wang R,Song W,Li L,Santoferrara LF,Hu X

    更新日期:2020-12-14 00:00:00

  • Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination.

    abstract:BACKGROUND:Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-161

    authors: Carlos C,Pires MM,Stoppe NC,Hachich EM,Sato MI,Gomes TA,Amaral LA,Ottoboni LM

    更新日期:2010-06-01 00:00:00

  • Mechanistic studies of intracellular delivery of proteins by cell-penetrating peptides in cyanobacteria.

    abstract:BACKGROUND:The plasma membrane plays an essential role in selective permeability, compartmentalization, osmotic balance, and cellular uptake. The characteristics and functions of cyanobacterial membranes have been extensively investigated in recent years. Cell-penetrating peptides (CPPs) are special nanocarriers that c...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-57

    authors: Liu BR,Huang YW,Lee HJ

    更新日期:2013-03-14 00:00:00

  • Phenotypic and genotypic characterisation of multiple antibiotic-resistant Staphylococcus aureus exposed to subinhibitory levels of oxacillin and levofloxacin.

    abstract:BACKGROUND:The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern i...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0791-7

    authors: Jo A,Ahn J

    更新日期:2016-07-29 00:00:00

  • Influence of internalin A murinisation on host resistance to orally acquired listeriosis in mice.

    abstract:BACKGROUND:The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-90

    authors: Bergmann S,Beard PM,Pasche B,Lienenklaus S,Weiss S,Gahan CG,Schughart K,Lengeling A

    更新日期:2013-04-23 00:00:00

  • High-quality genome sequence assembly of R.A73 Enterococcus faecium isolated from freshwater fish mucus.

    abstract:BACKGROUND:Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that pr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01980-8

    authors: El Jeni R,Ghedira K,El Bour M,Abdelhak S,Benkahla A,Bouhaouala-Zahar B

    更新日期:2020-10-23 00:00:00

  • Analysis of and function predictions for previously conserved hypothetical or putative proteins in Blochmannia floridanus.

    abstract:BACKGROUND:There is an increasing interest to better understand endosymbiont capabilities in insects both from an ecological point of view and for pest control. Blochmannia floridanus provides important nutrients for its host, the ant Camponotus, while the bacterium in return is provided with a niche to proliferate. Bl...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-1

    authors: Gaudermann P,Vogl I,Zientz E,Silva FJ,Moya A,Gross R,Dandekar T

    更新日期:2006-01-09 00:00:00

  • Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast.

    abstract:BACKGROUND:Meiotic cells undergo two rounds of nuclear division and generate gametes. Previous studies have indicated that a number of transcription factors modulate the transcriptome in successive waves during meiosis and spore formation in fission yeast. However, the mechanisms underlying the post-transcriptional reg...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-188

    authors: Togashi N,Yamashita A,Sato M,Yamamoto M

    更新日期:2014-07-15 00:00:00

  • Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

    abstract:BACKGROUND:The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-63

    authors: Sachse K,Laroucau K,Hotzel H,Schubert E,Ehricht R,Slickers P

    更新日期:2008-04-17 00:00:00

  • A Candida albicans early stage biofilm detachment event in rich medium.

    abstract:BACKGROUND:Dispersal from Candida albicans biofilms that colonize catheters is implicated as a primary factor in the link between contaminated catheters and life threatening blood stream infections (BSI). Appropriate in vitro C. albicans biofilm models are needed to probe factors that induce detachment events. RESULTS...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-25

    authors: Sellam A,Al-Niemi T,McInnerney K,Brumfield S,Nantel A,Suci PA

    更新日期:2009-02-02 00:00:00

  • Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti.

    abstract:BACKGROUND:Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. Despite the wide distribution of these enzymes, their biological roles are unclear in part because the rea...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0780-x

    authors: Zhang Y,Smallbone LA,diCenzo GC,Morton R,Finan TM

    更新日期:2016-07-26 00:00:00

  • Differences between predicted outer membrane proteins of genotype 1 and 2 Mannheimia haemolytica.

    abstract:BACKGROUND:Mannheimia haemolytica strains isolated from North American cattle have been classified into two genotypes (1 and 2). Although members of both genotypes have been isolated from the upper and lower respiratory tracts of cattle with or without bovine respiratory disease (BRD), genotype 2 strains are much more ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01932-2

    authors: Clawson ML,Schuller G,Dickey AM,Bono JL,Murray RW,Sweeney MT,Apley MD,DeDonder KD,Capik SF,Larson RL,Lubbers BV,White BJ,Blom J,Chitko-McKown CG,Brichta-Harhay DM,Smith TPL

    更新日期:2020-08-12 00:00:00