Genomic heterogeneity of Dichelobacter nodosus within and between UK sheep flocks and between age groups within a flock.

Abstract:

BACKGROUND:Footrot and interdigital dermatitis are endemic infectious diseases in all sheep farming regions, impairing welfare and production. The development of efficacious vaccines against the primary causative pathogen has been hampered by the extensive antigenic diversity of Dichelobacter nodosus. Understanding the heterogeneity of the pathogen within and between flocks is essential if the feasibility of bespoke vaccine production is to be assessed for use in the U.K. RESULTS:In this study 56 ewe and lamb isolates from 9 flocks were compared by D. nodosus serogroup and Multi Locus Sequence Type which provides significantly enhanced discriminatory power for molecular epidemiology. Serogroup heterogeneity between flocks ranged from two to five unique serogroups per flock. Three flocks contained isolates of two serogroups, two flocks contained isolates of three serogroups and one flock included isolates of five serogroups. Analysis of 25 isolates from one flock with high prevalence of lameness, identified that serogroup and sequence type was significantly correlated with age. Significantly higher proportion of lambs were infected with serogroup B (principally ST85) as opposed to serogroup H (principally ST86), which predominated amongst adult sheep. CONCLUSIONS:Genomic heterogeneity of the pathogen was significantly lower within flock compared to heterogenicity observed between flocks. Furthermore, this study indicates that within a flock, the host-pathogen dynamics and susceptibility to particular D. nodosus strains may be age dependent.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Davies PL,Blanchard AM,Staley CE,Bollard NJ,Coffey TJ,Tötemeyer S

doi

10.1186/s12866-020-01769-9

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

107

issue

1

issn

1471-2180

pii

10.1186/s12866-020-01769-9

journal_volume

20

pub_type

杂志文章
  • Genomic analysis of methicillin-resistant Staphylococcus aureus strain SO-1977 from Sudan.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnost...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1470-2

    authors: Ali MS,Isa NM,Abedelrhman FM,Alyas TB,Mohammed SE,Ahmed AE,Ahmed ZSA,Lau NS,Garbi MI,Amirul AA,Seed AO,Omer RA,Mohamed SB

    更新日期:2019-06-11 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-69

    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Determination of antibacterial activity and minimum inhibitory concentration of larval extract of fly via resazurin-based turbidometric assay.

    abstract:BACKGROUND:Antimicrobial resistance is currently a major global issue. As the rate of emergence of antimicrobial resistance has superseded the rate of discovery and introduction of new effective drugs, the medical arsenal now is experiencing shortage of effective drugs to combat diseases, particularly against diseases ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-0936-3

    authors: Teh CH,Nazni WA,Nurulhusna AH,Norazah A,Lee HL

    更新日期:2017-02-16 00:00:00

  • Differences in distribution of MLS antibiotics resistance genes in clinical isolates of staphylococci belonging to species: S. epidermidis, S. hominis, S. haemolyticus, S. simulans and S. warneri.

    abstract:BACKGROUND:Macrolides and lincosamides are two leading types of antibiotics commonly used in therapies. The study examines the differences in resistance to these antibiotics and their molecular bases in S. epidermidis as well as in rarely isolated species of coagulase-negative staphylococci such as S. hominis, S. haemo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1496-5

    authors: Szemraj M,Czekaj T,Kalisz J,Szewczyk EM

    更新日期:2019-06-10 00:00:00

  • Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S.

    abstract:BACKGROUND:Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as i...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01863-y

    authors: Lefoulon E,Clark T,Borveto F,Perriat-Sanguinet M,Moulia C,Slatko BE,Gavotte L

    更新日期:2020-06-30 00:00:00

  • Using NanoSIMS coupled with microfluidics to visualize the early stages of coral infection by Vibrio coralliilyticus.

    abstract:BACKGROUND:Global warming has triggered an increase in the prevalence and severity of coral disease, yet little is known about coral/pathogen interactions in the early stages of infection. The point of entry of the pathogen and the route that they take once inside the polyp is currently unknown, as is the coral's capac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1173-0

    authors: Gibbin E,Gavish A,Domart-Coulon I,Kramarsky-Winter E,Shapiro O,Meibom A,Vardi A

    更新日期:2018-04-20 00:00:00

  • Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species.

    abstract:BACKGROUND:Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-250

    authors: Stone JK,Mayo M,Grasso SA,Ginther JL,Warrington SD,Allender CJ,Doyle A,Georgia S,Kaestli M,Broomall SM,Karavis MA,Insalaco JM,Hubbard KS,McNew LA,Gibbons HS,Currie BJ,Keim P,Tuanyok A

    更新日期:2012-11-05 00:00:00

  • Repertoire of the gut microbiota from stomach to colon using culturomics and next-generation sequencing.

    abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1304-7

    authors: Mailhe M,Ricaboni D,Vitton V,Gonzalez JM,Bachar D,Dubourg G,Cadoret F,Robert C,Delerce J,Levasseur A,Fournier PE,Angelakis E,Lagier JC,Raoult D

    更新日期:2018-10-24 00:00:00

  • Genome-wide transcriptome profiling of nitrogen fixation in Paenibacillus sp. WLY78.

    abstract:BACKGROUND:Diazotrophic (nitrogen-fixing) Gram-positive and endospore-formed Paenibacillus spp. have potential uses as a bacterial fertilizer in agriculture. The transcriptional analysis of nitrogen fixation in Paenibacillus is lacking, although regulation mechanisms of nitrogen fixation have been well studied in Gram-...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0642-6

    authors: Shi HW,Wang LY,Li XX,Liu XM,Hao TY,He XJ,Chen SF

    更新日期:2016-03-01 00:00:00

  • Identification of a Golgi-localized UDP-N-acetylglucosamine transporter in Trypanosoma cruzi.

    abstract:BACKGROUND:Nucleotide sugar transporters (NSTs) play an essential role in translocating nucleotide sugars into the lumen of the endoplasmic reticulum and Golgi apparatus to be used as substrates in glycosylation reactions. This intracellular transport is an essential step in the biosynthesis of glycoconjugates. RESULT...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0601-7

    authors: Baptista CG,Rodrigues EC,Morking P,Klinke A,Zardo ML,Soares MJ,de Aguiar AM,Goldenberg S,Ramos AS

    更新日期:2015-11-21 00:00:00

  • A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol.

    abstract:BACKGROUND:L-arabitol dehydrogenase (LAD) and xylitol dehydrogenase (XDH) are involved in the degradation of L-arabinose and D-xylose, which are among the most abundant monosaccharides on earth. Previous data demonstrated that LAD and XDH not only differ in the activity on their biological substrate, but also that only...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-166

    authors: Rutten L,Ribot C,Trejo-Aguilar B,Wösten HA,de Vries RP

    更新日期:2009-08-12 00:00:00

  • Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis.

    abstract:BACKGROUND:Alpha-isopropylmalate synthase (alpha-IPMS) is the key enzyme that catalyzes the first committed step in the leucine biosynthetic pathway. The gene encoding alpha-IPMS in Mycobacterium tuberculosis, leuA, is polymorphic due to the insertion of 57-bp repeat units referred to as Variable Number of Tandem Repea...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-122

    authors: Yindeeyoungyeon W,Likitvivatanavong S,Palittapongarnpim P

    更新日期:2009-06-09 00:00:00

  • Computational approaches for discovery of common immunomodulators in fungal infections: towards broad-spectrum immunotherapeutic interventions.

    abstract:BACKGROUND:Fungi are the second most abundant type of human pathogens. Invasive fungal pathogens are leading causes of life-threatening infections in clinical settings. Toxicity to the host and drug-resistance are two major deleterious issues associated with existing antifungal agents. Increasing a host's tolerance and...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-224

    authors: Kidane YH,Lawrence C,Murali TM

    更新日期:2013-10-07 00:00:00

  • Extracellular DNA release confers heterogeneity in Candida albicans biofilm formation.

    abstract:BACKGROUND:Biofilm formation by Candida albicans has shown to be highly variable and is directly associated with pathogenicity and poor clinical outcomes in patients at risk. The aim of this study was to test the hypotheses that the extracellular DNA release by C. albicans is strain dependent and is associated with bio...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0303-6

    authors: Rajendran R,Sherry L,Lappin DF,Nile CJ,Smith K,Williams C,Munro CA,Ramage G

    更新日期:2014-12-05 00:00:00

  • Chlamydophila pneumoniae derived from inclusions late in the infectious cycle induce aponecrosis in human aortic endothelial cells.

    abstract:BACKGROUND:Atherosclerosis is still the leading cause of death in the western world. Besides known risk factors studies demonstrating Chlamydophila pneumoniae (C. pneumoniae) to be implicated in the progression of the disease, little is known about C. pneumoniae infection dynamics. We investigated whether C. pneumoniae...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-32

    authors: Marino J,Stoeckli I,Walch M,Latinovic-Golic S,Sundstroem H,Groscurth P,Ziegler U,Dumrese C

    更新日期:2008-02-19 00:00:00

  • Protein expression dynamics during Escherichia coli glucose-lactose diauxie.

    abstract:BACKGROUND:Escherichia coli is a well-studied anaerobic bacteria which is able to regulate metabolic pathways depending on the type of sugar presented in the medium. We have studied the glucose-lactose shift in E. coli at the protein level using a recently developed mass spectrometry platform. METHOD:Cells were grown ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-126

    authors: Mostovenko E,Deelder AM,Palmblad M

    更新日期:2011-06-01 00:00:00

  • Functional analysis of an intergenic non-coding sequence within mce1 operon of M.tuberculosis.

    abstract:BACKGROUND:The mce operons play an important role in the entry of M. tuberculosis into macrophages and non-phagocytic cells. Their non-redundant function as well as complex regulation is implied by the phenotype of mce mutants. Recently, mce1 operon was found to extend over 13 genes, fadD5 (Rv0166) being the first gene...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-128

    authors: Joon M,Bhatia S,Pasricha R,Bose M,Brahmachari V

    更新日期:2010-04-27 00:00:00

  • Novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of Patagonia.

    abstract:BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. Our knowledge of PAH-degrading bacterial populations in the marine environment is still very limited, and mainly originat...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-50

    authors: Lozada M,Riva Mercadal JP,Guerrero LD,Di Marzio WD,Ferrero MA,Dionisi HM

    更新日期:2008-03-25 00:00:00

  • Staphylococcus aureus biofilm formation at the physiologic glucose concentration depends on the S. aureus lineage.

    abstract:BACKGROUND:Since bacteria embedded in biofilms are far more difficult to eradicate than planktonic infections, it would be useful to know whether certain Staphylococcus aureus lineages are especially involved in strong biofilm formation. For this reason, in vitro biofilm formation of 228 clinical S. aureus isolates of ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-229

    authors: Croes S,Deurenberg RH,Boumans ML,Beisser PS,Neef C,Stobberingh EE

    更新日期:2009-10-28 00:00:00

  • Ultrastructure and molecular phylogenetic position of a novel euglenozoan with extrusive episymbiotic bacteria: Bihospites bacati n. gen. et sp. (Symbiontida).

    abstract:BACKGROUND:Poorly understood but highly diverse microbial communities exist within anoxic and oxygen-depleted marine sediments. These communities often harbour single-celled eukaryotes that form symbiotic associations with different prokaryotes. During low tides in South-western British Columbia, Canada, vast areas of ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-145

    authors: Breglia SA,Yubuki N,Hoppenrath M,Leander BS

    更新日期:2010-05-19 00:00:00

  • Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti.

    abstract:BACKGROUND:Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. Despite the wide distribution of these enzymes, their biological roles are unclear in part because the rea...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0780-x

    authors: Zhang Y,Smallbone LA,diCenzo GC,Morton R,Finan TM

    更新日期:2016-07-26 00:00:00

  • Small molecule inhibitors of the Yersinia type III secretion system impair the development of Chlamydia after entry into host cells.

    abstract:BACKGROUND:Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epitheli...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-75

    authors: Muschiol S,Normark S,Henriques-Normark B,Subtil A

    更新日期:2009-04-21 00:00:00

  • The canine isolate Lactobacillus acidophilus LAB20 adheres to intestinal epithelium and attenuates LPS-induced IL-8 secretion of enterocytes in vitro.

    abstract:BACKGROUND:For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The strain Lactobacillus acidophilus LAB20 was isolated from the jejunum of a healthy dog, where it was found to be t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0337-9

    authors: Kainulainen V,Tang Y,Spillmann T,Kilpinen S,Reunanen J,Saris PE,Satokari R

    更新日期:2015-01-16 00:00:00

  • Biological characterization of Pasteurella multocida present in the Saiga population.

    abstract:BACKGROUND:This study provides biochemical and molecular genetic characteristics of P. multocida isolated from dead saigas in 1988, 2010-2015 on the territory of the Republic of Kazakhstan. RESULTS:Bacteriological samples taken from carcasses of saiga antelope during mortality events recorded in West Kazakhstan in bot...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1407-9

    authors: Orynbayev M,Sultankulova K,Sansyzbay A,Rystayeva R,Shorayeva K,Namet A,Fereidouni S,Ilgekbayeva G,Barakbayev K,Kopeyev S,Kock R

    更新日期:2019-02-11 00:00:00

  • Cow manure application effectively regulates the soil bacterial community in tea plantation.

    abstract:BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01871-y

    authors: Zhang S,Sun L,Wang Y,Fan K,Xu Q,Li Y,Ma Q,Wang J,Ren W,Ding Z

    更新日期:2020-07-01 00:00:00

  • Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.

    abstract:BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-256

    authors: Tazumi A,Kakinuma Y,Misawa N,Moore JE,Millar BC,Matsuda M

    更新日期:2009-12-11 00:00:00

  • A Candida albicans early stage biofilm detachment event in rich medium.

    abstract:BACKGROUND:Dispersal from Candida albicans biofilms that colonize catheters is implicated as a primary factor in the link between contaminated catheters and life threatening blood stream infections (BSI). Appropriate in vitro C. albicans biofilm models are needed to probe factors that induce detachment events. RESULTS...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-25

    authors: Sellam A,Al-Niemi T,McInnerney K,Brumfield S,Nantel A,Suci PA

    更新日期:2009-02-02 00:00:00

  • Design of PCR assays to specifically detect and identify 37 Lactobacillus species in a single 96 well plate.

    abstract:BACKGROUND:Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene seque...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01781-z

    authors: Kim E,Yang SM,Lim B,Park SH,Rackerby B,Kim HY

    更新日期:2020-04-15 00:00:00

  • Virulence regulator AphB enhances toxR transcription in Vibrio cholerae.

    abstract:BACKGROUND:Vibrio cholerae is the causative agent of cholera. Extensive studies reveal that complicated regulatory cascades regulate expression of virulence genes, the products of which are required for V. cholerae to colonize and cause disease. In this study, we investigated the expression of the key virulence regulat...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-3

    authors: Xu X,Stern AM,Liu Z,Kan B,Zhu J

    更新日期:2010-01-06 00:00:00

  • Young «oil site» of the Uzon Caldera as a habitat for unique microbial life.

    abstract:BACKGROUND:The Uzon Caldera is one of the places on our planet with unique geological, ecological, and microbiological characteristics. Uzon oil is the youngest on Earth. Uzon oil has unique composition, with low proportion of heavy fractions and relatively high content of saturated hydrocarbons. Microbial communities ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02012-1

    authors: Peltek SE,Bryanskaya AV,Uvarova YE,Rozanov AS,Ivanisenko TV,Ivanisenko VA,Lazareva EV,Saik OV,Efimov VM,Zhmodik SM,Taran OP,Slynko NM,Shekhovtsov SV,Parmon VN,Dobretsov NL,Kolchanov NA

    更新日期:2020-11-24 00:00:00