Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena.

abstract：BACKGROUND:Microbial biofilms are known to cause an increasing number of chronic inflammatory and infectious conditions. A classical example is chronic periodontal disease, a condition initiated by the subgingival dental plaque biofilm on gingival epithelial tissues. We describe here a new model that permits the examin...

journal_title：BMC microbiology

authors： Guggenheim B,Gmür R,Galicia JC,Stathopoulou PG,Benakanakere MR,Meier A,Thurnheer T,Kinane DF

更新日期：2009-12-31 00:00:00

abstract：BACKGROUND:It is well known that expression of certain bacterial genes responds rapidly to such stimuli as exposure to toxic chemicals and physical agents. It is generally believed that the proteins encoded in these genes are important for successful survival of the organism under the hostile conditions. Analogously, t...

journal_title：BMC microbiology

authors： Yamaguchi Y,Tomoyasu T,Takaya A,Morioka M,Yamamoto T

更新日期：2003-08-12 00:00:00

abstract：BACKGROUND:The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS:We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phen...

journal_title：BMC microbiology

authors： Morrison SS,Mullineaux CW,Ashby MK

更新日期：2005-08-02 00:00:00

abstract：BACKGROUND:Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use o...

journal_title：BMC microbiology

authors： Miranda C,Bettencourt S,Pozdniakova T,Pereira J,Sampaio P,Franco-Duarte R,Pais C

更新日期：2020-03-14 00:00:00

abstract：BACKGROUND:Francisella tularensis is a fastidious, Gram-negative coccobacillus and is the causative agent of tularemia. To assess viability yet overcome lengthy incubation periods, a culture-based PCR method was used to detect early growth of the lowest possible number of F. tularensis cells. This method utilized a pre...

journal_title：BMC microbiology

authors： Buse HY,Morris BJ,Rice EW

更新日期：2020-03-25 00:00:00

abstract：BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnost...

journal_title：BMC microbiology

authors： Ali MS,Isa NM,Abedelrhman FM,Alyas TB,Mohammed SE,Ahmed AE,Ahmed ZSA,Lau NS,Garbi MI,Amirul AA,Seed AO,Omer RA,Mohamed SB

更新日期：2019-06-11 00:00:00

abstract：BACKGROUND:During the last two decades research on animal filarial parasites, especially Onchocerca ochengi, infecting cattle in savanna areas of Africa revealed that O. ochengi as an animal model has biological features that are similar to those of O. volvulus, the aetiological agent of human onchocerciasis. There is,...

journal_title：BMC microbiology

authors： Ngwasiri NN,Brattig NW,Ndjonka D,Liebau E,Paguem A,Leusder D,Kingsley MT,Eisenbarth A,Renz A,Daniel AM

更新日期：2021-01-06 00:00:00

abstract：BACKGROUND:The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by...

journal_title：BMC microbiology

authors： Ainsaar K,Mumm K,Ilves H,Hõrak R

更新日期：2014-06-20 00:00:00

abstract：BACKGROUND:Cryptosporidium spp. and Giardia duodenalis are important causes of diarrheal diseases in humans and animals worldwide, and there is an increased interest in the role of animals in the mechanical transmission of these protozoa. To examine the role of yaks in this process, we examined the occurrence and genot...

journal_title：BMC microbiology

authors： Qi M,Cai J,Wang R,Li J,Jian F,Huang J,Zhou H,Zhang L

更新日期：2015-05-21 00:00:00

abstract：BACKGROUND:Brucellosis incidence in China is divided into three stages: high incidence (1950s-1960s), decline (1970s-1980s), and re-emergence (1990s-2010s). At the re-emergence stage, Brucellosis incidence grew exponentially and spread to all 32 provinces. We describe the magnitude and the etiological distribution chan...

journal_title：BMC microbiology

authors： Piao DR,Liu X,Di DD,Xiao P,Zhao ZZ,Xu LQ,Tian GZ,Zhao HY,Fan WX,Cui BY,Jiang H

更新日期：2018-01-10 00:00:00

abstract：BACKGROUND:Enterobacter sakazakii is an emergent pathogen associated with ingestion of infant formula and accurate identification is important in both industrial and clinical settings. Bacterial species can be difficult to accurately characterise from complex biochemical datasets and computer algorithms can potentially...

journal_title：BMC microbiology

authors： Iversen C,Lancashire L,Waddington M,Forsythe S,Ball G

更新日期：2006-03-13 00:00:00

abstract：BACKGROUND:Pneumococcus is exposed to a variety of temperature and oxygen levels in the upper respiratory tract and as it invades the lung, tissues, and blood. We sought to determine the effect of environmental variability on growth in vitro and to assess variability between strains. We evaluated the effect of temperat...

journal_title：BMC microbiology

authors： Tóthpál A,Desobry K,Joshi SS,Wyllie AL,Weinberger DM

更新日期：2019-12-26 00:00:00

abstract：BACKGROUND:Streptococcus suis is a major swine pathogen worldwide that causes meningitis, septicemia, arthritis, and endocarditis. Using animal models, a surface-associated subtilisin-like protease (SspA) has recently been shown to be an important virulence factor for S. suis. In this study, we hypothesized that the S....

journal_title：BMC microbiology

authors： Bonifait L,Grenier D

更新日期：2011-03-01 00:00:00

abstract：BACKGROUND:Alpha-isopropylmalate synthase (alpha-IPMS) is the key enzyme that catalyzes the first committed step in the leucine biosynthetic pathway. The gene encoding alpha-IPMS in Mycobacterium tuberculosis, leuA, is polymorphic due to the insertion of 57-bp repeat units referred to as Variable Number of Tandem Repea...

journal_title：BMC microbiology

authors： Yindeeyoungyeon W,Likitvivatanavong S,Palittapongarnpim P

更新日期：2009-06-09 00:00:00

abstract：BACKGROUND:Xanthophyllomyces dendrorhous is a basidiomycetous yeast that synthesizes astaxanthin, a carotenoid with great biotechnological impact. The ergosterol and carotenoid synthetic pathways derive from the mevalonate pathway and involve cytochrome P450 enzymes. Among these enzymes, the CYP51 family, which is invo...

journal_title：BMC microbiology

authors： Leiva K,Werner N,Sepúlveda D,Barahona S,Baeza M,Cifuentes V,Alcaíno J

更新日期：2015-04-25 00:00:00

abstract：BACKGROUND:Leptospires lack many of the homologs for oxidative defense present in other bacteria, but do encode homologs of the Bacteriodes aerotolerance (Bat) proteins, which have been proposed to fulfill this function. Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific functi...

journal_title：BMC microbiology

authors： Stewart PE,Carroll JA,Dorward DW,Stone HH,Sarkar A,Picardeau M,Rosa PA

更新日期：2012-12-13 00:00:00

abstract：BACKGROUND:Streptococcus agalactiae or Group B Streptococci (GBS) have the ability to access various host sites, which reflects its adaptability to different environments during the course of infection. This adaptation is due to the expression of virulence factors that are involved with survival, invasion and bacterial...

journal_title：BMC microbiology

authors： Otaguiri ES,Morguette AE,Tavares ER,dos Santos PM,Morey AT,Cardoso JD,Perugini MR,Yamauchi LM,Yamada-Ogatta SF

更新日期：2013-12-21 00:00:00

abstract：BACKGROUND:Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antib...

journal_title：BMC microbiology

authors： Ma Y,Sun J,Gu L,Bao H,Zhao Y,Shi L,Yao W,Tian G,Wang X,Chen H

更新日期：2017-09-11 00:00:00

abstract：BACKGROUND:The Gram-negative bacterium Erwinia amylovora is the causal agent of the devastating disease fire blight in rosaceous plants such as apple, pear, quince, raspberry, and cotoneaster. In order to survive and multiply in a host, microbes must be able to circumvent the toxic effects of antimicrobial plant compou...

journal_title：BMC microbiology

authors： Pletzer D,Weingart H

更新日期：2014-07-11 00:00:00

abstract：BACKGROUND:Pseudomonas syringae is pathogenic to a large number of plant species. For host colonization and disease progression, strains of this bacterium utilize an array of type III-secreted effectors and other virulence factors, including small secreted molecules such as syringolin A, a peptide derivative that inhib...

journal_title：BMC microbiology

authors： Dudnik A,Dudler R

更新日期：2014-12-04 00:00:00

abstract：:Protein secretion plays a central role in modulating the interactions of bacteria with their environments. This is particularly the case when symbiotic bacteria (whether pathogenic, commensal or mutualistic) are interacting with larger host organisms. In the case of Gram-negative bacteria, secretion requires transloca...

journal_title：BMC microbiology

authors： Tseng TT,Tyler BM,Setubal JC

更新日期：2009-02-19 00:00:00

abstract：BACKGROUND:The balanced synthesis of membrane phospholipids, fatty acids and cell wall constituents is a vital facet of bacterial physiology, but there is little known about the biochemical control points that coordinate these activities in Gram-positive bacteria. In Escherichia coli, the glycerol-phosphate acyltransfe...

journal_title：BMC microbiology

authors： Parsons JB,Yao J,Jackson P,Frank M,Rock CO

更新日期：2013-11-16 00:00:00

abstract：BACKGROUND:The compatible solute trehalose is involved in the osmostress response of Rhizobium etli, the microsymbiont of Phaseolus vulgaris. In this work, we reconstructed trehalose metabolism in R. etli, and investigated its role in cellular adaptation and survival to heat and desiccation stress under free living con...

journal_title：BMC microbiology

authors： Reina-Bueno M,Argandoña M,Nieto JJ,Hidalgo-García A,Iglesias-Guerra F,Delgado MJ,Vargas C

更新日期：2012-09-17 00:00:00

abstract：BACKGROUND:Bacillus sp. H2O-1, isolated from the connate water of a Brazilian reservoir, produces an antimicrobial substance (denoted as AMS H2O-1) that is active against sulfate reducing bacteria, which are the major bacterial group responsible for biogenic souring and biocorrosion in petroleum reservoirs. Thus, the u...

journal_title：BMC microbiology

authors： Korenblum E,de Araujo LV,Guimarães CR,de Souza LM,Sassaki G,Abreu F,Nitschke M,Lins U,Freire DM,Barreto-Bergter E,Seldin L

更新日期：2012-11-07 00:00:00

abstract：BACKGROUND:Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. Despite the wide distribution of these enzymes, their biological roles are unclear in part because the rea...

journal_title：BMC microbiology

authors： Zhang Y,Smallbone LA,diCenzo GC,Morton R,Finan TM

更新日期：2016-07-26 00:00:00

abstract：BACKGROUND:Helicobacter pylori has diverged in parallel to its human host, leading to distinct phylogeographic populations. Recent evidence suggests that in the current human mixing in Latin America, European H. pylori (hpEurope) are increasingly dominant at the expense of Amerindian haplotypes (hspAmerind). This pheno...

journal_title：BMC microbiology

authors： Maldonado-Contreras A,Mane SP,Zhang XS,Pericchi L,Alarcón T,Contreras M,Linz B,Blaser MJ,Domínguez-Bello MG

更新日期：2013-09-19 00:00:00

abstract：BACKGROUND:Nucleotide sugar transporters (NSTs) play an essential role in translocating nucleotide sugars into the lumen of the endoplasmic reticulum and Golgi apparatus to be used as substrates in glycosylation reactions. This intracellular transport is an essential step in the biosynthesis of glycoconjugates. RESULT...

journal_title：BMC microbiology

authors： Baptista CG,Rodrigues EC,Morking P,Klinke A,Zardo ML,Soares MJ,de Aguiar AM,Goldenberg S,Ramos AS

更新日期：2015-11-21 00:00:00

abstract：BACKGROUND:Mycoplasmas-contamination of Orientia tsutsugamushi, one of the obligated intracellular bacteria, is a very serious problem in in vitro studies using cell cultures because mycoplasmas have significant influence on the results of scientific studies. Only a recommended decontamination method is to passage the ...

journal_title：BMC microbiology

authors： Ogawa M,Uchiyama T,Satoh M,Ando S

更新日期：2013-02-08 00:00:00

abstract：BACKGROUND:Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geo...

journal_title：BMC microbiology

authors： Leonard SR,Lacher DW,Lampel KA

更新日期：2015-08-25 00:00:00

abstract：BACKGROUND:Biofilms are ubiquitous. For instance, the majority of medical infections are thought to involve biofilms. However even after decades of investigation, the in vivo efficacy of many antimicrobial strategies is still debated suggesting there is a need for better understanding of biofilm antimicrobial tolerance...

journal_title：BMC microbiology

authors： Zuroff TR,Bernstein H,Lloyd-Randolfi J,Jimenez-Taracido L,Stewart PS,Carlson RP

更新日期：2010-07-07 00:00:00