Abstract：
BACKGROUND:Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxygen restriction, was compared in vitro and in vivo. Fluorescent protein labels were applied to allow certain identification of Listeria cells from untagged bacteria in in vivo samples, and to distinguish between cells grown under different conditions in mixed infection experiments. RESULTS:Infection of Caco-2 cells revealed that Listeria cultivated under oxygen-restricted conditions were approximately 100 fold more invasive than similar cultures grown without oxygen restriction. This was observed for exponentially growing bacteria, as well as for stationary-phase cultures. Oral dosage of guinea pigs with Listeria resulted in a significantly higher prevalence (p < 0.05) of these bacteria in jejunum, liver and spleen four and seven days after challenge, when the bacterial cultures had been grown under oxygen-restricted conditions prior to dosage. Additionally, a 10-100 fold higher concentration of Listeria in fecal samples was observed after dosage with oxygen-restricted bacteria. These differences were seen after challenge with single Listeria cultures, as well as with a mixture of two cultures grown with and without oxygen restriction. CONCLUSION:Our results show for the first time that the environmental conditions to which L. monocytogenes is exposed prior to ingestion are decisive for its in vivo infective potential in the gastrointestinal tract after passage of the gastric barrier. This is highly relevant for safety assessment of this organism in food.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Bo Andersen J,Roldgaard BB,Christensen BB,Licht TRdoi
10.1186/1471-2180-7-55subject
Has Abstractpub_date
2007-06-14 00:00:00pages
55issn
1471-2180pii
1471-2180-7-55journal_volume
7pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract：BACKGROUND:Neisseria meningitidis serogroups W and Y are the most common serogroups causing invasive meningococcal disease in Sweden. The majority of cases are caused by the serogroup W UK 2013 strain of clonal complex (cc) 11, and subtype 1 of the serogroup Y, YI strain of cc23. In this study, virulence factors of sev...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01760-4
更新日期：2020-04-15 00:00:00
abstract：BACKGROUND:Iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake and homeostasis. Information on iron management mechanisms is available for organisms living at circumneutral pH. However, very little is known about how...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-203
更新日期：2008-11-24 00:00:00
abstract：BACKGROUND:Enterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (HAIs). It is believed that the recent evolution of two clinically relevant species, Enterococcus faecalis and Enterococcus faecium occurred in a big part in a hospital env...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-66
更新日期：2014-03-14 00:00:00
abstract：BACKGROUND:Streptococcus pseudopneumoniae, is a novel member of the genus Streptococcus, falling close to related members like S. pneumoniae, S. mitis, and S. oralis. Its recent appearance has shed light on streptococcal infections, which has been unclear till recently. In this study, the transcriptome of S. pseudopneu...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-77
更新日期：2012-07-06 00:00:00
abstract：BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01871-y
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Probiotics are increasingly applied to prevent and treat a range of infectious, immune related and gastrointestinal diseases. Despite this, the mechanisms behind the putative effects of probiotics are poorly understood. One of the suggested modes of probiotic action is modulation of the endogenous gut microb...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1090-7
更新日期：2017-08-17 00:00:00
abstract：BACKGROUND:Iron plays important roles in the growth, reproduction and pathogenicity of Aeromonas hydrophila. In this study, we detected and compared the mRNA and protein expression profiles of A. hydrophila under normal and iron restricted medium with 200 μM 2,2-Dipyridyl using RNA Sequencing (RNA-seq) and isobaric tag...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1178-8
更新日期：2018-06-04 00:00:00
abstract：BACKGROUND:Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of fo...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-83
更新日期：2010-03-17 00:00:00
abstract：BACKGROUND:Flavobacterium columnare (Bacteroidetes) is the causative agent of columnaris disease in farmed freshwater fish around the world. The bacterium forms three colony morphotypes (Rhizoid, Rough and Soft), but the differences of the morphotypes are poorly known. We studied the virulence of the morphotypes produc...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-170
更新日期：2014-06-26 00:00:00
abstract：BACKGROUND:Leptospires lack many of the homologs for oxidative defense present in other bacteria, but do encode homologs of the Bacteriodes aerotolerance (Bat) proteins, which have been proposed to fulfill this function. Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific functi...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-290
更新日期：2012-12-13 00:00:00
abstract：BACKGROUND:In the present work, we describe a group of anomalous dose-response (DR) profiles and develop a dynamic model that is able to explain them. Responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (Carnobacterium piscicola and Leuc...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-220
更新日期：2010-08-19 00:00:00
abstract：BACKGROUND:Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. The exten...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-58
更新日期：2009-03-18 00:00:00
abstract：BACKGROUND:Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evi...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1129-9
更新日期：2017-11-22 00:00:00
abstract：BACKGROUND:Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacteria...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1184-x
更新日期：2018-05-08 00:00:00
abstract：BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-218
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking mor...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-91
更新日期：2011-05-04 00:00:00
abstract：BACKGROUND:Pneumococcus is exposed to a variety of temperature and oxygen levels in the upper respiratory tract and as it invades the lung, tissues, and blood. We sought to determine the effect of environmental variability on growth in vitro and to assess variability between strains. We evaluated the effect of temperat...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1671-8
更新日期：2019-12-26 00:00:00
abstract：BACKGROUND:Successful viral infection requires the involvement of host cellular factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by numerous viruses to promote the folding, maturation, or assembly of viral proteins. We have previously shown that HSP70 is significantly elevated in porcine repr...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-64
更新日期：2014-03-13 00:00:00
abstract：BACKGROUND:The increase in resistance to antibiotics among disease-causing bacteria necessitates the development of alternative antimicrobial approaches such as the use of light-activated antimicrobial agents (LAAAs). Light of an appropriate wavelength activates the LAAA to produce cytotoxic species which can then caus...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-111
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Changes in aboveground plant species diversity as well as variations of environmental conditions such as exposure of ecosystems to elevated concentrations of atmospheric carbon dioxide may lead to changes in metabolic activity, composition and diversity of belowground microbial communities, both bacterial an...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-6-68
更新日期：2006-07-27 00:00:00
abstract：BACKGROUND:In this study, we have evaluated the immunological status of hepatitis C virus (HCV)-infected patients aiming at identifying putative biomarkers associated with distinct degrees of liver fibrosis. Peripheral blood and tissue T-cells as well as cytokine levels were quantified by flow cytometry. RESULTS:Data ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-015-0610-6
更新日期：2016-01-07 00:00:00
abstract：BACKGROUND:Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-7-14
更新日期：2007-03-05 00:00:00
abstract：BACKGROUND:Biofilms are ubiquitous. For instance, the majority of medical infections are thought to involve biofilms. However even after decades of investigation, the in vivo efficacy of many antimicrobial strategies is still debated suggesting there is a need for better understanding of biofilm antimicrobial tolerance...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-185
更新日期：2010-07-07 00:00:00
abstract：BACKGROUND:The RNA interference (RNAi) pathway acts as an innate antiviral immune response in Aedes aegypti, modulating arbovirus infection of mosquitoes. Sindbis virus (SINV; family: Togaviridae, genus: Alphavirus) is an arbovirus that infects Ae. aegypti in the laboratory. SINV strain TR339 encounters a midgut escape...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-130
更新日期：2010-04-28 00:00:00
abstract：BACKGROUND:Susceptibility to Bordetella pertussis infection varies widely. These differences can partly be explained by genetic host factors. HcB-28 mice are more resistant to B. pertussis infection than C3H mice, which could partially be ascribed to the B. pertussis susceptibility locus-1 (Bps1) on chromosome 12. The ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-7-88
更新日期：2007-10-12 00:00:00
abstract：BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1318-1
更新日期：2018-10-22 00:00:00
abstract：BACKGROUND:Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with em...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-240
更新日期：2009-11-23 00:00:00
abstract：BACKGROUND:Several intracellular bacterial pathogens have evolved subtle strategies to subvert vesicular trafficking pathways of their host cells to avoid killing and to replicate inside the cells. Brucellae are Gram-negative facultative intracellular bacteria that are responsible for brucellosis, a worldwide extended ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-014-0223-5
更新日期：2014-09-02 00:00:00
abstract：BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dep...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-016-0813-5
更新日期：2016-08-26 00:00:00
abstract：BACKGROUND:Antimicrobial helper-compounds may reverse antimicrobial resistance. Sertraline, a antidepressant drug, has been suggested as a tetracycline helper-compound. Tetracycline is the preferred antimicrobial for treatment of enteric diseases in pigs. This study is the first to evaluate the potency of sertraline as...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1383-5
更新日期：2019-01-11 00:00:00
