Modeling the growth dynamics of multiple Escherichia coli strains in the pig intestine following intramuscular ampicillin treatment.

Abstract:

BACKGROUND:This study evaluated how dosing regimen for intramuscularly-administered ampicillin, composition of Escherichia coli strains with regard to ampicillin susceptibility, and excretion of bacteria from the intestine affected the level of resistance among Escherichia coli strains in the intestine of nursery pigs. It also examined the dynamics of the composition of bacterial strains during and after the treatment. The growth responses of strains to ampicillin concentrations were determined using in vitro growth curves. Using these results as input data, growth predictions were generated using a mathematical model to simulate the competitive growth of E. coli strains in a pig intestine under specified plasma concentration profiles of ampicillin. RESULTS:In vitro growth results demonstrated that the resistant strains did not carry a fitness cost for their resistance, and that the most susceptible strains were more affected by increasing concentrations of antibiotics that the rest of the strains. The modeling revealed that short treatment duration resulted in lower levels of resistance and that dosing frequency did not substantially influence the growth of resistant strains. Resistance levels were found to be sensitive to the number of competing strains, and this effect was enhanced by longer duration of treatment. High excretion of bacteria from the intestine favored resistant strains over sensitive strains, but at the same time it resulted in a faster return to pre-treatment levels after the treatment ended. When the duration of high excretion was set to be limited to the treatment time (i.e. the treatment was assumed to result in a cure of diarrhea) resistant strains required longer time to reach the previous level. CONCLUSION:No fitness cost was found to be associated with ampicillin resistance in E. coli. Besides dosing factors, epidemiological factors (such as number of competing strains and bacterial excretion) influenced resistance development and need to be considered further in relation to optimal treatment strategies. The modeling approach used in the study is generic, and could be used for prediction of the effect of treatment with other drugs and other administration routes for effect on resistance development in the intestine of pigs.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Ahmad A,Zachariasen C,Christiansen LE,Græsbøll K,Toft N,Matthews L,Nielsen SS,Olsen JE

doi

10.1186/s12866-016-0823-3

subject

Has Abstract

pub_date

2016-09-06 00:00:00

pages

205

issue

1

issn

1471-2180

pii

10.1186/s12866-016-0823-3

journal_volume

16

pub_type

杂志文章
  • Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli.

    abstract:BACKGROUND:Alpha (alpha)-hemolysin is a pore forming cytolysin and serves as a virulence factor in intestinal and extraintestinal pathogenic strains of E. coli. It was suggested that the genes encoding alpha-hemolysin (hlyCABD) which can be found on the chromosome and plasmid, were acquired through horizontal gene tran...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-193

    authors: Burgos Y,Beutin L

    更新日期:2010-07-19 00:00:00

  • Outer membrane vesicles of Helicobacter pylori TK1402 are involved in biofilm formation.

    abstract:BACKGROUND:Helicobacter pylori forms biofilms on glass surfaces at the air-liquid interface in in vitro batch cultures; however, biofilms of H. pylori have not been well characterized. In the present study, we analyzed the ability of H. pylori strains to form biofilms and characterized the underlying mechanisms of H. p...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-197

    authors: Yonezawa H,Osaki T,Kurata S,Fukuda M,Kawakami H,Ochiai K,Hanawa T,Kamiya S

    更新日期:2009-09-15 00:00:00

  • The ColRS signal transduction system responds to the excess of external zinc, iron, manganese, and cadmium.

    abstract:BACKGROUND:The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-162

    authors: Ainsaar K,Mumm K,Ilves H,Hõrak R

    更新日期:2014-06-20 00:00:00

  • Antibiosis interaction of Staphylococccus aureus on Aspergillus fumigatus assessed in vitro by mixed biofilm formation.

    abstract:BACKGROUND:Microorganisms of different species interact in several ecological niches, even causing infection. During the infectious process, a biofilm of single or multispecies can develop. Aspergillus fumigatus and Staphyloccocus aureus are etiologic agents that can cause infectious keratitis. We analyzed in vitro sin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0363-2

    authors: Ramírez Granillo A,Canales MG,Espíndola ME,Martínez Rivera MA,de Lucio VM,Tovar AV

    更新日期:2015-02-15 00:00:00

  • Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S.

    abstract:BACKGROUND:Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as i...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01863-y

    authors: Lefoulon E,Clark T,Borveto F,Perriat-Sanguinet M,Moulia C,Slatko BE,Gavotte L

    更新日期:2020-06-30 00:00:00

  • Characterization of the interaction of staphylococcal enterotoxin B with CD1d expressed in human renal proximal tubule epithelial cells.

    abstract:BACKGROUND:Participation of renal cells in the pathogenesis of staphylococcal enterotoxin B (SEB) is critical for late cleansing and sequestration of the antigens facilitated by CD1d mediated antigen sensing and recognition. This is a noted deviation from the typical antigen recognition process that recruits the major ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0344-5

    authors: Hammamieh R,Chakraborty N,Lin Y,Shupp JW,Miller SA,Morris S,Jett M

    更新日期:2015-02-04 00:00:00

  • ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum.

    abstract:BACKGROUND:Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS:We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-0932-7

    authors: Schmidpeter J,Dahl M,Hofmann J,Koch C

    更新日期:2017-01-19 00:00:00

  • Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrnA-P1.

    abstract:BACKGROUND:The ability of an intracellular pathogen to establish infection depends on the capacity of the organism to survive and replicate inside the host. Mycobacterium fortuitum is a bacteria that contains genes involved in the detoxification of the oxygen reactive species such as those produced by the host during t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-100

    authors: Núñez MC,Menéndez MC,Rebollo MJ,García MJ

    更新日期:2008-06-19 00:00:00

  • Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection.

    abstract:BACKGROUND:Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with em...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-240

    authors: França TG,Ishikawa LL,Zorzella-Pezavento SF,Chiuso-Minicucci F,Guerino CP,da Cunha Mde L,Sartori A

    更新日期:2009-11-23 00:00:00

  • AtlasT4SS: a curated database for type IV secretion systems.

    abstract:BACKGROUND:The type IV secretion system (T4SS) can be classified as a large family of macromolecule transporter systems, divided into three recognized sub-families, according to the well-known functions. The major sub-family is the conjugation system, which allows transfer of genetic material, such as a nucleoprotein, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-172

    authors: Souza RC,del Rosario Quispe Saji G,Costa MO,Netto DS,Lima NC,Klein CC,Vasconcelos AT,Nicolás MF

    更新日期:2012-08-09 00:00:00

  • Na+-stimulated phosphate uptake system in Synechocystis sp. PCC 6803 with Pst1 as a main transporter.

    abstract:BACKGROUND:Most living cells uptake phosphate, an indispensable nutrient for growth from their natural environment. In Synechocystis sp. PCC 6803, the cells lack phosphate-inorganic transport (Pit) system but contain two phosphate-specific transport (Pst) systems, Pst1 and Pst2. We investigated the kinetics of Pi uptak...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-225

    authors: Burut-Archanai S,Eaton-Rye JJ,Incharoensakdi A

    更新日期:2011-10-11 00:00:00

  • Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates.

    abstract:BACKGROUND:We and others have previously shown that alterations in the mammalian gut microbiome are associated with diet, notably early life exposure to a maternal high fat diet (HFD). Here, we aimed to further these studies by examining alterations in the gut microbiome of juvenile Japanese macaques (Macaca fuscata) t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1169-9

    authors: Pace RM,Prince AL,Ma J,Belfort BDW,Harvey AS,Hu M,Baquero K,Blundell P,Takahashi D,Dean T,Kievit P,Sullivan EL,Friedman JE,Grove K,Aagaard KM

    更新日期:2018-04-05 00:00:00

  • Allele distribution and genetic diversity of VNTR loci in Salmonella enterica serotype Enteritidis isolates from different sources.

    abstract:BACKGROUND:Salmonella enterica serotype Enteritidis (S. Enteritidis) is a zoonotic pathogen, which can be found in many sources including animals and the environment. However, little is known about the molecular relatedness among S. Enteritidis isolates from different sources. We have applied multiple-locus variable nu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-146

    authors: Cho S,Whittam TS,Boxrud DJ,Bartkus JM,Saeed AM

    更新日期:2008-09-15 00:00:00

  • Antagonistic interactions peak at intermediate genetic distance in clinical and laboratory strains of Pseudomonas aeruginosa.

    abstract:BACKGROUND:Bacteria excrete costly toxins to defend their ecological niche. The evolution of such antagonistic interactions between individuals is expected to depend on both the social environment and the strength of resource competition. Antagonism is expected to be weak among highly similar genotypes because most ind...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-40

    authors: Schoustra SE,Dench J,Dali R,Aaron SD,Kassen R

    更新日期:2012-03-22 00:00:00

  • Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS.

    abstract:BACKGROUND:The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desor...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-267

    authors: Lista F,Reubsaet FA,De Santis R,Parchen RR,de Jong AL,Kieboom J,van der Laaken AL,Voskamp-Visser IA,Fillo S,Jansen HJ,Van der Plas J,Paauw A

    更新日期:2011-12-23 00:00:00

  • The RNA interference pathway affects midgut infection- and escape barriers for Sindbis virus in Aedes aegypti.

    abstract:BACKGROUND:The RNA interference (RNAi) pathway acts as an innate antiviral immune response in Aedes aegypti, modulating arbovirus infection of mosquitoes. Sindbis virus (SINV; family: Togaviridae, genus: Alphavirus) is an arbovirus that infects Ae. aegypti in the laboratory. SINV strain TR339 encounters a midgut escape...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-130

    authors: Khoo CC,Piper J,Sanchez-Vargas I,Olson KE,Franz AW

    更新日期:2010-04-28 00:00:00

  • RpfC regulates the expression of the key regulator hrpX of the hrp/T3SS system in Xanthomonas campestris pv. campestris.

    abstract:BACKGROUND:The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. campestris recruits the hrp/T3SS system to inject pathogenicity effector proteins into host cells and uses the rpf/DSF cell-cell signaling system to regulate the expression of virulence factors such as extracellular enzymes and polysaccha...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1233-5

    authors: Jiang BL,Jiang GF,Liu W,Yang LC,Yang LY,Wang L,Hang XH,Tang JL

    更新日期:2018-09-03 00:00:00

  • Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

    abstract:BACKGROUND:The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-63

    authors: Sachse K,Laroucau K,Hotzel H,Schubert E,Ehricht R,Slickers P

    更新日期:2008-04-17 00:00:00

  • Salmonella induces prominent gene expression in the rat colon.

    abstract:BACKGROUND:Salmonella enteritidis is suggested to translocate in the small intestine. In vivo it induces gene expression changes in the ileal mucosa and Peyer's patches. Stimulation of Salmonella translocation by dietary prebiotics fermented in colon suggests involvement of the colon as well. However, effects of Salmon...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-84

    authors: Rodenburg W,Keijer J,Kramer E,Roosing S,Vink C,Katan MB,van der Meer R,Bovee-Oudenhoven IM

    更新日期:2007-09-12 00:00:00

  • The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803.

    abstract:BACKGROUND:The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS:We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phen...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-5-47

    authors: Morrison SS,Mullineaux CW,Ashby MK

    更新日期:2005-08-02 00:00:00

  • msaABCR operon is involved in persister cell formation in Staphylococcus aureus.

    abstract:BACKGROUND:Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1129-9

    authors: Sahukhal GS,Pandey S,Elasri MO

    更新日期:2017-11-22 00:00:00

  • The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age.

    abstract:BACKGROUND:In humans, the intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount the limitations of classical culturing techniques in order to obtain an accurate description...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-123

    authors: Mariat D,Firmesse O,Levenez F,Guimarăes V,Sokol H,Doré J,Corthier G,Furet JP

    更新日期:2009-06-09 00:00:00

  • Comparative gene expression profiling in two congenic mouse strains following Bordetella pertussis infection.

    abstract:BACKGROUND:Susceptibility to Bordetella pertussis infection varies widely. These differences can partly be explained by genetic host factors. HcB-28 mice are more resistant to B. pertussis infection than C3H mice, which could partially be ascribed to the B. pertussis susceptibility locus-1 (Bps1) on chromosome 12. The ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-88

    authors: Banus S,Vandebriel RJ,Pennings JL,Gremmer ER,Wester PW,van Kranen HJ,Breit TM,Demant P,Mooi FR,Hoebee B,Kimman TG

    更新日期:2007-10-12 00:00:00

  • Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.

    abstract:BACKGROUND:Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1423-9

    authors: Sánchez MC,Romero-Lastra P,Ribeiro-Vidal H,Llama-Palacios A,Figuero E,Herrera D,Sanz M

    更新日期:2019-03-12 00:00:00

  • Laboratory contamination in airway microbiome studies.

    abstract:BACKGROUND:The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have examined the impact of laboratory contamination on samples collected from the lower airways by pro...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1560-1

    authors: Drengenes C,Wiker HG,Kalananthan T,Nordeide E,Eagan TML,Nielsen R

    更新日期:2019-08-14 00:00:00

  • In vitro synergy of sertraline and tetracycline cannot be reproduced in pigs orally challenged with a tetracycline resistant Escherichia coli.

    abstract:BACKGROUND:Antimicrobial helper-compounds may reverse antimicrobial resistance. Sertraline, a antidepressant drug, has been suggested as a tetracycline helper-compound. Tetracycline is the preferred antimicrobial for treatment of enteric diseases in pigs. This study is the first to evaluate the potency of sertraline as...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1383-5

    authors: Kromann S,Hvidtfeldt A,Boye M,Sørensen DB,Jørgensen S,Nielsen JP,Olsen RH

    更新日期:2019-01-11 00:00:00

  • Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa.

    abstract:BACKGROUND:Leptospires lack many of the homologs for oxidative defense present in other bacteria, but do encode homologs of the Bacteriodes aerotolerance (Bat) proteins, which have been proposed to fulfill this function. Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific functi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-290

    authors: Stewart PE,Carroll JA,Dorward DW,Stone HH,Sarkar A,Picardeau M,Rosa PA

    更新日期:2012-12-13 00:00:00

  • Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A.

    abstract:BACKGROUND:Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) fol...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-97

    authors: Florio AR,Ferrari S,De Carolis E,Torelli R,Fadda G,Sanguinetti M,Sanglard D,Posteraro B

    更新日期:2011-05-11 00:00:00

  • Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33.

    abstract:BACKGROUND:The infections caused by antibiotic multidrug-resistant bacteria seriously threaten human health. To prevent and cure the infections caused by multidrug-resistant bacteria, new antimicrobial agents are required. Antimicrobial peptides are ideal therapy candidates for antibiotic-resistant pathogens. However, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0389-5

    authors: Mao R,Teng D,Wang X,Zhang Y,Jiao J,Cao X,Wang J

    更新日期:2015-03-03 00:00:00

  • Genetic diversity of Listeria monocytogenes strains in ruminant abortion and rhombencephalitis cases in comparison with the natural environment.

    abstract:BACKGROUND:Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both animals and humans. Here, multilocus sequence typing (MLST) was used to characterize the genetic diversity of Listeria monocytogenes strains isolated from the natural environment and animal clinical cases in Europe...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1676-3

    authors: Papić B,Pate M,Félix B,Kušar D

    更新日期:2019-12-18 00:00:00