当前位置： SCI文献检索 > EUROPEAN JOURNAL OF CLINICAL INVESTIGATION期刊下所有文献 > Pancreas transcription factor 1alpha expression is regulated in pancreatitis.

Pancreas transcription factor 1alpha expression is regulated in pancreatitis.

Abstract：

BACKGROUND:Expression of acinar cell-specific genes requires the pancreas transcription factor 1alpha (Ptf1alpha). p48 is the only component of Ptf1alpha that is involved in both acinar gene regulation and pancreatic ontogenesis. MATERIALS AND METHODS:To determine whether Ptf1alpha/p48 expression is regulated during pancreatitis, acute pancreatitis was induced in rats by repeated caerulein injections; early chronic pancreatitis by the combined administration of caerulein and cyclosporin A; and focal pancreas fibrosis by trinitrobenzene sulfonic acid infusion into the pancreatic duct. AR42J cells were used to examine caerulein effects on acinar cells. Ptf1alpha/p48 expression was examined using immunohistochemistry, Western blotting, and qRT-PCR methods. RESULTS:In acute pancreatitis, Ptf1alpha/p48 decreased markedly within 6 h as determined by Western blotting and immunohistochemistry. After 24 h, Ptf1alpha/p48 increased continuously and normalized at day six. In contrast, pancreas amylase reached a nadir at 48 h, when Ptf1alpha/p48 had largely recovered. In the early chronic pancreatitis model Ptf1alpha/p48 levels did not completely recover even at day 14, and this was associated with a failure to restore normal histology and amylase content. qRT-PCR showed that p48 mRNA were reduced after pancreatitis induction and were followed by a decrease in elastase mRNA. In the focal pancreas fibrosis model, Ptf1alpha/p48 expression was undetectable in areas with substantial acinar cell loss and tubular complexes. Caerulein did not affect Ptf1alpha/p48 expression in AR42J cells. CONCLUSIONS:Ptf1alpha/p48 protein and mRNA levels are regulated in acute and chronic experimental pancreatitis. Inability to re-express Ptf1alpha/p48 after injury may preclude acinar cell differentiation and appropriate pancreatic regeneration.

journal_name

Eur J Clin Invest

journal_title

European journal of clinical investigation

authors

Molero X,Adell T,Skoudy A,Padilla MA,Gómez JA,Chalaux E,Malagelada JR,Real FX

doi

10.1111/j.1365-2362.2007.01856.x

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

791-801

issue

eissn

0014-2972

issn

1365-2362

pii

ECI1856

journal_volume

pub_type

杂志文章

在线工具

Pancreas transcription factor 1alpha expression is regulated in pancreatitis.