Abstract:
:A continuous intravenous infusion of L-leucine (300 mumols min-1) was given to 12 healthy females over a 2 1/2 h period. Arterial plasma concentrations of amino acids and the keto acids of the branched-chain amino acids (BCAA) were measured. In six subjects muscle biopsies were taken before and at the end of the infusion for determination of intracellular (i.c.) free amino acid concentrations, and leg exchange of amino acids was measured. During infusion the plasma level of leucine rose sixfold. Approximately 40% of the infused amount was taken up by muscle. Of this, half was accumulated intracellularly, where the free leucine concentration increased from basal 190 +/- 22 to 580 +/- 110 mumols l-1 ICW (intracellular water) at the end of infusion. The concentrations of most other amino acids, above all the other BCAA and the aromatic amino acids, decreased, by 17-48% in the i.c. pool and by 17-79% in plasma. The plasma level of ketoisocaproic acid (KIC), the keto acid of leucine, increased in parallel with that of leucine. The concentration of keto valine, ketoisovaleric acid (KIV), decreased by 75%, whereas the keto acid of isoleucine, ketomethylvaleric acid (KMV), was unchanged. Leg release of alanine decreased significantly, whereas the exchange of other amino acids were unchanged. Taken together, decreased i.c. and plasma concentrations but unchanged leg exchange of tyrosine and phenylalanine suggest i.c. accumulation of protein. It can be calculated that approximately 40% of the leucine taken up by muscle was accumulated in the intracellular free pool, some 20% could have been incorporated into protein and 40% was probably oxidized.
journal_name
Eur J Clin Investjournal_title
European journal of clinical investigationauthors
Alvestrand A,Hagenfeldt L,Merli M,Oureshi A,Eriksson LSdoi
10.1111/j.1365-2362.1990.tb01858.xsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
293-8issue
3eissn
0014-2972issn
1365-2362journal_volume
20pub_type
杂志文章abstract:BACKGROUND:Obstructive sleep apnoea (OSA) is a common complication of obesity and can have a substantial negative impact on a patient's quality of life and risk of cardiovascular disease. The aim of this case-control study was to undertake discovery profiling of urinary peptides using capillary electrophoresis-mass spe...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12346
更新日期:2014-11-01 00:00:00
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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更新日期:2015-11-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:2002-02-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2011-06-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2000-12-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2006-09-01 00:00:00
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,多中心研究
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更新日期:1998-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-10-01 00:00:00
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更新日期:2021-01-01 00:00:00
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journal_title:European journal of clinical investigation
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doi:10.1111/j.1365-2362.2011.02643.x
更新日期:2012-07-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2018-09-01 00:00:00
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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更新日期:1992-10-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:1994-11-01 00:00:00
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更新日期:2013-03-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2003-12-01 00:00:00
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更新日期:2011-11-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2000-03-01 00:00:00
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更新日期:2015-07-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1996-07-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:2009-06-01 00:00:00