Abstract:
:The septins, which form a conserved family of cytoskeletal GTP-binding proteins in mammals, comprise stable heteromeric complexes and have diverse roles in protein scaffolding, cytokinesis, vesicle trafficking and plasma membrane integrity following cell division. The goal of this study was to determine the localization of septin 8 in murine adult retina, and analyze the spatiotemporal expression of septin 8 in a murine model of photoreceptor cell degeneration. Expression of septin 8 in the normal retina of mouse and rat was observed by using immunohistochemistry and Western blotting. Furthermore, time course of the expression of septin 8 in mouse photoreceptor cell degeneration were examined by immunohistochemistry combined with hematoxylin and eosin staining, and in situ DNA fragment labeling method. In normal mouse and rat retina, localization of septin 8 is restricted in nuclei of photoreceptor cells. 96 h after intravitreal injection of cobalt chloride most photoreceptor cells lost septin 8 immunostaining at the same time as nuclear DNA fragmentation. The results of this study show that septin 8 protein is present in the specific location within the retina. Furthermore, the disappearance of septin 8 in the nuclei of photoreceptor cells is concomitant with nuclear DNA fragmentation. This suggests that loss of septin 8 could be a useful prognostic indicator for photoreceptor cell degeneration.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Hara A,Taguchi A,Niwa M,Aoki H,Yamada Y,Ito H,Nagata K,Kunisada T,Mori Hdoi
10.1016/j.neulet.2007.07.030subject
Has Abstractpub_date
2007-08-23 00:00:00pages
205-10issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(07)00767-7journal_volume
423pub_type
杂志文章abstract::Saclofen (the direct sulphonic analogue of baclofen) is a competitive antagonist of baclofen at GABAB receptors in guinea pig ileum and rat cortical slices (estimated pA2 = 5.3), at least twice as potent as 2-hydroxy-saclofen (pA2 = 5). A series of related sulphonic analogues also antagonised baclofen in the guinea pi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90824-0
更新日期:1989-12-15 00:00:00
abstract::Peptide histidine-isoleucine (PHI) immunoreactivity was located in amacrine-like cells in adult rat retina by use of immunohistochemical techniques. Immunoreactive somata were found in the proximal part of the inner nuclear layer. From these somata, processes could be followed into the inner plexiform layer. The termi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90444-7
更新日期:1987-08-31 00:00:00
abstract::The N-methyl-D-aspartate (NMDA) receptor-induced inhibition of muscarinic receptor-stimulated phosphoinositide turnover in guinea pig cerebral cortical slices was investigated to determine whether polyamines regulate the function of NMDA in this system. None of the polyamines tested produced significant stimulation of...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90605-s
更新日期:1991-10-14 00:00:00
abstract::Using in situ hybridization histochemistry, we have demonstrated that 3 alpha subunit mRNAs of the GABAA receptor are present in different cell populations of the bovine cerebellum. While the alpha 1 mRNA is the most abundant and is present in granule cells, Purkinje cells and stellate/basket cells, the alpha 2 mRNA a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90193-6
更新日期:1989-11-20 00:00:00
abstract::The goat milk contains conjugated linoleic acid (CLA), which can influence physical growth and brain development. This study investigated the impact of a diet containing goat milk fat (GMF) on physical parameters of gestating (G) and/or lactating (L) rat dams, and their progeny's physical growth, and anxiety behavior....
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.07.028
更新日期:2013-09-27 00:00:00
abstract::The effects of the serotonin agonist MK-212, on rat plasma ACTH were examined. MK-212 significantly increased plasma ACTH levels, and this effect was blocked by the 5-HT1C antagonists mesulergine and metergoline but not by spiperone, ketanserin, or (-)-pindolol. The results suggest that MK-212 activates the 5-HT1C rec...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90032-3
更新日期:1989-10-23 00:00:00
abstract::A study was carried out to determine at what stage of development the adult pattern of primary afferent projections to the substantia gelatinosa (SG) is first established. Horseradish peroxidase conjugated wheat-germ agglutinin (WGA-HRP) was applied to cut ends of sciatic nerves at mid-thigh level in adult and neonata...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90179-9
更新日期:1983-12-30 00:00:00
abstract::Penicillins are thought to be GABA receptor antagonists. In order to determine the affinities of various penicillin derivatives for the GABA receptor, their potencies as inhibitors of specific [3H]GABA binding to rat brain membranes were investigated. All investigated penicillins inhibit specific [3H]GABA binding, wit...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(80)90302-x
更新日期:1980-07-01 00:00:00
abstract::Localization of 5-hydroxytryptamine immunoreactivity was studied in the rat coeliac-superior mesenteric ganglion complex and in the porcine superior and inferior mesenteric ganglia by the indirect immunofluorescence technique. In normal rats, only 5-hydroxytryptamine immunoreactive SIF cells were seen in the coeliac-s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11733-d
更新日期:1995-07-14 00:00:00
abstract::Experimental findings have suggested the possibility of a functional relationship between the basal ganglia and the hippocampus. Previous research has revealed a predominantly inhibitory action of the caudate nucleus (CN) and an excitatory effect of the globus pallidus (GP) on electrically induced hippocampal afterdis...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90526-4
更新日期:1986-12-23 00:00:00
abstract::Alterations in dendritic spine densities and morphologies have been correlated with the abnormal functioning of the synapse. Specifically the metabotropic glutamate receptor 5 (mGluR5) has been implicated in dendrogenesis and spineogenesis, since its activation triggers various signaling cascades that have been demons...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.07.014
更新日期:2012-08-22 00:00:00
abstract::gamma-Aminobutyric acid (GABA)-mediated neurotransmission from the granule cells to CA3 is transiently expressed during the first 3 weeks of age in the rat. In the adult, seizures provoke this inhibitory signaling to reappear. To gain insight into the origin of GABA in these cells, we explored the expression of both i...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.08.077
更新日期:2003-12-15 00:00:00
abstract::The role of hippocampal noradrenergic inputs in the modulation of corticosteroid receptor expression has been investigated. Adult male rats were given central 6-hydroxydopamine (6-OHDA) and the expression of hippocampal mineralocorticoid (MR) and glucocorticoid (GR) receptor mRNA was examined after two weeks by in sit...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90363-c
更新日期:1992-08-17 00:00:00
abstract::Cholinergic projections originating in the basal forebrain (BF) play important roles in the heterosynaptic facilitation of synaptic strength in various sensory cortices, including the primary visual cortex (V1). Here, using urethane-anesthetized rats, we find that pairing burst stimulation of the BF with single pulse ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.07.052
更新日期:2009-10-02 00:00:00
abstract::Neurofibromatosis 1 (NF1) is a common autosomal dominant disorder in which affected individuals develop benign and malignant tumors as well as non-tumor-related abnormalities, such as seizures and learning disabilities. Here, we report an NF1 isoform arising from the alternative splicing of exon 9a with predominant ce...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12730-0
更新日期:1996-06-21 00:00:00
abstract::The cerebral-buccal interneurons (CBIs) in Aplysia are a group of inter-ganglionic projection neurons that regulate feeding motor programs. In this study, electrophysiological and immunocytological methods were used to identify a previously uncharacterized CBI, designated CBI-11. CBI-11 is a gamma-aminobutyric acid (G...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00052-1
更新日期:2003-04-24 00:00:00
abstract::We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90819-2
更新日期:1994-12-05 00:00:00
abstract::Previously, trophic effects of extracts from whole chick embryos and from innervated muscles on cultured muscle cells were described. The present study demonstrated similar effects of extracts from 10-days denervated chick muscles. Extracts from innervated as well as from denervated muscles exsanguinated in vivo with ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90283-4
更新日期:1981-02-23 00:00:00
abstract::We examined the intracellular Ca2+ ([Ca2+]i) elevation evoked by GABA in an 8-day embryonic chick brainstem slice using a Ca imaging technique with Ca green-1 AM. When small quantities of GABA were pressure-ejected on the surface of the slice, the [Ca2+]i elevation was clearly detected. The GABA-induced [Ca2+]i elevat...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.10.019
更新日期:2007-01-03 00:00:00
abstract::Thyrotropin-releasing hormone (TRH) metabolism in NIH:N mouse cerebral cortex and spinal cord was studied with high pressure liquid chromatography. Degradation of added TRH was significantly (P less than 0.001) slower in the spinal cord (2.19 +/- 0.46%/min) than in the cerebral cortex (7.54 +/- 0.31%/min). Bacitracin,...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90560-4
更新日期:1986-11-11 00:00:00
abstract::Five monkeys vocalizing at self-pace (self-paced vocalization) were well trained to vocalize in response to a monkey call (audio-initiated vocalization). Field potentials associated with audio-initiated vocalizations were recorded by using electrodes which were implanted chronically on the surface and at a 2.0-3.0 mm ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00570-4
更新日期:1999-09-03 00:00:00
abstract::Nociceptin/orphanin FQ (NC) and its receptor (OP(4)) have been implicated in the regulation of various functions including nociception. [Nphe(1)]NC(1-13)NH(2) (Nphe) is a selective OP(4) antagonist which prevents the pronociceptive effects of supraspinal NC and causes per se a naloxone-insensitive antinociceptive effe...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02352-7
更新日期:2001-12-04 00:00:00
abstract::Traumatic stress has been believed to result in a variety of unusual alterations of the integrity and the functionality in the hippocampus. In this study, we searched for genes responsive to traumatic stress in the mouse hippocampus to elucidate the underlying mechanisms. Adult male mice were subjected to water-immers...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.12.062
更新日期:2008-03-15 00:00:00
abstract::Changes in the thiol/disulphide status in the neonatal rat brain were evaluated after an episode of neonatal hypoxia-ischemia (HI) in 7-day-old rats. The glutathione level decreased in the post-HI period. The lowest values (43-68%) were obtained 24 h post-HI. A statistically significant difference first appeared in hi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00510-x
更新日期:2003-07-10 00:00:00
abstract::In the adult, the dickkopf family member Dickkopf-like1 (Dkkl1) has been described as a testicular protein involved in the regulation of spermatocyte apoptosis. However, microarray studies additionally suggested that Dkkl1 regulation is involved in various tumors including high grade gliomas. Since investigations of D...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.12.040
更新日期:2013-02-22 00:00:00
abstract::The number of neurons in the primary motor cortex (MI) and the primary somatosensory cortex (SI) were estimated in the same locations of brains from sporadic amyotrophic lateral sclerosis (ALS) cases and controls. The number of MI and SI neurons and Betz cells were significantly decreased in the ALS cases as compared ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.08.014
更新日期:2011-09-26 00:00:00
abstract::The cellular localization of butyrylcholinesterase (E,C 3.1.1.8) in rat cerebellum was determined by immunofluorescence with a monospecific immune serum raised against rat brain butyrylcholinesterase. The antiserum specifically stains only glial cells. ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90068-x
更新日期:1983-08-29 00:00:00
abstract::Galanin- and bethanechol-activated whole cell currents were studied in dissociated mudpuppy parasympathetic neurons kept in 12.5 mM KCl. The bethanechol-induced current was reduced when generated during a galanin-induced current. The galanin and bethanechol currents reversed at -45 mV and exhibited inward rectificatio...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90675-w
更新日期:1992-06-08 00:00:00
abstract::Although dopaminergic neurons are thought to exist in the lateral olivocochlear efferent system and modulate the afferent nerve activity, the distribution of dopamine (DA) receptor subtypes is still obscure. In the present study, we investigated the localization of five subtypes of DA receptor (D1-5) by immunocytochem...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.01.063
更新日期:2006-05-22 00:00:00
abstract::Walking with different types of cueing/stimulus (i.e. auditory, visual) has been shown to alter gait variability, thus emerging as an innovative therapeutical tool to restore abnormal gait variability in clinical populations. However, the majority of the research in this area has focused on auditory stimuli while visu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2019.03.040
更新日期:2019-06-21 00:00:00