Abstract：
:Although synaptically released, vesicular Zn(2+) has been proposed to play a neuromodulatory or neuronal signaling role at the mossy fiber-CA3 synapse, Zn(2+) release remains controversial, especially when detected using fluorescent imaging. In the present study, we investigated synaptically released Zn(2+) at the mossy fiber (MF) synapse in rat hippocampal slices using three chemically distinct, fluorescent Zn(2+) indicators. The indicators employed for this study were cell membrane impermeable (or extracellular) Newport Green [K(DZn2+) approximatelly 1 microM] , Zinpyr-4 K(DZn2+) approximately 1 nM and FluoZin-3 K(DZn2+) approximately 15 nM, chosen, in part, for their distinct dissociation constants. Among the three indicators, FluoZin-3 was also sensitive to Ca(2+) K(DCa2+) approximately 200-300 microM which was present in the extracellular medium ([Ca(2+)](o)>2mM). Hippocampal slices loaded with either Newport Green or FluoZin-3 showed increases in fluorescence after electrical stimulation of the mossy fiber pathway. These results are consistent with previous studies suggesting the presence of synaptically released Zn(2+) in the extracellular space during neuronal activities; however, the rise in FluoZin-3 fluorescence observed was complicated by the data that the addition of exogenous Zn(2+) onto FluoZin-3 loaded slices gave little change in fluorescence. In the slices loaded with the high-affinity indicator Zinpyr-4, there was little change in fluorescence after mossy fiber activation by electrical stimulation. Further study revealed that the sensitivity of Zinpyr-4 was mitigated by saturation with Zn(2+) contamination from the slice. These data suggest that the sensitivity and selectivity of a probe may affect individual outcomes in a given experimental system.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Bastian C,Li YVdoi
10.1016/j.neulet.2007.04.047subject
Has Abstractpub_date
2007-05-29 00:00:00pages
119-24issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(07)00467-3journal_volume
419pub_type
杂志文章abstract：:Localized moderate cooling (+10 degrees C) of the anterior and ventral part of the raphe dorsalis induced cortical synchronization and ponto-geniculo-occipital (PGO) waves followed sometimes by paradoxical sleep. Cooling the region of the raphe magnus produced cortical arousal. After depletion of brain 5-hydroxytrypta...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(76)90077-x
更新日期：1976-11-01 00:00:00
abstract：:Recent neuroimaging studies indicate that there may be common ground for esthetic and moral judgments. However, because previous studies focused on either esthetic or moral judgments and did not compare the two directly, the issue remains open whether a common ground actually exists. We employed functional magnetic re...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.11.053
更新日期：2013-02-08 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90081-3
更新日期：1985-08-05 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.11.053
更新日期：2016-03-11 00:00:00
abstract：:The intramedullary axon collateral systems of adult cat spinal alpha-motoneurones were studied with morphological and physiological techniques 12 weeks after peripheral transection of the motor axons. Morphologically, intracellular horseradish peroxidase (HRP) staining showed that the axotomy induced an elimination of...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(84)90343-4
更新日期：1984-11-23 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00715-2
更新日期：2002-09-13 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.06.004
更新日期：2006-10-09 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)12198-6
更新日期：1995-12-29 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.10.012
更新日期：2007-12-18 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)00872-7
更新日期：2000-03-17 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.07.049
更新日期：2005-12-16 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(84)90040-5
更新日期：1984-02-24 00:00:00
abstract：:Recent discovery of pathogenic mutations in the leucine-rich repeat kinase 2 (LRRK2) gene in Parkinson's disease (PD) patients in different ethnic groups have raised a hope of diagnostic screening and genetic counseling. We investigated the six most commonly reported mutations in LRRK2 gene among Indian PD patients, u...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.04.052
更新日期：2006-12-01 00:00:00
abstract：:Application of strychnine sulfate (10-50 mu M) to the anterior hindlimb enlargement of the turtle spinal cord increased the amplitude of the ipsilateral fictive flexion reflex and revealed a contralateral (crossed) fictive flexion reflex response to cutaneous stimulation of the foot. Strychnine abolished the crossed i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12387-9
更新日期：1996-02-23 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.03.010
更新日期：2009-05-15 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.08.061
更新日期：2004-11-23 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135337
更新日期：2020-11-01 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11681-l
更新日期：1995-06-30 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90852-4
更新日期：1994-01-17 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.11.047
更新日期：2013-02-08 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.07.088
更新日期：2008-10-10 00:00:00
abstract：:DNA variation at the FGF20 gene has been associated with Parkinson's disease (PD). In particular, SNP rs12720208 in the 3' untranslated region (3' UTR) was linked to PD-risk through a mechanism that would implicate a differential binding to microRNA-433 (miR-433). The reduction of the affinity of miR-433 to the 3' UTR...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.05.019
更新日期：2010-07-19 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(76)90066-5
更新日期：1976-12-01 00:00:00
abstract：:Activation of serotonin (5-hydroxytryptamine, 5-HT) receptors produces various autonomic and neuroendocrine responses in the hypothalamic paraventricular nucleus (PVN), including increased blood pressure and heart rate. However, the role(s) of 5-HT on the local GABA synaptic circuit have not been well understood in th...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.05.012
更新日期：2008-07-11 00:00:00
abstract：:A new method for on-line analysis of in vivo differential normal pulse voltammograms is reported. They are fitted by least squares to an expression describing the contribution of different electroactive species and the baseline. Its validity for resolving ascorbate (AA), dopamine (DA) and DOPAC signals is evidenced by...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90183-8
更新日期：1988-03-21 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90655-5
更新日期：1993-07-09 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.09.043
更新日期：2014-11-07 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135548
更新日期：2021-01-18 00:00:00
abstract：:Nitric oxide synthase-immunoreactivity (NOS-IR) was detected in strands of nerve fibers entering the rat superior cervical ganglia (SCG) and in nerve fibers forming a plexus beneath the capsule of adrenal glands. Within the SCG, varicose NOS-IR fibers encircled virtually all postganglionic neurons and small diameter c...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90610-w
更新日期：1993-08-06 00:00:00
abstract：:Chemical irritation of the urinary bladder with formalin in the rat induced c-fos protein-like immunoreactivity in more than 80% of substance P receptor-like immunoreactive (SPR-LI) neurons of the dorsal commissural nucleus, sacral parasympathetic nucleus and lamina I in the 6th lumbar and 1st sacral cord segments. Th...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11991-5
更新日期：1995-09-29 00:00:00
