Abstract：
:A surface functionalization strategy that produces substrates with well-controlled ligand density is critical to investigating the role of cell-substrate interactions in regulating cell adhesion, viability, migration, proliferation and differentiation. Towards this end, we have designed and synthesized a triblock protein, CRC, comprising a polyelectrolyte domain flanked by two amphiphilic leucine zipper domains. The amphiphilic end domains of CRC adsorb onto surfaces and preferentially associate into trimeric aggregates, forming a hydrogel coating layer. Under serum-free conditions, the CRC coating was found to render both 2D substrates and 3D scaffolds non-adhesive to cells. A RGDS sequence was inserted in the middle domain of CRC (generating the protein CRC-RGDS) and found to introduce cell-binding activity. Incorporation of the RGDS sequence did not significantly impact the surface activity of CRC, allowing us to titrate the RGDS surface density simply by adjusting the relative ratios of the two proteins. Ligand density dependent cell-substrate interactions were demonstrated in human foreskin fibroblasts, human umbilical vein endothelial cells, and rat neural stem cells. The versatility to functionalize a range of different substrate surfaces, combined with the ease of controlling surface ligand density, makes these triblock proteins an attractive tool for developing cell-specific surface coatings with tailored biofunctional attributes.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Fischer SE,Liu X,Mao HQ,Harden JLdoi
10.1016/j.biomaterials.2007.03.026subject
Has Abstractpub_date
2007-08-01 00:00:00pages
3325-37issue
22eissn
0142-9612issn
1878-5905pii
S0142-9612(07)00262-1journal_volume
28pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:The shelf life of implantable materials has rarely been addressed. We determined whether osteoconductivity of titanium is stable over time. Rat bone marrow-derived osteoblasts were cultured on new titanium disks (immediately after acid-etching), 3-day-old (stored after acid-etching for 3 days in dark ambient condition...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.06.040
更新日期：2009-10-01 00:00:00
abstract：:Helicobacter pylori (H. pylori) infection is highly prevalent, and has a strong association with various gastric diseases, including gastritis, digestive ulcers, and cancer. H. pylori strains with resistance to existing antibiotics have emerged in the past two decades. Currently, treatment of H. pylori infection (invo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.05.014
更新日期：2015-08-01 00:00:00
abstract：:Remarkable water-stimulated shape memory effect was revealed in a natural biopolymer of peacock's tail covert feathers of which the innate shape can almost be fully recovered after severe deformation by a short hydration step. The shape memory effect manifests a good stability of high recovery rate and ratio during cy...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.06.032
更新日期：2015-10-01 00:00:00
abstract：:Combination therapy is usually considered as a promising strategy owing to its advantages such as reduced doses, minimized side effects and improved therapeutic efficiency in a variety of diseases including diabetes. Here we synthesized a new highly intracellular stimuli-sensitive chitosan-graft-metformin (CS-MET) pro...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.09.013
更新日期：2015-12-01 00:00:00
abstract：:We design a class of imprinted hybrid microgels that can optically monitor glucose levels with high sensitivity and selectivity in complex media at physiological pH, acting like a "glucose-indicator". Such imprinted hybrid microgels were made of Ag nanoparticles (NPs) in situ immobilized in molecularly imprinted gluco...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.06.031
更新日期：2012-10-01 00:00:00
abstract：:In this work, analytical transmission electron microscopy (TEM) was used to study the nanostructure of mineralised ivory dentine, in order to gain a clearer understanding of the relationship between the organic (collagen fibrils) and inorganic (calcium phosphate apatite crystals) components. Thin sections prepared by ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.03.025
更新日期：2010-07-01 00:00:00
abstract：:Tissue engineering requires the use of polymeric scaffolds that mimic many roles of extracellular matrices (ECM) in the body. Controlling cell-scaffold interactions is one of the most critical parameters for regulating cell phenotype in tissue engineering, and a peptide with the sequence of RGD has been widely exploit...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.03.063
更新日期：2010-07-01 00:00:00
abstract：:Alginate-polylysine (PLL) capsules are commonly applied for immunoprotection of endocrine tissues. Alginate is composed of mannuronic acid (M) and guluronic acid (G). Different types of alginate have different ratios of G to M, but little is known of the influence of these differences on biocompatibility. Therefore, w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(96)00135-4
更新日期：1997-02-01 00:00:00
abstract：:Literature has shown that slowing down the curing rate of light-initiated resin composites by lowering the light intensity of the light source improved the integrity of the adhesive composite-cavity interface as a result of a more gradual contraction stress development. This study investigated how the curing rate coul...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(96)00074-9
更新日期：1996-12-01 00:00:00
abstract：:We developed cell-free implants, comprising carbodiimide crosslinked recombinant human collagen (RHC), to enable corneal regeneration by endogenous cell recruitment, to address the worldwide shortage of donor corneas. Patients were grafted with RHC implants. Over four years, the regenerated neo-corneas were stably int...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.11.079
更新日期：2014-03-01 00:00:00
abstract：:Nanomedicine options for colon cancer therapy have been limited by the lack of suitable carriers capable of delivering sufficient drug into tumors to cause lethal toxicity. To circumvent this limitation, we fabricated a camptothecin (CPT)-loaded poly(lactic-co-glycolic acid) nanoparticle (NP) with dual-surface functio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.01.014
更新日期：2015-04-01 00:00:00
abstract：:A newly developed bone equivalent hydroxyapatite was derived from veterinary bone (VHAP). Sections of 1 cm of six rabbit mandibles were equally replaced by this VHAP graft. Radiological studies by X-ray were performed pre-operatively, immediately, and 1, 2 and 3 months post-operatively. The graft host-bone interface w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(94)90211-9
更新日期：1994-06-01 00:00:00
abstract：:Cell movement on adhesive surfaces is a complicated process based on myriad cell-surface interactions. Although both micron and nanoscale surface topography have been known to be important in understanding cell-materials interactions, typically only simple patterns (e.g., parallel lines or aligned posts) have been use...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.01.103
更新日期：2010-05-01 00:00:00
abstract：:Implantation of a medical implant within the body inevitably triggers a host inflammatory response that negatively impacts its function and longevity. Nevertheless, the degree and severity of this response may be reduced by selecting appropriate materials, implant geometry, surface topography and surface treatment. He...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.01.005
更新日期：2015-04-01 00:00:00
abstract：:Nitric oxide (NO) is an important biological messenger involved in the treatment of bacterial infections, but its controlled and targeted release in bacterial infections remains a major challenge. Herein, an intelligent NO nanogenerator triggered by near-infrared (NIR) light is constructed for targeted treatment of P....
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120588
更新日期：2021-01-01 00:00:00
abstract：:Targeted drug delivery with precisely controlled drug release and activation is highly demanding and challenging for tumor precision therapy. Herein, a biomimetic cascade nanoreactor (designated as Mem@GOx@ZIF-8@BDOX) is constructed for tumor targeted starvation therapy-amplified chemotherapy by assembling tumor cell ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.01.003
更新日期：2019-03-01 00:00:00
abstract：:Tissue engineering strategies for repairing and regenerating articular cartilage face critical challenges to recapitulate the dynamic and complex biochemical microenvironment of native tissues. One approach to mimic the biochemical complexity of articular cartilage is through the use of recombinant bacterial collagens...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.05.011
更新日期：2016-08-01 00:00:00
abstract：:The interactions between alginate and polycations have been studied by using different labelling techniques. Binding of poly-L-lysine (PLL) to alginate in the gel state is mainly governed by the amount of dissociable negative charges on the bead surface. PLL was found to bind more rapidly to gel beads made from algina...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)84680-1
更新日期：1996-05-01 00:00:00
abstract：:To maintain enzymatic activity during long-term storage by conjugation with water-soluble 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers (PMPC-COOH) having various molecular weights with a carboxylic group on the terminal, such compounds were synthesized as a polymer modifier using a photoinduced living radic...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00474-5
更新日期：2004-01-01 00:00:00
abstract：:Glucose oxidase was immobilized into photographic gelatin hardened by chromium(III) sulphate. The enzyme-gelatin mixture was coated on polyester film strips which allowed easy and simple handling during assays. The effect of gelatin and cross-linker concentrations on water content and enzymatic activity was studied. T...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(92)90064-u
更新日期：1992-01-01 00:00:00
abstract：:Understanding mechanisms that govern cell fate determination of human induced pluripotent stem cells (hiPSCs) could assist in maintenance of the undifferentiated state during cell expansion. We used polyamidoamine dendrimer surfaces with first-generation (G1), third-generation (G3) and fifth-generation (G5) of dendron...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.03.085
更新日期：2014-07-01 00:00:00
abstract：:The production profile of interleukin 1 (IL-1) from mouse peritoneal macrophages (M phi) was determined following their incubation with poly(DL-lactic acid) (PDLLA) granules containing ovalbumin (OVA). Upon incubation, M phi produced IL-1 at a significantly high rate compared with those incubated with OVA in the free ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)00034-8
更新日期：1996-12-01 00:00:00
abstract：:Following our recent report of attractive antibacterial properties of a designed amphiphilic peptide, A(9)K, we have investigated its antitumor activities by examining the modes of its action against different mammalian cell types. The peptide strongly inhibited the growth of cancerous HeLa cells and human promyelocyt...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.12.039
更新日期：2013-04-01 00:00:00
abstract：:Palladium additions to a dispersed phase high-copper amalgam have been shown recently to suppress markedly the eta' (Cu6Sn5) concentration and decrease creep. A detailed study of the dental and 24 h creep for Pd containing high-copper amalgams and six commercial controls as a function of applied temperature and stress...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(88)90086-5
更新日期：1988-05-01 00:00:00
abstract：:Polymers may serve as a biodegradable material in tissue engineering. To assess the biocompatibility of poly-beta-hydroxy butyric acid (PHB), we studied the structural organization of cellular molecules involved in adhesion using osteoblastic and epithelial cell lines. On PHB, both cell lines revealed a rounded cell s...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00430-0
更新日期：2001-09-01 00:00:00
abstract：:TiO(2)-coated biomaterials that have been excited with UV irradiation have demonstrated biocidal properties in environmental applications, including drinking water decontamination. However, this procedure has not been successfully applied towards the killing of pathogens on medical titanium-based implants, mainly beca...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.03.005
更新日期：2010-07-01 00:00:00
abstract：:Human cell therapy applications in tissue engineering, such as the ex vivo production of hematopoietic cells for transplantation, have recently entered the clinic. Although considerable effort has been focused on the development of biological processes to generate therapeutic cells, little has been published on the de...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00101-x
更新日期：1998-11-01 00:00:00
abstract：:Heat treatment of an MgO-CaO-SiO2-P2O5 glass gave a glass ceramic containing crystalline apatite (Ca10(PO4)6O,F2] and beta-wollastonite (CaO,SiO2) in an MgO-CaO-SiO2 glassy matrix. It showed bioactivity and a fairly high mechanical strength which decreased only slowly, even under load-bearing conditions in the body. I...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(91)90194-f
更新日期：1991-03-01 00:00:00
abstract：:The effect of hirudin, a known inhibitor of thrombin, was evaluated for whole blood samples in terms of platelet deposition/adhesion to a non-biological test surface (tetrafluoroethylene-propylene copolymer), adenosine diphosphate (ADP) release and reduction in platelet count during laminar shear flow for a shear rate...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(93)90009-q
更新日期：1993-09-01 00:00:00
abstract：:The FRP wires 0.5 mm in diameter with a multiple fiber structure were fabricated by drawing the fiber polymer complex at 250 degrees C for an esthetic, transparent orthodontic wire. Biocompatible CaO-P2O5-SiO2-Al2O3 (CPSA) glass fibers of 8-20 microm in diameter were oriented unidirectionally in the longitudinal direc...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00127-6
更新日期：1998-12-01 00:00:00
