Isogenic Strains of PHYCOMYCES BLAKESLEEANUS Suitable for Genetic Analysis.

Abstract:

:The progeny of crosses between wild-type strains of Phycomyces usually do not exhibit all of the expected genotypes from meiosis. By backcrossing, we have isolated a new (+) mating-type strain, A56, which is nearly isogenic with the (-) wild-type NRRL1555 commonly used in Phycomyces research. Tetrad analysis of the backcrosses shows that meiosis becomes more regular as the parental (+) and (-) strains become more isogenic. In our two-factor crosses with unlinked markers, the regularity of meiosis is measured as the percent of reciprocal ditypes plus tetratypes in the progeny. We have shown that this percentage increases from about 15% for crosses between nonisogenic parents to 90% in the eighth backcross. The results indicate that routine, reliable recombination analyses are possible in P. blakesleeanus.

journal_name

Genetics

journal_title

Genetics

authors

Alvarez MI,Eslava AP

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

873-9

issue

4

eissn

0016-6731

issn

1943-2631

journal_volume

105

pub_type

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