Abstract：
:Motile and immotile (or primary) cilia are microtubule-based structures that mediate multiple cellular functions, including the transduction of environmental cues, developmental signaling, cellular motility, and modulation of fluid flow. Although their core architectures are similar, motile and primary cilia exhibit marked structural differences that underlie distinct functional properties. However, the extent to which ciliogenesis mechanisms are shared between these different cilia types is not fully described. Here, we report that the atypical MAP kinase MAPK15 (ERK7/8), implicated in the formation of vertebrate motile cilia, also regulates the formation of primary cilia in Caenorhabditis elegans sensory neurons and human cells. We find that MAPK15 localizes to a basal body subdomain with the ciliopathy protein BBS7 and to cell-cell junctions. MAPK15 also regulates the localization of ciliary proteins involved in cilium structure, transport, and signaling. Our results describe a primary cilia-related role for this poorly studied member of the MAPK family in vivo, and indicate a broad requirement for MAPK15 in the formation of multiple ciliary classes across species.
journal_name
Geneticsjournal_title
Geneticsauthors
Kazatskaya A,Kuhns S,Lambacher NJ,Kennedy JE,Brear AG,McManus GJ,Sengupta P,Blacque OEdoi
10.1534/genetics.117.300383subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1423-1440issue
4eissn
0016-6731issn
1943-2631pii
genetics.117.300383journal_volume
207pub_type
杂志文章相关文献
GENETICS文献大全abstract：:In order to examine the mechanisms of mutagenesis by a bulky DNA lesion at the guanine N7 position, the replicative form DNA of phage M13AB28 (mp8 without the amber codons in phage genes) was modified in vitro with aflatoxin B1-2,3-dichloride and transfected into appropriate Escherichia coli cells. Forward mutations i...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Advances in medicine, agriculture, and an understanding of evolution depend on resolving the genetic architecture of quantitative traits, which is challenging since variation for complex traits is caused by multiple interacting quantitative trait loci (QTL) with small and conditional effects. Here, we show that the ke...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:The primary signal for Drosophila sex determination is the number of X chromosomes relative to the number of sets of autosomes. The present report shows that the numerator of this X/A signal appears to be determined by the cumulative dose of a relatively limited number of discrete X-linked genetic elements, two of whi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:The Sex-Ratio (SR) phenomenon is associated with the X chromosome of many species of Drosophila. Males carrying SR transmit predominantly sperm bearing the X chromosome. SR, therefore, has a very significant advantage in segregation. This paper provides an experimental analysis of the role of virility selection in pre...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:No proper statistical test is available for the evaluation of deviation of a single homozygous genotype from Hardy-Weinberg equilibrium (HWE) proportion. We propose a 1-d.f. chi2-test. The power of the proposed test is favorable compared to existing HWE testing procedures. The applications of this test are discussed. ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.043190
更新日期：2005-07-01 00:00:00
abstract：:The application of quantitative genetics in plant and animal breeding has largely focused on additive models, which may also capture dominance and epistatic effects. Partitioning genetic variance into its additive and nonadditive components using pedigree-based models (P-genomic best linear unbiased predictor) (P-BLUP...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.171322
更新日期：2014-12-01 00:00:00
abstract：:DnaB is the helicase associated with the DNA polymerase III replication fork in Escherichia coli. Previously we observed that the dnaB107(ts) mutation, at its permissive temperature, greatly stimulated deletion events at chromosomal tandem repeats. This stimulation required recA, which suggests a recombinational mecha...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:We previously identified a transcriptional regulatory element, which we call NRE(DIT), that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae. Repression through this element is dependent on the Ssn6-Tup1 corepressor. In this study, we sho...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Many genes that affect axon pathfinding and cell migration have been identified. Mechanisms by which these genes and the molecules they encode interact with one another in pathways and networks to control developmental events are unclear. Rac GTPases, the cytoskeletal signaling molecule Enabled, and NIK kinase have al...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.046359
更新日期：2006-02-01 00:00:00
abstract：:We found that heterozygous mutant alleles of E(Pc) and esc increased homologous recombination from an allelic template in somatic cells in a P-element-induced double-strand break repair assay. Flies heterozygous for mutant alleles of these genes showed increased genome stability and decreased levels of apoptosis in im...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.042473
更新日期：2006-04-01 00:00:00
abstract：:The parentals of the interspecific cross Cucurbita maxima xC. ecuadorensis had different isozyme phenotypes for 12 enzyme systems. Characterization of the systems demonstrated that the expression and intracellular distribution of the isozymes were similar to those in other plant taxa; however, a considerable number of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The relationships between synonymous and nonsynonymous substitution rates and between synonymous rate and codon usage bias are important to our understanding of the roles of mutation and selection in the evolution of Drosophila genes. Previous studies used approximate estimation methods that ignore codon bias. In this...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The regulation of chemoreceptor (CR) gene expression by environmental signals and internal cues may contribute to the modulation of multiple physiological processes and behavior in Caenorhabditis elegans. We previously showed that KIN-29, a homolog of salt-inducible kinase, acts in sensory neurons to regulate the expr...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.094771
更新日期：2008-11-01 00:00:00
abstract：:Candida albicans, which is diploid, possesses a single mating-type (MTL) locus on chromosome 5, which is normally heterozygous (a/alpha). To mate, C. albicans must undergo MTL homozygosis to a/a or alpha/alpha. Three possible mechanisms may be used in this process, mitotic recombination, gene conversion, or loss of on...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.033167
更新日期：2005-03-01 00:00:00
abstract：:Prions have revived interest in hereditary change that is due to change in cellular structure. How pervasive is structural inheritance and what are its mechanisms? Described here is the initial characterization of [Leu(P)], a heritable structural change of the mitochondrion of Saccharomyces cerevisiae that often but n...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
abstract：:We have investigated meiotic changes in CAG repeat tracts embedded in a yeast chromosome. Repeat tracts undergo either conversion events between homologs or expansion and contraction events that appear to be confined to a single chromatid. We did not find evidence for conversion of tract interruptions or excess exchan...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Great interest was aroused by reports, based on microsatellite markers, of high levels of statistically significant long-range and nonsyntenic linkage disequilibrium (LD) in livestock. Simulation studies showed that this could result from population family structure. In contrast, recent SNP-based studies of livestock ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.097402
更新日期：2009-02-01 00:00:00
abstract：:An algorithm for detecting local clusters of homologous genes was applied to the genome of Caenorhabditis elegans. Clusters of two or more homologous genes are abundant, totaling 1391 clusters containing 4607 genes, over one-fifth of all genes in C. elegans. Cluster genes are distributed unevenly in the genome, with t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.040030
更新日期：2006-01-01 00:00:00
abstract：:Segregation distorter (SD) chromosomes are preferentially transmitted to offspring from heterozygous SD/SD(+) males owing to the induced dysfunction of the SD(+)-bearing sperm. This phenomenon involves at least two major loci: the Sd locus whose presence is necessary for distortion to occur and the Rsp locus which act...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：:Dna2 is a nuclease and helicase that functions redundantly with other proteins in Okazaki fragment processing, double-strand break resection, and checkpoint kinase activation. Dna2 is an essential enzyme, required for yeast and mammalian cell viability. Here, we report that numerous mutations affecting the DNA damage ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300809
更新日期：2018-05-01 00:00:00
abstract：:One of the most important parameters in population genetics is theta = 4Ne mu where Ne is the effective population size and mu is the rate of mutation per gene per generation. We study two related problems, using the maximum likelihood method and the theory of coalescence. One problem is the potential improvement of a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Mitochondrial complex I is the largest multimeric enzyme of the respiratory chain. The lack of a model system with facile genetics has limited the molecular dissection of complex I assembly. Using Chlamydomonas reinhardtii as an experimental system to screen for complex I defects, we isolated, via forward genetics, am...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.128827
更新日期：2011-06-01 00:00:00
abstract：:Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303419
更新日期：2020-09-01 00:00:00
abstract：:The protein phosphatase calcineurin is central to Ca2+ signaling pathways from yeast to humans. Full activation of calcineurin requires Ca2+ binding to the regulatory subunit CNB, comprised of four Ca2+-binding EF hand domains, and recruitment of Ca2+-calmodulin. Here we report the consequences of disrupting Ca2+ bind...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300911
更新日期：2018-07-01 00:00:00
abstract：:Conspicuous differences in floral morphology are partly responsible for reproductive isolation between two sympatric species of monkeyflower because of their effect on visitation of the flowers by different pollinators. Mimulus lewisii flowers are visited primarily by bumblebees, whereas M. cardinalis flowers are visi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:It is well known that an allele causing increased recombination is expected to proliferate as a result of genetic drift in a finite population undergoing selection, without requiring other mechanisms. This is supported by recent simulations apparently demonstrating that, in small populations, drift is more important t...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:The Saccharomyces cerevisiae nuclear membrane is part of a complex nuclear envelope environment also containing chromatin, integral and peripheral membrane proteins, and large structures such as nuclear pore complexes (NPCs) and the spindle pole body. To study how properties of the nuclear membrane affect nuclear enve...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.119149
更新日期：2010-11-01 00:00:00
abstract：:Cytoplasmic male sterility (CMS), the maternally inherited failure to produce functional pollen, has been used in the breeding of sugar beet (Beta vulgaris ssp. vulgaris). At least three different sources of CMS can be distinguished from one another as well as from normal fertile cytoplasm by polymorphisms in their mi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.076380
更新日期：2007-11-01 00:00:00
abstract：:Sequences of Bronze2 (Bz2), a maize gene which is required for the synthesis of the purple pigment anthocyanin, have been cloned by combining the techniques of transposon tagging and differential hybridization. First, a mutable bz2 allele (bz2-mu1) was recovered from a Mutator line. The mutation was assumed to result ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:Associative overdominance, the fitness difference between heterozygotes and homozygotes at a neutral locus, is classically described using two categories of models: linkage disequilibrium in small populations or identity disequilibrium in infinite, partially selfing populations. In both cases, only equilibrium situati...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
