Abstract:
:The Saccharomyces cerevisiae nuclear membrane is part of a complex nuclear envelope environment also containing chromatin, integral and peripheral membrane proteins, and large structures such as nuclear pore complexes (NPCs) and the spindle pole body. To study how properties of the nuclear membrane affect nuclear envelope processes, we altered the nuclear membrane by deleting the SPO7 gene. We found that spo7Δ cells were sickened by the mutation of genes coding for spindle pole body components and that spo7Δ was synthetically lethal with mutations in the SUN domain gene MPS3. Mps3p is required for spindle pole body duplication and for a variety of other nuclear envelope processes. In spo7Δ cells, the spindle pole body defect of mps3 mutants was exacerbated, suggesting that nuclear membrane composition affects spindle pole body function. The synthetic lethality between spo7Δ and mps3 mutants was suppressed by deletion of specific nucleoporin genes. In fact, these gene deletions bypassed the requirement for Mps3p entirely, suggesting that under certain conditions spindle pole body duplication can occur via an Mps3p-independent pathway. These data point to an antagonistic relationship between nuclear pore complexes and the spindle pole body. We propose a model whereby nuclear pore complexes either compete with the spindle pole body for insertion into the nuclear membrane or affect spindle pole body duplication by altering the nuclear envelope environment.
journal_name
Geneticsjournal_title
Geneticsauthors
Witkin KL,Friederichs JM,Cohen-Fix O,Jaspersen SLdoi
10.1534/genetics.110.119149subject
Has Abstractpub_date
2010-11-01 00:00:00pages
867-83issue
3eissn
0016-6731issn
1943-2631pii
genetics.110.119149journal_volume
186pub_type
杂志文章相关文献
GENETICS文献大全abstract::A wheat (Triticum aestivum L. emend Thell) disomic addition line (2n = 6x = 44), SH1-152-2, with a pair of Elytrigia pontica (Podp.) Holub 2n = 10x = 70 [syn. Agropyron elongatum (Host) P.B.] chromosomes controlling blue aleurone color was crossed with a short-statured spring wheat ;Sonora 64' (T. aestivum). Isoline p...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Peroxisomes are organelles containing a diverse array of enzymes. In fungi they are important for carbon source utilization, pathogenesis, development, and secondary metabolism. We have studied Aspergillus nidulans peroxin (pex) mutants isolated by virtue of their inability to grow on butyrate or by the inactivation o...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.085795
更新日期:2008-03-01 00:00:00
abstract::The Arabidopsis short integuments 2-1 (sin2-1) mutant produces ovules with short integuments due to early cessation of cell division in these structures. SIN2 was isolated and encodes a putative GTPase sharing features found in the novel DAR GTPase family. DAR proteins share a signature DAR motif and a unique arrangem...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.060657
更新日期:2006-10-01 00:00:00
abstract::There are two naturally occurring functional alleles of the recombination hotspot cog, which is located 3.5 kb from the his-3 locus of Neurospora crassa. The presence of the cog+ allele in a cross significantly increases recombination in the his-3 region compared to a cross homozygous for the cog allele. Data obtained...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.025080
更新日期:2004-07-01 00:00:00
abstract::HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with > 80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::The existence of temporally stable frequency clines for In(2L)t in natural populations of Drosophila melanogaster suggests a role for selection in the maintenance of this polymorphism. We have collected nucleotide polymorphism data from the proximal breakpoint junction regions of In(2L)t to infer its evolutionary hist...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::A simple two-locus gene conversion model is considered to investigate the amounts of DNA variation and linkage disequilibrium in small multigene families. The exact solutions for the expectations and variances of the amounts of variation within and between two loci are obtained. It is shown that gene conversion increa...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::DnaB is the helicase associated with the DNA polymerase III replication fork in Escherichia coli. Previously we observed that the dnaB107(ts) mutation, at its permissive temperature, greatly stimulated deletion events at chromosomal tandem repeats. This stimulation required recA, which suggests a recombinational mecha...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::The Polymerase Associated Factor 1 complex (Paf1C) is a multifunctional regulator of eukaryotic gene expression important for the coordination of transcription with chromatin modification and post-transcriptional processes. In this study, we investigated the extent to which the functions of Paf1C combine to regulate t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302262
更新日期:2019-07-01 00:00:00
abstract::Genetic screens in Drosophila melanogaster and other organisms have been pursued to filter the genome for genetic functions important for memory formation. Such screens have employed primarily chemical or transposon-mediated mutagenesis and have identified numerous mutants including classical memory mutants, dunce and...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.173575
更新日期:2015-04-01 00:00:00
abstract::Position effect variegation results from chromosome rearrangements which translocate euchromatic genes close to the heterochromatin. The euchromatin-heterochromatin association is responsible for the inactivation of these genes in some cell clones. In Drosophila melanogaster the Y chromosome, which is entirely heteroc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::Results of immunogenetic, immunochemical and physicochemical investigations on two serum allotypes of swine are reported. The allotypes, designated Lpr1 and Lpr2, have been identified by specific alloprecipitins in agar gel. Genetic studies indicate that the allotypes are specified by two codominant autosomal allelic ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Mitochondrial complex I is the largest multimeric enzyme of the respiratory chain. The lack of a model system with facile genetics has limited the molecular dissection of complex I assembly. Using Chlamydomonas reinhardtii as an experimental system to screen for complex I defects, we isolated, via forward genetics, am...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.128827
更新日期:2011-06-01 00:00:00
abstract::Many insects feed on only one or a few types of host. These host specialists often evolve a preference for chemical cues emanating from their host and develop mechanisms for circumventing their host's defenses. Adaptations like these are central to evolutionary biology, yet our understanding of their genetics remains ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.154773
更新日期:2013-11-01 00:00:00
abstract::Heterochromatin protein 1a (HP1a) is a highly conserved and versatile epigenetic factor that can both silence and activate transcription. However, the function of HP1a in development has been underinvestigated. Here, we report the role of maternal HP1a in producing maternal transcripts that drive early Drosophila embr...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301704
更新日期:2019-01-01 00:00:00
abstract::Seventeen lines, each homozygous for a different X chromosome but all with a common autosomal genetic blackground, were constructed and assayed for abdominal bristle number to determine whether dosage compensation operates for sex-linked genes affecting this character. --The regression coefficient of male mean on fema...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::A maximum-likelihood method to estimate the recombination fraction and its sampling variance using informative and noninformative half-sib offspring is derived. Estimates of the recombination fraction are biased up to 20 cM when noninformative offspring are discarded. In certain scenarios, the sampling variance can be...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Magnesium (Mg) is an essential enzyme cofactor and a key structural component of biological molecules, but relatively little is known about the molecular components required for Mg homeostasis in eukaryotic cells. The yeast genome encodes four characterized members of the CorA Mg transporter superfamily located in the...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.106419
更新日期:2009-11-01 00:00:00
abstract::The L-shaped distribution of estimated QTL effects (R(2)) has long been reported. We recently showed that a metabolic mechanism could account for this phenomenon. But other nonexclusive genetic or nongenetic causes may contribute to generate such a distribution. Using analysis and simulations of an additive genetic mo...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::COTRANS is a program for analyzing cotransduction data. It calculates distances from pairwise cotransduction frequencies, computes crossovers required to obtain each observed recombinant class, and applies rules to draw conclusions about order. The rules are based on the correlation between the frequency of the classe...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Restriction fragment length polymorphisms in mitochondrial DNA (mtDNA) were used to reconstruct evolutionary relationships of conspecific populations in four species of freshwater fish-Amia calva, Lepomis punctatus, L. gulosus, and L. microlophus. A suite of 14-17 endonucleases was employed to assay mtDNAs from 305 sp...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Evolutionary rescue describes a situation where adaptive evolution prevents the extinction of a population facing a stressing environment. Models of evolutionary rescue could in principle be used to predict the level of stress beyond which extinction becomes likely for species of conservation concern, or, conversely, ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300908
更新日期:2018-05-01 00:00:00
abstract::The swi8+ gene of Schizosaccharomyces pombe appears to be involved in the termination step of copy synthesis during mating-type (MT) switching. Mutations in swi8 confer a general mutator phenotype and, in particular, generate specific mutations in the MT region. Sequencing of the MT cassettes of the h90 swi8-137 mutan...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::Mutations in arg-13 result in slow growth in minimal medium and can suppress mutations in carbamyl phosphate synthase-aspartate carbamyl transferase within the pyrimidine pathway; the exact biochemical function of the gene product is unknown. To understand the role of arg-13 in arginine metabolism, cosmids rescuing gr...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::We have previously isolated mutants of Escherichia coli that replicate their DNA with increased fidelity. These mutants have a mutation in the dnaE gene, encoding the alpha subunit of DNA polymerase III. They were isolated in a mismatch-repair-defective mutL background, in which mutations can be considered to represen...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::We have studied a genome-wide set of single-nucleotide polymorphism (SNP) allele frequency measures for African-American, East Asian, and European-American samples. For this analysis we derived a simple, closed mathematical formulation for the spectrum of expected allele frequencies when the sampled populations have e...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.1.351
更新日期:2004-01-01 00:00:00
abstract::Yip1p is the first identified Rab-interacting membrane protein and the founder member of the YIP1 family, with both orthologs and paralogs found in all eukaryotic genomes. The exact role of Yip1p is unclear; YIP1 is an essential gene and defective alleles severely disrupt membrane transport and inhibit ER vesicle budd...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.032888
更新日期:2004-12-01 00:00:00
abstract::The biological basis of exercise behavior is increasingly relevant for maintaining healthy lifestyles. Various quantitative genetic studies and selection experiments have conclusively demonstrated substantial heritability for exercise behavior in both humans and laboratory rodents. In the "High Runner" selection exper...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303668
更新日期:2020-11-01 00:00:00
abstract::The idealized individual in many fungal species is a haploid self-sterile hermaphrodite that may be propagated by asexually produced spores or that may reproduce sexually. In field populations, polymorphism occurs for female-sterile/hermaphrodite status, and female-sterile mutants, which function only as males during ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::Florida teosinte from Honduras and Guanajuato teosinte have most of their knobs internally located and a common inversion in the short arm of chromosome 8. Teosintes from northern Guatemala, Huixta and Monajil, have all their knobs terminally located and do not have any inversions. Therefore, Florida teosinte from Hon...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00