Abstract：
:Genetic screens in Drosophila melanogaster and other organisms have been pursued to filter the genome for genetic functions important for memory formation. Such screens have employed primarily chemical or transposon-mediated mutagenesis and have identified numerous mutants including classical memory mutants, dunce and rutabaga. Here, we report the results of a large screen using panneuronal RNAi expression to identify additional genes critical for memory formation. We identified >500 genes that compromise memory when inhibited (low hits), either by disrupting the development and normal function of the adult animal or by participating in the neurophysiological mechanisms underlying memory formation. We also identified >40 genes that enhance memory when inhibited (high hits). The dunce gene was identified as one of the low hits and further experiments were performed to map the effects of the dunce RNAi to the α/β and γ mushroom body neurons. Additional behavioral experiments suggest that dunce knockdown in the mushroom body neurons impairs memory without significantly affecting acquisition. We also characterized one high hit, sickie, to show that RNAi knockdown of this gene enhances memory through effects in dopaminergic neurons without apparent effects on acquisition. These studies further our understanding of two genes involved in memory formation, provide a valuable list of genes that impair memory that may be important for understanding the neurophysiology of memory or neurodevelopmental disorders, and offer a new resource of memory suppressor genes that will aid in understanding restraint mechanisms employed by the brain to optimize resources.
journal_name
Geneticsjournal_title
Geneticsauthors
Walkinshaw E,Gai Y,Farkas C,Richter D,Nicholas E,Keleman K,Davis RLdoi
10.1534/genetics.114.173575subject
Has Abstractpub_date
2015-04-01 00:00:00pages
1173-82issue
4eissn
0016-6731issn
1943-2631pii
genetics.114.173575journal_volume
199pub_type
杂志文章相关文献
GENETICS文献大全abstract：:A radiation-induced translocation, T(IIR----IIIL), has been shown to be nonreciprocal and to have most of IIR, including its terminus, attached uninverted to the terminus of IIIL.--Progeny with the IIR segment in duplicate, obtained from crosses of T(IIR----IIIL) to strains with a standard genome, were unstable at mit...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Hydroxyurea (HU) has been used for the treatment of multiple diseases, such as cancer. The therapeutic effect is generally believed to be due to the suppression of ribonucleotide reductase (RNR), which slows DNA polymerase movement at replication forks and induces an S phase cell cycle arrest in proliferating cells. A...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.191536
更新日期：2016-11-01 00:00:00
abstract：:PGM plays a central role in the glycolytic pathway at the branch point leading to glycogen metabolism and is highly polymorphic in allozyme studies of many species. We have characterized the nucleotide diversity across the Pgm gene in Drosophila melanogaster and D. simulans to investigate the role that protein polymor...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:Hairpin-tail (T(hp)) is a new allele of brachyury on chromosome 17 (linkage group IX) of the mouse. The morphological effects in the heterozygote are on the notochord and the tail. The homozygous abnormal is unknown. T(hp) is unique in that the phenotype of the heterozygote seems to depend on the source of the T(hp) g...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-04-01 00:00:00
abstract：:The Drosophila melanogaster Gld gene has multiple and diverse developmental and physiological functions. We report herein that interactions among proximal promoter elements and a cluster of intronically located enhancers and silencers specify the complex regulation of Gld that underlies its diverse functions. Gld expr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:Likelihood-based parentage inference depends on the distribution of a likelihood-ratio statistic, which, in most cases of interest, cannot be exactly determined, but only approximated by Monte Carlo simulation. We provide importance-sampling algorithms for efficiently approximating very small tail probabilities in the...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.048074
更新日期：2006-04-01 00:00:00
abstract：:We compared allele sequences of two loci near the Arabidopsis lyrata self-incompatibility (S) loci with sequences of A. thaliana orthologs and found high numbers of shared polymorphisms, even excluding singletons and sites likely to be highly mutable. This suggests maintenance of entire S-haplotypes for long evolution...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.051938
更新日期：2006-04-01 00:00:00
abstract：:Pairwise linkage disequilibrium values (D) were estimated for 14 allozyme loci in two natural populations of lodgepole pine (Pinus contorta ssp. latifolia). Maternal multilocus genotypes were inferred from samples of (haploid) megagametophytic seed-endosperms. Coupling/repulsion double heterozygotes were distinguished...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:Mutations affecting the self-incompatibility response of Nicotiana alata were generated by irradiation. Mutants in the M1 generation were selected on the basis of pollen tube growth through an otherwise incompatible pistil. Twelve of the 18 M1 plants obtained from the mutagenesis screen were self-compatible. Eleven se...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:N6-methyladenosine (m6A), catalyzed by Mettl3 methyltransferase, is a highly conserved epigenetic modification in eukaryotic messenger RNA (mRNA). Previous studies have implicated m6A modification in multiple biological processes, but the in vivo function of m6A has been difficult to study, because mettl3 mutants are ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300574
更新日期：2018-02-01 00:00:00
abstract：:The distribution of four X-linked mutants (G6PD, Deutan, Protan and Xg) among lowland and once highly malarial populations of Sardinia discloses a clear-cut example of linkage disequiligrium between two of them (G6PD and Protan). In the same populations the distribution of G6PD-deficiency versus colorblindness of the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Research on the genetics of natural populations was revolutionized in the 1990s by methods for genotyping noninvasively collected samples. However, these methods have remained largely unchanged for the past 20 years and lag far behind the genomics era. To close this gap, here we report an optimized laboratory protocol...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.187492
更新日期：2016-06-01 00:00:00
abstract：:Sequence divergence among the mitochondrial (mt) DNA of nine Lycopersicon and two closely related Solanum species was estimated using the shared fragment method. A portion of each mt genome was highlighted by probing total DNA with a series of plasmid clones containing mt-specific DNA fragments from Lycopersicon penne...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:The cut gene in Drosophila is necessary in at least one cell type, the external sensory organs, for proper cell type specification and morphogenesis. It is also expressed in a variety of other tissues, where its function is less well characterized. Previous work has demonstrated that mutations affecting all the tissue...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:A simple method of replica plating has been used to isolate mutants of Neurospora crassa that have increased sensitivity to methyl methanesulfonate (MMS) and/or to histidine. Twelve mutants with increased sensitivity to MMS and one mutant with increased sensitivity to histidine showed Mendelian segregation of the muta...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:We have mapped quantitative trait loci (QTL) for Drosophila mechanosensory bristle number in six recombinant isogenic line (RIL) mapping populations, each of which was derived from an isogenic chromosome extracted from a line selected for high or low, sternopleural or abdominal bristle number and an isogenic wild-type...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:We consider the effect on the distribution of pairwise differences between mitochondrial DNA sequences of the incorporation into the underlying population genetics model of two particular effects that seem realistic for human populations. The first is that the population size was roughly constant before growing to its...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The distribution of genetic polymorphisms in a population contains information about evolutionary processes. The Poisson random field (PRF) model uses the polymorphism frequency spectrum to infer the mutation rate and the strength of directional selection. The PRF model relies on an infinite-sites approximation that i...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.087361
更新日期：2008-12-01 00:00:00
abstract：:X chromosome duplications have been used previously to vary the dose of specific regions of the X chromosome to study dosage compensation and sex determination in Caenorhabditis elegans. We show here that duplications suppress and X-linked hypomorphic mutation and elevate the level of activity of an X-linked enzyme, a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Numerous loci in host organisms are involved in parasite recognition, such as major histocompatibility complex (MHC) genes in vertebrates or genes involved in gene-for-gene (GFG) relationships in plants. Diversity is commonly observed at such loci and at corresponding loci encoding antigenic molecules in parasites. Mu...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.074393
更新日期：2007-11-01 00:00:00
abstract：:Many of the transcription factors and target genes that pattern the developing adult remain unknown. In the present study, we find that an ortholog of the poorly understood transcription factor, glucose transporter (GLUT4) enhancer factor (Glut4EF, GEF) [also known as the Huntington's disease gene regulatory region-bi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.078030
更新日期：2008-02-01 00:00:00
abstract：:The back-to-back geometry of sister kinetochores is essential in preventing loss or damage of chromosomes during mitosis. Kinetochore orientation is generated in part by a process of resolving kinetochores at the centromere (centromere resolution) prior to spindle interactions. Because few of the genes required for ce...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.075275
更新日期：2008-01-01 00:00:00
abstract：:Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosom...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.026617
更新日期：2004-09-01 00:00:00
abstract：:Mutant alleles of two genetic regulatory elements, which underlie a three- to sixfold reduction in beta-glucuronidase (GUS) activity levels, distinguish mice of the H haplotype from those of the other two common GUS haplotypes, A and B. Both elements are tightly linked to the GUS structural gene over which they exert ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The K1 killer plasmid, [KIL-k1], of Saccharomyces cerevisiae is a 1.25 x 10(6) dalton linear double-stranded RNA plasmid coding for a protein toxin and immunity to that toxin. The [KIL-sd1] plasmid is a replication-defective mutant of [KIL-k1] that depends on one of the recessive chromosomal superkiller (ski-) mutatio...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:Although neurons are highly polarized, how neuronal polarity is generated remains poorly understood. An evolutionarily conserved inositol-producing enzyme myo-inositol monophosphatase (IMPase) is essential for polarized localization of synaptic molecules in Caenorhabditis elegans and can be inhibited by lithium, a dru...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.137844
更新日期：2012-06-01 00:00:00
abstract：:Many heterogametic organisms adjust sex chromosome expression to accommodate differences in gene dosage. This requires selective recruitment of regulatory factors to the modulated chromosome. How these factors are localized to a chromosome with requisite accuracy is poorly understood. Drosophila melanogaster males inc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301173
更新日期：2018-08-01 00:00:00
abstract：:Mitochondria of Neurospora crassa contain a cyanide-resistant alternative respiratory pathway in addition to the cytochrome pathway. The alternative oxidase is present only when electron flow through the cytochrome chain is restricted. Both genomic and cDNA copies for the alternative oxidase gene have been isolated an...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:We propose a simple algorithm for estimating the number of nucleotide differences between a pair of RNA or DNA sequences through comparison of their RNAse A mismatch cleavage patterns. In the RNAse A mismatch cleavage technique two or more sample sequences are hybridized to the same RNA probe, the hybrids are partiall...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:We extensively phenotyped 6000 Arabidopsis plants with experimentally perturbed DNA methylomes as well as a diverse panel of natural accessions in a common garden. We found that alterations in DNA methylation not only caused heritable phenotypic diversity but also produced heritability patterns closely resembling thos...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.128744
更新日期：2011-08-01 00:00:00
