Quantitative histochemistry of cytochrome oxidase in rat brain.

Abstract:

:A quantitative analysis of cytochrome oxidase (CO) activity in histochemically stained sections of rat brain was developed using tissue standards and computerized image processing. Standards of brain paste containing known amounts of CO were cryosectioned and stained under the same conditions as brain sections. The gray levels of the stain were converted to units of CO activity using a calibration curve derived from densitometric analysis of the standards. The technique yields reproducible quantitative values, has the superior anatomical resolution of histochemistry, and is compatible with autoradiography.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Gonzalez-Lima F,Garrosa M

doi

10.1016/0304-3940(91)90943-n

subject

Has Abstract

pub_date

1991-02-25 00:00:00

pages

251-3

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(91)90943-N

journal_volume

123

pub_type

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