CDC7 kinase phosphorylates serine residues adjacent to acidic amino acids in the minichromosome maintenance 2 protein.

abstract：:The baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV, which is representative of the MNPV subtype in which the virions may contain many nucleocapsids within a single viral envelope) encodes a protein, v-ubi, that has 76% identity with the eukaryotic protein ubiquitin. Transcriptional mapping indic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guarino LA

更新日期：1990-01-01 00:00:00

abstract：:The Pleistocene global dispersal of modern humans required the transit of arid and semiarid regions where the distribution of potable water provided a primary constraint on dispersal pathways. Here, we provide a spatially explicit continental-scale assessment of the opportunities for Pleistocene human occupation of Au...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bird MI,O'Grady D,Ulm S

更新日期：2016-10-11 00:00:00

abstract：:We have examined the effects of a stromal cell-derived cytokine designated interleukin 11 (IL-11) on the proliferation of murine hemopoietic progenitors in methylcellulose culture. COS cell-conditioned medium containing IL-11 supported formation of granulocyte/macrophage colonies and a small number of multilineage col...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musashi M,Yang YC,Paul SR,Clark SC,Sudo T,Ogawa M

更新日期：1991-02-01 00:00:00

abstract：:We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH(+)) dopaminergic neurons, from mouse embryonic stem cells. We report here that SDIA induces efficient neural differentiation also in primate embr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawasaki H,Suemori H,Mizuseki K,Watanabe K,Urano F,Ichinose H,Haruta M,Takahashi M,Yoshikawa K,Nishikawa S,Nakatsuji N,Sasai Y

更新日期：2002-02-05 00:00:00

abstract：:Cell clones nonproductively transformed by the replication-defective Abelson strain of murine leukemia virus (AbLV) were analyzed for type C viral antigen expression by competition immunoassay. AbLV-transformed mink non-producer lines were found to express a 110,000- to 130,000-molecular weight polyprotein containing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds FH Jr,Sacks TL,Deobagkar DN,Stephenson JR

更新日期：1978-08-01 00:00:00

abstract：:The freezing tolerance of protoplasts isolated from nonacclimated rye leaves (Secale cereale L. cv Puma) was significantly altered by using a pH-induced protoplastliposome fusion technique to modify the lipid composition of the plasma membrane. The increase in freezing tolerance was elicited by fusion with liposomes c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steponkus PL,Uemura M,Balsamo RA,Arvinte T,Lynch DV

更新日期：1988-12-01 00:00:00

abstract：:Preparations of partially purified chloroplast DNA-dependent RNA polymerase from maize and some other plants transcribe cloned chloroplast genes preferentially and much more actively from appropriately negatively supercoiled templates than from relaxed templates. We have found that the polymerase in such fractions doe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaitlin D,Hu J,Bogorad L

更新日期：1989-02-01 00:00:00

abstract：:The hallmark of the menstrual cycle is extensive steroid-dependent tissue turnover. Estrogen mediates endometrial cell growth and structural remodeling, whereas progesterone suppresses estrogen-dependent proliferation and promotes cellular differentiation. In nonfertile cycles, tissue degradation and menstruation occu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Osteen KG,Rodgers WH,Gaire M,Hargrove JT,Gorstein F,Matrisian LM

更新日期：1994-10-11 00:00:00

abstract：:Saccharomyces cerevisiae possesses a high-affinity estrogen binding protein and an endogenous ligand that displaces [3H]estradiol from both the yeast binding protein and mammalian estrogen receptors. Semipurified preparations of this ligand have been shown to exhibit estrogenic activity in mammalian systems. We now de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldman D,Tökés LG,Stathis PA,Miller SC,Kurz W,Harvey D

更新日期：1984-08-01 00:00:00

abstract：:We have isolated rat brain cDNA clones encoding AE2, a homologue of the erythrocyte anion exchanger, band 3 (AE1). Immunocytochemistry and in situ hybridization reveal that, in brain, AE2 expression is restricted to the basolateral membrane of the choroid plexus epithelium. Expression of a full-length mouse AE2 cDNA i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindsey AE,Schneider K,Simmons DM,Baron R,Lee BS,Kopito RR

更新日期：1990-07-01 00:00:00

abstract：:The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion. We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruce WB,Christensen AH,Klein T,Fromm M,Quail PH

更新日期：1989-12-01 00:00:00

abstract：:Wnt/beta-catenin signaling inhibits adipogenesis. Genome-wide profiling studies have revealed the enrichment of histone H3K27 methyltransferase Ezh2 on Wnt genes. However, the functional significance of such a direct link between the two types of developmental regulators in mammalian cells, and the role of Ezh2 in adi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Jin Q,Lee JE,Su IH,Ge K

更新日期：2010-04-20 00:00:00

abstract：:As the Conus shell grows by spiraling of the outer lip around the axis, profound internal shell dissolution thins the walls of the protected penultimate whorl from several millimeters to <50mum. Shell material is added to the inside of the spire and the anterior part of the columella. The resulting shell has a uniform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kohn AJ,Myers ER,Meenakshi VR

更新日期：1979-07-01 00:00:00

abstract：:The synthesis of antiviral beta-chemokines has joined cytolysis as a potential mechanism for the control of HIV-1 infection by CD8(+) T cells. Recent evidence suggests that these two effector functions can diverge in some individuals infected with HIV-1; however, little is known about the CD8(+) T cell subsets in norm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamin-Lewis R,Abdelwahab SF,Trang C,Baker A,DeVico AL,Gallo RC,Lewis GK

更新日期：2001-07-31 00:00:00

abstract：:It is well known that two regular tetrahedra can be combined with a single regular octahedron to tile (complete fill) three-dimensional Euclidean space . This structure was called the "octet truss" by Buckminster Fuller. It was believed that such a tiling, which is the Delaunay tessellation of the face-centered cubic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conway JH,Jiao Y,Torquato S

更新日期：2011-07-05 00:00:00

abstract：:Molecular cloning has demonstrated a diversity of artificially expressed N-methyl-D-aspartate (NMDA) receptors, implying a similar diversity of naturally occurring NMDA receptors in situ. Particularly significant was the success in expression of NMDA receptor classes exhibiting various sensitivities to Mg2+ block, a v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kato N,Yoshimura H

更新日期：1993-08-01 00:00:00

abstract：:Molecular clocks have been used to date the divergence of humans and chimpanzees for nearly four decades. Nonetheless, this date and its confidence interval remain to be firmly established. In an effort to generate a genomic view of the human-chimpanzee divergence, we have analyzed 167 nuclear protein-coding genes and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar S,Filipski A,Swarna V,Walker A,Hedges SB

更新日期：2005-12-27 00:00:00

abstract：:Four overlapping cosmid clones were constructed that contain the complete genome of the attenuated Oka strain of VZV. Transfection of human melanoma cells with the four cosmids resulted in production of infectious VZV. A double-stranded oligonucleotide, encoding a stop codon in all three open reading frames, was inser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen JI,Seidel KE

更新日期：1993-08-01 00:00:00

abstract：:Functional transcription elongation complexes can be formed by adding RNA polymerase in trans to a preformed nucleic acid construct. This construct consists of a double-stranded DNA fragment that contains a noncomplementary (permanent DNA bubble) region into which an RNA primer oligonucleotide has been hybridized. By ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daube SS,Hart CR,von Hippel PH

更新日期：1994-09-27 00:00:00

abstract：:Protein-mediated electron transfer is an essential event in many biochemical processes. Efficient electron transfer requires the reorganization energy of the redox event to be minimized, which is ensured by the presence of rigid donor and acceptor sites. Electron transfer copper sites are present in the ubiquitous cup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaballa ME,Abriata LA,Donaire A,Vila AJ

更新日期：2012-06-12 00:00:00

abstract：:Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The (1)H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Putter I,Barreto A,Markley JL,Jardetzky O

更新日期：1969-12-01 00:00:00

abstract：:Translation of injected mRNA in oocytes of Xenopus laevis has been used as a highly sensitive and quantitative assay for interferon mRNA. Injection into oocytes of polyadenylylated RNA extracted from poly(I).poly(C)-induced human diploid fibroblasts (FS-4) leads to the synthesis of biologically active human fibroblast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sehgal PB,Dobberstein B,Tamm I

更新日期：1977-08-01 00:00:00

abstract：:In the mammalian visual system, the terminal arbors of retinal ganglion cell axons from the two eyes are restricted to mutually exclusive territories within their thalamic target, the lateral geniculate nucleus (LGN). Here we have investigated some of the factors that determine the adult morphology of terminal arbors ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garraghty PE,Shatz CJ,Sretavan DW,Sur M

更新日期：1988-10-01 00:00:00

abstract：:Cells are nonequilibrium systems that exchange matter and energy with the environment to sustain their metabolic needs. The nonequilibrium nature of this system presents considerable challenges to developing a general theory describing its behavior; however, when studied at appropriate spatiotemporal scales, the behav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang AY,Marshall WF

更新日期：2019-10-22 00:00:00

abstract：:In HL-60 cells, a human promyelocytic leukemia cell line, the human c-myc gene, designated MYC, is amplified about 16-fold. On differentiation of the HL-60 cells into granulocytes induced by several inhibitors of poly(ADP-ribose) polymerase [NAD+ poly(adenosine diphosphate D-ribose)ADP-D-ribosyltransferase, EC 2.4.2.3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima H,Nakayasu M,Aonuma S,Sugimura T,Nagao M

更新日期：1989-10-01 00:00:00

abstract：:One of the principles that has emerged from experimental evolutionary studies of microorganisms is that polymorphic alleles or new mutations can sometimes possess a latent potential to respond to selection in different environments, although the alleles may be functionally equivalent or disfavored under typical condit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stebbins GL,Hartl DL

更新日期：1988-07-01 00:00:00

abstract：:Comparison of about 50 pairs of homologous nucleotide sequences for different genes revealed that the substitutions between synonymous codons occurred at much higher rates than did amino acid substitutions. Furthermore, five pairs of mRNA sequences for different genes were compared in species that had diverged at the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyata T,Yasunaga T,Nishida T

更新日期：1980-12-01 00:00:00

abstract：:A discrepancy of about 20% exists between the molecular weight of the alpha subunit of Torpedo californica electroplax acetylcholine receptor as determined by gel electrophoresis of the mature protein (Mr 40,000 +/- 2000) and by nucleotide sequence analysis of cDNA (Mr approximately equal to 50,000). We demonstrate by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conti-Tronconi BM,Hunkapiller MW,Raftery MA

更新日期：1984-05-01 00:00:00

abstract：:Antarctic ozone depletion is associated with enhanced chlorine from anthropogenic chlorofluorocarbons and heterogeneous chemistry under cold conditions. The deep Antarctic "hole" contrasts with the generally weaker depletions observed in the warmer Arctic. An unusually cold Arctic stratospheric season occurred in 2011...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Solomon S,Haskins J,Ivy DJ,Min F

更新日期：2014-04-29 00:00:00

abstract：:We have investigated the effect of the v-Myc oncoprotein on gene expression in myelomonocytic cells. We find that v-Myc dramatically down-regulates the expression of myelomonocytic-specific genes, such as the chicken mim-1 and lysozyme genes, both of which are known targets for C/EBP transcription factors. We present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mink S,Mutschler B,Weiskirchen R,Bister K,Klempnauer KH

更新日期：1996-06-25 00:00:00