Striatal glutamic acid decarboxylase immunoreactivity is increased after dopaminergic deafferentation: densitometric analysis.

Abstract:

:Several lines of evidence suggest that dopamine exerts a chronic inhibitory action on GABAergic cells in the striatum, and striatal glutamic acid decarboxylase (GAD) mRNA levels are increased after ipsilateral dopaminergic denervation. In the present study we have used GAD immunocytochemistry to assess whether dopaminergic denervation results in an increase in GAD protein synthesis. In three 6-hydroxydopamine-lesioned animals, there was a perceptible increase in the density of GAD-immunoreactive (ir)staining on the side ipsilateral to the lesion. Computer-assisted densitometric analysis showed a significant increase in GAD-ir staining in the ipsilateral striatum compared to the contralateral (control) side. These data suggest that removal of striatal dopaminergic innervation results in an increase in the amount of immunoreactive GAD, the rate limiting enzyme in the synthesis of GABA.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Segovia J,Armstrong DM,Benzing WC,Hornby PJ

doi

10.1016/0304-3940(91)90871-p

subject

Has Abstract

pub_date

1991-01-28 00:00:00

pages

252-6

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(91)90871-P

journal_volume

122

pub_type

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