Abstract:
:Neuronal nAChRs are pentameric transmembrane proteins which function as ligand-gated ion channels and are composed of multiple alpha and beta subunits. Nine neuronal nAChR alpha subunit genes (alpha2-alpha10) and three nAChR beta subunit genes (beta2-beta4) have been identified. nAChR subtypes are heteromers, composed of various combinations of nAChR subunits or homomers composed of alpha7, alpha8, or alpha9 subunits. nAChR subtypes are widely expressed in the nervous system, yet each subunit has a distinct and unique pattern of expression. This report focuses on the expression of the nAChR alpha7 gene since homomeric nAChRs can be formed from this one subunit, simplifying a study of the expression of a specific nAChR subtype. Alpha7 nAChRs are involved in several important biological activities in addition to synaptic transmission including mediating neurite outgrowth, neuronal development and cell death, and in presynaptic control of neurotransmitter release. Transcriptional regulation of alpha7 gene expression may be important to control the location and timing of these events. We previously isolated a rat alpha7 nAChR promoter and studied expression in PC12 cells. In this study we examined the expression of the alpha7 promoter in PC12, HEK293, L6, SN17 and Neuro-2A cells in order to define elements necessary for cell-specific expression. Elements promoting expression of alpha7 in muscle and fibroblasts were identified. We also demonstrated that several other nAChR genes are also expressed in SN 17 and Neuro-2A cells, supporting use of these cell lines as models to study transcriptional control of nAChR genes.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Danthi S,Boyd RTdoi
10.1016/j.neulet.2006.02.067keywords:
subject
Has Abstractpub_date
2006-05-29 00:00:00pages
63-8issue
1-2eissn
0304-3940issn
1872-7972pii
S0304-3940(06)00146-7journal_volume
400pub_type
杂志文章abstract::Chondroitin sulfate/dermatan sulfate (CS/DS) polysaccharides have been reported to play a crucial role in the proliferation and maintenance of neural stem cells (NSCs). However, little is known about the structural changes and functional role of CS/DS chains in the differentiation of NSCs. Western blots of NSCs, neuro...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.02.019
更新日期:2011-04-15 00:00:00
abstract::In this study the effect of exogenous oxytocin and of massage-like stroking on the withdrawal latency responses to heat and mechanical nociceptive stimulation were investigated in rats. A hot-plate test and the Randall-Selitto test were used to assess the withdrawal responses. Exogenous oxytocin (0.1-1 mg/kg) and stro...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11335-t
更新日期:1995-02-24 00:00:00
abstract::Recent evidence has suggested that down-regulation of somatostatin (SST) expression in the human brain during early stages of aging leads to an elevation in the steady-state levels of Abeta and therefore may be involved in Alzheimer's disease (AD) progression. We hypothesized that alterations in the SST gene might alt...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.09.002
更新日期:2009-11-13 00:00:00
abstract::Rats subjected to forced oral self-administration of morphine solutions without or in combination with two daily i.p. injections of morphine preferred drinking water when this was offered in addition to morphine solutions. The daily intake of morphine during the terminal phase of self-administration of morphine was 50...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90476-n
更新日期:1992-04-13 00:00:00
abstract::We examined the intracellular Ca2+ ([Ca2+]i) elevation evoked by GABA in an 8-day embryonic chick brainstem slice using a Ca imaging technique with Ca green-1 AM. When small quantities of GABA were pressure-ejected on the surface of the slice, the [Ca2+]i elevation was clearly detected. The GABA-induced [Ca2+]i elevat...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.10.019
更新日期:2007-01-03 00:00:00
abstract::Circadian rhythms of pineal melatonin release were measured in freely moving pigeons (Columba livia) by in vivo microdialysis. The birds were placed in light-dark cycles with 12 h of light and 12 h of darkness (LD 12:12) or continuous dim light (LLdim) after LD 12:12. Although the level of melatonin was various, daily...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90811-k
更新日期:1992-12-14 00:00:00
abstract::The pathogens of Alzheimer's disease (AD) are still unclear, while accumulating evidences have indicated that both genetic and environmental factors are involved in the pathogenesis of AD. Recent studies suggest that AD is primarily a vascular disorder and copper (Cu) may play an important role in AD pathology. Howeve...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.04.030
更新日期:2012-06-14 00:00:00
abstract::Spinal cord injury (SCI) results in rapid and significant oxidative stress. We have previously demonstrated that polyethylene glycol (PEG) repairs neuronal membrane and inhibits lipid peroxidation in an in vitro model of SCI. In this study we tested the effects of PEG on oxidative stress in guinea pigs after SCI. Oxid...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.02.027
更新日期:2004-04-15 00:00:00
abstract::In this study we determined that cholinergic neurons from the lateral dorsal tegmental (LDT) and peribrachial pontine region (PPG) innervate the medial pontine reticular formation (medial PRF), a region involved in the generation of REM sleep. Wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) was injec...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90625-8
更新日期:1988-12-19 00:00:00
abstract::A two pulse technique was used to study the decay of mechanical activation in TTX poisoned mouse extensor digitorum longus (EDL) and soleus muscle fibres. A two microelectrode point voltage clamp technique was used with visual endpoint determination of the threshold potential for activation. The excitatory effect of a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(79)96152-4
更新日期:1979-10-01 00:00:00
abstract::Muscle and cutaneous nerves were individually labeled with DiI in chick embryos to examine the development of sensory afferent arborizations in the spinal cord. Initially, cutaneous and muscle arbors were similar; both types first entered the spinal gray matter at stage 28-29 (embryonic day (E) 6). Differences in proj...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90475-m
更新日期:1992-04-13 00:00:00
abstract::Sleep disturbance is common in chronic post-traumatic stress disorder (PTSD). However, prior work has demonstrated that there are inconsistencies between subjective and objective assessments of sleep disturbance in PTSD. Therefore, we investigated whether subjective or objective sleep assessment has greater clinical u...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.04.042
更新日期:2017-05-22 00:00:00
abstract::The phosphatidylinositol (PI) and phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolytic activities of phosphoinositide-specific phospholipase C (PLC) were measured in membrane and cytosol fractions from 7 discrete areas of the rat brain. Both the PI-PLC and PIP2-PLC specific activities were found to differ significa...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90215-7
更新日期:1988-08-01 00:00:00
abstract::Novel paired ependymal ridges (EPRS) have been identified in the ventrolateral wall of the cerebral aqueduct of the rat. Scanning electron microscopic techniques revealed that the dorsolateral and ventromedial ridges differ from each other in surface morphology. Using the PAP immunohistochemical technique, the EPRS de...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90196-8
更新日期:1981-11-18 00:00:00
abstract::Recent evidence has been presented demonstrating that group III mechanoreceptors comprise an important part of the sensory arm of the exercise pressor reflex, which in turn functions to increase arterial blood flow to contracting skeletal muscles. Although group III afferents are stimulated by mechanical distortion of...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.09.013
更新日期:2013-10-25 00:00:00
abstract::Facilitation of synaptic connections between sensory neurons and motor neurons mediating the tail-withdrawal reflex in Aplysia is produced by sensitizing stimuli. These effects can be mimicked by perfusing the pleural and pedal ganglia of a semi-intact preparation with serotonin (5-HT). In addition to the synaptic fac...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(84)90342-2
更新日期:1984-11-23 00:00:00
abstract::The present study was designed to determine whether the p53 tumor-suppressor protein is involved in the development of antinociceptive tolerance to morphine. When the doses of morphine (mg/kg per injection) were subcutaneously given into mice as pretreatment twice daily for 2 days (first day (30) and second day (60)),...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.11.061
更新日期:2009-02-06 00:00:00
abstract::Noradrenaline enhanced in a concentration-dependent way the basal release of endogenous GABA from superfused rat hippocampus synaptosomes. The alpha 2-adrenoceptor antagonist yohimbine prevented the releasing effect of noradrenaline while the alpha 1-adrenoceptor antagonist prazosin was ineffective. It is concluded th...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90430-7
更新日期:1987-05-19 00:00:00
abstract::We have previously shown that D-cycloserine displaces [3H]glycine binding to a recognition site with properties consistent with an N-methyl-D-aspartate (NMDA) receptor modulatory site. Additionally, D-cycloserine positively modulates the NMDA receptor as evidenced by its dose-dependent enhancement of [3H]1-[1-(2-thien...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90379-0
更新日期:1989-03-13 00:00:00
abstract::We developed a novel procedure for efficient mutagenesis of zebrafish using a DNA cross-linking agent 4,5',8-trimethylpsoralen (TMP), which is known to frequently induce small deletions in Escherichia coli and Caenorhabditis elegans. A specific-locus test and pilot screenings indicated that the TMP mutagenesis procedu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00132-3
更新日期:1998-03-13 00:00:00
abstract::The present study was aimed at investigating the role of gastrin in startle, startle habituation and prepulse inhibition (PPI). There were no significant differences between gastrin knockout mice and their wildtype controls in any of these baseline parameters. The disruption of PPI by treatment with 5 mg/kg of ampheta...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.10.013
更新日期:2005-01-20 00:00:00
abstract::1-methyl-4-phenylpyridinium ion (MPP(+)) has been shown to selectively inhibit mitochondrial function and induce a parkinsonism-like syndrome. MPP(+) stimulates the production of reactive oxygen species (ROS) and induces cell death in vitro. In this study, we investigated the protective effects of okadaic acid on MPP(...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.10.103
更新日期:2009-01-09 00:00:00
abstract::Quantitative neurophysiological studies have identified the presence of at least two spinal neuron populations (ABRUPT and SUSTAINED) which are excited by the noxious visceral stimulus colorectal distension (CRD). The present study examined the effects of acute colorectal inflammation on the activity of dorsal horn ne...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)00832-6
更新日期:2000-03-10 00:00:00
abstract::N-Methyl-D-aspartate (NMDA), quisqualate, kainate and potassium increased, in a dose-dependent manner, the efflux of radioactivity from rat cerebral cortex minislices preincubated with [3H]choline. Ketamine (1-5 microM) and magnesium (0.1-1 mM) reduced only the release evoked by NMDA. The non-parallel shift of the NMD...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(85)90271-x
更新日期:1985-05-23 00:00:00
abstract::Effective treatments to improve survivability following exposure to the nerve agent soman have been established and are currently available. Unfortunately, electrographic brain seizures, neuroinflammation and brain cell death are still a potential problem even with treatment. In the present study we have characterized...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00818-8
更新日期:2003-10-09 00:00:00
abstract::Fluorescent tracers (Nuclear Yellow, True Blue, Fast Blue) were injected, in various combinations, into the globus pallidus and substantia nigra in three marmosets. Examination of perikarya in the ipsilateral neostriatum, well removed from pallidal injection sites, revealed that the predominant pattern was of single-l...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(84)90127-7
更新日期:1984-08-24 00:00:00
abstract::Alpha-synuclein (α-SYN) is found in peripheral autonomic neuronal network apart from brain in Parkinson's disease (PD). Nitrated α-SYN is an undesirable modification associated with oxidative and nitrative damage and has been found extensively in brain, gastrointestinal(GI) tract and blood cells in PD. We aim to inves...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2019.03.054
更新日期:2019-06-21 00:00:00
abstract::The purpose of the present study was to investigate reports that the expression of dopamine agonist-induced behaviours is dependent upon the ratio of D1 to D2 receptor activation. Selective inactivation of the D1 dopamine receptor was achieved using the irreversible antagonist N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquin...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90521-a
更新日期:1990-07-17 00:00:00
abstract::The extracellular homeostasis of glutamate in the brain is maintained by the efficient uptake into astroglial cells. The high extracellular glutamate levels seen during seizures are therefore probably a result of both an increased synaptic release and a deranged glutamate uptake. In this study we used immuno-blotting ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01284-2
更新日期:2000-08-11 00:00:00
abstract::Activated microglia surround degenerating substantia nigra neurons in Parkinson's disease (PD). Such microglia produce high levels of interleukin-1 beta (IL-1 beta) and interleukin-1 alpha (IL-1 alpha). T and C alleles exist for the IL-1 beta-511 regulatory region as well as for the IL-1 alpha-889 regulatory region. T...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)00300-2
更新日期:2002-06-21 00:00:00