Abstract:
BACKGROUND:Early events in the progression of 90% of sporadic colorectal cancers depend on constitutive activation of Wnt signalling. Recent data also indicate a close association between the Hedgehog (Hh) and Wnt pathways in colonic epithelial cell differentiation. AIMS:To investigate whether expression of Gli1, a transactivator of Hh signalling, can suppress Wnt signalling and inhibit proliferation of human colorectal cancer cells. METHODS:Gli1 and nuclear beta-catenin expression were examined in a series of 40 human colorectal cancers by immunohistochemistry. We quantified Gli1 and nuclear beta-catenin staining as markers of Hh and Wnt pathway activation, respectively. Two human colon cancer cell lines, SW480 and HCT116, with mutations in APC and beta-catenin, respectively, were used. The effects of Gli1 overexpression on Wnt transcriptional activity, beta-catenin subcellular distribution, and proliferation in these cells were analysed. RESULTS:Nuclear accumulation of beta-catenin and the Gli1 staining level were inversely associated in the 40 human colorectal cancers. Wnt transcriptional activity was reduced in Gli1 transfected cells. These effects were observed even in Gli1 transfected cells cotransfected with mutated beta-catenin. Furthermore, nuclear accumulation of beta-catenin was diminished compared with that in empty vector transfected cells, and downregulated transcription of c-Myc was observed in Gli1 transfected cells. Proliferation of Gli1 transfected cells was also significantly suppressed compared with that in empty vector transfected cells. CONCLUSIONS:Our data suggest that Gli1 plays an inhibitory role in the development of colorectal cancer involving Wnt signalling, even in cases with the stabilising mutation of beta-catenin.
journal_name
Gutjournal_title
Gutauthors
Akiyoshi T,Nakamura M,Koga K,Nakashima H,Yao T,Tsuneyoshi M,Tanaka M,Katano Mdoi
10.1136/gut.2005.080333keywords:
subject
Has Abstractpub_date
2006-07-01 00:00:00pages
991-9issue
7eissn
0017-5749issn
1468-3288pii
gut.2005.080333journal_volume
55pub_type
杂志文章相关文献
GUT文献大全abstract::Acute fulminant pancreatitis is associated with significant morbidity and mortality. To examine the outcome of conservative and surgical treatment of this disorder, 36 patients who survived an initial episode were restudied after a mean of six years. Fifty three per cent had developed diabetes mellitus, half of whom r...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.6.843
更新日期:1992-06-01 00:00:00
abstract:BACKGROUND/AIMS:In 1998 the PPP2R1B gene encoding the A subunit of the serine/threonine protein phosphatase was identified as a putative tumour suppressor gene in lung and colon cancer in the chromosome region 11q22-24. The aim of the present study was to determine the type of alterations in primary rectal cancers as w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.47.2.268
更新日期:2000-08-01 00:00:00
abstract::To determine whether primary biliary cirrhosis differed in men and women we reviewed the presenting features and clinical course of 39 men and 191 women with primary biliary cirrhosis followed at this unit between 1970 and 1984. Age and severity of disease at time of diagnosis were similar in both groups. Pruritus was...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.27.11.1373
更新日期:1986-11-01 00:00:00
abstract::Synthetic 13-norleucine-motilin (13-nle-motilin), structural and biological analogue of the naturally-occurring duodenal polypeptide, motilin, is known to stimulate antral and duodenal motor activity in vitro, but delays gastric emptying in man. In this study the direct actions of the synthetic polypeptide on myoelect...
journal_title:Gut
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::Marked decreases in 24-hour Heidenhain pouch secretion, lasting from nine to 18 days, were observed in five mongrel bitches during periods of naturally occurring oestrus. Neither oophorectomy nor control celiotomy caused significant change in Heidenhain pouch secretion in bitches who were not experiencing oestrus. The...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.11.10.855
更新日期:1970-10-01 00:00:00
abstract:BACKGROUND:Epigenetic alterations accumulate in normal-appearing tissues of patients with cancer, producing an epigenetic field defect. Cross-sectional studies show that the degree of the defect may be associated with risk in some types of cancer, especially cancers associated with chronic inflammation. OBJECTIVE:To d...
journal_title:Gut
pub_type: 杂志文章,多中心研究
doi:10.1136/gutjnl-2014-307094
更新日期:2015-03-01 00:00:00
abstract::Serum gastrin levels have been studied in 70 patients with chronically reduced glomerular filtration rate (GFR) as estimated by 51Cr-EDTA clearance, creatinine, and beta2-microglobulin values. A strong dependence upon GFR was found, although the correlation between gastrin levels and GFR was not as high as that betwee...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.19.3.207
更新日期:1978-03-01 00:00:00
abstract:BACKGROUND AND AIMS:Although peroxisome proliferator activated receptor gamma (PPARgamma) agonists have been implicated in differentiation and growth inhibition of cancer cells, the potential therapeutic and chemopreventive effects on gastric cancer are poorly defined. We examined the in vitro and in vivo effects of PP...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2003.021105
更新日期:2004-03-01 00:00:00
abstract::Partial gastrectomy and intestinal resection significantly impaired glucose tolerance (p < 0.05) and significantly decreased the release of insulin by intravenous glucose (p < 0.05) or a meal of minced beef (p < 0.01). There was a significantly greater release of insulin one hour after oral glucose in the gastrectomy ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.13.11.871
更新日期:1972-11-01 00:00:00
abstract::The possibility that proliferative instability around a healing anastomosis promotes carcinogenesis was tested in 234 male Sprague-Dawley rats. Animals received the first of five weekly injections of azoxymethane (total dose 50 mg/kg) either immediately after transection of the descending colon or at 2, 4, 8, and 12 w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.28.7.808
更新日期:1987-07-01 00:00:00
abstract::An intensive histological search for Helicobacter pylori in gastric biopsy specimens has led to the detection of other spiral shaped bacteria in the human gastric mucosa. The clinical and morphological findings of 39 cases (0.25% of all gastric biopsies performed in the observation period) are reported for 34 patients...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.32.2.137
更新日期:1991-02-01 00:00:00
abstract:BACKGROUND AND AIMS:Emerging evidence suggests that highly treatment-resistant tumour-initiating cells (TICs) play a central role in the pathogenesis of pancreatic cancer. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered to be a novel anticancer agent; however, recent studies have shown th...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2008.149039
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND AND AIM:The lymphatic network plays a major role in maintaining tissue fluid homoeostasis. Therefore several pathological conditions associated with oedema formation result in deficient lymphatic function. However, the role of the lymphatic system in the pathogenesis of ascites and oedema formation in cirrho...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2011-300703
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:The number of colonoscopies required to reach competency is not well established. The primary aim of this study was to determine the number of colonoscopies trainees need to perform to attain competency, defined by a caecal intubation rate (CIR) ≥90%. As competency depends on completion, we also investigated ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2013-305973
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:Watery stools are equated with rapid and hard stools with slow intestinal transit; however, the relation between stool form and transit through specific regions of the gut is not clear cut. In addition, more information is needed on interindividual variability of these measurements. AIM:To examine the relat...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.39.1.109
更新日期:1996-07-01 00:00:00
abstract::Two patients who developed biochemical and histological evidence of hepatitis while taking the anti-anginal drug perhexiline maleate are described. The pathological changes were those of mild to moderate fatty change together with a hepatitis which resembled alcoholic hepatitis, including in one patient the presence o...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.20.3.186
更新日期:1979-03-01 00:00:00
abstract:BACKGROUND AND AIMS:Both bile salts and glutathione participate in the generation of canalicular bile flow. In this work, we have investigated the effect of different bile salts on hepatic glutathione metabolism. METHODS:Using the isolated and perfused rat liver, we studied hepatic glutathione content, and metabolism ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.50.5.701
更新日期:2002-05-01 00:00:00
abstract::A fifth instance of Crohn's disease in identical twins is reported. The evidence for genetic expression of the disease is considered and the possible mechanisms involved are reviewed. ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.10.12.994
更新日期:1969-12-01 00:00:00
abstract::Motilin is a hormonal peptide found in the duodenum and jejunum which potently influences gastrointestinal tract motility. Its role in human physiology is not yet established. After a standard hospital lunch the plasma concentration of motilin showed a small, transient, but significant rise in 28 healthy subjects. Ind...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.20.2.102
更新日期:1979-02-01 00:00:00
abstract:OBJECTIVE:Chronic pancreatitis is a progressive inflammatory disorder of the pancreas characterised by permanent destruction of acinar cells. Mutations in the chymotrypsinogen C (CTRC) gene have been linked to the development of chronic pancreatitis. The aim of the present study was to explore whether CTRC mutants indu...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2009.198903
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND:A high prevalence of osteoporosis is reported in Crohn's disease. The pathogenesis is not completely understood but is probably multifactorial. Longstanding Crohn's disease is associated with a deficiency of fat soluble vitamins, among them vitamin K. Vitamin K is a cofactor in the carboxylation of osteocalc...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.48.4.473
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND AND AIMS:Glucagon-like peptide 2 (GLP-2) may improve intestinal absorption in short bowel syndrome (SBS) patients with an end jejunostomy. Teduglutide (ALX-0600), a dipeptidyl peptidase IV resistant GLP-2 analogue, prolongs the intestinotrophic properties of GLP-2 in animal models. The safety and effect of t...
journal_title:Gut
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1136/gut.2004.061440
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:In hereditary pancreatitis mutations of exons 2 (N21I) and 3 (R117H) of the cationic trypsinogen gene have been described. AIMS:To investigate whether the same mutations can also be found in patients with chronic alcoholic pancreatitis. METHODS:Leucocyte DNA was prepared from 23 patients with chronic alcoh...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.44.3.413
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVE:Pancreatic ductal adenocarcinoma (PDAC) is a therapy recalcitrant disease with the worst survival rate of common solid tumours. Preclinical models that accurately reflect the genetic and biological diversity of PDAC will be important for delineating features of tumour biology and therapeutic vulnerabilities. ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2016-313133
更新日期:2018-03-01 00:00:00
abstract::A group of 80 patients with diseases of the small intestine were given a standardized psychiatric assessment every time they attended the outpatients' department over a period of a year. Forty-six patients with idiopathic steatorrhoea, 23 patients with Crohn's disease, and 11 patients with alactasia were interviewed o...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.11.6.459
更新日期:1970-06-01 00:00:00
abstract::Three cases are presented of lymphocytic colitis with chronic diarrhoea concurrent with longterm use of Cyclo 3 Fort, a phlebotonic drug used in France. The histological and immunopathological features of lymphocytic colitis are described. We show that lymphocytic colitis is drug induced, particularly in one patient w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.35.3.426
更新日期:1994-03-01 00:00:00
abstract:OBJECTIVE:Eosinophilic oesophagitis (EoO) is a clinicopathological condition defined by proton pump inhibitor-refractory oesophageal symptoms combined with oesophageal eosinophilia. The pharmacodynamic effect of mepolizumab (a humanised anti-interleukin-5 monoclonal antibody) in EoO was evaluated. METHODS:Eleven adult...
journal_title:Gut
pub_type: 杂志文章,随机对照试验
doi:10.1136/gut.2009.178558
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Several cases of acute colitis induced by non-steroidal anti-inflammatory drugs (NSAIDs) have been reported but the general role of recent NSAID intake as a risk factor for acute diarrhoea has not been studied to date. The aim of our study was to determine whether the risk of acute diarrhoea is incr...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.2.260
更新日期:2003-02-01 00:00:00
abstract::Cirrhosis is associated with the development of a hyperdynamic circulation, which is secondary to the presence of systemic vasodilatation. Several mechanisms have been postulated to be involved in the development of systemic vasodilatation, including increased synthesis of nitric oxide, hyperglucagonaemia, increased c...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.2004.056556
更新日期:2005-12-01 00:00:00
abstract::The effects of drugs which change the bile-salt-independent fraction of bile flow on Na(+)K(a+) and Mg(2+) activated ATPases were studied in membrane fractions rich in bile canaliculi. The administration of phenobarbital caused no induction of these enzymes which could explain the increased bile flow observed in the r...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.13.11.920
更新日期:1972-11-01 00:00:00