Abstract：
:The Drosophila mei-S332 gene acts to maintain sister-chromatid cohesion before anaphase II of meiosis in both males and females. By isolating and analyzing seven new alleles and a deficiency uncovering the mei-S332 gene we have demonstrated that the onset of the requirement for mei-S332 is not until late anaphase I. All of our alleles result primarily in equational (meiosis II) nondisjunction with low amounts of reductional (meiosis I) nondisjunction. Cytological analysis revealed that sister chromatids frequently separate in late anaphase I in these mutants. Since the sister chromatids remain associated until late in the first division, chromosomes segregate normally during meiosis I, and the genetic consequences of premature sister-chromatid dissociation are seen as nondisjunction in meiosis II. The late onset of mei-S332 action demonstrated by the mutations was not a consequence of residual gene function because two strong, and possibly null, alleles give predominantly equational nondisjunction both as homozygotes and in trans to a deficiency. mei-S332 is not required until after metaphase I, when the kinetochore differentiates from a single hemispherical kinetochore jointly organized by the sister chromatids into two distinct sister kinetochores. Therefore, we propose that the mei-S322 product acts to hold the doubled kinetochore together until anaphase II. All of the alleles are fully viable when in trans to a deficiency, thus mei-S332 is not essential for mitosis. Four of the alleles show an unexpected sex specificity.
journal_name
Geneticsjournal_title
Geneticsauthors
Kerrebrock AW,Miyazaki WY,Birnby D,Orr-Weaver TLkeywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
827-41issue
4eissn
0016-6731issn
1943-2631journal_volume
130pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The Saccharomyces cerevisiae SEN1 gene codes for a nuclear-localized superfamily I helicase. SEN1 is an ortholog of human SETX (senataxin), which has been implicated in the neurological disorders ataxia-ocular apraxia type 2 and juvenile amyotrophic lateral sclerosis. Pleiotropic phenotypes conferred by sen1 mutations...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.110031
更新日期：2010-01-01 00:00:00
abstract：:The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chrom...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.034801
更新日期：2004-10-01 00:00:00
abstract：:Nucleosome-depleted regions (NDRs) are present immediately adjacent to the transcription start site in most eukaryotic promoters. Here we show that NDRs in the upstream promoter region can profoundly affect gene regulation. Chromatin at the yeast HO promoter is highly repressive and numerous coactivators are required ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.183715
更新日期：2016-02-01 00:00:00
abstract：:Spt3 of Saccharomyces cerevisiae is required for the normal transcription of many genes in vivo. Past studies have shown that Spt3 is required for both mating and sporulation, two events that initiate when cells are at G(1)/START. We now show that Spt3 is needed for two other events that begin at G(1)/START, diploid f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:An analysis of mortality is undertaken in two breeds of pigs: Danish Landrace and Yorkshire. Zero-inflated and standard versions of hierarchical Poisson, binomial, and negative binomial Bayesian models were fitted using Markov chain Monte Carlo (MCMC). The objectives of the study were to investigate whether there is s...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.110759
更新日期：2010-01-01 00:00:00
abstract：:Doubly uniparental inheritance (DUI) provides an intriguing system for addressing aspects of molecular evolution and intermolecular recombination of mitochondrial DNA. For this reason, a large sequence analysis has been performed on Tapes philippinarum (Bivalvia, Veneridae), which has mitochondrial DNA heteroplasmy th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:The Aspergillus nidulans rcoADelta mutant exhibits growth and developmental defects. We show that the rcoADelta mutant lacks cleistothecia and is self-sterile. In crosses with wild-type strains, rcoADelta nuclei do not contribute to the cleistothecial walls. Furthermore, sexual development resulting from veA overexpre...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.062893
更新日期：2006-11-01 00:00:00
abstract：:A reversion study of EMS- or ICR170-induced ascospore color mutants in Ascobolus immersus is reported. Twenty-three new intragenic suppressors were isolated within the b2 locus. These are localized within three distinct groups on a genetic fine-structure map containing 21 identifiable sites. The pattern of reversion i...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
abstract：:The double-strand break repair models for homologous recombination propose that a double-strand break in a duplex DNA segment is repaired by gene conversion copying a homologous DNA segment. This is a type of conservative recombination, or two-progeny recombination, which generates two duplex DNA segments from two dup...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The heterotrimeric G protein Gq regulates neuronal activity through distinct downstream effector pathways. In addition to the canonical Gq effector phospholipase Cβ, the small GTPase Rho was recently identified as a conserved effector of Gq. To identify additional molecules important for Gq signaling in neurons, we pe...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300977
更新日期：2018-06-01 00:00:00
abstract：:We demonstrate that recent data from human males are consistent with constant interference levels among chromosomes under the two-pathway model, whereas inappropriately fitting shape parameters of Gamma distributions to immunofluorescent interfoci distances observed on finite chromosomes generates false interpretation...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.103853
更新日期：2009-09-01 00:00:00
abstract：:Orientation disruptor (ord), a meiotic mutant that is recombination defective in females and disjunction defective in males and females, has been analyzed using serial section electron and light microscopy. From analysis of primary spermatocytes we have confirmed that ord males are defective in some aspect of the mech...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
abstract：:We have found that temperature can have a striking effect upon protein export in Escherichia coli, suggesting that there is a cold-sensitive step in the protein export pathway. Cs mutations comprise the largest class of mutations affecting the membrane-localized Sec proteins SecD, SecE, SecF and SecY. Although some of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Key steps of essential metabolic pathways are housed in plant peroxisomes. We conducted a microscopy-based screen for anomalous distribution of peroxisomally targeted fluorescence in Arabidopsis thaliana This screen uncovered 34 novel alleles in 15 genes affecting oil body mobilization, fatty acid β-oxidation, the gly...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.193169
更新日期：2016-11-01 00:00:00
abstract：:An analysis of the effects of spontaneous mutations affecting age-specific mortality was conducted using 29 lines of Drosophila melanogaster that had accumulated spontaneous mutations for 19 generations. Divergence among the lines was used to estimate the mutational variance for weekly mortality rates and the covarian...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Two independent isolates of Saccharomyces cerevisiae lacking hexokinase activity (EC 2.7.1.1) are described. Both mutant strains grow on glucose but are unable to grow on fructose, and contain two mutant genes hxk1 and hxk2 each. The mutations are recessive and noncomplementing. Genetic analysis suggests that these tw...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
abstract：:For multiallelic loci, standard measures of linkage disequilibrium provide an incomplete description of the correlation of variation at two loci, especially when there are different numbers of alleles at the two loci. We have developed a complementary pair of conditional asymmetric linkage disequilibrium (ALD) measure...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.165266
更新日期：2014-09-01 00:00:00
abstract：:The allozymic variation at at the esterase-6 locus examined in fourteen samples of natural populations of Drosophila simulans collected in two different localities. The samples were collected from different species of fruits in the two localities and from banana baits located some meters apart from the fruits. The ana...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:We previously isolated a pollen factor, ui6.1, which encodes a Cullin1 protein (CUL1) that functions in unilateral interspecific incompatibility (UI) in Solanum. Here we show that CUL1 is also required for pollen function in self-incompatibility (SI). We used RNA interference (RNAi) to reduce CUL1 expression in pollen...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.158279
更新日期：2014-02-01 00:00:00
abstract：:Adaptive introgression is common in nature and can be driven by selection acting on multiple, linked genes. We explore the effects of polygenic selection on introgression under the infinitesimal model with linkage. This model assumes that the introgressing block has an effectively infinite number of loci, each with an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301018
更新日期：2018-08-01 00:00:00
abstract：:Accumulation of RNA was measured in adult males of two genotypes: car bb/Ybb- and car bb/YbbSuVar-5. The two genotypes have similar amounts of rDNA, which is reduced in comparison to wild type (CLARK, STRAUSBAUGH and KIEFER 1977). Although genotypically bobbed, car bb/YbbSuVar-5 flies have a wild-type phenotype; car b...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:The Drosophila gene exuperantia (exu) is a maternal effect gene which is needed for proper localization of the bcd RNA during the formation of oocytes. We have extended the characterization of the exu phenotype and find that the gene functions in the male as well as the female germline. Six of seven exu alleles are ma...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The homeotic genes of the Drosophila bithorax complex are controlled by a large cis-regulatory region that ensures their segmentally restricted pattern of expression. A deletion that removes the Frontabdominal-7 cis-regulatory region (Fab-7') dominantly transforms parasegment 11 into parasegment 12. Previous studies s...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:The RNA polymerase II (Pol-II) holoenzyme, responsible for messenger RNA production, typically consists of 10-12 subunits. Our laboratory previously demonstrated that maternally deposited, long, noncoding, template RNAs are essential for programmed genome rearrangements in the ciliate Oxytricha trifallax. Here we show...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.163279
更新日期：2014-07-01 00:00:00
abstract：:High-density genotyping of single-nucleotide polymorphisms (SNPs) enables detection of quantitative trait loci (QTL) by linkage disequilibrium (LD) mapping using LD between markers and QTL and the subsequent use of this information for marker-assisted selection (MAS). The success of LD mapping and MAS depends on the e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.082206
更新日期：2007-12-01 00:00:00
abstract：:Many aspects of plant biology depend on the ubiquitin proteasome system for degradation of regulatory proteins. Ubiquitin E3 ligases confer substrate specificity in this pathway, and SCF-type ligases comprise a major class of E3s. SCF ligases have four subunits: SKP1, CUL1, RBX1, and an F-box protein for substrate rec...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.097675
更新日期：2009-03-01 00:00:00
abstract：:Aggression is a complex social behavior that is widespread in nature. To date, only a limited number of genes that affect aggression have been identified, in large part because the complexity of the phenotype makes screening difficult and time-consuming regardless of the species that is studied. We discovered that agg...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300292
更新日期：2018-01-01 00:00:00
abstract：:Laboratory crosses between wild strains of the two European house mouse subspecies Mus musculus domesticus (2n = 34) and M. m. musculus (2n = 40) were performed to analyze the selective processes involved in the non-introgression of centromeric regions of Robertsonian (Rb) fusions in the Danish hybrid zone. The chromo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:A unique aspect of meiosis is the segregation of homologous chromosomes at the meiosis I division. The pairing of homologous chromosomes is a critical aspect of meiotic prophase I that aids proper disjunction at anaphase I. We have used a site-specific recombination assay in Saccharomyces cerevisiae to examine allelic...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.050658
更新日期：2006-07-01 00:00:00
abstract：:At least six nuclear loci are responsible for the genetic control of malate dehydrogenase (L-malate: NAD oxidoreductase; EC 1.1.1.37; MDH) in coleoptiles of maize. Three independently segregating loci (Mdh1, Mdh2, Mdh3) govern the production of MDH isozymes resistant to inactivation by ascorbic acid and found largely ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
