Abstract：
:The RNA polymerase II (Pol-II) holoenzyme, responsible for messenger RNA production, typically consists of 10-12 subunits. Our laboratory previously demonstrated that maternally deposited, long, noncoding, template RNAs are essential for programmed genome rearrangements in the ciliate Oxytricha trifallax. Here we show that such RNAs are bidirectionally transcribed and transported to the zygotic nucleus. The gene encoding the second-largest subunit of Pol-II, Rpb2, has undergone gene duplication, and the two paralogs, Rpb2-a and -b, display different expression patterns. Immunoprecipitation of double-stranded RNAs identified an association with Rpb2-a. Through immunoprecipitation and mass spectrometry, we show that Rpb2-a in early zygotes appears surprisingly unassociated with other Pol II subunits. A partial loss of function of Rpb2-a leads to an increase in expression of transposons and other germline-limited satellite repeats. We propose that evolutionary divergence of the Rpb2 paralogs has led to acquisition of transcription-independent functions during sexual reproduction that may contribute to the negative regulation of germline gene expression.
journal_name
Geneticsjournal_title
Geneticsauthors
Khurana JS,Wang X,Chen X,Perlman DH,Landweber LFdoi
10.1534/genetics.114.163279subject
Has Abstractpub_date
2014-07-01 00:00:00pages
839-49issue
3eissn
0016-6731issn
1943-2631pii
genetics.114.163279journal_volume
197pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Dosage compensation refers to the equal expression of X-linked genes despite the difference in copy number between the two sexes. The male-specific lethal (MSL) complex is concentrated on the X chromosome in males. A gene expression assay for embryos was developed to examine the function of this complex. In mutant mal...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.036020
更新日期：2005-04-01 00:00:00
abstract：:Damaged DNA-binding proteins 1 and 2 (DDB1 and DDB2) are subunits of the damaged DNA-binding protein complex (DDB). DDB1 is also found in the same complex as DE-ETIOLATED 1 (DET1), a negative regulator of light-mediated responses in plants. Arabidopsis has two DDB1 homologs, DDB1A and DDB1B. ddb1a single mutants have ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.070359
更新日期：2007-05-01 00:00:00
abstract：:We showed that sufficient overexpression of the wild-type recF gene interfered with three normal cell functions: (1) UV induction of transcription from the LexA-protein-repressed sulA promoter, (2) UV resistance and (3) cell viability at 42 degrees. To show this, we altered a low-level overexpressing recF+ plasmid wit...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Biofilms are microbial communities that form on surfaces. They are the primary form of microbial growth in nature and can have detrimental impacts on human health. Some strains of the budding yeast Saccharomyces cerevisiae form colony biofilms, and there is substantial variation in colony architecture between biofilm-...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.142067
更新日期：2013-02-01 00:00:00
abstract：:The effect of nonhomologous DNA sequences at one or both sides of short genetic intervals on recombination within that interval was investigated, using an interplasmidic recombination system in Escherichia coli K-12. The recombining plasmids were derivatives of pBR322 and pACYC184, which share a 1330-nucleotide sequen...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:New rudimentary (r) mutants have been isolated following mutagenesis with ethyl methanesulfonate (r(LE)), ICR-170 (r(LI)) and X rays (r(LX)). From wing phenotype measurements on homoallelic females, it has been shown that the r(LE) mutant series includes several leaky alleles, as well as alleles that produce moderate ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:The genetic structure of green turtle (Chelonia mydas) rookeries located around the Australian coast was assessed by (1) comparing the structure found within and among geographic regions, (2) comparing microsatellite loci vs. restriction fragment length polymorphism analyses of anonymous single copy nuclear DNA (ascnD...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:CarD is the only reported prokaryotic protein showing structural and functional features typical of eukaryotic high-mobility group A transcription factors. In prokaryotes, proteins similar to CarD appear to be confined primarily to myxobacteria. In Myxococcus xanthus, CarD has been previously shown to act as a positiv...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.029207
更新日期：2004-08-01 00:00:00
abstract：:In many organisms, female and male meiosis display extensive sexual dimorphism in the temporal meiotic program, the number and location of recombination events, sex chromosome segregation, and checkpoint function. We show here that both meiotic prophase timing and germ-line apoptosis, one output of checkpoint signalin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.110338
更新日期：2010-03-01 00:00:00
abstract：:Divergence of the hyperthermophilic Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was assessed by analysis of complete genomic sequences of both species. The average nucleotide identity between the genomic sequences is 70-75% within ORFs. The P. furiosus genome (1.908 mbp) is 170 kbp larger than the P. horik...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:A long-standing prediction in evolutionary biology is that organisms experience a so-called "cost of complexity" manifested as a decreasing rate of adaptation in populations as organisms or selective environments become increasingly complex. This theory assumes the ubiquity of antagonistic pleiotropy, or trade-offs in...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302029
更新日期：2019-05-01 00:00:00
abstract：:Bacteriophage T4 DNA metabolism is largely insulated from that of its host, although some host functions assist in the repair of T4 DNA damage. Environmental factors sometimes affect survival and mutagenesis after ultraviolet (UV) irradiation of T4, and can affect mutagenesis in many organisms. We therefore tested the...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:A reversion study of EMS- or ICR170-induced ascospore color mutants in Ascobolus immersus is reported. Twenty-three new intragenic suppressors were isolated within the b2 locus. These are localized within three distinct groups on a genetic fine-structure map containing 21 identifiable sites. The pattern of reversion i...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
abstract：:Although maize is naturally an outcrossing organism, modern breeding utilizes highly inbred lines in controlled crosses to produce hybrids. The U.S. Department of Agriculture's reciprocal recurrent selection experiment between the Iowa Stiff Stalk Synthetic (BSSS) and the Iowa Corn Borer Synthetic No. 1 (BSCB1) popula...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.182410
更新日期：2015-11-01 00:00:00
abstract：:Methodologies for mapping of genes underlying quantitative traits have advanced considerably but have not been accompanied by a parallel development of new population structures. We present a novel population consisting of 50 introgression lines (ILs) originating from a cross between the green-fruited species Lycopers...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The possibility that fitness relationships associated with an inversion polymorphism in D. paulistorum were frequency dependent was investigated. Using allozymes of tetrazolium oxidase to mark inversions, the effects of genotype frequency, larval density, and culture conditions on fitness were assessed. The proportion...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:Lethal and sterility mutations were accumulated in a cage population which was initiated with lethal- and sterility-free second chromosomes of D. melanogaster. It took about 2,000 days for the frequencies of these genes to reach equilibrium levels, i.e., 18% lethal and 9% male-sterile chromosomes. Two other cage popul...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:We have characterized a Saccharomyces cerevisiae mutant strain that is hypersensitive to cyclosporin A (CsA) and FK506, immunosuppressants that inhibit calcineurin, a serine-threonine-specific phosphatase (PP2B). A single nuclear mutation, designated cev1 for calcineurin essential for viability, is responsible for the...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate ade6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G----T nucleotide change, that M26 stimulated recombination within ade6 but not at other distinct loci, and that M26 stimulated meiot...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:The consequences of a discontinuity in the migration rate and of a geographical barrier in the habitat are studied in a diffusion model of migration and selection. The treatment is restricted to a single diallelic locus in a monoecious population in the absence of mutation and random drift. It is supposed further that...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:We explicitly solve and analyze a series of deterministic continent-island models to delimit the effects of pollen and seed migration on cytonuclear frequencies and disequilibria in random-mating, mixed-mating and self-fertilized populations. Given the critical assumption of maternal cytoplasmic inheritance, five majo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:Position effect variegation results from chromosome rearrangements which translocate euchromatic genes close to the heterochromatin. The euchromatin-heterochromatin association is responsible for the inactivation of these genes in some cell clones. In Drosophila melanogaster the Y chromosome, which is entirely heteroc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:We test models for the evolution of helical regions of RNA sequences, where the base pairing constraint leads to correlated compensatory substitutions occurring on either side of the pair. These models are of three types: 6-state models include only the four Watson-Crick pairs plus GU and UG; 7-state models include a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Selection of suppressor mutations that correct growth defects caused by substitutions in an RNA or protein can reveal functionally important molecular structures and interactions in living cells. This approach is particularly useful for the study of complex biological pathways involving many macromolecules, such as pr...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.301922
更新日期：2019-05-01 00:00:00
abstract：:Many arthropod species are infected with maternally inherited endosymbionts that induce a shift in the sex ratio of their hosts by feminizing or killing males (cytoplasmic sex-ratio distorters, or SRDs). These endosymbionts can have profound impacts on evolutionary processes of their hosts. Here, I derive analytical e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.120014
更新日期：2010-09-01 00:00:00
abstract：:Aggression is a complex social behavior that is widespread in nature. To date, only a limited number of genes that affect aggression have been identified, in large part because the complexity of the phenotype makes screening difficult and time-consuming regardless of the species that is studied. We discovered that agg...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300292
更新日期：2018-01-01 00:00:00
abstract：:Two Podospora mutants carrying mutations modE and modF were persumed to be quiescent defective, because, when grown under glucose limitation, they differed from the wild-type strain in an excess of dry weight production and a reduction of cell survival. New insight on the action of modE and modF mutations was provided...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Positive selection on a new mutant allele can increase the frequencies of closely linked alleles (through hitchhiking), as well as create linkage disequilibrium between them. Because this disequilibrium is induced by the selected allele, one may be able to identify loci under selection by measuring the influence of a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:The distribution of fitness effects of new mutations (DFE) is important for addressing several questions in genetics, including the nature of quantitative variation and the evolutionary fate of small populations. Properties of the DFE can be inferred by comparing the distributions of the frequencies of segregating nuc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.080663
更新日期：2007-12-01 00:00:00
abstract：:A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites we...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.075416
更新日期：2007-12-01 00:00:00
