vph6 mutants of Saccharomyces cerevisiae require calcineurin for growth and are defective in vacuolar H(+)-ATPase assembly.

Abstract:

:We have characterized a Saccharomyces cerevisiae mutant strain that is hypersensitive to cyclosporin A (CsA) and FK506, immunosuppressants that inhibit calcineurin, a serine-threonine-specific phosphatase (PP2B). A single nuclear mutation, designated cev1 for calcineurin essential for viability, is responsible for the CsA-FK506-sensitive phenotype. The peptidyl-prolyl cis-trans isomerases cyclophilin A and FKBP12, respectively, mediate CsA and FK506 toxicity in the cev1 mutant strain. We demonstrate that cev1 is an allele of the VPH6 gene and that vph6 mutant strains fail to assemble the vacuolar H(+)-ATPase (V-ATPase). The VPH6 gene was mapped on chromosome VIII and is predicted to encode a 181-amino acid (21 kD) protein with no identity to other known proteins. We find that calcineurin is essential for viability in many mutant strains with defects in V-ATPase function or vacuolar acidification. In addition, we find that calcineurin modulates extracellular acidification in response to glucose, which we propose occurs via calcineurin regulation of the plasma membrane H(+)-ATPase PMA1. Taken together, our findings suggest calcineurin plays a general role in the regulation of cation transport and homeostasis.

journal_name

Genetics

journal_title

Genetics

authors

Hemenway CS,Dolinski K,Cardenas ME,Hiller MA,Jones EW,Heitman J

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

833-44

issue

3

eissn

0016-6731

issn

1943-2631

journal_volume

141

pub_type

杂志文章

相关文献

GENETICS文献大全
  • Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley.

    abstract::Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (H...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.114.165613

    authors: Pankin A,Campoli C,Dong X,Kilian B,Sharma R,Himmelbach A,Saini R,Davis SJ,Stein N,Schneeberger K,von Korff M

    更新日期:2014-09-01 00:00:00

  • Intragenic Suppression at the b2 Locus in ASCOBOLUS IMMERSUS. II. Characteristics of the Mutations in Groups A and E.

    abstract::The present report is a study of 60 intragenic suppressors located in the A or E group of the b2 spore-color locus of Ascobolus immersus. The frameshift nature of the suppressors was shown by 19 combinations of plus and minus suppressor mutations. The location of the mutation sites on the genetic map of group A was de...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Leblon G

    更新日期:1979-08-01 00:00:00

  • An analysis of frequencies of chromosome configurations in wheat and wheat hybrids.

    abstract::Presynaptic association of homologous chromosomes is a prerequisite to the sequence of events that lead to chiasma formation. This association of homologous chromosomes, as entire units, occurs with probability a, and chiasma formation occurs independently in opposite chromosome arms with probability c. a and c have b...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Driscoll CJ,Bielig LM,Darvey NL

    更新日期:1979-04-01 00:00:00

  • The maize unstable factor for orange1 is a dominant epigenetic modifier of a tissue specifically silent allele of pericarp color1.

    abstract::We have characterized Unstable factor for orange1 (Ufo1), a dominant, allele-specific modifier of expression of the maize pericarp color1 (p1) gene. The p1 gene encodes an Myb-homologous transcriptional activator of genes required for biosynthesis of red phlobaphene pigments. The P1-wr allele specifies colorless kerne...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Chopra S,Cocciolone SM,Bushman S,Sangar V,McMullen MD,Peterson T

    更新日期:2003-03-01 00:00:00

  • OrthoList 2: A New Comparative Genomic Analysis of Human and Caenorhabditis elegans Genes.

    abstract::OrthoList, a compendium of Caenorhabditis elegans genes with human orthologs compiled in 2011 by a meta-analysis of four orthology-prediction methods, has been a popular tool for identifying conserved genes for research into biological and disease mechanisms. However, the efficacy of orthology prediction depends on th...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.118.301307

    authors: Kim W,Underwood RS,Greenwald I,Shaye DD

    更新日期:2018-10-01 00:00:00

  • The evolution of age-specific mortality rates in Drosophila melanogaster: genetic divergence among unselected lines.

    abstract::Age-specific effects of spontaneous mutations on mortality rates in Drosophila are inferred from three large demographic experiments. Data were collected from inbred lines that were allowed to accumulate spontaneous mutations for 10, 19, and 47 generations. Estimates of age-specific mutational variance for mortality w...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Pletcher SD,Houle D,Curtsinger JW

    更新日期:1999-10-01 00:00:00

  • Functional characterization of Drosophila microRNAs by a novel in vivo library.

    abstract::Animal microRNAs (miRNA) are implicated in the control of nearly all cellular functions. Due to high sequence redundancy within the miRNA gene pool, loss of most of these 21- to 24-bp long RNAs individually does not cause a phenotype. Thus, only very few miRNAs have been associated with clear functional roles. We cons...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.112.145383

    authors: Schertel C,Rutishauser T,Förstemann K,Basler K

    更新日期:2012-12-01 00:00:00

  • Satellite DNA from the Y chromosome of the malaria vector Anopheles gambiae.

    abstract::Satellite DNA is an enigmatic component of genomic DNA with unclear function that has been regarded as "junk." Yet, persistence of these tandem highly repetitive sequences in heterochromatic regions of most eukaryotic chromosomes attests to their importance in the genome. We explored the Anopheles gambiae genome for t...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.034264

    authors: Krzywinski J,Sangaré D,Besansky NJ

    更新日期:2005-01-01 00:00:00

  • Meiotic chromosome dynamics dependent upon the rec8(+), rec10(+) and rec11(+) genes of the fission yeast Schizosaccharomyces pombe.

    abstract::During meiosis homologous chromosomes replicate once, pair, experience recombination, and undergo two rounds of segregation to produce haploid meiotic products. The rec8(+), rec10(+), and rec11(+) genes of the fission yeast Schizosaccharomyces pombe exhibit similar specificities for meiotic recombination and rec8(+) i...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Krawchuk MD,DeVeaux LC,Wahls WP

    更新日期:1999-09-01 00:00:00

  • On the effect of fluctuating recombination rates on the decorrelation of gene histories in the human genome.

    abstract::We show how to incorporate fluctuations of the recombination rate along the chromosome into standard gene-genealogical models for the decorrelation of gene histories. This enables us to determine how small-scale fluctuations (Poissonian hot-spot model) and large-scale variations (Kong et al. 2002) of the recombination...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.103.018002

    authors: Eriksson A,Mehlig B

    更新日期:2005-02-01 00:00:00

  • The sex-ratio trait in Drosophila simulans: genetic analysis of distortion and suppression.

    abstract::The sex-ratio trait described in several Drosophila species is a type of naturally occurring X-linked meiotic drive that causes males bearing a sex-ratio X chromosome to produce progenies with a large excess of females. We have previously reported the occurrence of sex-ratio X chromosomes in Drosophila simulans. In th...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Cazemajor M,Landré C,Montchamp-Moreau C

    更新日期:1997-10-01 00:00:00

  • Patterns of Genetic and Phenotypic Suppression of lys2 Mutations in the Yeast SACCHAROMYCES CEREVISIAE.

    abstract::A total of 358 lys2 mutants of Saccharomyces cerevisiae have been characterized for suppressibility by the following suppressors: UAA and UAG suppressors that insert tyrosine, serine or leucine; a putative UGA suppressor; an omnipotent suppressor SUP46; and a frameshift suppressor SUF1-1. In addition, the lys2 mutants...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Chattoo BB,Palmer E,Ono B,Sherman F

    更新日期:1979-09-01 00:00:00

  • Evolution of mouse T-box genes by tandem duplication and cluster dispersion.

    abstract::The T-box genes comprise an ancient family of putative transcription factors conserved across species as divergent as Mus musculus and Caenorhabditis elegans. All T-box gene products are characterized by a novel 174-186-amino acid DNA binding domain called the T-box that was first discovered in the polypeptide product...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Agulnik SI,Garvey N,Hancock S,Ruvinsky I,Chapman DL,Agulnik I,Bollag R,Papaioannou V,Silver LM

    更新日期:1996-09-01 00:00:00

  • FLAGS: A Flexible and Adaptive Association Test for Gene Sets Using Summary Statistics.

    abstract::Genome-wide association studies (GWAS) have been widely used for identifying common variants associated with complex diseases. Despite remarkable success in uncovering many risk variants and providing novel insights into disease biology, genetic variants identified to date fail to explain the vast majority of the heri...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.115.185009

    authors: Huang J,Wang K,Wei P,Liu X,Liu X,Tan K,Boerwinkle E,Potash JB,Han S

    更新日期:2016-03-01 00:00:00

  • An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster.

    abstract::We developed a screening approach that utilizes an inverse polymerase chain reaction (PCR) to detect P element insertions in or near previously cloned genes in Drosophila melanogaster. We used this approach in a large scale genetic screen in which P elements were mobilized from sites on the X chromosome to new autosom...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Dalby B,Pereira AJ,Goldstein LS

    更新日期:1995-02-01 00:00:00

  • A genome-scan method to identify selected loci appropriate for both dominant and codominant markers: a Bayesian perspective.

    abstract::Identifying loci under natural selection from genomic surveys is of great interest in different research areas. Commonly used methods to separate neutral effects from adaptive effects are based on locus-specific population differentiation coefficients to identify outliers. Here we extend such an approach to estimate d...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.108.092221

    authors: Foll M,Gaggiotti O

    更新日期:2008-10-01 00:00:00

  • Wingless Signaling: A Genetic Journey from Morphogenesis to Metastasis.

    abstract::This FlyBook chapter summarizes the history and the current state of our understanding of the Wingless signaling pathway. Wingless, the fly homolog of the mammalian Wnt oncoproteins, plays a central role in pattern generation during development. Much of what we know about the pathway was learned from genetic and molec...

    journal_title:Genetics

    pub_type: 杂志文章,评审

    doi:10.1534/genetics.117.300157

    authors: Bejsovec A

    更新日期:2018-04-01 00:00:00

  • Spontaneous mutational correlations for life-history, morphological and behavioral characters in Caenorhabditis elegans.

    abstract::The pattern of mutational covariance among traits plays a central, but largely untested, role in many theories in evolutionary genetics. Here we estimate the pattern of phenotypic, environmental, and mutational correlations for a set of life-history, behavioral, and morphological traits using 67 self-fertilizing lines...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.040022

    authors: Estes S,Ajie BC,Lynch M,Phillips PC

    更新日期:2005-06-01 00:00:00

  • Evidence for local regulatory control of escape from imprinted X chromosome inactivation.

    abstract::X chromosome inactivation (XCI) is an epigenetic process that almost completely inactivates one of two X chromosomes in somatic cells of mammalian females. A few genes are known to escape XCI and the mechanism for this escape remains unclear. Here, using mouse trophoblast stem (TS) cells, we address whether particular...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.114.162800

    authors: Mugford JW,Starmer J,Williams RL Jr,Calabrese JM,Mieczkowski P,Yee D,Magnuson T

    更新日期:2014-06-01 00:00:00

  • Comparative analysis of position-effect variegation mutations in Drosophila melanogaster delineates the targets of modifiers.

    abstract::In Drosophila melanogaster, heterochromatin-induced silencing or position-effect variegation (PEV) of a reporter gene has provided insights into the properties of heterochromatin. Class I modifiers suppress PEV, and class II modifiers enhance PEV when the modifier gene is present in fewer than two doses. We have exami...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Sass GL,Henikoff S

    更新日期:1998-02-01 00:00:00

  • New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.

    abstract::I factors in Drosophila melanogaster are non-LTR retrotransposons that transpose at very high frequencies in the germ line of females resulting from crosses between reactive females (devoid of active I factors) and inducer males (containing active I factors). Constructs containing I factor ORF1 under the control of th...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Malinsky S,Bucheton A,Busseau I

    更新日期:2000-11-01 00:00:00

  • Conditions under which genome-wide association studies will be positively misleading.

    abstract::Genome-wide association mapping is a popular method for using natural variation within a species to generate a genotype-phenotype map. Statistical association between an allele at a locus and the trait in question is used as evidence that variation at the locus is responsible for variation of the trait. Indirect assoc...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.110.121665

    authors: Platt A,Vilhjálmsson BJ,Nordborg M

    更新日期:2010-11-01 00:00:00

  • Dominance of mutations affecting viability in Drosophila melanogaster.

    abstract::There have been several attempts to estimate the average dominance (ratio of heterozygous to homozygous effects) of spontaneous deleterious mutations in Drosophila melanogaster, but these have given inconsistent results. We investigated whether transposable element (TE) insertions have higher average dominance for egg...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Fry JD,Nuzhdin SV

    更新日期:2003-04-01 00:00:00

  • Major chromosomal rearrangements induced by T-DNA transformation in Arabidopsis.

    abstract::We show that major chromosomal rearrangements can occur upon T-DNA transformation of Arabidopsis thaliana. In the ACL4 line, two T-DNA insertion loci were found; one is a tandem T-DNA insert in a head-to-head orientation, and the other is a truncated insert with only the left part of the T-region. The four flanking DN...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Nacry P,Camilleri C,Courtial B,Caboche M,Bouchez D

    更新日期:1998-06-01 00:00:00

  • Cloning and analysis of the alternative oxidase gene of Neurospora crassa.

    abstract::Mitochondria of Neurospora crassa contain a cyanide-resistant alternative respiratory pathway in addition to the cytochrome pathway. The alternative oxidase is present only when electron flow through the cytochrome chain is restricted. Both genomic and cDNA copies for the alternative oxidase gene have been isolated an...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Li Q,Ritzel RG,McLean LL,McIntosh L,Ko T,Bertrand H,Nargang FE

    更新日期:1996-01-01 00:00:00

  • The nature of quantitative genetic variation in Drosophila. III. Mechanism of dosage compensation for sex-linked abdominal bristle polygenes.

    abstract::Seventeen lines, each homozygous for a different X chromosome but all with a common autosomal genetic blackground, were constructed and assayed for abdominal bristle number to determine whether dosage compensation operates for sex-linked genes affecting this character. --The regression coefficient of male mean on fema...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Frankham R

    更新日期:1977-01-01 00:00:00

  • DNA sequence evolution of the amylase multigene family in Drosophila pseudoobscura.

    abstract::The alpha-Amylase locus in Drosophila pseudoobscura is a multigene family of one, two or three copies on the third chromosome. The nucleotide sequences of the three Amylase genes from a single chromosome of D. pseudoobscura are presented. The three Amylase genes differ at about 0.5% of their nucleotides. Each gene has...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Brown CJ,Aquadro CF,Anderson WW

    更新日期:1990-09-01 00:00:00

  • Adaptation at specific loci. IV. Differential mating success among glycolytic allozyme genotypes of Colias butterflies.

    abstract::Male mating success as a function of genotype is an important fitness component. It can be studied in wild populations, in species for which a given group of progeny has exactly one father, by determining genotypes of wildcaught mothers and of sufficient numbers of their progeny. Here, we study male mating success as ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Watt WB,Carter PA,Blower SM

    更新日期:1985-01-01 00:00:00

  • Multiplicative genotype-environment interaction as a cause of reversed response to directional selection.

    abstract::In experiments with directional selection on a quantitative character a "reversed response" to selection is occasionally observed, when selection of individuals for a higher (lower) value of the character results in a lower (higher) value of the character among their offspring. A sudden change in environments or rando...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Gimelfarb A

    更新日期:1986-09-01 00:00:00

  • Genetic linkage maps of the red flour beetle, Tribolium castaneum, based on bacterial artificial chromosomes and expressed sequence tags.

    abstract::A genetic linkage map was constructed in a backcross family of the red flour beetle, Tribolium castaneum, based largely on sequences from bacterial artificial chromosome (BAC) ends and untranslated regions from random cDNA's. In most cases, dimorphisms were detected using heteroduplex or single-strand conformational p...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.032227

    authors: Lorenzen MD,Doyungan Z,Savard J,Snow K,Crumly LR,Shippy TD,Stuart JJ,Brown SJ,Beeman RW

    更新日期:2005-06-01 00:00:00