Abstract:
:Key steps of essential metabolic pathways are housed in plant peroxisomes. We conducted a microscopy-based screen for anomalous distribution of peroxisomally targeted fluorescence in Arabidopsis thaliana This screen uncovered 34 novel alleles in 15 genes affecting oil body mobilization, fatty acid β-oxidation, the glyoxylate cycle, peroxisome fission, and pexophagy. Partial loss-of-function of lipid-mobilization enzymes conferred peroxisomes clustered around retained oil bodies without other notable defects, suggesting that this microscopy-based approach was sensitive to minor perturbations, and that fatty acid β-oxidation rates in wild type are higher than required for normal growth. We recovered three mutants defective in PECTIN METHYLESTERASE31, revealing an unanticipated role in lipid mobilization for this cytosolic enzyme. Whereas mutations reducing fatty acid import had peroxisomes of wild-type size, mutations impairing fatty acid β-oxidation displayed enlarged peroxisomes, possibly caused by excess fatty acid β-oxidation intermediates in the peroxisome. Several fatty acid β-oxidation mutants also displayed defects in peroxisomal matrix protein import. Impairing fatty acid import reduced the large size of peroxisomes in a mutant defective in the PEROXISOMAL NAD+ TRANSPORTER (PXN), supporting the hypothesis that fatty acid accumulation causes pxn peroxisome enlargement. The diverse mutants isolated in this screen will aid future investigations of the roles of β-oxidation and peroxisomal cofactor homeostasis in plant development.
journal_name
Geneticsjournal_title
Geneticsauthors
Rinaldi MA,Patel AB,Park J,Lee K,Strader LC,Bartel Bdoi
10.1534/genetics.116.193169subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1089-1115issue
3eissn
0016-6731issn
1943-2631pii
genetics.116.193169journal_volume
204pub_type
杂志文章相关文献
GENETICS文献大全abstract::The consequences of a discontinuity in the migration rate and of a geographical barrier in the habitat are studied in a diffusion model of migration and selection. The treatment is restricted to a single diallelic locus in a monoecious population in the absence of mutation and random drift. It is supposed further that...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::Over one-third of human genome sequence is a product of non-LTR retrotransposition. The retrotransposon that currently drives this process in humans is the highly abundant LINE-1 (L1) element. Despite the ubiquitous nature of L1's in mammals, we still lack a complete mechanistic understanding of the L1 replication cyc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.096636
更新日期:2009-01-01 00:00:00
abstract::Aimed at investigating the recovery of a specific mutant allele of the mating type locus (MAT) by switching a defective MAT allele, these experiments provide information bearing on several models proposed for MAT interconversion in bakers yeast, Saccharomyces cerevisiae. Hybrids between heterothallic (ho) cells carryi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-07-01 00:00:00
abstract::Translocation induced in the immature oocyte, in meiotic prophase, affects division I orientation and segregation, the usual result being that the two halves of translocations are directed to opposite poles. Since interchange is usually (if not exclusively) between chromatids, this is to be expected from the creation ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-09-01 00:00:00
abstract::Neutrality tests based on the frequency spectrum (e.g., Tajima's D or Fu and Li's F) are commonly used by population geneticists as routine tests to assess the goodness-of-fit of the standard neutral model on their data sets. Here, I show that these neutrality tests are specific instances of a general model that encom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.104042
更新日期:2009-09-01 00:00:00
abstract::Progress in understanding the evolution of variation at the MHC has been slowed by an inability to assess the relative roles of mutation vs. intragenic recombination in contributing to observed polymorphism. Recent theoretical advances now permit a quantitative treatment of the problem, with the result that the amount...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::Synthetics play an important role in quantitative genetic research and plant breeding, but few studies have investigated the application of genomic prediction (GP) to these populations. Synthetics are generated by intermating a small number of parents ([Formula: see text] and thereby possess unique genetic properties,...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.193243
更新日期:2017-01-01 00:00:00
abstract::The small heat-shock proteins have undergone a tremendous diversification in plants; whereas only a single small heat-shock protein is found in fungi and many animals, over 20 different small heat-shock proteins are found in higher plants. The small heat-shock proteins in plants have diversified in both sequence and c...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::A genotype probability index (GPI) is proposed to indicate the information content of genotype probabilities derived from a segregation analysis. Typically, some individuals are genotyped at a marker locus or a quantitative trait locus, and segregation analysis is used to make genotype inferences about ungenotyped rel...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::We investigated genetic polymorphism in the Caenorhabditis elegans srh and str chemoreceptor gene families, each of which consists of approximately 300 genes encoding seven-pass G-protein-coupled receptors. Almost one-third of the genes in each family are annotated as pseudogenes because of apparent functional defects...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.035329
更新日期:2005-04-01 00:00:00
abstract::The fine-scale pattern of correlated paternity was characterized within a population of the narrow-endemic model plant species, Centaurea corymbosa, using microsatellites and natural progeny arrays. We used classical approaches to assess correlated mating within sibships and developed a new method based on pairwise ki...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.027714
更新日期:2004-11-01 00:00:00
abstract::The specification of floral organ identity in the higher dicots depends on the function of a limited set of homeotic genes, many of them members of the MADS-box gene family. Two such genes, APETALA3 (AP3) and PISTILLATA (PI), are required for petal and stamen identity in Arabidopsis; their orthologs in Antirrhinum exh...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::In some strains of Saccharomyces cerevisiae, the induction of enzymes of the Leloir pathway, galactose fermentation and growth on galactose depend on mitochondrial function; mitochondrial dependence is elicited through the recessive allele imp1 of the nuclear gene IMP1. The genetic element IMP1 is not allelic to any o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Molecular heterogeneity in human breast cancer has challenged diagnosis, prognosis, and clinical treatment. It is well known that molecular subtypes of breast tumors are associated with significant differences in prognosis and survival. Assuming that the differences are attributed to subtype-specific pathways, we then...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.180125
更新日期:2015-12-01 00:00:00
abstract::The relationships between synonymous and nonsynonymous substitution rates and between synonymous rate and codon usage bias are important to our understanding of the roles of mutation and selection in the evolution of Drosophila genes. Previous studies used approximate estimation methods that ignore codon bias. In this...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::As one of the world's most important food crops, the potato (Solanum tuberosum L.) has spurred innovation in autotetraploid genetics, including in the use of SNP arrays to determine allele dosage at thousands of markers. By combining genotype and pedigree information with phenotype data for economically important trai...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300685
更新日期:2018-05-01 00:00:00
abstract::Regulation of cytoskeletal structure and dynamics is essential for multiple aspects of cellular behavior, yet there is much to learn about the molecular machinery underlying the coordination between the cytoskeleton and its effector systems. One group of proteins that regulate microtubule behavior and its interaction ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.115626
更新日期:2010-08-01 00:00:00
abstract::Advances in medicine, agriculture, and an understanding of evolution depend on resolving the genetic architecture of quantitative traits, which is challenging since variation for complex traits is caused by multiple interacting quantitative trait loci (QTL) with small and conditional effects. Here, we show that the ke...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::The percentage of crossovers is consistently higher in plants hypoploid for six B-A translocations when crossed as males than when crossed as females; in most instances, this excess of male crossing over exceeds that found in control crosses (involving normal chromosomes). Thus, there seems to be something about the h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Microsatellites are tandemly repeated short DNA sequences that are favored as molecular-genetic markers due to their high polymorphism index. Plant genomes characterized to date exhibit taxon-specific differences in frequency, genomic location, and motif structure of microsatellites, indicating that extant microsatell...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.054791
更新日期:2006-04-01 00:00:00
abstract::Fifty-eight isochromosomal lines sampled from two natural populations of Drosophila pseudoobscura in California and one from Bogota, Colombia, were examined using four-cutter restriction mapping. A 4.6-kb region of the xanthine dehydrogenase locus was probed and 66 of 135 restriction sites scored were polymorphic. Thi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::Triose phosphate isomerase (TPI) deficiency glycolytic enzymopathy is a progressive neurodegenerative condition that remains poorly understood. The disease is caused exclusively by specific missense mutations affecting the TPI protein and clinically features hemolytic anemia, adult-onset neurological impairment, degen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.087551
更新日期:2008-06-01 00:00:00
abstract::Rodent inbred line crosses are widely used to map genetic loci associated with complex traits. This approach has proven to be powerful for detecting quantitative trait loci (QTL); however, the resolution of QTL locations, typically approximately 20 cM, means that hundreds of genes are implicated as potential candidate...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.033993
更新日期:2005-03-01 00:00:00
abstract::The availability of the complete DNA sequence of the Chlamydomonas reinhardtii genome and advanced computational biology tools has allowed elucidation and study of the small ubiquitin-like modifier (SUMO) system in this unicellular photosynthetic alga and model eukaryotic cell system. SUMO is a member of a ubiquitin-l...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.089128
更新日期:2008-05-01 00:00:00
abstract::The fission yeast Schizosaccharomyces pombe does not form tripartite synaptonemal complexes during meiotic prophase, but axial core-like structures (linear elements). To probe the relationship between meiotic recombination and the structure, pairing, and segregation of meiotic chromosomes, we genetically and cytologic...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The Drosophila melanogaster Gart gene encodes three enzymatic activities in the pathway for purine de novo synthesis. Alternative processing of the primary transcript leads to the synthesis of two overlapping polypeptides. The coding sequence for both polypeptides is interrupted by an intron that contains a functional...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::Smg1 is a key component of nonsense-mediated decay (NMD) in Caenorhabditis elegans and mammals. Here we report that two nonsense alleles of the ortholog of Smg1 do not affect NMD in Drosophila melanogaster. ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.045674
更新日期:2005-09-01 00:00:00
abstract::The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051144
更新日期:2006-02-01 00:00:00
abstract::The fixation of a beneficial allele in a population leaves a well-characterized signature in patterns of nucleotide variation at linked sites. This signature can be used to estimate the time since fixation from patterns of polymorphism in extant individuals. I introduce a method to assess the support in polymorphism d...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::We describe here a family of P elements that we refer to as type I repressors. These elements are identified by their repressor functions and their lack of any deletion within the first two-thirds of the canonical P sequence. Elements belonging to this repressor class were isolated from P strains and were made in vitr...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00