Abstract：
:The heterotrimeric G protein Gq regulates neuronal activity through distinct downstream effector pathways. In addition to the canonical Gq effector phospholipase Cβ, the small GTPase Rho was recently identified as a conserved effector of Gq. To identify additional molecules important for Gq signaling in neurons, we performed a forward genetic screen in the nematode Caenorhabditis elegans for suppressors of the hyperactivity and exaggerated waveform of an activated Gq mutant. We isolated two mutations affecting the MAP kinase scaffold protein KSR-1 and found that KSR-1 modulates locomotion downstream of, or in parallel to, the Gq-Rho pathway. Through epistasis experiments, we found that the core ERK MAPK cascade is required for Gq-Rho regulation of locomotion, but that the canonical ERK activator LET-60/Ras may not be required. Through neuron-specific rescue experiments, we found that the ERK pathway functions in head acetylcholine neurons to control Gq-dependent locomotion. Additionally, expression of activated LIN-45/Raf in head acetylcholine neurons is sufficient to cause an exaggerated waveform phenotype and hypersensitivity to the acetylcholinesterase inhibitor aldicarb, similar to an activated Gq mutant. Taken together, our results suggest that the ERK MAPK pathway modulates the output of Gq-Rho signaling to control locomotion behavior in C. elegans.
journal_name
Geneticsjournal_title
Geneticsauthors
Coleman B,Topalidou I,Ailion Mdoi
10.1534/genetics.118.300977subject
Has Abstractpub_date
2018-06-01 00:00:00pages
523-535issue
2eissn
0016-6731issn
1943-2631pii
genetics.118.300977journal_volume
209pub_type
杂志文章相关文献
GENETICS文献大全abstract：:When a polygenic character is exposed to natural selection in which the curve giving fitness as a function of phenotype is a mixture of two Gaussian (normal) curves, the population may respond either by evolving to a specialized phenotype near one of the two optimum phenotypes, or by evolving to a generalized phenotyp...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:Gain or loss of chromosomes resulting in aneuploidy can be important factors in cancer and adaptive evolution. Although chromosome gain is a frequent event in eukaryotes, there is limited information on its genetic control. Here we measured the rates of chromosome gain in wild-type yeast and sister chromatid cohesion ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.159202
更新日期：2014-02-01 00:00:00
abstract：:Polycomb group response elements (PREs) play an essential role in gene regulation by the Polycomb group (PcG) repressor proteins in Drosophila. PREs are required for the recruitment and maintenance of repression by the PcG proteins. PREs are made up of binding sites for multiple DNA-binding proteins, but it is still u...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.153247
更新日期：2013-10-01 00:00:00
abstract：:Hybrid incompatibility can result from gene misregulation produced by divergence in trans-acting regulatory factors and their cis-regulatory targets. However, change in trans-acting factors may be constrained by pleiotropy, which would in turn limit the evolution of incompatibility. We employed a mechanistically expli...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.171397
更新日期：2014-12-01 00:00:00
abstract：:Sex differences occur in most species and affect a variety of biological traits including morphology, behavior, and life history. The nematode Caenorhabditis elegans exists as a population of self-fertile hermaphrodites with occasional males, which differ anatomically and behaviorally from hermaphrodites. Here we show...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.056093
更新日期：2006-06-01 00:00:00
abstract：:X chromosome inactivation (XCI) is an epigenetic process that almost completely inactivates one of two X chromosomes in somatic cells of mammalian females. A few genes are known to escape XCI and the mechanism for this escape remains unclear. Here, using mouse trophoblast stem (TS) cells, we address whether particular...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.162800
更新日期：2014-06-01 00:00:00
abstract：:Mutations in the SPT10 and SPT21 genes were originally isolated as suppressors of Ty and LTR (delta) insertion mutations in Saccharomyces cerevisiae, and the genes were shown to be required for normal transcription at a number of loci in yeast. Now we have cloned, sequenced, mapped and mutagenized SPT10 and SPT21. Sin...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The neutral rate of allelic substitution is analyzed for a class-structured population subject to a stationary stochastic demographic process. The substitution rate is shown to be generally equal to the effective mutation rate, and under overlapping generations it can be expressed as the effective mutation rate in new...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.163345
更新日期：2014-05-01 00:00:00
abstract：:The change in the frequency of a rare mutant allele under constant sex-differentiated viability selection in an infinite, partial full-sib mating population is studied. The diplo-diploid and haplo-diploid polygynous models are considered with a Poisson distribution for the number of offspring produced by every mated f...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.039024
更新日期：2005-09-01 00:00:00
abstract：:A radiation-induced translocation, T(IIR----IIIL), has been shown to be nonreciprocal and to have most of IIR, including its terminus, attached uninverted to the terminus of IIIL.--Progeny with the IIR segment in duplicate, obtained from crosses of T(IIR----IIIL) to strains with a standard genome, were unstable at mit...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:The Drosophila melanogaster Gld gene has multiple and diverse developmental and physiological functions. We report herein that interactions among proximal promoter elements and a cluster of intronically located enhancers and silencers specify the complex regulation of Gld that underlies its diverse functions. Gld expr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:fl(2)d, the Drosophila homolog of Wilms'-tumor-1-associated protein (WTAP), regulates the alternative splicing of Sex-lethal (Sxl), transformer (tra), and Ultrabithorax (Ubx). Although WTAP has been found in functional human spliceosomes, exactly how it contributes to the splicing process remains unknown. Here we atte...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.081679
更新日期：2008-02-01 00:00:00
abstract：:The prune gene of Drosophila melanogaster is predicted to encode a phosphodiesterase. Null alleles of prune are viable but cause an eye-color phenotype. The abnormal wing discs gene encodes a nucleoside diphosphate kinase. Killer of prune is a missense mutation in the abnormal wing discs gene. Although it has no pheno...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.044669
更新日期：2006-01-01 00:00:00
abstract：:A procedure permitting deletion scanning of potential cis-regulatory sequences within a transgene whose genomic position remains fixed was applied to the study of the upstream sequences of the ecdysteroid-inducible Fat-body-protein-1 (Fbp1) gene. Deficiencies were induced in a Fbp1:Adh fusion transgene by means of a s...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:There is increasing evidence that certain Vacuolar protein sorting (Vps) proteins, factors that mediate vesicular protein trafficking, have additional roles in regulating transcription factors at the endosome. We found that yeast mutants lacking the phosphatidylinositol 3-phosphate [PI(3)P] kinase Vps34 or its associa...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.146308
更新日期：2013-03-01 00:00:00
abstract：:The relationships between synonymous and nonsynonymous substitution rates and between synonymous rate and codon usage bias are important to our understanding of the roles of mutation and selection in the evolution of Drosophila genes. Previous studies used approximate estimation methods that ignore codon bias. In this...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Population structure parameters commonly used for diploid species are reexamined for the particular case of tetrasomic inheritance (autotetraploid species). Recurrence equations that describe the evolution of identity probabilities for neutral genes in an "island model" of population structure are derived assuming tet...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:We have examined the in vivo requirement of two recently identified nonessential components of the budding yeast anaphase-promoting complex, Swm1p and Mnd2p, as well as that of the previously identified subunit Apc9p. swm1Delta mutants exhibit synthetic lethality or conditional synthetic lethality with other APC/C sub...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.040105
更新日期：2005-07-01 00:00:00
abstract：:We have characterized tandem duplications in the rII regions of phage T4. The rII deletion r1589 blocks only the function of the rIIA cistron, although it extends into the B cistron. Another rII deletion, r1236, blocks the function of the rIIB cistron and overlaps r1589. When a cross is made between r1589 and r1236, t...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:In eukaryotic cells, checkpoint pathways arrest cell-cycle progression if a particular event has failed to complete appropriately or if an important intracellular structure is defective or damaged. Saccharomyces cerevisiae strains that lack the SFP1 gene fail to arrest at the G2 DNA-damage checkpoint in response to ge...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:We have studied lethal mutations in the single calmodulin gene (Cam) of Drosophila to gain insight into the in vivo functions of this important calcium sensor. As a result of maternal calmodulin (CaM) in the mature egg, lethality is delayed until the postembryonic stages. Prior to death in the first larval instar, Cam...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:Behavior is influenced by an organism's genes and environment, including its interactions with same or opposite sex individuals. Drosophila melanogaster perform innate, yet socially modifiable, courtship behaviors that are sex specific and require rapid integration and response to multiple sensory cues. Furthermore, m...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.122754
更新日期：2011-01-01 00:00:00
abstract：:The Genetics Society of America's (GSA) Edward Novitski Prize is awarded to researchers who have solved challenging problems in genetics through experiments that demonstrate exceptional creativity and ingenuity. Job Dekker of the University of Massachusetts Medical School has been selected for the 2018 award in recogn...
journal_title：Genetics
pub_type： 传,历史文章,杂志文章
doi：10.1534/genetics.118.301641
更新日期：2018-11-01 00:00:00
abstract：:The distribution of the number of HLA haplotypes shared by sibs affected with the same HLA-linked disease can be used to obtain information on the genetics of the disease. Since the inception of the use of sib-pair methods for the analysis of the HLA-associated diseases, the question has been raised of how to include ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Directional export of messenger RNA (mRNA) protein particles (mRNPs) through nuclear pore complexes (NPCs) requires multiple factors. In Saccharomyces cerevisiae, the NPC proteins Nup159 and Nup42 are asymmetrically localized to the cytoplasmic face and have distinct functional domains: a phenylalanine-glycine (FG) re...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.164012
更新日期：2014-08-01 00:00:00
abstract：:Positive selection on a new mutant allele can increase the frequencies of closely linked alleles (through hitchhiking), as well as create linkage disequilibrium between them. Because this disequilibrium is induced by the selected allele, one may be able to identify loci under selection by measuring the influence of a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:The island model with stochastically variable migration rate and immigrant gene frequency is investigated. It is supposed that the migration rate and the immigrant gene frequency are independent of each other in each generation, and each of them is independently and identically distributed in every generation. The tre...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The morphogenetic pathway of bacteriophage phi X 174 was investigated in rep mutant hosts that specifically block stage III single-stranded DNA synthesis. The defects conferred by the mutant rep protein most likely affect the formation or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:The initiation of growth cessation and dormancy represents a critical ecological and evolutionary trade-off between survival and growth in most forest trees. The most important environmental cue regulating the initiation of dormancy is a shortening of the photoperiod and phytochrome genes have been implicated in short...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.047522
更新日期：2006-03-01 00:00:00
abstract：:A large number of arginine-requiring mutants of Neurospora was isolated, using a strain already partially impaired in an enzyme of the pathway. Among the mutants, all previously described loci, except one, were represented, and several new loci were defined and mapped. Four groups of mutants were of particular interes...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
