Abstract:
:The functional consequences of long-range nuclear reorganization were studied in a cell-by-cell analysis of gene expression and long-range chromosomal interactions in the Drosophila eye and eye imaginal disk. Position-effect variegation was used to stochastically perturb gene expression and probe nuclear reorganization. Variegating genes on rearrangements of Chromosomes X, 2, and 3 were probed for long-range interactions with heterochromatin. Studies were conducted only in tissues known to express the variegating genes. Nuclear structure was revealed by fluorescence in situ hybridization with probes to the variegating gene and heterochromatin. Gene expression was determined alternately by immunofluorescence against specific proteins and by eye pigment autofluorescence. This allowed cell-by-cell comparisons of nuclear architecture between cells in which the variegating gene was either expressed or silenced. Very strong correlations between heterochromatic association and silencing were found. Expressing cells showed a broad distribution of distances between variegating genes and their own centromeric heterochromatin, while silenced cells showed a very tight distribution centered around very short distances, consistent with interaction between the silenced genes and heterochromatin. Spatial and temporal analysis of interactions with heterochromatin indicated that variegating genes primarily associate with heterochromatin in cells that have exited the cell cycle. Differentiation was not a requirement for association, and no differences in association were observed between cell types. Thus, long-range interactions between distal chromosome regions and their own heterochromatin have functional consequences for the organism.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Harmon B,Sedat Jdoi
10.1371/journal.pbio.0030067keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
e67issue
3eissn
1544-9173issn
1545-7885pii
04-PLBI-RA-0361R2journal_volume
3pub_type
杂志文章相关文献
PLOS BIOLOGY文献大全abstract::Single-cell imaging, combined with recent advances in image analysis and microfluidic technologies, have enabled fundamental discoveries of cellular responses to chemical perturbations that are often obscured by traditional liquid-culture experiments. Temperature is an environmental variable well known to impact growt...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000786
更新日期:2020-11-06 00:00:00
abstract::In nature, Escherichia coli are exposed to harsh and non-ideal growth environments-nutrients may be limiting, and cells are often challenged by oxidative stress. For E. coli cells confronting these realities, there appears to be a link between oxidative stress, methionine availability, and the enzyme that catalyzes th...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020336
更新日期:2004-11-01 00:00:00
abstract::DEAD-box proteins are ubiquitous regulators of RNA biology. While commonly dubbed "helicases," their activities also include duplex annealing, adenosine triphosphate (ATP)-dependent RNA binding, and RNA-protein complex remodeling. Rok1, an essential DEAD-box protein, and its cofactor Rrp5 are required for ribosome ass...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002480
更新日期:2016-06-09 00:00:00
abstract::The Open Science Prize was established with the following objectives: first, to encourage the crowdsourcing of open data to make breakthroughs that are of biomedical significance; second, to illustrate that funders can indeed work together when scientific interests are aligned; and finally, to encourage international ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2002617
更新日期:2017-08-01 00:00:00
abstract::Understanding the molecular events that underlie the evolution of morphological diversity is a major challenge in biology. Here, to identify genes whose expression correlates with species-specific morphologies, we compared transcriptomes of two closely related Hydra species. We find that species-specific differences i...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0060278
更新日期:2008-11-18 00:00:00
abstract::The F-type ATP synthase complex is a rotary nano-motor driven by proton motive force to synthesize ATP. Its F(1) sector catalyzes ATP synthesis, whereas the F(o) sector conducts the protons and provides a stator for the rotary action of the complex. Components of both F(1) and F(o) sectors are highly conserved across ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000418
更新日期:2010-07-13 00:00:00
abstract::Horizontal DNA transfer (HDT) is a pervasive mechanism of diversification in many microbial species, but its primary evolutionary role remains controversial. Much recent research has emphasised the adaptive benefit of acquiring novel DNA, but here we argue instead that intragenomic conflict provides a coherent framewo...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002394
更新日期:2016-03-02 00:00:00
abstract::The deleterious effects of prolonged sleep deprivation on behavior and cognition are a concern in modern society. Persons at risk for impaired performance and health-related issues resulting from prolonged sleep loss would benefit from agents capable of reducing these detrimental effects at the time they are sleep dep...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030299
更新日期:2005-09-01 00:00:00
abstract::The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfol...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000102
更新日期:2009-05-01 00:00:00
abstract::The segmentation gene network of Drosophila consists of maternal and zygotic factors that generate, by transcriptional (cross-) regulation, expression patterns of increasing complexity along the anterior-posterior axis of the embryo. Using known binding site information for maternal and zygotic gap transcription facto...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020271
更新日期:2004-09-01 00:00:00
abstract::Cell motility is essential for protozoan and metazoan organisms and typically relies on the dynamic turnover of actin filaments. In metazoans, monomeric actin polymerises into usually long and stable filaments, while some protozoans form only short and highly dynamic actin filaments. These different dynamics are partl...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2005345
更新日期:2018-07-16 00:00:00
abstract::Within the human host, the malaria parasite Plasmodium falciparum is exposed to multiple selection pressures. The host environment changes dramatically in severe malaria, but the extent to which the parasite responds to-or is selected by-this environment remains unclear. From previous studies, the parasites that cause...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2004328
更新日期:2018-03-12 00:00:00
abstract::During in vitro fertilisation (IVF), pharmacological activation of the murine X chromosome-encoded receptor proteins Toll-like receptor (TLR) 7 and TLR8 reportedly results in male-biased litters by selectively disrupting the motility of X-bearing sperm cells. Thus-in the context of agonist treatment during IVF-these r...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000663
更新日期:2020-03-23 00:00:00
abstract::From a research perspective, the interest in biobanking continues to intensify. Governments and industry have invested heavily in biobanks, as exemplified by initiatives like the United Kingdom Biobank and United States' Precision Medicine Initiative. But despite this enthusiasm, many profound legal and ethical challe...
journal_title:PLoS biology
pub_type: 杂志文章,评审
doi:10.1371/journal.pbio.2002654
更新日期:2017-07-25 00:00:00
abstract::Blood vascular networks in vertebrates are essential to tissue survival. Establishment of a fully functional vasculature is complex and requires a number of steps including vasculogenesis and angiogenesis that are followed by differentiation into specialized vascular tissues (i.e., arteries, veins, and lymphatics) and...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002148
更新日期:2015-05-15 00:00:00
abstract::5-hydroxymethylcytosine (5hmC) is the most prevalent intermediate on the oxidative DNA demethylation pathway and is implicated in regulation of embryogenesis, neurological processes, and cancerogenesis. Profiling of this relatively scarce genomic modification in clinical samples requires cost-effective high-resolution...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000684
更新日期:2020-04-10 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pbio.3000311.]. ...
journal_title:PLoS biology
pub_type: 已发布勘误
doi:10.1371/journal.pbio.3000514
更新日期:2019-10-04 00:00:00
abstract::In their competition for hosts, parasites with antigens that are novel to the host immune system will be at a competitive advantage. The resulting frequency-dependent selection can structure parasite populations into strains of limited genetic overlap. For the causative agent of malaria, Plasmodium falciparum, the hig...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000336
更新日期:2019-06-24 00:00:00
abstract::Mast cells are implicated in the pathogenesis of inflammatory and autoimmune diseases. However, this notion based on studies in mast cell-deficient mice is controversial. We therefore established an in vivo model for hyperactive mast cells by specifically ablating the NF-κB negative feedback regulator A20. While A20 d...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001762
更新日期:2014-01-01 00:00:00
abstract::The array of vibrissae on a rat's face is the first stage in a high-resolution tactile sensing system. Progressing from rostral to caudal in any vibrissae row results in an increase in whisker length and thickness. This may, in turn, provide a systematic map of separate tactile channels governed by the mechanical prop...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000699
更新日期:2020-10-22 00:00:00
abstract::One of the major challenges of functional genomics is to unravel the connection between genotype and phenotype. So far no global analysis has attempted to explore those connections in the light of the large phenotypic variability seen in nature. Here, we use an unsupervised, systematic approach for associating genes a...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030134
更新日期:2005-05-01 00:00:00
abstract::A substantial proportion of protein interactions relies on small domains binding to short peptides in the partner proteins. Many of these interactions are relatively low affinity and transient, and they impact on signal transduction. However, neither the number of potential interactions mediated by each domain nor the...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020014
更新日期:2004-01-01 00:00:00
abstract::Better understanding of feeding behaviour will be vital in reducing obesity and metabolic syndrome, but we lack a standard model that captures the complexity of feeding behaviour. We construct an accurate stochastic model of rodent feeding at the bout level in order to perform quantitative behavioural analysis. Analys...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000482
更新日期:2019-12-05 00:00:00
abstract::The RecA protein forms nucleoprotein filaments on DNA, and individual monomers within the filaments hydrolyze ATP. Assembly and disassembly of filaments are both unidirectional, occurring on opposite filament ends, with disassembly requiring ATP hydrolysis. When filaments form on duplex DNA, RecA protein exhibits a fu...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030052
更新日期:2005-02-01 00:00:00
abstract::Over the last decades, researchers have characterized a set of "clock genes" that drive daily rhythms in physiology and behavior. This arduous work has yielded results with far-reaching consequences in metabolic, psychiatric, and neoplastic disorders. Recent attempts to expand our understanding of circadian regulation...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001840
更新日期:2014-04-15 00:00:00
abstract::Adaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP complexes have been identified, which are present in most eukaryotes. We report the existence of a fifth AP complex, AP-5. Tagged AP-5 localises to a late endosomal compartment i...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001170
更新日期:2011-10-01 00:00:00
abstract::Low reproducibility rates within life science research undermine cumulative knowledge production and contribute to both delays and costs of therapeutic drug development. An analysis of past studies indicates that the cumulative (total) prevalence of irreproducible preclinical research exceeds 50%, resulting in approxi...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002165
更新日期:2015-06-09 00:00:00
abstract::Pheromones form an essential chemical language of intraspecific communication in many animals. How olfactory systems recognize pheromonal signals with both sensitivity and specificity is not well understood. An important in vivo paradigm for this process is the detection mechanism of the sex pheromone (Z)-11-octadecen...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001546
更新日期:2013-01-01 00:00:00
abstract::Pruning that selectively eliminates unnecessary axons/dendrites is crucial for sculpting the nervous system during development. During Drosophila metamorphosis, dendrite arborization neurons, ddaCs, selectively prune their larval dendrites in response to the steroid hormone ecdysone, whereas mushroom body γ neurons sp...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001657
更新日期:2013-09-01 00:00:00
abstract::Flexible behavior is critical for everyday decision-making and has been implicated in restricted, repetitive behaviors (RRB) in autism spectrum disorder (ASD). However, how flexible behavior changes developmentally in ASD remains largely unknown. Here, we used a developmental approach and examined flexible behavior on...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000908
更新日期:2020-10-27 00:00:00