The Economics of Reproducibility in Preclinical Research.

abstract：:We used a class of designed peptide detergents to stabilize photosystem I (PS-I) upon extended drying under N2 on a gold-coated-Ni-NTA glass surface. PS-I is a chlorophyll-containing membrane protein complex that is the primary reducer of ferredoxin and the electron acceptor of plastocyanin. We isolated the complex fr...

journal_title：PLoS biology

authors： Kiley P,Zhao X,Vaughn M,Baldo MA,Bruce BD,Zhang S

更新日期：2005-07-01 00:00:00

abstract：:Atypical chemokine receptor 2 (ACKR2) is a chemokine-scavenging receptor. ACKR2-/-embryos display a reduction in size of a novel, to our knowledge, embryonic skin macrophage population referred to as 'intermediate' cells. CC chemokine receptor 2 (CCR2)-/-embryos display an identical phenotype, indicating that these ce...

journal_title：PLoS biology

authors： Lee KM,Wilson GJ,Pingen M,Fukuoka A,Hansell CAH,Bartolini R,Medina-Ruiz L,Graham GJ

更新日期：2019-05-29 00:00:00

abstract：:Recent brain imaging studies using functional magnetic resonance imaging (fMRI) have implicated a frontal-parietal network in the top-down control of attention. However, little is known about the timing and sequence of activations within this network. To investigate these timing questions, we used event-related electr...

journal_title：PLoS biology

authors： Grent-'t-Jong T,Woldorff MG

更新日期：2007-01-01 00:00:00

abstract：:The sperm's crucial function is to locate and fuse with a mature oocyte. Under laboratory conditions, Caenorhabditis elegans sperm are very efficient at navigating the hermaphrodite reproductive tract and locating oocytes. Here, we identify chemosensory and oxygen-sensing circuits that affect the sperm's navigational ...

journal_title：PLoS biology

authors： Hoang HD,Miller MA

更新日期：2017-06-29 00:00:00

abstract：:Smallpox is unique among infectious diseases in the degree to which it devastated human populations, its long history of control interventions, and the fact that it has been successfully eradicated. Mortality from smallpox in London, England was carefully documented, weekly, for nearly 300 years, providing a rare and ...

journal_title：PLoS biology

authors： Krylova O,Earn DJD

更新日期：2020-12-21 00:00:00

abstract：:Misalignment of the daily sleep-wake and fasting-feeding cycles with the endogenous circadian timing system is an inevitable consequence of night shift work and is associated with adverse metabolic health effects. However, a detailed characterisation of the effects of night shifts on 24-h rhythms in the metabolome is ...

journal_title：PLoS biology

authors： Kervezee L,Cermakian N,Boivin DB

更新日期：2019-06-18 00:00:00

abstract：:The mechanisms by which genes control organ shape are poorly understood. In principle, genes may control shape by modifying local rates and/or orientations of deformation. Distinguishing between these possibilities has been difficult because of interactions between patterns, orientations, and mechanical constraints du...

journal_title：PLoS biology

authors： Green AA,Kennaway JR,Hanna AI,Bangham JA,Coen E

更新日期：2010-11-09 00:00:00

abstract：:The organization and the mechanisms of condensation of mitotic chromosomes remain unsolved despite many decades of efforts. The lack of resolution, tight compaction, and the absence of function-specific chromatin labels have been the key technical obstacles. The correlation between DNA sequence composition and its con...

journal_title：PLoS biology

authors： Strukov YG,Sural TH,Kuroda MI,Sedat JW

更新日期：2011-01-11 00:00:00

abstract：:Genome-wide studies of circadian transcription or mRNA translation have been hindered by the presence of heterogeneous cell populations in complex tissues such as the nervous system. We describe here the use of a Drosophila cell-specific translational profiling approach to document the rhythmic "translatome" of neural...

journal_title：PLoS biology

authors： Huang Y,Ainsley JA,Reijmers LG,Jackson FR

更新日期：2013-11-01 00:00:00

abstract：:Eukaryotic cells contain several unconventional poly(A) polymerases in addition to the canonical enzymes responsible for the synthesis of poly(A) tails of nuclear messenger RNA precursors. The yeast protein Trf4p has been implicated in a quality control pathway that leads to the polyadenylation and subsequent exosome-...

journal_title：PLoS biology

authors： Vanácová S,Wolf J,Martin G,Blank D,Dettwiler S,Friedlein A,Langen H,Keith G,Keller W

更新日期：2005-06-01 00:00:00

abstract：:The gene encoding the transcriptional coactivator peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) was targeted in mice. PGC-1alpha null (PGC-1alpha(-/-)) mice were viable. However, extensive phenotyping revealed multi-system abnormalities indicative of an abnormal energy metabolic phen...

journal_title：PLoS biology

authors： Leone TC,Lehman JJ,Finck BN,Schaeffer PJ,Wende AR,Boudina S,Courtois M,Wozniak DF,Sambandam N,Bernal-Mizrachi C,Chen Z,Holloszy JO,Medeiros DM,Schmidt RE,Saffitz JE,Abel ED,Semenkovich CF,Kelly DP

更新日期：2005-04-01 00:00:00

abstract：:Polarized growth is maintained by both polarized exocytosis, which transports membrane components to specific locations on the cell cortex, and endocytosis, which retrieves these components before they can diffuse away. Despite functional links between these two transport pathways, they are generally considered to be ...

journal_title：PLoS biology

authors： Johansen J,Alfaro G,Beh CT

更新日期：2016-08-15 00:00:00

abstract：:Motion is an essential component of everyday tactile experience: most manual interactions involve relative movement between the skin and objects. Much of the research on the neural basis of tactile motion perception has focused on how direction is encoded, but less is known about how speed is. Perceived speed has been...

journal_title：PLoS biology

authors： Delhaye BP,O'Donnell MK,Lieber JD,McLellan KR,Bensmaia SJ

更新日期：2019-08-27 00:00:00

abstract：:Although psychological and computational models of time estimation have postulated the existence of neural representations tuned for specific durations, empirical evidence of this notion has been lacking. Here, using a functional magnetic resonance imaging (fMRI) adaptation paradigm, we show that the inferior parietal...

journal_title：PLoS biology

authors： Hayashi MJ,Ditye T,Harada T,Hashiguchi M,Sadato N,Carlson S,Walsh V,Kanai R

更新日期：2015-09-17 00:00:00

abstract：:Mitochondrial shape is determined by fission and fusion reactions catalyzed by large GTPases of the dynamin family, mutation of which can cause neurological dysfunction. While fission-inducing protein phosphatases have been identified, the identity of opposing kinase signaling complexes has remained elusive. We report...

journal_title：PLoS biology

authors： Merrill RA,Dagda RK,Dickey AS,Cribbs JT,Green SH,Usachev YM,Strack S

更新日期：2011-04-01 00:00:00

abstract：:How should funding agencies enable researchers to explore high-risk but potentially high-reward science? One model that appears to work is the NSF-funded synthesis center, an incubator for community-led, innovative science. ...

journal_title：PLoS biology

authors： Rodrigo A,Alberts S,Cranston K,Kingsolver J,Lapp H,McClain C,Smith R,Vision T,Weintraub J,Wiegmann B

更新日期：2013-01-01 00:00:00

abstract：:Mitochondrial complex I, the largest and most complicated proton pump of the respiratory chain, links the electron transfer from NADH to ubiquinone to the pumping of four protons from the matrix into the intermembrane space. In humans, defects in complex I are involved in a wide range of degenerative disorders. Recent...

journal_title：PLoS biology

authors： Dröse S,Krack S,Sokolova L,Zwicker K,Barth HD,Morgner N,Heide H,Steger M,Nübel E,Zickermann V,Kerscher S,Brutschy B,Radermacher M,Brandt U

更新日期：2011-08-01 00:00:00

abstract：:The development of axonal arbors is a critical step in the establishment of precise neural circuits, but relatively little is known about the mechanisms of axonal elaboration in the neocortex. We used in vivo two-photon time-lapse microscopy to image axons in the neocortex of green fluorescent protein-transgenic mice ...

journal_title：PLoS biology

authors： Portera-Cailliau C,Weimer RM,De Paola V,Caroni P,Svoboda K

更新日期：2005-08-01 00:00:00

abstract：:Self-incompatibility (SI) is the flowering plant reproductive system in which self pollen tube growth is inhibited, thereby preventing self-fertilization. SI has evolved independently in several different flowering plant lineages. In all Brassicaceae species in which the molecular basis of SI has been investigated in ...

journal_title：PLoS biology

authors： Chantha SC,Herman AC,Platts AE,Vekemans X,Schoen DJ

更新日期：2013-01-01 00:00:00

abstract：:In this Open Highlight, Senior Editor Lauren Richardson features exciting new Open Access research into how species evolve their characteristic traits. ...

journal_title：PLoS biology

authors： Richardson LA

更新日期：2018-10-04 00:00:00

abstract：:Rapidly grouping local elements into an organized object (i.e., perceptual integration) is a fundamental yet challenging task, especially in noisy contexts. Previous studies demonstrate that ventral visual pathway, which is widely known to mediate object recognition, engages in the process by conveying object-level in...

journal_title：PLoS biology

authors： Liu L,Wang F,Zhou K,Ding N,Luo H

更新日期：2017-11-30 00:00:00

abstract：:The oversupply of postdoctoral scholars relative to available faculty positions has led to calls for better assessment of career outcomes. Here, we report the results of a study of postdoctoral outcomes at the University of California, San Francisco, and suggest that institutions have an obligation to determine where ...

journal_title：PLoS biology

authors： Silva EA,Des Jarlais C,Lindstaedt B,Rotman E,Watkins ES

更新日期：2016-05-06 00:00:00

abstract：:Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division. We show here that, after nuclear transfer to amphibian oocytes, mitotic...

journal_title：PLoS biology

authors： Halley-Stott RP,Jullien J,Pasque V,Gurdon J

更新日期：2014-07-29 00:00:00

abstract：:Microbial endosymbiosis is widespread in animals, with major ecological and evolutionary implications. Successful symbiosis relies on efficient vertical transmission through host generations. However, when symbionts negatively affect host fitness, hosts are expected to evolve suppression of symbiont effects or transmi...

journal_title：PLoS biology

authors： Becking T,Chebbi MA,Giraud I,Moumen B,Laverré T,Caubet Y,Peccoud J,Gilbert C,Cordaux R

更新日期：2019-10-10 00:00:00

abstract：:In this Formal Comment, the authors of the recent publication "Large-scale investigation of the reasons why potentially important genes are ignored" maintain that it can be read as an opportunity to explore the unknown. ...

journal_title：PLoS biology

authors： Stoeger T,Gerlach M,Morimoto RI,Amaral LAN

更新日期：2018-12-11 00:00:00

abstract：:Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Droso...

journal_title：PLoS biology

authors： Dobbelaere J,Josué F,Suijkerbuijk S,Baum B,Tapon N,Raff J

更新日期：2008-09-16 00:00:00

abstract：:FtsZ is a tubulin-like GTPase that is the major cytoskeletal protein in bacterial cell division. It polymerizes into a ring, called the Z ring, at the division site and acts as a scaffold to recruit other division proteins to this site as well as providing a contractile force for cytokinesis. To understand how FtsZ pe...

journal_title：PLoS biology

authors： Strauss MP,Liew AT,Turnbull L,Whitchurch CB,Monahan LG,Harry EJ

更新日期：2012-01-01 00:00:00

abstract：:Domestic dogs exhibit tremendous phenotypic diversity, including a greater variation in body size than any other terrestrial mammal. Here, we generate a high density map of canine genetic variation by genotyping 915 dogs from 80 domestic dog breeds, 83 wild canids, and 10 outbred African shelter dogs across 60,968 sin...

journal_title：PLoS biology

authors： Boyko AR,Quignon P,Li L,Schoenebeck JJ,Degenhardt JD,Lohmueller KE,Zhao K,Brisbin A,Parker HG,vonHoldt BM,Cargill M,Auton A,Reynolds A,Elkahloun AG,Castelhano M,Mosher DS,Sutter NB,Johnson GS,Novembre J,Hubisz MJ,

更新日期：2010-08-10 00:00:00

abstract：:Endosomal trafficking of receptors and associated proteins plays a critical role in signal processing. Until recently, it was thought that trafficking was shut down during cell division. Thus, remarkably, the regulation of trafficking during division remains poorly characterized. Here we delineate the role of mitotic ...

journal_title：PLoS biology

authors： Cota CD,Dreier MS,Colgan W,Cha A,Sia T,Davidson B

更新日期：2021-01-04 00:00:00

abstract：:While the timing of neuronal activity in the olfactory bulb (OB) relative to sniffing has been the object of many studies, the behavioral relevance of timing information generated by patterned activation within the bulbar response has not been explored. Here we show, using sniff-triggered, dynamic, 2-D, optogenetic st...

journal_title：PLoS biology

authors： Rebello MR,McTavish TS,Willhite DC,Short SM,Shepherd GM,Verhagen JV

更新日期：2014-12-16 00:00:00