Abstract:
:Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division. We show here that, after nuclear transfer to amphibian oocytes, mitotic chromatin is reprogrammed up to 100 times faster than interphase nuclei. We find that, as cells traverse mitosis, their genes pass through a temporary phase of unusually high responsiveness to oocyte reprogramming factors (mitotic advantage). Mitotic advantage is not explained by nuclear penetration, DNA modifications, histone acetylation, phosphorylation, methylation, nor by salt soluble chromosomal proteins. Our results suggest that histone H2A deubiquitination may account, at least in part, for the acquisition of mitotic advantage. They support the general principle that a temporary access of cytoplasmic factors to genes during mitosis may facilitate somatic cell nuclear reprogramming and the acquisition of new cell fates in normal development.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Halley-Stott RP,Jullien J,Pasque V,Gurdon Jdoi
10.1371/journal.pbio.1001914subject
Has Abstractpub_date
2014-07-29 00:00:00pages
e1001914issue
7eissn
1544-9173issn
1545-7885pii
PBIOLOGY-D-14-00928journal_volume
12pub_type
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