当前位置： SCI文献检索 > PLOS BIOLOGY期刊下所有文献 > Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time.

Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time.

Abstract：

:We used a class of designed peptide detergents to stabilize photosystem I (PS-I) upon extended drying under N2 on a gold-coated-Ni-NTA glass surface. PS-I is a chlorophyll-containing membrane protein complex that is the primary reducer of ferredoxin and the electron acceptor of plastocyanin. We isolated the complex from the thylakoids of spinach chloroplasts using a chemical detergent. The chlorophyll molecules associated with the PS-I complex provide an intrinsic steady-state emission spectrum between 650 and 800 nm at -196.15 degrees C that reflects the organization of the pigment-protein interactions. In the absence of detergents, a large blue shift of the fluorescence maxima from approximately 735 nm to approximately 685 nm indicates a disruption in light-harvesting subunit organization, thus revealing chlorophyll-protein interactions. The commonly used membrane protein-stabilizing detergents, N-dodecyl-beta-D-maltoside and N-octyl-beta-D-glucoside, only partially stabilized the approximately 735-nm complex with approximately 685-nm spectroscopic shift. However, prior to drying, addition of the peptide detergent acetyl-AAAAAAK at increasing concentration significantly stabilized the PS-I complex. Moreover, in the presence of acetyl-AAAAAAK, the PS-I complex is stable in a dried form at room temperature for at least 3 wk. Another peptide detergent, acetyl-VVVVVVD, also stabilized the complex but to a lesser extent. These observations suggest that the peptide detergents may effectively stabilize membrane proteins in the solid-state. These designed peptide detergents may facilitate the study of diverse types of membrane proteins.

journal_name

PLoS Biol

journal_title

PLoS biology

authors

Kiley P,Zhao X,Vaughn M,Baldo MA,Bruce BD,Zhang S

doi

10.1371/journal.pbio.0030230

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

e230

issue

7

eissn

1544-9173

issn

1545-7885

pii

04-PLBI-RA-0731R2

journal_volume

3

pub_type

杂志文章

相关文献

PLOS BIOLOGY文献大全
  • mRNA association by aminoacyl tRNA synthetase occurs at a putative anticodon mimic and autoregulates translation in response to tRNA levels.

    abstract：:Aminoacyl-tRNA synthetases (aaRSs) are well studied for their role in binding and charging tRNAs with cognate amino acids. Recent RNA interactome studies had suggested that these enzymes can also bind polyadenylated RNAs. Here, we explored the mRNA repertoire bound by several yeast aaRSs. RNA immunoprecipitation (RIP)...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.3000274

    authors： Levi O,Arava Y

    更新日期：2019-05-17 00:00:00

  • Structural and mechanistic basis of zinc regulation across the E. coli Zur regulon.

    abstract：:Commensal microbes, whether they are beneficial or pathogenic, are sensitive to host processes that starve or swamp the prokaryote with large fluctuations in local zinc concentration. To understand how microorganisms coordinate a dynamic response to changes in zinc availability at the molecular level, we evaluated the...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1001987

    authors： Gilston BA,Wang S,Marcus MD,Canalizo-Hernández MA,Swindell EP,Xue Y,Mondragón A,O'Halloran TV

    更新日期：2014-11-04 00:00:00

  • Self-organizing spots get under your skin.

    abstract：:Sixty-five years after Turing first revealed the potential of systems with local activation and long-range inhibition to generate pattern, we have only recently begun to identify the biological elements that operate at many scales to generate periodic patterns in nature. In this Primer, we first review the theoretical...

    journal_title：PLoS biology

    pub_type： 杂志文章,评审

    doi：10.1371/journal.pbio.2004412

    authors： Dalle Nogare D,Chitnis AB

    更新日期：2017-12-20 00:00:00

  • Cadherin-2 controls directional chain migration of cerebellar granule neurons.

    abstract：:Long distance migration of differentiating granule cells from the cerebellar upper rhombic lip has been reported in many vertebrates. However, the knowledge about the subcellular dynamics and molecular mechanisms regulating directional neuronal migration in vivo is just beginning to emerge. Here we show by time-lapse ...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1000240

    authors： Rieger S,Senghaas N,Walch A,Köster RW

    更新日期：2009-11-01 00:00:00

  • A helical structural nucleus is the primary elongating unit of insulin amyloid fibrils.

    abstract：:Although amyloid fibrillation is generally believed to be a nucleation-dependent process, the nuclei are largely structurally uncharacterized. This is in part due to the inherent experimental challenge associated with structural descriptions of individual components in a dynamic multi-component equilibrium. There are ...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.0050134

    authors： Vestergaard B,Groenning M,Roessle M,Kastrup JS,van de Weert M,Flink JM,Frokjaer S,Gajhede M,Svergun DI

    更新日期：2007-05-01 00:00:00

  • Inferring the dynamics of diversification: a coalescent approach.

    abstract：:Recent analyses of the fossil record and molecular phylogenies suggest that there are fundamental limits to biodiversity, possibly arising from constraints in the availability of space, resources, or ecological niches. Under this hypothesis, speciation rates decay over time and biodiversity eventually saturates, with ...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1000493

    authors： Morlon H,Potts MD,Plotkin JB

    更新日期：2010-09-28 00:00:00

  • Smek promotes corticogenesis through regulating Mbd3's stability and Mbd3/NuRD complex recruitment to genes associated with neurogenesis.

    abstract：:The fate of neural progenitor cells (NPCs) during corticogenesis is determined by a complex interplay of genetic or epigenetic components, but the underlying mechanism is incompletely understood. Here, we demonstrate that Suppressor of Mek null (Smek) interact with methyl-CpG-binding domain 3 (Mbd3) and the complex pl...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.2001220

    authors： Moon BS,Yun HM,Chang WH,Steele BH,Cai M,Choi SH,Lu W

    更新日期：2017-05-03 00:00:00

  • In TFIIH, XPD helicase is exclusively devoted to DNA repair.

    abstract：:The eukaryotic XPD helicase is an essential subunit of TFIIH involved in both transcription and nucleotide excision repair (NER). Mutations in human XPD are associated with several inherited diseases such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. We performed a comparative analysis of XPD f...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1001954

    authors： Kuper J,Braun C,Elias A,Michels G,Sauer F,Schmitt DR,Poterszman A,Egly JM,Kisker C

    更新日期：2014-09-30 00:00:00

  • Why is congenital Zika syndrome asymmetrically distributed among human populations?

    abstract：:Zika virus (ZIKV) is a health burden due to the severe neurological abnormalities that arise after congenital infection. Although multiple experimental studies have linked ZIKV with neural birth defects, the scientific community has not been able to fully explain why Congenital Zika Syndrome (CZS) was only apparent af...

    journal_title：PLoS biology

    pub_type： 杂志文章,评审

    doi：10.1371/journal.pbio.2006592

    authors： Barbeito-Andrés J,Schuler-Faccini L,Garcez PP

    更新日期：2018-08-24 00:00:00

  • The COVID-19 response illustrates that traditional academic reward structures and metrics do not reflect crucial contributions to modern science.

    abstract：:The COVID-19 pandemic has motivated many open and collaborative analytical research projects with real-world impact. However, despite their value, such activities are generally overlooked by traditional academic metrics. Science is ultimately improved by analytical work, whether ensuring reproducible and well-document...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.3000913

    authors： Kucharski AJ,Funk S,Eggo RM

    更新日期：2020-10-16 00:00:00

  • Precise positioning of myosin VI on endocytic vesicles in vivo.

    abstract：:Myosin VI has been studied in both a monomeric and a dimeric form in vitro. Because the functional characteristics of the motor are dramatically different for these two forms, it is important to understand whether myosin VI heavy chains are brought together on endocytic vesicles. We have used fluorescence anisotropy m...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.0050210

    authors： Altman D,Goswami D,Hasson T,Spudich JA,Mayor S

    更新日期：2007-08-01 00:00:00

  • Correction: Tracking Resilience to Infections by Mapping Disease Space.

    abstract：:[This corrects the article DOI: 10.1371/journal.pbio.1002436.]. ...

    journal_title：PLoS biology

    pub_type： 已发布勘误

    doi：10.1371/journal.pbio.1002494

    authors： PLOS Biology Staff.

    更新日期：2016-06-08 00:00:00

  • Enhancement of SMN2 exon 7 inclusion by antisense oligonucleotides targeting the exon.

    abstract：:Several strategies have been pursued to increase the extent of exon 7 inclusion during splicing of SMN2 (survival of motor neuron 2) transcripts, for eventual therapeutic use in spinal muscular atrophy (SMA), a genetic neuromuscular disease. Antisense oligonucleotides (ASOs) that target an exon or its flanking splice ...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.0050073

    authors： Hua Y,Vickers TA,Baker BF,Bennett CF,Krainer AR

    更新日期：2007-04-01 00:00:00

  • Brain Connectivity Predicts Placebo Response across Chronic Pain Clinical Trials.

    abstract：:Placebo response in the clinical trial setting is poorly understood and alleged to be driven by statistical confounds, and its biological underpinnings are questioned. Here we identified and validated that clinical placebo response is predictable from resting-state functional magnetic-resonance-imaging (fMRI) brain co...

    journal_title：PLoS biology

    pub_type： 杂志文章,随机对照试验

    doi：10.1371/journal.pbio.1002570

    authors： Tétreault P,Mansour A,Vachon-Presseau E,Schnitzer TJ,Apkarian AV,Baliki MN

    更新日期：2016-10-27 00:00:00

  • Determination of stromal signatures in breast carcinoma.

    abstract：:Many soft tissue tumors recapitulate features of normal connective tissue. We hypothesize that different types of fibroblastic tumors are representative of different populations of fibroblastic cells or different activation states of these cells. We examined two tumors with fibroblastic features, solitary fibrous tumo...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.0030187

    authors： West RB,Nuyten DS,Subramanian S,Nielsen TO,Corless CL,Rubin BP,Montgomery K,Zhu S,Patel R,Hernandez-Boussard T,Goldblum JR,Brown PO,van de Vijver M,van de Rijn M

    更新日期：2005-06-01 00:00:00

  • Correction: Regulating toxic chemicals for public and environmental health.

    abstract：:[This corrects the article DOI: 10.1371/journal.pbio.2004814.]. ...

    journal_title：PLoS biology

    pub_type： 杂志文章,已发布勘误

    doi：10.1371/journal.pbio.1002619

    authors： PLOS Biology Staff.

    更新日期：2018-01-25 00:00:00

  • Haves and have nots must find a better way: The case for open scientific hardware.

    abstract：:Many efforts are making science more open and accessible; they are mostly concentrated on issues that appear before and after experiments are performed: open access journals, open databases, and many other tools to increase reproducibility of science and access to information. However, these initiatives do not promote...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.3000014

    authors： Maia Chagas A

    更新日期：2018-09-27 00:00:00

  • Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.

    abstract：:In adults, the growth of blood vessels, a process known as angiogenesis, is essential for organ growth and repair. In many disorders including cancer, angiogenesis becomes excessive. The cellular origin of new vascular endothelial cells (ECs) during blood vessel growth in angiogenic situations has remained unknown. He...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1001407

    authors： Fang S,Wei J,Pentinmikko N,Leinonen H,Salven P

    更新日期：2012-01-01 00:00:00

  • Hypoxia-inducible factor directs POMC gene to mediate hypothalamic glucose sensing and energy balance regulation.

    abstract：:Hypoxia-inducible factor (HIF) is a nuclear transcription factor that responds to environmental and pathological hypoxia to induce metabolic adaptation, vascular growth, and cell survival. Here we found that HIF subunits and HIF2α in particular were normally expressed in the mediobasal hypothalamus of mice. Hypothalam...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1001112

    authors： Zhang H,Zhang G,Gonzalez FJ,Park SM,Cai D

    更新日期：2011-07-01 00:00:00

  • Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2.

    abstract：:The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the topology of mPRβ is similar to adiponectin recepto...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.3000901

    authors： Nader N,Dib M,Hodeify R,Courjaret R,Elmi A,Hammad AS,Dey R,Huang XY,Machaca K

    更新日期：2020-11-02 00:00:00

  • Moving developmental social neuroscience toward a second-person approach.

    abstract：:Infants' cognitive development and learning rely profoundly on their interactions with other people. In the first year, infants become increasingly sensitive to others' gaze and use it to focus their own attention on relevant visual input. However, infants are not passive observers in early social interactions, and th...

    journal_title：PLoS biology

    pub_type： 评论,杂志文章

    doi：10.1371/journal.pbio.3000055

    authors： Hoehl S,Markova G

    更新日期：2018-12-13 00:00:00

  • Single-cell RNA sequencing reveals intrinsic and extrinsic regulatory heterogeneity in yeast responding to stress.

    abstract：:From bacteria to humans, individual cells within isogenic populations can show significant variation in stress tolerance, but the nature of this heterogeneity is not clear. To investigate this, we used single-cell RNA sequencing to quantify transcript heterogeneity in single Saccharomyces cerevisiae cells treated with...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.2004050

    authors： Gasch AP,Yu FB,Hose J,Escalante LE,Place M,Bacher R,Kanbar J,Ciobanu D,Sandor L,Grigoriev IV,Kendziorski C,Quake SR,McClean MN

    更新日期：2017-12-14 00:00:00

  • Functional diversity of plant-pollinator interaction webs enhances the persistence of plant communities.

    abstract：:Pollination is exclusively or mainly animal mediated for 70% to 90% of angiosperm species. Thus, pollinators provide an essential ecosystem service to humankind. However, the impact of human-induced biodiversity loss on the functioning of plant-pollinator interactions has not been tested experimentally. To understand ...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.0040001

    authors： Fontaine C,Dajoz I,Meriguet J,Loreau M

    更新日期：2006-01-01 00:00:00

  • EpCAM ectodomain EpEX is a ligand of EGFR that counteracts EGF-mediated epithelial-mesenchymal transition through modulation of phospho-ERK1/2 in head and neck cancers.

    abstract：:Head and neck squamous cell carcinomas (HNSCCs) are characterized by outstanding molecular heterogeneity that results in severe therapy resistance and poor clinical outcome. Inter- and intratumoral heterogeneity in epithelial-mesenchymal transition (EMT) was recently revealed as a major parameter of poor clinical outc...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.2006624

    authors： Pan M,Schinke H,Luxenburger E,Kranz G,Shakhtour J,Libl D,Huang Y,Gaber A,Pavšič M,Lenarčič B,Kitz J,Jakob M,Schwenk-Zieger S,Canis M,Hess J,Unger K,Baumeister P,Gires O

    更新日期：2018-09-27 00:00:00

  • CD4 occupancy triggers sequential pre-fusion conformational states of the HIV-1 envelope trimer with relevance for broadly neutralizing antibody activity.

    abstract：:During the entry process, the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trimer undergoes a sequence of conformational changes triggered by both CD4 and coreceptor engagement. Resolving the conformation of these transient entry intermediates has proven challenging. Here, we fine-mapped the...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.3000114

    authors： Ivan B,Sun Z,Subbaraman H,Friedrich N,Trkola A

    更新日期：2019-01-16 00:00:00

  • MamO Is a Repurposed Serine Protease that Promotes Magnetite Biomineralization through Direct Transition Metal Binding in Magnetotactic Bacteria.

    abstract：:Many living organisms transform inorganic atoms into highly ordered crystalline materials. An elegant example of such biomineralization processes is the production of nano-scale magnetic crystals in magnetotactic bacteria. Previous studies implicated the involvement of two putative serine proteases, MamE and MamO, dur...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.1002402

    authors： Hershey DM,Ren X,Melnyk RA,Browne PJ,Ozyamak E,Jones SR,Chang MC,Hurley JH,Komeili A

    更新日期：2016-03-16 00:00:00

  • Performing group-level functional image analyses based on homologous functional regions mapped in individuals.

    abstract：:Functional MRI (fMRI) studies have traditionally relied on intersubject normalization based on global brain morphology, which cannot establish proper functional correspondence between subjects due to substantial intersubject variability in functional organization. Here, we reliably identified a set of discrete, homolo...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.2007032

    authors： Li M,Wang D,Ren J,Langs G,Stoecklein S,Brennan BP,Lu J,Chen H,Liu H

    更新日期：2019-03-25 00:00:00

  • Strength in numbers: Collaborative science for new experimental model systems.

    abstract：:Our current understanding of biology is heavily based on a small number of genetically tractable model organisms. Most eukaryotic phyla lack such experimental models, and this limits our ability to explore the molecular mechanisms that ultimately define their biology, ecology, and diversity. In particular, marine prot...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.2006333

    authors： Waller RF,Cleves PA,Rubio-Brotons M,Woods A,Bender SJ,Edgcomb V,Gann ER,Jones AC,Teytelman L,von Dassow P,Wilhelm SW,Collier JL

    更新日期：2018-07-02 00:00:00

  • Revisiting the basic reproductive number for malaria and its implications for malaria control.

    abstract：:The prospects for the success of malaria control depend, in part, on the basic reproductive number for malaria, R0. Here, we estimate R0 in a novel way for 121 African populations, and thereby increase the number of R0 estimates for malaria by an order of magnitude. The estimates range from around one to more than 3,0...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.0050042

    authors： Smith DL,McKenzie FE,Snow RW,Hay SI

    更新日期：2007-03-01 00:00:00

  • Building a local community of practice in scientific programming for life scientists.

    abstract：:In this paper, we describe why and how to build a local community of practice in scientific programming for life scientists who use computers and programming in their research. A community of practice is a small group of scientists who meet regularly to help each other and promote good practices in scientific programm...

    journal_title：PLoS biology

    pub_type： 杂志文章

    doi：10.1371/journal.pbio.2005561

    authors： Stevens SLR,Kuzak M,Martinez C,Moser A,Bleeker P,Galland M

    更新日期：2018-11-28 00:00:00