Abstract:
:The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Korkhin Y,Unligil UM,Littlefield O,Nelson PJ,Stuart DI,Sigler PB,Bell SD,Abrescia NGdoi
10.1371/journal.pbio.1000102subject
Has Abstractpub_date
2009-05-01 00:00:00pages
e1000102issue
5eissn
1544-9173issn
1545-7885pii
08-PLBI-RA-4468journal_volume
7pub_type
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