Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication.

Abstract:

:Cobalamin-independent methionine synthase (MetE) catalyzes the transfer of a methyl group from methyltetrahydrofolate to L-homocysteine (Hcy) without using an intermediate methyl carrier. Although MetE displays no detectable sequence homology with cobalamin-dependent methionine synthase (MetH), both enzymes require zinc for activation and binding of Hcy. Crystallographic analyses of MetE from T. maritima reveal an unusual dual-barrel structure in which the active site lies between the tops of the two (betaalpha)(8) barrels. The fold of the N-terminal barrel confirms that it has evolved from the C-terminal polypeptide by gene duplication; comparisons of the barrels provide an intriguing example of homologous domain evolution in which binding sites are obliterated. The C-terminal barrel incorporates the zinc ion that binds and activates Hcy. The zinc-binding site in MetE is distinguished from the (Cys)(3)Zn site in the related enzymes, MetH and betaine-homocysteine methyltransferase, by its position in the barrel and by the metal ligands, which are histidine, cysteine, glutamate, and cysteine in the resting form of MetE. Hcy associates at the face of the metal opposite glutamate, which moves away from the zinc in the binary E.Hcy complex. The folate substrate is not intimately associated with the N-terminal barrel; instead, elements from both barrels contribute binding determinants in a binary complex in which the folate substrate is incorrectly oriented for methyl transfer. Atypical locations of the Hcy and folate sites in the C-terminal barrel presumably permit direct interaction of the substrates in a ternary complex. Structures of the binary substrate complexes imply that rearrangement of folate, perhaps accompanied by domain rearrangement, must occur before formation of a ternary complex that is competent for methyl transfer.

journal_name

PLoS Biol

journal_title

PLoS biology

authors

Pejchal R,Ludwig ML

doi

10.1371/journal.pbio.0030031

keywords:

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

e31

issue

2

eissn

1544-9173

issn

1545-7885

journal_volume

3

pub_type

杂志文章
  • Neuroinflammation in the normal-appearing white matter (NAWM) of the multiple sclerosis brain causes abnormalities at the nodes of Ranvier.

    abstract::Changes to the structure of nodes of Ranvier in the normal-appearing white matter (NAWM) of multiple sclerosis (MS) brains are associated with chronic inflammation. We show that the paranodal domains in MS NAWM are longer on average than control, with Kv1.2 channels dislocated into the paranode. These pathological fea...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3001008

    authors: Gallego-Delgado P,James R,Browne E,Meng J,Umashankar S,Tan L,Picon C,Mazarakis ND,Faisal AA,Howell OW,Reynolds R

    更新日期:2020-12-14 00:00:00

  • CEP290 is essential for the initiation of ciliary transition zone assembly.

    abstract::Cilia play critical roles during embryonic development and adult homeostasis. Dysfunction of cilia leads to various human genetic diseases, including many caused by defects in transition zones (TZs), the "gates" of cilia. The evolutionarily conserved TZ component centrosomal protein 290 (CEP290) is the most frequently...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3001034

    authors: Wu Z,Pang N,Zhang Y,Chen H,Peng Y,Fu J,Wei Q

    更新日期:2020-12-28 00:00:00

  • Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain.

    abstract::Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a key role in regulating the levels of plasma low-density lipoprotein cholesterol (LDL-C). Here, we demonstrate that the compound PF-06446846 inhibits translation of PCSK9 by inducing the ribosome to stall around codon 34, mediated by the sequence of the nasc...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.2001882

    authors: Lintner NG,McClure KF,Petersen D,Londregan AT,Piotrowski DW,Wei L,Xiao J,Bolt M,Loria PM,Maguire B,Geoghegan KF,Huang A,Rolph T,Liras S,Doudna JA,Dullea RG,Cate JH

    更新日期:2017-03-21 00:00:00

  • Networking in the Plant Microbiome.

    abstract::Almost all higher organisms, including plants, insects, and mammals, are colonized by complex microbial communities and harbor a microbiome. Emerging studies with plants reveal that these microbiomes are structured and form complex, interconnected microbial networks. Within these networks, different taxa have differen...

    journal_title:PLoS biology

    pub_type: 评论,杂志文章

    doi:10.1371/journal.pbio.1002378

    authors: van der Heijden MG,Hartmann M

    更新日期:2016-02-12 00:00:00

  • Determination of stromal signatures in breast carcinoma.

    abstract::Many soft tissue tumors recapitulate features of normal connective tissue. We hypothesize that different types of fibroblastic tumors are representative of different populations of fibroblastic cells or different activation states of these cells. We examined two tumors with fibroblastic features, solitary fibrous tumo...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0030187

    authors: West RB,Nuyten DS,Subramanian S,Nielsen TO,Corless CL,Rubin BP,Montgomery K,Zhu S,Patel R,Hernandez-Boussard T,Goldblum JR,Brown PO,van de Vijver M,van de Rijn M

    更新日期:2005-06-01 00:00:00

  • Harvesting the promising fruits of genomics: applying genome sequencing technologies to crop breeding.

    abstract::Next generation sequencing (NGS) technologies are being used to generate whole genome sequences for a wide range of crop species. When combined with precise phenotyping methods, these technologies provide a powerful and rapid tool for identifying the genetic basis of agriculturally important traits and for predicting ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001883

    authors: Varshney RK,Terauchi R,McCouch SR

    更新日期:2014-06-10 00:00:00

  • DEAD-box helicase proteins disrupt RNA tertiary structure through helix capture.

    abstract::DEAD-box helicase proteins accelerate folding and rearrangements of highly structured RNAs and RNA-protein complexes (RNPs) in many essential cellular processes. Although DEAD-box proteins have been shown to use ATP to unwind short RNA helices, it is not known how they disrupt RNA tertiary structure. Here, we use sing...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001981

    authors: Pan C,Potratz JP,Cannon B,Simpson ZB,Ziehr JL,Tijerina P,Russell R

    更新日期:2014-10-28 00:00:00

  • Science incubators: synthesis centers and their role in the research ecosystem.

    abstract::How should funding agencies enable researchers to explore high-risk but potentially high-reward science? One model that appears to work is the NSF-funded synthesis center, an incubator for community-led, innovative science. ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001468

    authors: Rodrigo A,Alberts S,Cranston K,Kingsolver J,Lapp H,McClain C,Smith R,Vision T,Weintraub J,Wiegmann B

    更新日期:2013-01-01 00:00:00

  • The slings and arrows of comparative linguistics.

    abstract::In this Formal Comment the authors respond to objections to their previous Essay, reiterating that comparative linguistics is not an easy undertaking. ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000019

    authors: Bolhuis JJ,Beckers GJL,Huybregts MAC,Berwick RC,Everaert MBH

    更新日期:2018-09-24 00:00:00

  • Functional dissection of an innate immune response by a genome-wide RNAi screen.

    abstract::The innate immune system is ancient and highly conserved. It is the first line of defense and the only recognizable immune system in the vast majority of metazoans. Signaling events that convert pathogen detection into a defense response are central to innate immunity. Drosophila has emerged as an invaluable model org...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0020203

    authors: Foley E,O'Farrell PH

    更新日期:2004-08-01 00:00:00

  • Strength in numbers: Collaborative science for new experimental model systems.

    abstract::Our current understanding of biology is heavily based on a small number of genetically tractable model organisms. Most eukaryotic phyla lack such experimental models, and this limits our ability to explore the molecular mechanisms that ultimately define their biology, ecology, and diversity. In particular, marine prot...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.2006333

    authors: Waller RF,Cleves PA,Rubio-Brotons M,Woods A,Bender SJ,Edgcomb V,Gann ER,Jones AC,Teytelman L,von Dassow P,Wilhelm SW,Collier JL

    更新日期:2018-07-02 00:00:00

  • Structural studies of the giant mimivirus.

    abstract::Mimivirus is the largest known virus whose genome and physical size are comparable to some small bacteria, blurring the boundary between a virus and a cell. Structural studies of Mimivirus have been difficult because of its size and long surface fibers. Here we report the use of enzymatic digestions to remove the surf...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000092

    authors: Xiao C,Kuznetsov YG,Sun S,Hafenstein SL,Kostyuchenko VA,Chipman PR,Suzan-Monti M,Raoult D,McPherson A,Rossmann MG

    更新日期:2009-04-28 00:00:00

  • Pancreatic mesenchyme regulates epithelial organogenesis throughout development.

    abstract::The developing pancreatic epithelium gives rise to all endocrine and exocrine cells of the mature organ. During organogenesis, the epithelial cells receive essential signals from the overlying mesenchyme. Previous studies, focusing on ex vivo tissue explants or complete knockout mice, have identified an important role...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001143

    authors: Landsman L,Nijagal A,Whitchurch TJ,Vanderlaan RL,Zimmer WE,Mackenzie TC,Hebrok M

    更新日期:2011-09-01 00:00:00

  • Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing.

    abstract::5-hydroxymethylcytosine (5hmC) is the most prevalent intermediate on the oxidative DNA demethylation pathway and is implicated in regulation of embryogenesis, neurological processes, and cancerogenesis. Profiling of this relatively scarce genomic modification in clinical samples requires cost-effective high-resolution...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000684

    authors: Gibas P,Narmontė M,Staševskij Z,Gordevičius J,Klimašauskas S,Kriukienė E

    更新日期:2020-04-10 00:00:00

  • Cdk5 regulates accurate maturation of newborn granule cells in the adult hippocampus.

    abstract::Newborn granule cells become functionally integrated into the synaptic circuitry of the adult dentate gyrus after a morphological and electrophysiological maturation process. The molecular mechanisms by which immature neurons and the neurites extending from them find their appropriate position and target area remain l...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0060272

    authors: Jessberger S,Aigner S,Clemenson GD Jr,Toni N,Lie DC,Karalay O,Overall R,Kempermann G,Gage FH

    更新日期:2008-11-11 00:00:00

  • VirE2: a unique ssDNA-compacting molecular machine.

    abstract::The translocation of single-stranded DNA (ssDNA) across membranes of two cells is a fundamental biological process occurring in both bacterial conjugation and Agrobacterium pathogenesis. Whereas bacterial conjugation spreads antibiotic resistance, Agrobacterium facilitates efficient interkingdom transfer of ssDNA from...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0060044

    authors: Grange W,Duckely M,Husale S,Jacob S,Engel A,Hegner M

    更新日期:2008-02-01 00:00:00

  • Drosophila melanogaster establishes a species-specific mutualistic interaction with stable gut-colonizing bacteria.

    abstract::Animals live together with diverse bacteria that can impact their biology. In Drosophila melanogaster, gut-associated bacterial communities are relatively simple in composition but also have a strong impact on host development and physiology. It is generally assumed that gut bacteria in D. melanogaster are transient a...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.2005710

    authors: Pais IS,Valente RS,Sporniak M,Teixeira L

    更新日期:2018-07-05 00:00:00

  • Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.

    abstract::The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfol...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000102

    authors: Korkhin Y,Unligil UM,Littlefield O,Nelson PJ,Stuart DI,Sigler PB,Bell SD,Abrescia NG

    更新日期:2009-05-01 00:00:00

  • Correction: Novel Role of NOX in Supporting Aerobic Glycolysis in Cancer Cells with Mitochondrial Dysfunction and as a Potential Target for Cancer Therapy.

    abstract::[This corrects the article DOI: 10.1371/journal.pbio.1001326.]. ...

    journal_title:PLoS biology

    pub_type: 杂志文章,已发布勘误

    doi:10.1371/journal.pbio.1002616

    authors: Lu W,Hu Y,Chen G,Chen Z,Zhang H,Wang F,Feng L,Pelicano H,Wang H,Keating MJ,Liu J,McKeehan W,Wang H,Luo Y,Huang P

    更新日期:2017-12-11 00:00:00

  • CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.

    abstract::We show that the cyclin-dependent kinase inhibitor 1B (CDKN1B)/p27, previously known as a cell cycle inhibitor, is also localized within mitochondria. The migratory capacity of endothelial cells, which need intact mitochondria, is completely dependent on mitochondrial p27. Mitochondrial p27 improves mitochondrial memb...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.2004408

    authors: Ale-Agha N,Goy C,Jakobs P,Spyridopoulos I,Gonnissen S,Dyballa-Rukes N,Aufenvenne K,von Ameln F,Zurek M,Spannbrucker T,Eckermann O,Jakob S,Gorressen S,Abrams M,Grandoch M,Fischer JW,Köhrer K,Deenen R,Unfried K,Altsch

    更新日期:2018-06-21 00:00:00

  • A systematic approach to pair secretory cargo receptors with their cargo suggests a mechanism for cargo selection by Erv14.

    abstract::The endoplasmic reticulum (ER) is the site of synthesis of secreted and membrane proteins. To exit the ER, proteins are packaged into COPII vesicles through direct interaction with the COPII coat or aided by specific cargo receptors. Despite the fundamental role of such cargo receptors in protein traffic, only a few h...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001329

    authors: Herzig Y,Sharpe HJ,Elbaz Y,Munro S,Schuldiner M

    更新日期:2012-01-01 00:00:00

  • Control over single-cell distribution of G1 lengths by WNT governs pluripotency.

    abstract::The link between single-cell variation and population-level fate choices lacks a mechanistic explanation despite extensive observations of gene expression and epigenetic variation among individual cells. Here, we found that single human embryonic stem cells (hESCs) have different and biased differentiation potentials ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000453

    authors: Jang J,Han D,Golkaram M,Audouard M,Liu G,Bridges D,Hellander S,Chialastri A,Dey SS,Petzold LR,Kosik KS

    更新日期:2019-09-26 00:00:00

  • Multi-scale in vivo systems analysis reveals the influence of immune cells on TNF-α-induced apoptosis in the intestinal epithelium.

    abstract::Intestinal epithelial cells exist within a complex environment that affects how they interpret and respond to stimuli. We have applied a multi-scale in vivo systems approach to understand how intestinal immune cells communicate with epithelial cells to regulate responses to inflammatory signals. Multivariate modeling ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001393

    authors: Lau KS,Cortez-Retamozo V,Philips SR,Pittet MJ,Lauffenburger DA,Haigis KM

    更新日期:2012-01-01 00:00:00

  • Diverse modes of axon elaboration in the developing neocortex.

    abstract::The development of axonal arbors is a critical step in the establishment of precise neural circuits, but relatively little is known about the mechanisms of axonal elaboration in the neocortex. We used in vivo two-photon time-lapse microscopy to image axons in the neocortex of green fluorescent protein-transgenic mice ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0030272

    authors: Portera-Cailliau C,Weimer RM,De Paola V,Caroni P,Svoboda K

    更新日期:2005-08-01 00:00:00

  • A Src-like inactive conformation in the abl tyrosine kinase domain.

    abstract::The improper activation of the Abl tyrosine kinase results in chronic myeloid leukemia (CML). The recognition of an inactive conformation of Abl, in which a catalytically important Asp-Phe-Gly (DFG) motif is flipped by approximately 180 degrees with respect to the active conformation, underlies the specificity of the ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0040144

    authors: Levinson NM,Kuchment O,Shen K,Young MA,Koldobskiy M,Karplus M,Cole PA,Kuriyan J

    更新日期:2006-05-01 00:00:00

  • Munc18-bound syntaxin readily forms SNARE complexes with synaptobrevin in native plasma membranes.

    abstract::Munc18-1, a protein essential for regulated exocytosis in neurons and neuroendocrine cells, belongs to the family of Sec1/Munc18-like (SM) proteins. In vitro, Munc18-1 forms a tight complex with the SNARE syntaxin 1, in which syntaxin is stabilized in a closed conformation. Since closed syntaxin is unable to interact ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0040330

    authors: Zilly FE,Sørensen JB,Jahn R,Lang T

    更新日期:2006-10-01 00:00:00

  • Assessing evidence for a pervasive alteration in tropical tree communities.

    abstract::In Amazonian tropical forests, recent studies have reported increases in aboveground biomass and in primary productivity, as well as shifts in plant species composition favouring fast-growing species over slow-growing ones. This pervasive alteration of mature tropical forests was attributed to global environmental cha...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0060045

    authors: Chave J,Condit R,Muller-Landau HC,Thomas SC,Ashton PS,Bunyavejchewin S,Co LL,Dattaraja HS,Davies SJ,Esufali S,Ewango CE,Feeley KJ,Foster RB,Gunatilleke N,Gunatilleke S,Hall P,Hart TB,Hernández C,Hubbell SP,Itoh A,

    更新日期:2008-03-04 00:00:00

  • Polarised asymmetric inheritance of accumulated protein damage in higher eukaryotes.

    abstract::Disease-associated misfolded proteins or proteins damaged due to cellular stress are generally disposed via the cellular protein quality-control system. However, under saturating conditions, misfolded proteins will aggregate. In higher eukaryotes, these aggregates can be transported to accumulate in aggresomes at the ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0040417

    authors: Rujano MA,Bosveld F,Salomons FA,Dijk F,van Waarde MA,van der Want JJ,de Vos RA,Brunt ER,Sibon OC,Kampinga HH

    更新日期:2006-12-01 00:00:00

  • Peli1 impairs microglial Aβ phagocytosis through promoting C/EBPβ degradation.

    abstract::Amyloid-β (Aβ) accumulation in the brain is a hallmark of Alzheimer's disease (AD) pathology. However, the molecular mechanism controlling microglial Aβ phagocytosis is poorly understood. Here we found that the E3 ubiquitin ligase Pellino 1 (Peli1) is induced in the microglia of AD-like five familial AD (5×FAD) mice, ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000837

    authors: Xu J,Yu T,Pietronigro EC,Yuan J,Arioli J,Pei Y,Luo X,Ye J,Constantin G,Mao C,Xiao Y

    更新日期:2020-10-05 00:00:00

  • Influence of dopaminergically mediated reward on somatosensory decision-making.

    abstract:UNLABELLED:Reward-related dopaminergic influences on learning and overt behaviour are well established, but any influence on sensory decision-making is largely unknown. We used functional magnetic resonance imaging (fMRI) while participants judged electric somatosensory stimuli on one hand or other, before being reward...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000164

    authors: Pleger B,Ruff CC,Blankenburg F,Klöppel S,Driver J,Dolan RJ

    更新日期:2009-07-01 00:00:00