Abstract:
:The GAL4 protein activates transcription of the genes required for galactose utilization in Saccharomyces cerevisiae. The protein, consisting of 881 amino acids, is dimeric when bound to one of the approximately twofold symmetrical DNA sites present in the galactose upstream activating sequence (UASG). Here we use two-dimensional NMR spectroscopy to determine the structure of an amino-terminal fragment of GAL4 (residues 1-65). This fragment, a monomer in solution, binds as a dimer specifically to UASG-containing DNA. Residues 9-40 form a well defined, compact globular cluster, whereas residues 1-8 and 41-66 show considerable conformational mobility in the absence of DNA. The compact domain contains a motif in which six cysteines, located on two symmetrically related helix/extended strand units connected by a long loop, coordinate two central zinc ions, forming a bimetal-thiolate cluster. The zincs were replaced by NMR-active 113Cd in most of our work and structural parameters are therefore derived from the Cd2-protein. The structure obtained for the GAL4 DNA-binding domain represents a novel DNA-binding motif. Essentially the same conformation is observed for the compact domain in solution using NMR techniques as was seen for the central core of the N-terminal fragment bound to DNA using crystallographic techniques. Thus, the core of the DNA-binding domain changes little upon binding DNA.
journal_name
Naturejournal_title
Natureauthors
Baleja JD,Marmorstein R,Harrison SC,Wagner Gdoi
10.1038/356450a0keywords:
subject
Has Abstractpub_date
1992-04-02 00:00:00pages
450-3issue
6368eissn
0028-0836issn
1476-4687journal_volume
356pub_type
杂志文章相关文献
NATURE文献大全abstract::The products of six unidentified reading frames of human mitochondrial DNA are precipitated from a mitochondrial lysate by antibodies against highly purified native beef heart NADH-ubiquinone oxidoreductase (complex I). These products are enriched greatly in a human submitochondrial fraction enriched in NADH-Q1 and NA...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/314592a0
更新日期:1985-04-18 00:00:00
abstract::Large-conductance Ca(2+)-gated K(+) (BK) channels are essential for many biological processes such as smooth muscle contraction and neurotransmitter release. This group of channels can be activated synergistically by both voltage and intracellular Ca(2+), with the large carboxy-terminal intracellular portion being res...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09252
更新日期:2010-07-15 00:00:00
abstract::Cells operate in dynamic environments using extraordinary communication capabilities that emerge from the interactions of genetic circuitry. The mammalian immune response is a striking example of the coordination of different cell types. Cell-to-cell communication is primarily mediated by signalling molecules that for...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09145
更新日期:2010-07-08 00:00:00
abstract::Throughout the lifespan of a plant, which in some cases can last more than one thousand years, the stem cell niches in the root and shoot apical meristems provide cells for the formation of complete root and shoot systems, respectively. Both niches are superficially different and it has remained unclear whether common...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05703
更新日期:2007-04-12 00:00:00
abstract::Haploids and double haploids are important resources for studying recessive traits and have large impacts on crop breeding, but natural haploids are rare in animals. Mammalian haploids are restricted to germline cells and are occasionally found in tumours with massive chromosome loss. Recent success in establishing ha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11435
更新日期:2012-10-18 00:00:00
abstract::Mutations in the Schizosaccharomyces pombe cdc8 gene impair cytokinesis. Here we clone cdc8+ and find that it encodes a novel tropomyosin. Gene disruption results in lethal arrest of the cell cycle, but spore germination, cell growth, DNA replication and mitosis are all unaffected. Haploid cdc8 gene disruptants are re...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/360084a0
更新日期:1992-11-05 00:00:00
abstract::Corticosteroids as well as non-steroid anti-inflammatory drugs inhibit the prostaglandin-mediated vasodilatation accompanying lipolysis in subcutaneous fat. Whereas the non-steroids produce their effect by inhibition of prostaglandin synthesis, however, corticosteroids inhibit their release. This mechanism may be the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/254308a0
更新日期:1975-03-27 00:00:00
abstract::Clear cell renal cell carcinoma (ccRCC), the most common form of kidney cancer, is characterized by elevated glycogen levels and fat deposition. These consistent metabolic alterations are associated with normoxic stabilization of hypoxia-inducible factors (HIFs) secondary to von Hippel-Lindau (VHL) mutations that occu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13557
更新日期:2014-09-11 00:00:00
abstract::The upright posture and obligatory bipedalism of modern humans are unique among living primates. The evolutionary history of this behaviour has traditionally been pursued by functional analysis of the postcranial skeleton and the preserved footprint trails of fossil hominids. Here we report a systematic attempt to rec...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/369645a0
更新日期:1994-06-23 00:00:00
abstract::Differentiation of bone marrow derived precursors into mature T cells takes place in the thymus. During differentiation, T cells develop the receptor repertoire which allows them to recognize antigen in the context of self major histocompatibility complex (MHC) molecules. Mature T helper cells (mostly CD4+ CD8-) recog...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/333180a0
更新日期:1988-05-12 00:00:00
abstract::Reported widespread declines of wild and managed insect pollinators have serious consequences for global ecosystem services and agricultural production. Bees contribute approximately 80% of insect pollination, so it is important to understand and mitigate the causes of current declines in bee populations . Recent stud...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11585
更新日期:2012-11-01 00:00:00
abstract::Only a very small fraction of long noncoding RNAs (lncRNAs) are well characterized. The evolutionary history of lncRNAs can provide insights into their functionality, but the absence of lncRNA annotations in non-model organisms has precluded comparative analyses. Here we present a large-scale evolutionary study of lnc...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12943
更新日期:2014-01-30 00:00:00
abstract::It is generally thought that interspecific (lateral) transfer of genes is so extensive among bacteria that it is difficult, and perhaps impossible, to determine their phylogenetic relationships. Ambler and coworkers reflect this in their suggestion that the relationships seen among cytochrome c sequences of the Rhodos...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/283212a0
更新日期:1980-01-10 00:00:00
abstract::There is growing recognition that mammalian cells produce many thousands of large intergenic transcripts. However, the functional significance of these transcripts has been particularly controversial. Although there are some well-characterized examples, most (>95%) show little evidence of evolutionary conservation and...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07672
更新日期:2009-03-12 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/d41586-019-00752-3
更新日期:2019-03-01 00:00:00
abstract::Volcanic eruptions contribute to climate variability, but quantifying these contributions has been limited by inconsistencies in the timing of atmospheric volcanic aerosol loading determined from ice cores and subsequent cooling from climate proxies such as tree rings. Here we resolve these inconsistencies and show th...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature14565
更新日期:2015-07-30 00:00:00
abstract::The gut microbiota influences development1-3 and homeostasis4-7 of the mammalian immune system, and is associated with human inflammatory8 and immune diseases9,10 as well as responses to immunotherapy11-14. Nevertheless, our understanding of how gut bacteria modulate the immune system remains limited, particularly in ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2971-8
更新日期:2020-12-01 00:00:00
abstract::We have shown previously that chromosome VI of Saccharomyces cerevisiae contains nine origins of DNA replication that differ in initiation frequency and replicate sequentially during the S phase of the cell cycle. Here we show that there are links between activation of these multiple origins and regulation of S-phase ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/27007
更新日期:1998-10-08 00:00:00
abstract::Supersolids are exotic materials combining the frictionless flow of a superfluid with the crystal-like periodic density modulation of a solid. The supersolid phase of matter was predicted 50 years ago1-3 for solid helium4-8. Ultracold quantum gases have recently been made to exhibit periodic order typical of a crystal...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1568-6
更新日期:2019-10-01 00:00:00
abstract::The UK Farm-Scale Evaluations (FSE) compared the effects on biodiversity of management of genetically modified herbicide-tolerant (GMHT) spring-sown crops with conventional crop management. The FSE reported larger weed abundance under GMHT management for fodder maize, one of three crops studied. Increased seed product...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02374
更新日期:2004-03-18 00:00:00
abstract::Remodelling its shape, or morphogenesis, is a fundamental property of living tissue. It underlies much of embryonic development and numerous pathologies. Convergent extension (CE) of the axial mesoderm of vertebrates is an intensively studied model for morphogenetic processes that rely on cell rearrangement. It involv...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02620
更新日期:2004-07-15 00:00:00
abstract::The function and nature of inhibition of neurons in the visual cortex have been the focus of both experimental and theoretical investigations. There are two ways in which inhibition can suppress synaptic excitation. In hyperpolarizing inhibition, negative and positive currents sum linearly to produce a net change in m...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/30735
更新日期:1998-05-28 00:00:00
abstract::Bottom trawling is a non-selective commercial fishing technique whereby heavy nets and gear are pulled along the sea floor. The direct impact of this technique on fish populations and benthic communities has received much attention, but trawling can also modify the physical properties of seafloor sediments, water–sedi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11410
更新日期:2012-09-13 00:00:00
abstract::UV light is a potent mutagen in most living cells, but molecular analysis of its mode of action in animal cells has not been possible because of the high complexity of the cell genome. Therefore, we have used simian virus 40 (SV40) as a biological probe to study the mutagenic effect of UV-irradiation at the molecular ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/305068a0
更新日期:1983-09-01 00:00:00
abstract::To ensure cell survival, it is essential that the ubiquitous pro-apoptotic machinery is kept quiescent. As death is irreversible, cells must continually integrate developmental information with regulatory inputs to control the switch between repressing and activating apoptosis. Inappropriate activation or suppression ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01920
更新日期:2003-08-28 00:00:00
abstract::Transgenic mice bearing the cellular myc oncogene coupled to the immunoglobulin mu or kappa enhancer frequently develop a fatal lymphoma within a few months of birth. Since the tumours represent represent both immature and mature B lymphocytes, constitutive c-myc expression appears to be highly leukaemogenic at severa...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/318533a0
更新日期:1985-12-12 00:00:00
abstract::Protein modification by the conjugation of ubiquitin moieties--ubiquitination--plays a major part in many biological processes, including cell cycle and apoptosis. The enzymes that mediate ubiquitin-conjugation have been well-studied, but much less is known about the ubiquitin-specific proteases that mediate de-ubiqui...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01811
更新日期:2003-08-14 00:00:00
abstract::Cyclosporin A (CSA) is a fungal metabolite which exerts profound effects on the immune system and has potential as a selective immuno-suppressive agent. Clinical trials with human renal allograft recipients have confirmed this potential but there have been disturbing reports of lymphoma in a significant number of pati...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/289300a0
更新日期:1981-01-22 00:00:00
abstract::The bmi-1 proto-oncogene can be activated by Moloney murine leukaemia proviral insertions in E mu-myc transgenic mice. It encodes a highly conserved nuclear protein of 324 amino acids which belongs to a family of proteins containing a putative new zinc-finger. Another closely related member of this family is the mouse...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/353353a0
更新日期:1991-09-26 00:00:00
abstract::HIV-1 immunotherapy with a combination of first generation monoclonal antibodies was largely ineffective in pre-clinical and clinical settings and was therefore abandoned. However, recently developed single-cell-based antibody cloning methods have uncovered a new generation of far more potent broadly neutralizing anti...
journal_title:Nature
pub_type: 杂志文章,多中心研究
doi:10.1038/nature14411
更新日期:2015-06-25 00:00:00