当前位置： SCI文献检索 > IMMUNOLOGY期刊下所有文献 > Heterogeneous expression of apolipoprotein-E by human macrophages.

Heterogeneous expression of apolipoprotein-E by human macrophages.

Abstract：

:Apolipoprotein-E (apoE) is expressed at high levels by macrophages. In addition to its role in lipid transport, macrophage-derived apoE plays an important role in immunoregulation. Previous studies have identified macrophage subpopulations that differ substantially in their ability to synthesize specific cytokines and enzymes, however, potential heterogeneous macrophage apoE expression has not been studied. Here we examined apoE expression in human THP-1 macrophages and monocyte-derived macrophages (MDM). Using immunocytochemistry and flow cytometry methods we reveal a striking heterogeneity in macrophage apoE expression in both cell types. In phorbol-ester-differentiated THP-1 macrophages, 5% of the cells over-expressed apoE at levels more than 50-fold higher than the rest of the population. ApoE over-expressing THP-1 macrophages contained condensed/fragmented nuclei and increased levels of activated caspase-3 indicating induction of apoptosis. In MDM, 3-5% of the cells also highly over-expressed apoE, up to 50-fold higher than the rest of the population; however, this was not associated with obvious nuclear alterations. The apoE over-expressing MDM were larger, more granular, and more autofluorescent than the majority of cells and they contained numerous vesicle-like structures that appeared to be coated by apoE. Flow cytometry experiments indicated that the apoE over-expressing subpopulation of MDM were positive for CD14, CD11b/Mac-1 and CD68. These observations suggest that specific macrophage subpopulations may be important for apoE-mediated immunoregulation and clearly indicate that subpopulation heterogeneity should be taken into account when investigating macrophage apoE expression.

journal_name

Immunology

journal_title

Immunology

authors

Tedla N,Glaros EN,Brunk UT,Jessup W,Garner B

doi

10.1111/j.1365-2567.2004.01972.x

keywords:

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

338-47

issue

3

eissn

0019-2805

issn

1365-2567

pii

IMM1972

journal_volume

113

pub_type

杂志文章

相关文献

IMMUNOLOGY文献大全
  • Distinct activation phenotype of a highly conserved novel HLA-B57-restricted epitope during dengue virus infection.

    abstract：:Variation in the sequence of T-cell epitopes between dengue virus (DENV) serotypes is believed to alter memory T-cell responses during second heterologous infections. We identified a highly conserved, novel, HLA-B57-restricted epitope on the DENV NS1 protein. We predicted higher frequencies of B57-NS1(26-34) -specific...

    journal_title：Immunology

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1111/imm.12161

    authors： Townsley E,Woda M,Thomas SJ,Kalayanarooj S,Gibbons RV,Nisalak A,Srikiatkhachorn A,Green S,Stephens HA,Rothman AL,Mathew A

    更新日期：2014-01-01 00:00:00

  • Enhancement of immunogenicity of tumour virus antigen by liposomes: the effect of lipid composition.

    abstract：:The secondary humoral response evoked in W/Fu rats by the weakly immunogenic soluble Gross cell surface antigen (GCSAa) extracted from the syngeneic (C58NT)D lymphoma can be enhanced when GCSAa is presented in liposomes, and this requires the antigen to be strongly associated with the phospholipid bilayers. In order t...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Bakouche O,Gerlier D

    更新日期：1986-07-01 00:00:00

  • T-cells expressing a chimeric-PD1-Dap10-CD3zeta receptor reduce tumour burden in multiple murine syngeneic models of solid cancer.

    abstract：:Adoptive transfer of T-cells is a promising therapy for many cancers. To enhance tumour recognition by T-cells, chimeric antigen receptors (CARs) consisting of signalling domains fused to receptors that recognize tumour-associated antigens can be expressed in T-cells. While CAR T-cells have shown clinical success for ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.13187

    authors： Parriott G,Deal K,Crean S,Richardson E,Nylen E,Barber A

    更新日期：2020-07-01 00:00:00

  • Analysis of the mechanism of immunodepression following heterologous antigenic stimulation during concurrent infection with Nematospiroides dubius.

    abstract：:The suppression of immune responsiveness to heterologous antigenic stimulation during concurrent infection with Nematospiroides dubius was reproduced using soluble antigens derived from adult parasites. Immunosuppression appeared to be selective in that the administration of equivalent quantities of an irrelevant hete...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Pritchard DI,Ali NM,Behnke JM

    更新日期：1984-04-01 00:00:00

  • Study of blood group B antigen with a specific monoclonal antibody (anti-B, b-183).

    abstract：:A murine anti-B monoclonal antibody was obtained by the hybridoma technique. This antibody called anti-B (b-183) is of IgM nature; it is capable of agglutinating normal B, B3, Bx, cis AB and some acquired B red cells. Its association constant is 1.1 X 10(8) l/mol, and appears high compared to those of the monoclonal a...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Rouger P,Edelman L,Doinel C,Reviron J,Salmon C,Bach JF

    更新日期：1983-05-01 00:00:00

  • Association of human leucocyte low responsiveness to inducers of interferon alpha with HLA-DR 2.

    abstract：:The influence of antigens of the major histocompatibility complex (MHC) on the production of interferon (IFN) alpha or IFN gamma by human peripheral blood leucocytes (PBL) in vitro has been studied. Synthesis of IFN gamma by PBL stimulated with purified phytohaemagglutinin (PHA-P) or protein A of Staphylococcus aureus...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Abb J,Zander H,Abb H,Albert E,Deinhardt F

    更新日期：1983-06-01 00:00:00

  • Macrophages are essential for antitumour effects against weakly immunogenic murine tumours induced by class B CpG-oligodeoxynucleotides.

    abstract：:We explored the mechanisms of class B CpG-oligodeoxynucleotide-induced antitumour effects against weakly immunogenic tumours. Treatment with CpG-oligodeoxynucleotide 1826 (CpG) induced similar antitumour effects in B16 melanoma-bearing immunocompetent C57BL/6 mice and T-cell-deficient severe combined immunodeficient (...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2006.02517.x

    authors： Buhtoiarov IN,Sondel PM,Eickhoff JC,Rakhmilevich AL

    更新日期：2007-03-01 00:00:00

  • The macrophage and the apoptotic cell: an innate immune interaction viewed simplistically?

    abstract：:Macrophages play important roles in the clearance of dying and dead cells. Typically, and perhaps simplistically, they are viewed as the professional phagocytes of apoptotic cells. Clearance by macrophages of cells undergoing apoptosis is a non-phlogistic phenomenon which is often associated with actively anti-inflamm...

    journal_title：Immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2567.2004.01959.x

    authors： Gregory CD,Devitt A

    更新日期：2004-09-01 00:00:00

  • Role of myeloid cells in the regulation of group 2 innate lymphoid cell-mediated allergic inflammation.

    abstract：:Group 2 innate lymphoid cells (ILC2s) are an important component of the innate immune system that execute important effector functions at barrier surfaces, such as lung and skin. Like T helper type 2 cells, ILC2s are able to release high amounts of type 2 cytokines that are essential in inducing allergic inflammation ...

    journal_title：Immunology

    pub_type： 杂志文章,评审

    doi：10.1111/imm.13232

    authors： Lei A,He Y,Yang Q,Li X,Li R

    更新日期：2020-09-01 00:00:00

  • S100A8, S100A9 and S100A12 activate airway epithelial cells to produce MUC5AC via extracellular signal-regulated kinase and nuclear factor-κB pathways.

    abstract：:Airway mucus hyperproduction is a common feature of chronic airway diseases such as severe asthma, chronic obstructive pulmonary disease and cystic fibrosis, which are closely associated with neutrophilic airway inflammation. S100A8, S100A9 and S100A12 are highly abundant proteins released by neutrophils and have been...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.12352

    authors： Kang JH,Hwang SM,Chung IY

    更新日期：2015-01-01 00:00:00

  • Antigen-induced non-specific suppressor factor in sheep efferent lymph is prostaglandin E2.

    abstract：:Efferent lymph from the popliteal node of sheep challenged with antigen was found to suppress the in vitro transformation of sheep peripheral blood lymphocytes to a variety of antigens. The suppressor factor appeared 6-20 hr after challenged of the node and was shown to be prostaglandin E2, probably complexed to album...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Hopkins J,McConnell I,Raniwalla J

    更新日期：1981-05-01 00:00:00

  • The potential of CD4 T-cell memory.

    abstract：:While many aspects of memory T-cell immunobiology have been characterized, we suggest that we know only a fraction of the effector functions that CD4 T cells can bring to bear during secondary challenges. Exploring the full impact of memory CD4 T-cell responses is key to the development of improved vaccines against ma...

    journal_title：Immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2567.2010.03259.x

    authors： McKinstry KK,Strutt TM,Swain SL

    更新日期：2010-05-01 00:00:00

  • Suppressor cells induced by BCG release non-specific factors in vitro which inhibit DNA synthesis and interleukin-2 production.

    abstract：:Mice injected intravenously with a high dose (5 X 10(7) ) of BCG fail to develop delayed hypersensitivity to BCG and are described as anergic or unresponsive. Spleen cells from these mice release factors on culture which suppress DNA synthesis induced by concanavalin A in vitro. Cell separation experiments showed that...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Colizzi V,Ferluga J,Garreau F,Malkovsky M,Asherson GL

    更新日期：1984-01-01 00:00:00

  • I. Evidence that lymphotoxin activity involves both cytotoxic and stimulating factors.

    abstract：:This paper describes experiments undertaken to determine more uniform conditions for the generation of cytotoxic lymphokine--'lymphotoxin' (LT) in differently stimulated rat lymphocyte cultures, and to compare the sensitivity of different test systems for detecting rat LT activity in vitro. Wistar rat lymphoid cells w...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ryzewska AG,Darbrowska BK

    更新日期：1976-11-01 00:00:00

  • Functions of interleukin-34 and its emerging association with rheumatoid arthritis.

    abstract：:Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic, synovial inflammation affecting multiple joints, finally leading to extra-articular lesions for which limited effective treatment options are currently available. Interleukin-34 (IL-34), recently discovered as the second colony-stimul...

    journal_title：Immunology

    pub_type： 杂志文章,评审

    doi：10.1111/imm.12660

    authors： Zhou RP,Wu XS,Xie YY,Dai BB,Hu W,Ge JF,Chen FH

    更新日期：2016-12-01 00:00:00

  • The anti-lipid A monoclonal antibody E5 binds to rough gram-negative bacteria, fixes C3, and facilitates binding of bacterial immune complexes to both erythrocytes and monocytes.

    abstract：:Treatment of patients with septic shock using monoclonal antibodies (mAbs) to endotoxin is still controversial. Clinical trials of E5, one of the mAbs directed against the lipid A moiety of lipopolysaccharide (LPS), are currently in progress. The mechanisms of action of this, and other antibodies under clinical evalua...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Seelen MA,Athanassiou P,Lynn WA,Norsworthy P,Walport MJ,Cohen J,Davies KA

    更新日期：1995-04-01 00:00:00

  • Relative immunogenicity of hepatitis B virus-encoded antigens as targets for cytotoxic T-cell response.

    abstract：:To analyse the immunological mechanism of hepatocellular injury in hepatitis B virus (HBV) infection, the immunoreactivity of HBV-encoded antigens as a target for cytotoxic T lymphocyte (CTL) response was examined using recombinant vaccinia virus (RVV) expressing surface protein (S), precore/core protein (PC), and cor...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ishikawa T,Kakumu S,Yoshioka K,Yamada M,Tanaka K,Higashi Y,Takayanagi M,Okumura A,Kojima A,Tamura T

    更新日期：1993-10-01 00:00:00

  • Analysis of immunoglobulin E VH transcripts in a bronchial biopsy of an asthmatic patient confirms bias towards VH5, and indicates local clonal expansion, somatic mutation and isotype switch events.

    abstract：:Immunoglobulin E (IgE)-dependent mechanisms play a pivotal role in mediating allergic disease. Previously, VH-Cepsilon transcripts from blood or spleen of atopic asthmatics have been analysed for VH gene usage and patterns of somatic mutation. An over-representation of the minor VH5 family has been observed, consisten...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1999.00910.x

    authors： Snow RE,Djukanovic R,Stevenson FK

    更新日期：1999-12-01 00:00:00

  • Anti-anti-idiotypic (Ab3) antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from antibodies raised directly against the antigen.

    abstract：:Polyclonal rabbit anti-idiotypic (Ab2) antibodies raised against the antiprogesterone mAb DB3 (Ab1) were used to induce an Ab3 antiprogesterone response in BALB/c mice. While the affinity of Ab3 sera for progesterone was 10-50-times lower than that of DB3, their steroid-binding specificity showed considerable similari...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2000.00006.x

    authors： Kirsch RD,Beale D,He M,Corper AL,Krawinkel-Brenig U,Taussig MJ

    更新日期：2000-06-01 00:00:00

  • Putative role for interleukin-7 in the maintenance of the recirculating naive CD4+ T-cell pool.

    abstract：:The capacity of the immune system to respond efficiently to new antigens depends upon a continuous source of naive CD4+ T cells. Such cells exit from the thymus and join the recirculated T-cell pool. Factors present at the sites of naive CD4+ T-cell circulation must be responsible for their survival, since upon remova...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1999.00906.x

    authors： Webb LM,Foxwell BM,Feldmann M

    更新日期：1999-11-01 00:00:00

  • Appearance of the LAT protein at an early stage of B-cell development and its possible role.

    abstract：:The linker protein LAT is expressed mainly in T and natural killer (NK) cells. LAT-deficient mice have an arrest of intrathymic T-cell development at the CD4+ CD8+ stage and lack mature T cells in the periphery. However, no gross abnormality in development and function of the B and NK cells has been described. Here we...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2003.01671.x

    authors： Oya K,Wang J,Watanabe Y,Koga R,Watanabe T

    更新日期：2003-07-01 00:00:00

  • Suppress of the increase in free cytosolic calcium during the inhibition of T-cell activation by an autoantibody present in the serum of leprosy patients.

    abstract：:A serum factor, believed to be an IgG autoantibody, in certain patients with lepromatous leprosy inhibits the proliferation of mitogen-stimulated lymphocytes. To investigate which stage of the cell cycle was inhibited, we examined the effect of these sera on the kinetics of lymphocyte activation induced by several mit...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Poulton TA,Gallagher A,Beck JS

    更新日期：1989-11-01 00:00:00

  • The differential effect of cyclic AMP on lymphocyte stimulation by T- or B-cell mitogens.

    abstract：:Dibutyryl-cyclic adenosine 3'5'-monophosphate (cAMP) added to mouse spleen cell cultures under serum free conditions inhibited the stimulation of [3H]thymidine incorporation induced by T-cell mitogens much more than the one induced by B-cell mitogens. The inhibition was most impressive with phytohaemagglutinin, follow...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Vischer TL

    更新日期：1976-05-01 00:00:00

  • Interleukin-32 enhances cytotoxic effect of natural killer cells to cancer cells via activation of death receptor 3.

    abstract：:Studies have demonstrated that the anti-tumour effect of natural killer (NK) cells is successful for patients with several cancers. Although interleukin-32 (IL-32) is endogenously expressed in NK cells, cytolytic function of NK cells against cancer cells has not been fully demonstrated. In the present study, we found ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2011.03513.x

    authors： Park MH,Song MJ,Cho MC,Moon DC,Yoon DY,Han SB,Hong JT

    更新日期：2012-01-01 00:00:00

  • Genomic expression analysis by single-cell mRNA differential display of quiescent CD8 T cells from tumour-infiltrating lymphocytes obtained from in vivo liver tumours.

    abstract：:We performed a genomic study combining single-cell mRNA differential display and RNA subtractive hybridization to elucidate CD8 T-cell quiescence/ignorance. By comparing actively maintained quiescent CD8 T cells from liver tumour tumour-infiltrating lymphocytes (TILs) with quiescent T cells at the single-cell level, w...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2008.02926.x

    authors： Zhang W,Ding J,Qu Y,Hu H,Lin M,Datta A,Larson A,Liu GE,Li B

    更新日期：2009-05-01 00:00:00

  • Oestrogen up-regulates interleukin-21 production by CD4(+) T lymphocytes in patients with systemic lupus erythematosus.

    abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease in which abnormal immune responses are mediated by tissue-binding autoantibodies and immune complex deposition. Because most SLE patients are women of child-bearing age, oestrogen has been suggested to play an important role in SLE pathogenesis. One proposed ...

    journal_title：Immunology

    pub_type： 临床试验,杂志文章

    doi：10.1111/imm.12263

    authors： Lee J,Shin EK,Lee SY,Her YM,Park MK,Kwok SK,Ju JH,Park KS,Kim HY,Cho ML,Park SH

    更新日期：2014-08-01 00:00:00

  • Treatment with recombinant granulocyte colony-stimulating factor (Filgrastin) stimulates neutrophils and tissue macrophages and induces an effective non-specific response against Mycobacterium avium in mice.

    abstract：:A role of neutrophils in the host response against Mycobacterium avium (MAC) has recently been suggested. To investigate this matter further, we determined the effect of granulocyte colony-stimulating factor (G-CSF) on the outcome of MAC infection in mice. C57BL/6bg+/bg- black mice were intravenously infected with 1 x...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1998.00529.x

    authors： Bermudez LE,Petrofsky M,Stevens P

    更新日期：1998-07-01 00:00:00

  • A novel Zap70 mutation with reduced protein stability demonstrates the rate-limiting threshold for Zap70 in T-cell receptor signalling.

    abstract：:Loss of ζ-associated protein 70 (Zap70) results in severe immunodeficiency in humans and mice because of the critical role of Zap70 in T-cell receptor (TCR) signalling. Here we describe a novel mouse strain generated by N-ethyl-N-nitrosourea mutagenesis, with the reduced protein stability (rps) mutation in Zap70. The ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.12199

    authors： Cauwe B,Tian L,Franckaert D,Pierson W,Staats KA,Schlenner SM,Liston A

    更新日期：2014-03-01 00:00:00

  • The effect of corticosteroids upon murine B cells in vivo and in vitro as determined in the LPS-culture system.

    abstract：:The influence of the synthetic corticosteroid dexamethasone sodium phosphate (DEXA) upon mouse B cells was studied. This was done by in vivo treatment of mice with a single or multiple injection of DEXA, and by culturing murine spleen cells and bone marrow cells in vitro in the presence of different concentrations of ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Sabbele NR,Van Oudenaren A,Hooijkaas H,Benner R

    更新日期：1987-10-01 00:00:00

  • Development of an epitope panel for consistent identification of antigen-specific T-cells in humans.

    abstract：:We aimed to establish a panel of MHC-peptide multimers suitable as a positive control in the detection of HLA A*0201 restricted antigen specific T cells (ASTC) by flow cytometry. MHC Dextramers were loaded with HLA A*0201 binding peptides from viral antigens and melanoma targets identified from a literature search and...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.12769

    authors： Fløe A,Løppke C,Hilberg O,Wejse C,Brix L,Jacobsen K

    更新日期：2017-10-01 00:00:00