Abstract:
:The outer membrane proteins of Bordetella avium were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sarkosyl-insoluble outer membrane protein-enriched profiles from 50 virulent B. avium isolates, containing major 21,000- and 37,000-molecular-weight proteins (21K and 37K proteins, respectively) and at least 13 less intensely stained proteins with molecular weights ranging from 13,500 to 143,000, were very similar. The 21K, 27K, 31K, and 37K outer membrane proteins were shown to be associated noncovalently with the underlying peptidoglycan layer. It was necessary to treat cell envelopes with 2% sodium dodecyl sulfate and at temperatures in excess of 60 degrees C for 15 min to release these proteins. Exposure of proteins on the cell surface of B. avium was assessed by labeling with 125I followed by electrophoresis. As many as 13 bands were present in profiles from labeled whole cells. Of the surface-labeled bands, eight corresponded to bands in a radiolabeled outer membrane preparation. The outer membrane protein profile of B. avium was compared with profiles from other Bordetella spp., including 20 B. avium-like and 16 B. bronchiseptica strains isolated from turkeys. The outer membrane protein profile of B. avium was distinctly different from those of the other bordetella. The effect of variations in the growth medium on the expression of outer membrane proteins of B. avium was examined. Expression of 22K, 26K, 56K, and 73K proteins was decreased or eliminated by addition of 50 mM MgSO4 to the medium.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Leyh R,Griffith RWdoi
10.1128/IAI.60.3.958-964.1992keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
958-64issue
3eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::The ability to bind Congo red (Crb+) is associated with virulence of Shigella flexneri and is encoded by a large, 220-kilobase plasmid. We cloned fragments of this plasmid to isolate the sequences encoding Congo red binding, to determine the degree of conservation of these sequences among S. flexneri strains, and to s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.435-443.1986
更新日期:1986-11-01 00:00:00
abstract::Resequencing estA3, an allele of the methanol-soluble heat-stable enterotoxin of Escherichia coli showed that the proline triplet 19 is in fact an alanine codon; thus, estA alleles 3 and 4 were shown to be identical. Resequencing has also shown that the carboxy terminus of another allele, estA2, is not the previously ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.645-648.1989
更新日期:1989-02-01 00:00:00
abstract::Many of the proinflammatory effects of gram-negative bacteria are elicited by the interaction of bacterial lipopolysaccharide (LPS) with Toll-like receptor 4 (TLR4) expressed on host cells. TLR4 signaling leads to activation of NF-kappa B and transcription of many genes involved in the inflammatory response. In this s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4414-4420.2003
更新日期:2003-08-01 00:00:00
abstract::The Salmonella typhimurium gene for Mn-cofactored superoxide dismutase (sodA) was cloned by complementation of an Escherichia coli sodA sodB mutant for growth on minimal medium. Sequence analysis revealed an open reading frame of 618 bp encoding a polypeptide with 97% identity to E. coli SodA. A S. typhimurium sodA mu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1739-1744.1995
更新日期:1995-05-01 00:00:00
abstract::Intact tissues are relatively resistant to Pseudomonas aeruginosa-induced disease, and injury predisposes tissue to infection. Intact epithelia contain polarized cells that have distinct apical and basolateral membranes with unique lipids and proteins. In this study, the role of cell polarity in epithelial cell suscep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2861-2867.1997
更新日期:1997-07-01 00:00:00
abstract::Active immunization with Escherichia coli-expressed recombinant outer surface protein C (OspC) of Borrelia burgdorferi has been demonstrated to confer protection against a tick-transmitted infection on laboratory animals. A previous study in this laboratory showed that OspC antibody raised against a denatured immunoge...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5463-5469.1999
更新日期:1999-10-01 00:00:00
abstract::This work evaluated the kinetics of events that occur in the placenta of Calomys callosus after Toxoplasma gondii infection. Animals on the first day of pregnancy (dop) and virgin nonpregnant females were perorally infected with 20 cysts of T. gondii strain ME49. After 100 days of infection, the virgin animals were ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.7089-7094.2002
更新日期:2002-12-01 00:00:00
abstract::We studied apoptosis in a human ileocecal adenocarcinoma tumor cell line (HCT-8) infected with Cryptosporidium parvum, from 2 to 72 h postinfection (h.p.i.). At 2 h.p.i., the percentage of annexin V-positive cells in the cell culture had increased to 10% compared to 2.5% in noninfected control culture; sorted infected...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.6061-6067.2004
更新日期:2004-10-01 00:00:00
abstract::Tox-+ staphylococcal strains, as opposed to Tox-minus strains, produce epidermal exfoliation within 18 h after direct subcutaneous or intraperitoneal injection into newborn mice. The extracellular product responsible for exfoliation is termed exfoliative toxin (ET). When culture supernatant fluid from the plasmid-cure...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.1.193-197.1975
更新日期:1975-07-01 00:00:00
abstract::The enterotoxin of Shigella dysenteriae I produces fluid and electrolyte secretion in the rabbit ileum. These present studies were designed to evaluate nonelectrolyte transport in rabbit ileal mucosa exposed to Shigella enterotoxin. Both 10 mM galactose and 5 mM L-alanine absorptions were significantly impaired in ent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.510-512.1977
更新日期:1977-05-01 00:00:00
abstract::Oral administration of polymyxin to specific-pathogen-free C3H/Law mice which with previously contaminated with gram-negative bacteria resulted in complete suppression of cecal gram-negative bacteria. Suppression of cecal gram-negative bacteria was accompanied by reduction of the cecal endotoxin concentration from 10 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.437-441.1985
更新日期:1985-11-01 00:00:00
abstract::The hydrolysis of urea by ureases of oral bacteria in dental plaque can cause a considerable increase in plaque pH, which can inhibit the development of dental caries. There is also indirect evidence that urea metabolism may promote the formation of calculus and that ammonia release from urea could exacerbate periodon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6670-6676.2000
更新日期:2000-12-01 00:00:00
abstract::Recent preclinical and epidemiologic studies have suggested that certain Mycobacterium tuberculosis genotypes (in particular, Beijing lineage strains) may be resistant to Mycobacterium bovis BCG vaccine-induced antituberculosis protective immunity. To investigate the strain specificity of BCG-induced protective respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00019-08
更新日期:2008-11-01 00:00:00
abstract::The cyst cell wall β-glucans of Pneumocystis have been shown to stimulate immune responses in lung epithelial cells, dendritic cells, and alveolar macrophages. Little is known about how the trophic life forms, which do not have a fungal cell wall, interact with these innate immune cells. Here, we report differences in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00519-16
更新日期:2016-11-01 00:00:00
abstract::No effective therapy exists for Cryptosporidium parvum, a coccidial protozoan parasite that causes severe diarrhea in patients with AIDS. The role of microtubules in parasite invasion of host cells was investigated by incubating 10(7) oocysts with a HT 29.74 cell line for 24 h in the presence of microtubule-disrupting...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4888-4890.1993
更新日期:1993-11-01 00:00:00
abstract::Passive protection, the administration of antibodies to prevent infection, has garnered significant interest in recent years as a potential prophylactic countermeasure to decrease the prevalence of hospital-acquired infections. Pili, polymerized protein structures covalently anchored to the peptidoglycan wall of many ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01403-13
更新日期:2014-04-01 00:00:00
abstract::Results from studies using mice deficient in specific complement factors and clinical data on patients with an inherited deficiency of the classical complement pathway component C2 suggest that the classical pathway is vital for immunity to Streptococcus pneumoniae. However, the consequences of defects in classical pa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-08
更新日期:2008-08-01 00:00:00
abstract::Experiments outlined here investigate the role of nitric oxide (NO) in the pathogenesis of Plasmodium falciparum-induced malarial anemia (MA). The results show that ex vivo and in vitro NO synthase (NOS) activity in peripheral blood mononuclear cells (PBMCs) is significantly elevated in children with MA and inversely ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4868-4873.2004
更新日期:2004-08-01 00:00:00
abstract::CD8+ T cells are essential for protection against mycobacteria, as is clearly demonstrated by the fatal outcome of experimental infection of beta-2 microglobulin knockout mice. However, the mechanisms and antigens (Ags) leading to CD8+ T-cell activation and regulation have been poorly characterized. Here we show that,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.676-684.1997
更新日期:1997-02-01 00:00:00
abstract::Several trivalent cations, including lanthanum (La3+), inhibited the secretion (enterosorption) induced by the enterotoxins of Vibrio cholerae and Escherichia coli in the rabbit ileum in vivo. High concentrations (greater than 10 mM) of La3+ were required to inhibit cholera enterotoxin (CE)-induced enterosorption, pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1038-1044.1975
更新日期:1975-05-01 00:00:00
abstract::Bacillary angiomatosis (BA), one of the many clinical manifestations resulting from infection with the facultative intracellular bacterium Bartonella henselae, is characterized by angiogenic lesions. Macrophages have been identified as important effector cells contributing to the angiogenic process during B. henselae ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5735-5742.2005
更新日期:2005-09-01 00:00:00
abstract::Representative strains of serogroup A Neisseria meningitidis were chosen from all major meningitis epidemics worldwide since 1960 and subjected to analysis for the electrophoretic variation of 15 cytoplasmic allozymes and four outer membrane proteins. The 290 strains defined 84 unique electrophoretic types which were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5267-5282.1992
更新日期:1992-12-01 00:00:00
abstract::The ability of hemagglutinating and poorly hemagglutinating strains of the gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled laminin was quantitated in a liquid phase assay. Although all strains bound laminin, some hemagglutinating strains were good binders of laminin (maximum of 31% binding), whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3640-3648.1994
更新日期:1994-09-01 00:00:00
abstract::Bacillus anthracis, the causative agent of anthrax, relies on multiple virulence factors to subvert the host immune defense. Using Caenorhabditis elegans as an infection model, we screened approximately 5,000 transposon mutants of B. anthracis Sterne for decreased virulence. One of the attenuated mutants resulted in l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01614-13
更新日期:2014-03-01 00:00:00
abstract::The addition of a sonic extract of Capnocytophaga sputigena to the culture fluid to human fibroblasts resulted in an inhibition of cell proliferation. The inhibition was dose-related (200 micrograms/ml caused a 90% inhibition, and 1,000 micrograms/ml caused a complete cessation of growth). The growth inhibition was no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.271-275.1980
更新日期:1980-01-01 00:00:00
abstract::P6, a 16,600-dalton protein present in the outer membranes of both typeable and nontypeable strains, may be an important antigen in immunity to Haemophilus influenzae. The gene encoding P6 of a nontypeable strain of H. influenzae was cloned by using bacteriophage lambda gt11. Four recombinant phages were detected by s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.128-134.1988
更新日期:1988-01-01 00:00:00
abstract::Citrate is a ubiquitous compound and can be utilized by many bacterial species, including enteric pathogens, as a carbon and energy source. Genes involved in citrate utilization have been extensively studied in some enteric bacteria, such as Klebsiella pneumoniae; however, their role in pathogenesis is still not clear...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00746-18
更新日期:2019-02-21 00:00:00
abstract::Diarrhea and amebic liver abscesses due to invasive Entamoeba histolytica infections are an important cause of morbidity and mortality in the developing world. Entamoeba histolytica adherence and cell migration, two phenotypes linked to virulence, are both aberrant in trophozoites deficient in the metallosurface prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02490-14
更新日期:2015-02-01 00:00:00
abstract::For pathogens to survive in the human oral cavity, they must identify a suitable niche in the complex multispecies biofilm that exists on oral tissues. The periodontal pathogen Porphyromonas gingivalis adheres to Streptococcus gordonii by interacting with a specific region of the streptococcal SspB polypeptide, design...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5736-5741.2001
更新日期:2001-09-01 00:00:00
abstract::The effects of 1,25-dihydroxy vitamin D3 and gamma interferon on the phenotypic changes associated with monocyte maturation in vitro were investigated. Human monocytes separated from peripheral blood mononuclear cell populations by adherence to plastic were cultured for 7 days on glass. Immunocytological analysis was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2017-2020.1987
更新日期:1987-09-01 00:00:00