Abstract:
:Programmed cell death (PCD) is regulated by multiple evolutionarily conserved mechanisms to ensure the survival of the cell. Here we describe pvl-5, a gene that likely regulates PCD in Caenorhabditis elegans. In wild-type hermaphrodites at the L2 stage there are 11 Pn.p hypodermal cells in the ventral midline arrayed along the anterior-posterior axis and 6 of these cells become the vulval precursor cells. In pvl-5(ga87) animals there are fewer Pn.p cells (average of 7.0) present at this time. Lineage analysis reveals that the missing Pn.p cells die around the time of the L1 molt in a manner that often resembles the programmed cell deaths that occur normally in C. elegans development. This Pn.p cell death is suppressed by mutations in the caspase gene ced-3 and in the bcl-2 homolog ced-9, suggesting that the Pn.p cells are dying by PCD in pvl-5 mutants. Surprisingly, the Pn.p cell death is not suppressed by loss of ced-4 function. ced-4 (Apaf-1) is required for all previously known apoptotic cell deaths in C. elegans. This suggests that loss of pvl-5 function leads to the activation of a ced-3-dependent, ced-4-independent form of PCD and that pvl-5 may normally function to protect cells from inappropriate activation of the apoptotic pathway.
journal_name
Geneticsjournal_title
Geneticsauthors
Joshi P,Eisenmann DMdoi
10.1534/genetics.103.020503keywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
673-85issue
2eissn
0016-6731issn
1943-2631pii
167/2/673journal_volume
167pub_type
杂志文章相关文献
GENETICS文献大全abstract::The process of close recombinant formation in bacteriophage T5 crosses has been studied by examining the structure of internal heterozygotes (HETs), the immediate products of recombination events. The T5 system was chosen because it permits the study of internal heterozygotes exclusively, thus avoiding the ambiguities...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::A major correlation has been found between the incidence of glucocorticoid-induced cleft palate and the chromosome 8 segment identified by N-acetyl transferase in mice. The resistant strain became fully susceptible while the susceptible strain became resistant when this chromosomal region, representing less than 0.7% ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::The ability of autonomously replicating plasmids to recombine in mammalian cells was investigated. Two deletion plasmids of the eukaryotic-prokaryotic shuttle vector pSV2neo were cotransfected into transformed monkey COS cells. Examination of the low molecular weight DNA isolated after 48 hr of incubation revealed tha...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::Mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase, but, unlike nuc-1 and nuc-2 mutants, are able to make the repressible acid phosphatase and the repressible phosphate permease under conditions of derepression (phosphate deprivation). The new mutants, called pho-2, map in L...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-10-01 00:00:00
abstract::Comparison of population differentiation in neutral marker genes and in genes coding quantitative traits by means of F(ST) and Q(ST) indexes has become commonplace practice. While the properties and estimation of F(ST) have been the subject of much interest, little is known about the precision and possible bias in Q(S...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.044545
更新日期:2005-11-01 00:00:00
abstract::Adaptation from de novo mutation can produce so-called soft selective sweeps, where adaptive alleles of independent mutational origin sweep through the population at the same time. Population genetic theory predicts that such soft sweeps should be likely if the product of the population size and the mutation rate towa...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.165571
更新日期:2014-10-01 00:00:00
abstract::The parasitoid wasp Nasonia vitripennis is an emerging model organism for developmental and behavioral genetics. It reproduces by haplodiploidy; males typically develop parthenogenetically from haploid eggs and females from fertilized diploid eggs. A polyploid mutant strain is available in which females are triploid a...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.044743
更新日期:2006-02-01 00:00:00
abstract::Key steps of essential metabolic pathways are housed in plant peroxisomes. We conducted a microscopy-based screen for anomalous distribution of peroxisomally targeted fluorescence in Arabidopsis thaliana This screen uncovered 34 novel alleles in 15 genes affecting oil body mobilization, fatty acid β-oxidation, the gly...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.193169
更新日期:2016-11-01 00:00:00
abstract::The effective population size (N(e)) is notoriously difficult to accurately estimate in wild populations as it is influenced by a number of parameters that are difficult to delineate in natural systems. The different methods that are used to estimate N(e) are affected variously by different processes at the population...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.136580
更新日期:2012-06-01 00:00:00
abstract::Variations in K(+) channel composition allow for differences in cell excitability and, at an organismal level, provide flexibility to behavioral regulation. When the function of a K(+) channel is disrupted, the remaining K(+) channels might incompletely compensate, manifesting as abnormal organismal behavior. In this ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.137455
更新日期:2012-03-01 00:00:00
abstract::We have examined the effects of prolonged histidine deprivation on the reversion of Salmonella typhimurium histidine auxotrophs containing either hisG46, a missense mutation (CTC----CCC), or hisG428, an ochre mutation (CAA----TAA). Both of these mutants can revert to His+ via intragenic and extragenic mechanisms. Wher...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Most studies of mutation rates implicitly assume that they remain constant throughout development of the germline. However, researchers recently used a novel statistical framework to reveal that mutation rates differ dramatically during sperm development in Drosophila melanogaster. Here a general framework is describe...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.151571
更新日期:2013-08-01 00:00:00
abstract::Centromeres are responsible for the correct segregation of chromosomes during mitosis and meiosis. Holocentric chromosomes, characterized by multiple centromere units along each chromatid, have particular adaptations to ensure regular disjunction during meiosis. Here we show by detecting CENH3, CENP-C, tubulin, and ce...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.191213
更新日期:2016-10-01 00:00:00
abstract::The fine-scale pattern of correlated paternity was characterized within a population of the narrow-endemic model plant species, Centaurea corymbosa, using microsatellites and natural progeny arrays. We used classical approaches to assess correlated mating within sibships and developed a new method based on pairwise ki...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.027714
更新日期:2004-11-01 00:00:00
abstract::Pathogen resistance is an ecologically important phenotype increasingly well understood at the molecular genetic level. In this article, we examine levels of avrRpt2-dependent resistance and Rps2 locus DNA sequence variability in a worldwide sample of 27 accessions of Arabidopsis thaliana. The rooted parsimony tree of...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-02-01 00:00:00
abstract::Gene targeting techniques have led to the phenotypic characterization of numerous genes; however, many genes show minimal to no phenotypic consequences when disrupted, despite many having highly conserved sequences. The standard explanation for these findings is functional redundancy. A competing hypothesis is that th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.178079
更新日期:2015-10-01 00:00:00
abstract::Gametes are produced in plants through mitotic divisions in the haploid gametophytes. We investigated the role of EXPORTIN1 (XPO1) genes during the development of both female and male gametophytes of Arabidopsis. Exportins exclude target proteins from the nucleus and are also part of a complex recruited at the kinetoc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.094896
更新日期:2008-11-01 00:00:00
abstract::An exact sampling formula for a Wright-Fisher population of fixed size N under the infinitely many neutral alleles model is deduced. This extends the Ewens formula for the configuration of a random sample to the case where the sample is drawn from a population of small size, that is, without the usual large-N and smal...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.077644
更新日期:2007-10-01 00:00:00
abstract::We previously identified a transcriptional regulatory element, which we call NRE(DIT), that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae. Repression through this element is dependent on the Ssn6-Tup1 corepressor. In this study, we sho...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::We subjected the genes encoding the 19.3-, 21.3c-, and 51-kDa iron-sulfur subunits of respiratory chain complex I from Neurospora crassa to site-directed mutagenesis to mimic mutations in human complex I subunits associated with mitochondrial diseases. The V135M substitution was introduced into the 19.3-kDa cDNA, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.041517
更新日期:2005-09-01 00:00:00
abstract::This work demonstrates that environmental conditions experienced by individuals can shape their development and affect the stability of genetic associations. The implication of this observation is that the environmental response may influence the evolution of traits in the wild. Here, we examined how the genetic archi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.086801
更新日期:2009-04-01 00:00:00
abstract::The fission yeast DNA repair gene rad21+ is essential for cell growth. To investigate the function essential for cell proliferation, we have isolated a temperature-sensitive mutant of the rad21+ gene. The mutant, rad21-K1, showed abnormal mitosis at the nonpermissive temperature. Some cells contained abnormal nuclear ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The paralogous maize proteins EMPTY PERICARP2 (EMP2) and HEAT SHOCK FACTOR BINDING PROTEIN2 (HSBP2) each contain a single recognizable motif: the coiled-coil domain. EMP2 and HSBP2 accumulate differentially during maize development and heat stress. Previous analyses revealed that EMP2 is required for regulation of hea...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026575
更新日期:2004-07-01 00:00:00
abstract::In the sensory system of C. elegans, the candidate odorant receptor gene str-2 is strongly expressed in one of the two AWC neurons and weakly in both ASI neurons. Asymmetric AWC expression results from suppression of str-2 expression by a Ca2+/MAPK signaling pathway in one of the AWC neurons early in development. Here...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.058750
更新日期:2006-07-01 00:00:00
abstract::Amoebae of the Colonia (CL) strain, which normally produce plasmodia in clones, are shown to cross with mt3 and mt4 amoebae under appropriate conditions. Strains carrying mt3 and mt4 in a CL genetic background were constructed and used in the genetic analysis of mutants isolated in CL. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-12-01 00:00:00
abstract::Many genetic variants identified in genome-wide association studies (GWAS) are associated with multiple, sometimes seemingly unrelated, traits. This motivates multi-trait association analyses, which have successfully identified novel associated loci for many complex diseases. While appealing, most existing methods foc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303242
更新日期:2020-08-01 00:00:00
abstract::Several human pathogens (e.g., Bacillus anthracis, Yersinia pestis, Bordetella pertussis, Plasmodium falciparum, and Mycobacterium tuberculosis) have very restricted unselected allelic variation in structural genes, which hinders study of the genetic relationships among strains and strain-trait correlations. To addres...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Mitomycin C (MMC) is a genotoxic agent that induces DNA cross-links, DNA alkylation, and the production of reactive oxygen species (ROS). MMC induces the SOS response and RpoS regulons in Escherichia coli SOS-encoded functions are required for DNA repair, whereas the RpoS regulon is typically induced by metabolic stre...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.199471
更新日期:2017-07-01 00:00:00
abstract::The breeding systems of many organisms are cryptic and difficult to investigate with observational data, yet they have profound effects on a species' ecology, evolution, and genome organization. Genomic approaches offer a novel, indirect way to investigate breeding systems, specifically by studying the transmission of...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.179879
更新日期:2015-11-01 00:00:00
abstract::The DC2 gene was isolated previously on the basis of sequence similarity to DC0, the major Drosophila protein kinase A (PKA) catalytic subunit gene. We show here that the 67-kD Drosophila DC2 protein behaves as a PKA catalytic subunit in vitro. DC2 is transcribed in mesodermal anlagen of early embryos. This expression...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00