Abstract:
:We present a method that permits the simultaneous analysis of carbamazepine (CBZ) and its major biotransformation products, carbamazepine-10,11-epoxide (CBZ-E) and carbamazepine-10,11-dihydroxide (CBZ-diOH), in plasma samples. The method consists of plasma extraction in alkaline medium with NaCl added using chloroform-ethyl acetate (1:1, v/v) and later purification with n-hexane. The samples were submitted to reversed-phase chromatography (RP-18) using acetonitrile-water (3:7, v/v) as the mobile phase and detection at 220 nm. Recoveries of 62.0, 99.9, and 105.4% were obtained for CBZ-diOH, CBZ-E, and CBZ, respectively, with sensitivities of 0.32 micrograms/mL for CBZ-E and CBZ-dOH and of 0.64 micrograms/mL for CBZ. The method proved to be specific, thus permitting measurements in situations of drug combinations.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Bonato PS,Lanchote VL,de Carvalho D,Achê Pdoi
10.1093/jat/16.2.88keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
88-92issue
2eissn
0146-4760issn
1945-2403journal_volume
16pub_type
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