Abstract:
OBJECTIVE:To investigate the feasibility of using a ganglial culture system to screen various growth factors as potential therapeutic agents for pelvic nerve injuries. MATERIALS AND METHODS:The major pelvic ganglia (MPG) were isolated from male rats and attached to culture dishes with the aid of Matrigel (Becton Dickinson, Mountain View, CA, USA). Alternatively, the dorso-caudal region (DCR) of MPG, from which the cavernous nerves originate, was dissected and then attached to a Matrigel-coated coverslip. The MPG or DCR was cultured in serum-free medium supplemented with phosphate-buffered saline (PBS, control), 50 ng/mL of vascular endothelial growth factor (VEGF), 20 ng/mL of a neurotrophin (BDNF, NT3, or NT4), or combinations of these growth factors. After 2 days of incubation, the ganglial tissues with their outgrowing nerve fibres were stained for the expression of NADPH-diaphorase, tyrosine hydroxylase (TH) and acetylcholinesterase (AChE). The length and staining intensity of nerve fibres were analysed. RESULTS:The outgrowing fibres were significantly longer in MPG treated with any of the four tested growth factors than in PBS-treated MPG. The combination of VEGF and NT3 induced the best fibre growth. Improvements to the culturing conditions allowed a histological examination of the outgrowing fibres for the expression of nitric oxide synthase (NOS), TH and AChE. VEGF and BDNF were equally capable of inducing NOS- and TH-expressing fibres. BDNF was much weaker than VEGF for inducing AChE-expressing fibres. CONCLUSIONS:This improved culturing system is potentially useful for screening nerve-regenerating factors; VEGF had neurotrophic effects comparable with BDNF, NT3, or NT4.
journal_name
BJU Intjournal_title
BJU internationalauthors
Lin G,Chen KC,Hsieh PS,Yeh CH,Lue TF,Lin CSdoi
10.1046/j.1464-410x.2003.04439.xkeywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
631-5issue
6eissn
1464-4096issn
1464-410Xpii
4439journal_volume
92pub_type
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05828.x
更新日期:2005-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/bju.12000
更新日期:2013-05-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2001.02375.x
更新日期:2001-10-01 00:00:00
abstract:UNLABELLED:WHAT'S KNOWN ON THE SUBJECT? AND WHAT DOES THE STUDY ADD?: In renal cell carcinoma the role of lymphadenectomy (LND) is still controversial. Moreover, no firm consensus exists regarding the minimum number of lymph nodes that should be removed to obtain a satisfactory staging LND at the time of surgery. Our f...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2012.11313.x
更新日期:2013-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1464-410x.2001.00100.x
更新日期:2001-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1464-410x.2002.03027.x
更新日期:2002-12-01 00:00:00
abstract:STUDY TYPE:Therapy (case series) Level of Evidence 4. OBJECTIVE:To report our analysis of the oncological outcome, side-effects and complications after (125)I-brachytherapy, based on 10 years of experience, as low dose-rate (LDR) prostate brachytherapy is an accepted, effective and safe therapy for localized prostate ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2009.09096.x
更新日期:2010-07-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2009.08547.x
更新日期:2009-11-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2010.09273.x
更新日期:2010-10-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2011.10434.x
更新日期:2012-04-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1111/bju.12558
更新日期:2014-09-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.05956.x
更新日期:2006-02-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2007.07337.x
更新日期:2008-04-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06274.x
更新日期:2006-08-01 00:00:00
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更新日期:2019-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:BJU international
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更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2011.010419.x
更新日期:2012-03-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2012.11202.x
更新日期:2012-12-01 00:00:00
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更新日期:2019-08-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1046/j.1464-410x.2001.02033.x
更新日期:2001-02-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.14182
更新日期:2018-11-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2010.09636.x
更新日期:2011-05-01 00:00:00
abstract:UNLABELLED:WHAT'S KNOWN ON THE SUBJECT? AND WHAT DOES THE STUDY ADD?: Prostate cancer has three known clinical risk factors: age, ethnicity and family history. Men's knowledge of prostate cancer is low. This study demonstrates that men rely on family and friends to learn about prostate cancer and help them interpret th...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2012.11316.x
更新日期:2013-03-01 00:00:00
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更新日期:2019-04-01 00:00:00
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更新日期:2008-09-01 00:00:00