Enlargement of calcium oxalate stones to clinically significant size in an in-vitro stone generator.

Abstract:

OBJECTIVE:To develop and validate an in vitro method suitable for the quantitative investigation of the growth of calcium oxalate stones through to a clinically significant size. MATERIALS AND METHODS:Small fragments of calcium oxalate calculi were suspended in a mixed suspension/mixed product removal crystalliser supplied with artificial urine supersaturated with calcium oxalate. The fragments were weighed at regular intervals until they reached approximately equal 500 mg. The results were plotted as weight against time and fitted to equations corresponding to constant increase in diameter, surface area-controlled and constant-deposition growth patterns. The choice of the most appropriate model was based on the squared regression coefficient (r2). RESULTS:Eight fragments (2-6 mm in diameter) were grown to approximately 10 mm in diameter over periods from 137 to 369 h. Seven of the growth curves were best-fitted (r2 > or = 0.988) by the equation w = kt(3/2) + c, where w is the weight, k is a growth constant, t is the time and c is a constant approximating to the initial weight. This corresponds to a surface area-dependent mechanism. CONCLUSIONS:The growth of these small fragments to a clinically significant size accelerated throughout the experimental period in a way which was consistent with a surface area-dependent mechanism. We have developed a resilient model suitable for studying the kinetics of calcium oxalate stone growth in vitro.

journal_name

BJU Int

journal_title

BJU international

authors

Ananth K,Kavanagh JP,Walton RC,Rao PN

doi

10.1046/j.1464-410x.2002.03027.x

keywords:

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

939-44

issue

9

eissn

1464-4096

issn

1464-410X

pii

03027

journal_volume

90

pub_type

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