Abstract:
OBJECTIVE:To examine differences in gene expression levels between renal cell carcinoma (RCC) tissue and 'normal' appearing renal tissue using a commercially available DNA macroarray. MATERIALS AND METHODS:Tissue was obtained from 47 consecutive radical nephrectomies, 29 of which were eligible. DNA macroarrays were analysed on the tumour and normal-appearing control tissue to measure the expression of 1185 cancer-related genes. The group of samples was also stratified according to the presence or absence of granular cells and according to tumour grade. Quantitative real-time polymerase-chain reaction (PCR) was also performed on seven key genes present on the macroarray. RESULTS:In all, 444 genes were over-expressed and 33 genes were under-expressed. Using selection criteria reduced the list to nine that were significantly over-expressed and 23 that were under-expressed. These significant genes belonged to the families of oncogenes, growth factors, interleukins, receptors, immune system components, cytoskeleton, matrix proteins and intracellular modulators, or they coded for proteins involved in DNA transcription and RNA translation, DNA repair, protein turnover, and metabolism of carbohydrates and lipids. There were differences in gene expression according to the presence or absence of granular cells and according to tumour grade. Using quantitative real-time PCR there was over-expression of epidermal growth factor receptor, c-myc, transforming growth factor-alpha, vascular endothelial growth factor and vimentin, and under-expression of TYRO3 protein tyrosine kinase. The von Hippel-Lindau gene was under-expressed but not significantly. CONCLUSIONS:A procedure for collecting and storing fresh renal tissue and subsequent gene expression profiling of RCC and normal renal tissue was established. A commercially available DNA macroarray coupled with the significance analysis of macroarrays allowed the identification of sets of differentially expressed cancer-related genes that were characteristic of RCC, compared with apparently normal renal tissue, and which distinguished among subgroups divided according to tumour grade and histological subtype. Quantitative PCR is important to validate the results of macroarray experiments.
journal_name
BJU Intjournal_title
BJU internationalauthors
Lovisolo JA,Casati B,Clerici L,Marafante E,Bono AV,Celato N,Salvadore Mdoi
10.1111/j.1464-410X.2006.06209.xsubject
Has Abstractpub_date
2006-07-01 00:00:00pages
205-16issue
1eissn
1464-4096issn
1464-410Xpii
BJU6209journal_volume
98pub_type
杂志文章abstract:OBJECTIVE:To highlight the existence of Schistosoma haematobium in certain ethnic minority groups in Britain and in English citizens who have recently visited Africa and the Middle East, so that general practitioners and paediatric nephrologists/urologists are aware of its occurrence and consider it among the different...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2000.00477.x
更新日期:2000-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the long-term outcome in children who had an artificial urinary sphincter (AUS) placed, after a minimum of 10 years of follow-up. PATIENTS AND METHODS:The medical records of patients who had an AUS placed at the Children's Hospital of Michigan were reviewed and a telephone questionnaire was then ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.1999.00077.x
更新日期:1999-06-01 00:00:00
abstract::The immediate objective of phosphodiesterase type 5 (PDE5) inhibitor treatment is to restore the ability of a man to achieve and/or maintain an erection adequate for sexual intercourse. As erectile dysfunction (ED) generally develops in the second half of life, the ultimate objective generally is not procreation, but ...
journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2008.08267.x
更新日期:2009-05-01 00:00:00
abstract:OBJECTIVES:To describe the step-by-step learning curve of the holmium laser enucleation of the prostate (HoLEP) surgical technique. SUBJECTS/PATIENTS AND METHODS:A prospective, multicentre observational study was conducted, involving surgeons experienced in transurethral resection of the prostate and open prostatectom...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/bju.13124
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVE:To determine whether men with premature ejaculation who fail to respond to 25 mg clomipramine as needed improve when taking 10-30 mg clomipramine daily. SUBJECTS AND METHODS:Four men with premature ejaculation whose ejaculation latencies increased minimally or not at all when taking 25 mg clomipramine as nee...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2001.00112.x
更新日期:2001-03-01 00:00:00
abstract:OBJECTIVE:To report a national questionnaire survey of all consultant urologists, providing a 'snapshot' of attitudes, current practice and referral patterns for radical prostatectomy (RP) in Britain and Ireland at the millennium. METHODS:During 1999/2000 a simple questionnaire about RP was sent to the 487 urologists ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2002.02906.x
更新日期:2002-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the difference between radiographic size on computed tomography (CT) and the pathological size of renal tumours, in contemporary patients. PATIENTS AND METHODS:We retrospectively reviewed the records of 521 patients undergoing surgical resection of a renal mass between 2000 and 2007, who had tumo...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2008.07941.x
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:To assess the cost, quality of life (QoL) and cost-effectiveness of a single-incision mini-sling (SIMS; Ajust(®) , C. R. Bard Inc., New Providence, NJ, USA) compared with a standard mid-urethral sling (SMUS; TVT-O™, Ethicon Inc., Somerville, NJ, USA) in the surgical management of female stress urinary incont...
journal_title:BJU international
pub_type: 杂志文章,随机对照试验
doi:10.1111/bju.12388
更新日期:2013-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2008.08240.x
更新日期:2009-05-01 00:00:00
abstract:OBJECTIVES:To explore the role of transient receptor potential vanilloid 1 (TRPV1) in the excitatory effects of chronic administration of nerve growth factor (NGF) on bladder-generated sensory input and reflex activity. To explore new therapeutic targets for bladder dysfunction. MATERIALS AND METHODS:Wild-type (WT) an...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2012.11187.x
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of dutasteride and tamsulosin as combined therapy compared with each monotherapy for improving patient-reported health outcomes in men with moderate-to-severe urinary symptoms and prostate enlargement, reporting the pre-planned 2-year analyses from the CombAT trial. PATIENTS AND MET...
journal_title:BJU international
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1464-410X.2009.08196.x
更新日期:2009-04-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.14923
更新日期:2020-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.14226
更新日期:2018-05-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2009.08880.x
更新日期:2010-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09713.x
更新日期:2011-06-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/bju.12291
更新日期:2013-11-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2001.00997.x
更新日期:2001-01-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2010.09273.x
更新日期:2010-10-01 00:00:00
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更新日期:2003-04-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2010-09-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2005.05714.x
更新日期:2005-10-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2001.02338.x
更新日期:2001-09-01 00:00:00
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pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2012.11775.x
更新日期:2013-09-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12559
更新日期:2014-05-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2012.11456.x
更新日期:2012-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06728.x
更新日期:2007-04-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2008.07697.x
更新日期:2008-08-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.15051
更新日期:2020-06-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12519
更新日期:2014-11-01 00:00:00
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pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2012.11483.x
更新日期:2013-04-01 00:00:00