Abstract:
:The Drosophila oocyte develops from a cluster of 16 interconnected cells that derive from a common progenitor. One of these cells, the oocyte, arrests in meiosis. The other cells endoreplicate their DNA and produce mRNAs and proteins that they traffic to the oocyte along a polarized microtubule cytoskeleton shared by the entire cyst. Therefore, Drosophila oogenesis is an attractive system for the study of cell cycle control and cell polarity. We carried out a clonal screen on the right arm of chromosome 3 for female sterile mutations using the FLP-FRT-ovo(D) system to identify new genes required for early oogenesis. We identified alleles of oo18 RNA binding protein (orb) and Darkener of apricot (Doa), which had previously been shown to exhibit oogenesis defects. We also identified several lethal alleles of the male sterile mutant, bobble (bob). In addition, we identified eight new lethal complementation groups that exhibit early oogenesis phenotypes. We analyzed mutant clones to determine the aspects of oogenesis disrupted by each complementation group. We assayed for the production and development of egg chambers, localization of ORB to and within the oocyte, and proper execution of the nurse cell cycle (endoreplication of DNA) and the oocyte cell cycle (karyosome formation). Here we discuss the identification, mapping, and phenotypic characterization of these new genes: omelet, soft boiled, hard boiled, poached, fried, over easy, sunny side up, and benedict.
journal_name
Geneticsjournal_title
Geneticsauthors
Morris JZ,Navarro C,Lehmann Rkeywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
1435-46issue
4eissn
0016-6731issn
1943-2631journal_volume
164pub_type
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