Abstract:
:Penicillium marneffei is an opportunistic fungal pathogen of humans and the only dimorphic species identified in its genus. At 25 degrees P. marneffei exhibits true filamentous growth, while at 37 degrees P. marneffei undergoes a dimorphic transition to produce uninucleate yeast cells that divide by fission. Members of the STE12 family of regulators are involved in controlling mating and yeast-hyphal transitions in a number of fungi. We have cloned a homolog of the S. cerevisiae STE12 gene from P. marneffei, stlA, which is highly conserved. The stlA gene, along with the A. nidulans steA and Cryptococcus neoformans STE12alpha genes, form a distinct subclass of STE12 homologs that have a C2H2 zinc-finger motif in addition to the homeobox domain that defines STE12 genes. To examine the function of stlA in P. marneffei, we isolated a number of mutants in the P. marneffei-type strain and, in combination with selectable markers, developed a highly efficient DNA-mediated transformation procedure and gene deletion strategy. Deletion of the stlA gene had no detectable effect on vegetative growth, asexual development, or dimorphic switching in P. marneffei. Despite the lack of a detectable function, the P. marneffei stlA gene complemented the sexual defect of an A. nidulans steA mutant. In addition, substitution rate estimates indicate that there is a significant bias against nonsynonymous substitutions. These data suggest that P. marneffei may have a previously unidentified cryptic sexual cycle.
journal_name
Geneticsjournal_title
Geneticsauthors
Borneman AR,Hynes MJ,Andrianopoulos Akeywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
1003-14issue
3eissn
0016-6731issn
1943-2631journal_volume
157pub_type
杂志文章相关文献
GENETICS文献大全abstract::Signaling by the second messenger inositol 1,4,5-trisphosphate is thought to affect several developmental and physiological processes. Mutants in the inositol 1,4,5-trisphosphate receptor (itpr) gene of Drosophila exhibit delays in molting while stronger alleles are also larval lethal. In a freshly generated set of EM...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.1.225
更新日期:2004-01-01 00:00:00
abstract::We found that heterozygous mutant alleles of E(Pc) and esc increased homologous recombination from an allelic template in somatic cells in a P-element-induced double-strand break repair assay. Flies heterozygous for mutant alleles of these genes showed increased genome stability and decreased levels of apoptosis in im...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.042473
更新日期:2006-04-01 00:00:00
abstract::To study the functions of DNA topoisomerase I and Hpr1 protein, a suppressor mutant of the temperature-sensitive growth of an hpr1 top1-5ts double mutant was isolated. The isolated triple mutant showed cold-sensitive growth. By complementation of this phenotype, the suppressor gene was cloned. DNA sequencing showed it...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::Translocation induced in the immature oocyte, in meiotic prophase, affects division I orientation and segregation, the usual result being that the two halves of translocations are directed to opposite poles. Since interchange is usually (if not exclusively) between chromatids, this is to be expected from the creation ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-09-01 00:00:00
abstract::We estimated DNA sequence variation in a 5.7-kb fragment of the furrowed (fw) gene region within and between four populations of Drosophila ananassae; fw is located in a chromosomal region of very low recombination. We analyzed gene flow between these four populations along a latitudinal transect on the Indian subcont...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Interactions between Drosophila C virus (DCV) and its natural host, Drosophila melanogaster, were investigated using 15 geographical population samples infected by intraabdominal inoculation. These strains derived from natural populations of D. melanogaster differed in susceptibility to the DCVc. One strain was "parti...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Hybrid matings between the sexual species Poeciliopsis monacha and Poeciliopsis lucida produced a series of diploid all-female lineages of P. monacha-lucida that inhabit the Río Fuerte of northwestern Mexico. Restriction site analyses of mitochondrial DNA (mtDNA) clearly revealed that P. monacha was the maternal ances...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::A scheme for rapidly mapping chromosome rearrangements relative to the physical map of Caenorhabditis elegans is described that is based on hybridization patterns of cloned DNA on meiotic nuclei, as visualized by fluorescent in situ hybridization. From the nearly complete physical map, DNA clones, in yeast artificial ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract::Genes with unstable transcripts often encode proteins that play important regulatory roles. ATL2 is a member of a multigene family coding highly related RING-H2 zinc-finger proteins that may function as E3 ubiquitin ligases. ATL2 mRNA accumulation occurs rapidly and transiently after incubation with elicitors of patho...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.028043
更新日期:2004-06-01 00:00:00
abstract::The RecQ helicase family is critical during DNA damage repair, and mutations in these proteins are associated with Bloom, Werner, or Rothmund-Thompson syndromes in humans, leading to cancer predisposition and/or premature aging. In the budding yeast Saccharomyces cerevisiae, mutations in the RecQ homolog, SGS1, phenoc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.157370
更新日期:2013-12-01 00:00:00
abstract::mod(mdg4), also known as E(var)3-93D, is involved in a variety of processes, such as gene silencing in position effect variegation (PEV), the control of gypsy insulator sequences, regulation of homeotic gene expression, and programmed cell death. We have isolated a large number of mod(mdg4) cDNAs, representing 21 diff...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Crossovers (COs) between homologous chromosomes are critical for meiotic chromosome segregation and form in the context of the synaptonemal complex (SC), a meiosis-specific structure that assembles between aligned homologs. During Caenorhabditis elegans meiosis, central region components of the SC (SYP proteins) are e...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302625
更新日期:2019-10-01 00:00:00
abstract::Levels of neutral variation are influenced by background selection and hitchhiking. The relative contribution of these evolutionary forces to the distribution of neutral variation is still the subject of ongoing debates. Using 133 microsatellites, we determined levels of variability on X chromosomes and autosomes in A...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Several hypotheses and concepts have tended to oversimplify the problem of mutagenesis and can be misleading when used for genetic risk estimation. These include: (1) the hypothesis that radiation-induced mutation frequency depends primarily on the DNA content per haploid genome, (2) the extension of this concept to c...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract::The vacuolar hydrolase protease B in Saccharomyces cerevisiae is synthesized as an inactive precursor (Prb1p). The precursor undergoes post-translational modifications while transiting the secretory pathway. In addition to N- and O-linked glycosylations, four proteolytic cleavages occur during the maturation of Prb1p....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::We have analyzed pregastrulation cleavage patterns in Caenorhabditis elegans embryos homozygous for various chromosomal deficiencies. By two different estimates these deficiencies represent between 37 and 49% of the genome, including the entire X chromosome and substantial portions of each of the five autosomes. Among...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Seventeen lines, each homozygous for a different X chromosome but all with a common autosomal genetic blackground, were constructed and assayed for abdominal bristle number to determine whether dosage compensation operates for sex-linked genes affecting this character. --The regression coefficient of male mean on fema...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Dna2 is a nuclease and helicase that functions redundantly with other proteins in Okazaki fragment processing, double-strand break resection, and checkpoint kinase activation. Dna2 is an essential enzyme, required for yeast and mammalian cell viability. Here, we report that numerous mutations affecting the DNA damage ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300809
更新日期:2018-05-01 00:00:00
abstract::We studied P element-induced recombination in germline mitotic cells by examining the structure of the recombinant chromosomes. We found that most recombinants retain a mobile P element at the site of the recombination, usually with either a deletion or a duplication immediately adjacent to the P end at which the cros...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::OrthoList, a compendium of Caenorhabditis elegans genes with human orthologs compiled in 2011 by a meta-analysis of four orthology-prediction methods, has been a popular tool for identifying conserved genes for research into biological and disease mechanisms. However, the efficacy of orthology prediction depends on th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301307
更新日期:2018-10-01 00:00:00
abstract::We have used the results of an experiment mapping quantitative trait loci (QTL) affecting milk yield and composition to estimate the total number of QTL affecting these traits. We did this by estimating the number of segregating QTL within a half-sib daughter design using logic similar to that used to estimate the "fa...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.077784
更新日期:2007-10-01 00:00:00
abstract::Actin is a major component of the cytoskeleton and one of the most abundant proteins found in eukaryotic cells. Comparative sequence analysis shows that this essential gene has been highly conserved throughout eukaryotic evolution making it useful for phylogenetic analysis. Complete cDNA clones for the actin-encoding ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::Mating type interconversion in Saccharomyces cerevisiae occurs by transposition of copies of the a or alpha mating type cassettes from inactive loci, HML and HMR, to an active locus, MAT. The lack of expression of the a and alpha genes at the silent loci results from repression by trans-acting regulators encoded by SI...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::Immature spermatids from Caenorhabditis elegans are stimulated by an external activation signal to reorganize their membranes and cytoskeleton to form crawling spermatozoa. This rapid maturation, termed spermiogenesis, occurs without any new gene expression. To better understand this signal transduction pathway, we is...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::The Caenorhabditis elegans gene gld-1 is essential for oocyte development; in gld-1 (null) hermaphrodites, a tumor forms where oogenesis would normally occur. We use genetic epistasis analysis to demonstrate that tumor formation is dependent on the sexual fate of the germline. When the germline sex determination pathw...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::The Genetics Society of America's (GSA) Edward Novitski Prize is awarded to researchers who have solved challenging problems in genetics through experiments that demonstrate exceptional creativity and ingenuity. Job Dekker of the University of Massachusetts Medical School has been selected for the 2018 award in recogn...
journal_title:Genetics
pub_type: 传,历史文章,杂志文章
doi:10.1534/genetics.118.301641
更新日期:2018-11-01 00:00:00
abstract::The behaviour of two "meiotic drive" systems, Segregation-Distorter (SD) and the sex chromosome sc(4)sc(8) has been examined in the same meiocyte. It has been found that the two systems interact in a specific way. When the distorting effects of SD and sc(4)sc(8) are against each other, there is no detectable interacti...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-09-01 00:00:00
abstract::We have cloned and disrupted in vivo, by repeat-induced point mutations, the nuclear gene coding for an iron sulfur subunit of complex I from Neurospora crassa, homologue of the mammalian TYKY protein. Analysis of the obtained mutant nuo21.3c revealed that complex I fails to assemble. The peripheral arm of the enzyme ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::A sib analysis of adult life-history characters was performed on about twelve hundred females from a laboratory Drosophila melanogaster population that had been sampled from nature and cultured so as to preserve its genetic variability. The following results were found. There was no detectable trend with age in additi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Yeast cells subjected to a reversible thermal arrest of meiosis yielded progressively fewer spores per ascus as the arrest was extended. Dissection of two-spored asci by a newly developed method that prevents selection of false asci revealed that the spores were not a random sample of the haploid meiotic products. Mos...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00