Reactivity-based one-pot total synthesis of fucose GM1 oligosaccharide: a sialylated antigenic epitope of small-cell lung cancer.

abstract：:Bacteriophage display of antibodies provides a method for the generation of immunological reagents against rare and uncharacterized antigens. To ascertain the usefulness of this approach for the characterization of inner-ear proteins, we produced a bacteriophage-displayed antibody-fragment library directed against pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cyr JL,Hudspeth AJ

更新日期：2000-02-29 00:00:00

abstract：:The degradation of the plant cell wall by glycoside hydrolases is central to environmentally sustainable industries. The major polysaccharides of the plant cell wall are cellulose and xylan, a highly decorated β-1,4-xylopyranose polymer. Glycoside hydrolases displaying multiple catalytic functions may simplify the enz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKee LS,Peña MJ,Rogowski A,Jackson A,Lewis RJ,York WS,Krogh KB,Viksø-Nielsen A,Skjøt M,Gilbert HJ,Marles-Wright J

更新日期：2012-04-24 00:00:00

abstract：:A screening procedure based on solvent extraction with minimal clean up followed by examination with negative chemical ionization mass spectrometry has been developed for organochlorine residues and related compounds with masses greater than 130 daltons. The procedure has been made quantitative for analysis of pentach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dougherty RC,Piotrowska K

更新日期：1976-06-01 00:00:00

abstract：:A posttranslational protein O-mannosylation process resembling that found in fungi and animals has been reported in the major human pathogen Mycobacterium tuberculosis (Mtb) and related actinobacteria. However, the role and incidence of this process, which is essential in eukaryotes, have never been explored in Mtb. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu CF,Tonini L,Malaga W,Beau M,Stella A,Bouyssié D,Jackson MC,Nigou J,Puzo G,Guilhot C,Burlet-Schiltz O,Rivière M

更新日期：2013-04-16 00:00:00

abstract：:Congenital osteopetrosis in the mutant rat "op" is accompanied by early atrophy of the thymus gland. The response of thymocytes to concanavalin A and phytohemagglutinin P was greatly diminished at age 28 days, even before pronounced thymic atrophy could be detected. The response of spleen cells to mitogens that stimul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milhaud G,Labat ML,Parant M,Damais C,Chedid L

更新日期：1977-01-01 00:00:00

abstract：:Inhibition of eicosanoid formation in larvae of the tobacco hornworm Manduca sexta, using specific inhibitors of phospholipase A2, cyclooxygenase, and lipoxygenase, severely weakened the ability of larvae to clear the bacterium Serratia marscescens from their hemolymph. The reduced capability to remove bacteria is ass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanley-Samuelson DW,Jensen E,Nickerson KW,Tiebel K,Ogg CL,Howard RW

更新日期：1991-02-01 00:00:00

abstract：:Gene regulation is a process with many steps allowing for stochastic biochemical reactions, which leads to expression noise-i.e., the cell-to-cell stochastic fluctuation in protein abundance. Such expression noise can give rise to drastically diverse phenotypes, even within isogenic cell populations. Although numerous...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li J,Min R,Vizeacoumar FJ,Jin K,Xin X,Zhang Z

更新日期：2010-06-08 00:00:00

abstract：:We previously have identified a subpopulation of contact-insensitive (CS-) cells which lacks density-dependent inhibition of cell division in primary and low-passage cultures of Syrian hamster embryonic (SHE) fibroblastic cells. Further, we have shown that the proportion of these CS- cells declines as a result of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano S,Ueo H,Bruce SA,Ts'o PO

更新日期：1985-08-01 00:00:00

abstract：:One of the strategies used by organisms to adapt to life under conditions of short energy supply is to use the by-product pyrophosphate to support cation gradients in membranes. Transport reactions are catalyzed by membrane-integral pyrophosphatases (PPases), which are classified into two homologous subfamilies: H(+)-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luoto HH,Baykov AA,Lahti R,Malinen AM

更新日期：2013-01-22 00:00:00

abstract：:Human genetic studies have given evidence of familial, disease-causing mutations in the analogous amino acid residue shared by three related RNA binding proteins causative of three neurological diseases. Alteration of aspartic acid residue 290 of hnRNPA2 to valine is believed to predispose patients to multisystem prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murray DT,Zhou X,Kato M,Xiang S,Tycko R,McKnight SL

更新日期：2018-10-16 00:00:00

abstract：:The polymerase chain reaction was used to amplify DNA surrounding the codon 12 region of the c-Ki-ras gene from C3H/10T1/2 cells and from a number of 3-methylcholanthrene (MCA)-transformed derivatives of these cells. Sequence analysis demonstrated that tumorigenic MCAC116/39 cells, known by DNA-mediated transfection t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen AC,Herschman HR

更新日期：1989-03-01 00:00:00

abstract：:The in vivo biological activities of IgG antibodies result from their bifunctional nature, in which antigen recognition by the Fab is coupled to the effector and immunomodulatory diversity found in the Fc domain. This diversity, resulting from both amino acid and glycan heterogeneity, is translated into cellular respo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith P,DiLillo DJ,Bournazos S,Li F,Ravetch JV

更新日期：2012-04-17 00:00:00

abstract：:Most of our knowledge about transmission at central synapses has been obtained by studying populations of synapses, but some important properties of synapses can be determined only by studying them individually. An important issue is whether a presynaptic action potential causes, at most, a single vesicle to be releas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conti R,Lisman J

更新日期：2003-04-15 00:00:00

abstract：:Amphiregulin (AR) and cripto are proteins that are structurally related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). AR is also functionally related to this family of growth regulatory molecules and is able to bind and activate the 170-kDa EGF receptor (EGFR). Human EGFR-3 (HER3)/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ciardiello F,Kim N,Saeki T,Dono R,Persico MG,Plowman GD,Garrigues J,Radke S,Todaro GJ,Salomon DS

更新日期：1991-09-01 00:00:00

abstract：:Blindsight is a phenomenon in which human patients with damage to striate cortex deny any visual sensation in the resultant visual field defect but can nonetheless detect and localize stimuli when persuaded to guess. Although monkeys with striate lesions have also been shown to exhibit some residual vision, it is not ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore T,Rodman HR,Repp AB,Gross CG

更新日期：1995-08-29 00:00:00

abstract：:We have previously extracted a protein from inflammatory mouse granuloma that fully protects normal mice against lethal Listeria monocytogenes infection. We now report that polyclonal antibodies directed against this protein react with a human urinary fraction that also provides full protection of normal mice against ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fontan E,Saklani-Jusforgues H,Fauve RM

更新日期：1992-05-15 00:00:00

abstract：:The assembly of the three neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key step that leads to exocytotic fusion of synaptic vesicles. In the fully assembled SNARE complex, these three proteins form a coiled-coil ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang B,Kiessling V,Tamm LK

更新日期：2013-11-26 00:00:00

abstract：:Rat cortical astrocytes in pure culture are functionally coupled to neighboring cells via connexin (Cx) 43 gap junctions under ordinary conditions. Small fluorescent molecules such as Lucifer yellow (LY) pass between cell interiors via gap junctions, but do not enter the cells when externally applied. Subjecting rat a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Contreras JE,Sánchez HA,Eugenin EA,Speidel D,Theis M,Willecke K,Bukauskas FF,Bennett MV,Sáez JC

更新日期：2002-01-08 00:00:00

abstract：:We have previously shown that the G protein of vesicular stomatitis virus (VSV-G) can be incorporated into the virions of retroviruses. Since expression of VSV-G is toxic to most mammalian cells, development of stable VSV-G packaging cell lines requires inducible VSV-G expression. We have modified the tetracycline-ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen ST,Iida A,Guo L,Friedmann T,Yee JK

更新日期：1996-09-17 00:00:00

abstract：:Mapping biological pathways across microbial genomes is a highly important technique in functional studies of biological systems. Existing methods mainly rely on sequence-based orthologous gene mapping, which often leads to suboptimal mapping results because sequence-similarity information alone does not contain suffi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao F,Su Z,Olman V,Dam P,Liu Z,Xu Y

更新日期：2006-01-03 00:00:00

abstract：:Plants can defend themselves from herbivorous insects by emitting volatile chemical signals that attract natural enemies of the herbivore. For example, maize seedlings attacked by beet armyworm larvae (Spodoptera exigua) produce a mixture of terpenoid and indole volatiles that serve to attract parasitic wasps. A key s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen B,Zheng Z,Dooner HK

更新日期：2000-12-19 00:00:00

abstract：:The Kell blood group is a major antigenic system in human erythrocytes. Kell antigens reside on a 93-kDa membrane glycoprotein that is surface-exposed and associated with the underlying cytoskeleton. We isolated tryptic peptides and, based on the amino acid sequence of one of the peptides and by using the PCR, prepare...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee S,Zambas ED,Marsh WL,Redman CM

更新日期：1991-07-15 00:00:00

abstract：:A type of chromosome-free cell called SimCells (simple cells) has been generated from Escherichia coli, Pseudomonas putida, and Ralstonia eutropha. The removal of the native chromosomes of these bacteria was achieved by double-stranded breaks made by heterologous I-CeuI endonuclease and the degradation activity of end...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan C,Davison PA,Habgood R,Zeng H,Decker CM,Gesell Salazar M,Lueangwattanapong K,Townley HE,Yang A,Thompson IP,Ye H,Cui Z,Schmidt F,Hunter CN,Huang WE

更新日期：2020-03-24 00:00:00

abstract：:Because previous studies showed that polyunsaturated fatty acids can reduce the contraction rate of spontaneously beating heart cells and have antiarrhythmic effects, we examined the effects of the fatty acids on the electrophysiology of the cardiac cycle in isolated neonatal rat cardiac myocytes. Exposure of cardiomy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang JX,Xiao YF,Leaf A

更新日期：1995-04-25 00:00:00

abstract：:Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this process, we have cloned the nuclear gene encoding the Mbb1 factor by genomic rescue of a mutant specifically deficient in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vaistij FE,Boudreau E,Lemaire SD,Goldschmidt-Clermont M,Rochaix JD

更新日期：2000-12-19 00:00:00

abstract：:Apicomplexan parasites replicate within a protective organelle, called the parasitophorous vacuole (PV). The Toxoplasma gondii PV is filled with a network of tubulated membranes, which are thought to facilitate trafficking of effectors and nutrients. Despite being critical to parasite virulence, there is scant mechani...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beraki T,Hu X,Broncel M,Young JC,O'Shaughnessy WJ,Borek D,Treeck M,Reese ML

更新日期：2019-03-26 00:00:00

abstract：:Metabolic, infectious, and tumor cell-intrinsic noxae can all evoke the endoplasmic reticulum (ER) stress response in tumor cells, which is critical for tumor cell growth and cancer progression. Evidence exists that the ER stress response can drive a proinflammatory program in tumor cells and macrophages but, to our k...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahadevan NR,Rodvold J,Sepulveda H,Rossi S,Drew AF,Zanetti M

更新日期：2011-04-19 00:00:00

abstract：:Symbiotic microbes impact the severity of a variety of diseases through regulation of T-cell development. However, little is known regarding the molecular mechanisms by which this is accomplished. Here we report that a secreted factor, Erdr1, is regulated by the microbiota to control T-cell apoptosis. Erdr1 expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soto R,Petersen C,Novis CL,Kubinak JL,Bell R,Stephens WZ,Lane TE,Fujinami RS,Bosque A,O'Connell RM,Round JL

更新日期：2017-05-23 00:00:00

abstract：:Global supply chains play a critical role in many of the most pressing environmental stresses and social struggles identified by the United Nations' Sustainable Development Goals (SDGs). Responding to calls from the global community, companies are adopting a variety of voluntary practices to improve the environmental ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thorlakson T,de Zegher JF,Lambin EF

更新日期：2018-02-27 00:00:00

abstract：:DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis, (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of replication points ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker DL,Glaser DA

更新日期：1975-06-01 00:00:00