Mouse model recapitulating human Fcγ receptor structural and functional diversity.

abstract：:Solar emission produces copious nitrosonium ions (NO(+)) in the D layer of the ionosphere, 60 to 90 km above the Earth's surface. NO(+) is believed to transfer its charge to water clusters in that region, leading to the formation of gaseous nitrous acid (HONO) and protonated water cluster. The dynamics of this reactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He R,Li L,Zhong J,Zhu C,Francisco JS,Zeng XC

更新日期：2016-04-26 00:00:00

abstract：:l-Serine biosynthesis, a crucial metabolic process in most domains of life, is initiated by d-3-phosphoglycerate (d-3-PG) dehydrogenation, a thermodynamically unfavorable reaction catalyzed by d-3-PG dehydrogenase (SerA). d-2-Hydroxyglutarate (d-2-HG) is traditionally viewed as an abnormal metabolite associated with c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang W,Zhang M,Gao C,Zhang Y,Ge Y,Guo S,Guo X,Zhou Z,Liu Q,Zhang Y,Ma C,Tao F,Xu P

更新日期：2017-09-05 00:00:00

abstract：:Although cells express hundreds of metalloenzymes, the mechanisms by which apoenzymes receive their metal cofactors are largely unknown. Poly(rC)-binding proteins PCBP1 and PCBP2 are multifunctional adaptor proteins that bind iron and deliver it to ferritin for storage or to prolyl and asparagyl hydroxylases to metall...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frey AG,Nandal A,Park JH,Smith PM,Yabe T,Ryu MS,Ghosh MC,Lee J,Rouault TA,Park MH,Philpott CC

更新日期：2014-06-03 00:00:00

abstract：:We have identified a protein present only in erythroid cells that binds to two adjacent sites within an enhancer region of the chicken beta-globin locus. Mutation of the sites, so that binding by the factor can no longer be detected in vitro, leads to a loss of enhancing ability, assayed by transient expression in pri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Evans T,Reitman M,Felsenfeld G

更新日期：1988-08-01 00:00:00

abstract：:Many higher level avian clades are restricted to Earth's lower latitudes, leading to historical biogeographic reconstructions favoring a Gondwanan origin of crown birds and numerous deep subclades. However, several such "tropical-restricted" clades (TRCs) are represented by stem-lineage fossils well outside the ranges...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saupe EE,Farnsworth A,Lunt DJ,Sagoo N,Pham KV,Field DJ

更新日期：2019-06-25 00:00:00

abstract：:Plant mortality and birth rates are critical components of plant life history affecting the stability of plant populations and the ecosystems they form. Although allometric theory predicts that both plant birth and mortality rates should be size-dependent, this prediction has not yet been tested across plants ranging ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marbà N,Duarte CM,Agustí S

更新日期：2007-10-02 00:00:00

abstract：:Transgenic sorghum plants have been obtained after microprojectile bombardment of immature zygotic embryos of a drought-resistant sorghum cultivar, P898012. DNA delivery parameters were optimized based on transient expression of R and C1 maize anthocyanin regulatory elements in scutellar cells. The protocol for obtain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casas AM,Kononowicz AK,Zehr UB,Tomes DT,Axtell JD,Butler LG,Bressan RA,Hasegawa PM

更新日期：1993-12-01 00:00:00

abstract：:Protection of poikilothermic animals from seasonal cold is widely regarded as being causally linked to changes in the unsaturation of membrane phospholipids, yet in animals this proposition remains formally untested. We have now achieved this by the genetic manipulation of lipid biosynthesis of Caenorhabditis elegans ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murray P,Hayward SA,Govan GG,Gracey AY,Cossins AR

更新日期：2007-03-27 00:00:00

abstract：:Mutator activity associated with the common male recombination (MR) chromosomes in Drosophila melanogaster appears to be suppressed in natural populations. Crosses between geographically separated populations, however, lead to the release of mutator activity as measured by a significant increase in visible mutations. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson JN Jr,Woodruff RC

更新日期：1980-02-01 00:00:00

abstract：:The Ritz upper bound to eigenvalues of Hermitian operators is essential for many applications in science. It is a staple of quantum chemistry and physics computations. The lower bound devised by Temple in 1928 [G. Temple, Proc. R. Soc. A Math. Phys. Eng. Sci. 119, 276-293 (1928)] is not, since it converges too slowly....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martinazzo R,Pollak E

更新日期：2020-07-14 00:00:00

abstract：:Although sexual selection theory has proved successful in explaining a wide array of male ornaments, the function of ornaments occurring in females is largely unknown. Traditionally, female ornaments have been considered nonfunctional, being merely a genetically correlated response to selection for male ornamentation....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amundsen T,Forsgren E

更新日期：2001-11-06 00:00:00

abstract：:In typical force spectroscopy experiments, a small biomolecule is attached to a soft polymer linker that is pulled with a relatively large bead or cantilever. At constant force, the total extension stochastically changes between two (or more) values, indicating that the biomolecule undergoes transitions between two (o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cossio P,Hummer G,Szabo A

更新日期：2015-11-17 00:00:00

abstract：:The kinetics of charge transfer depend crucially on the dielectric reorganization of the medium. In enzymatic reactions that involve charge transfer, atomic dielectric response of the active site and of its surroundings determines the efficiency of the protein as a catalyst. We report direct spectroscopic measurements...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mertz EL,Krishtalik LI

更新日期：2000-02-29 00:00:00

abstract：:The E1A gene of adenovirus type 5 encodes two major proteins of 289 and 243 amino acid residues, which are identical except that the larger protein has an internal stretch of 46 amino acids required for efficient trans-activation of early viral promoters. This domain contains a consensus zinc finger motif (Cys-Xaa2-Cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Culp JS,Webster LC,Friedman DJ,Smith CL,Huang WJ,Wu FY,Rosenberg M,Ricciardi RP

更新日期：1988-09-01 00:00:00

abstract：:Dyskeratosis congenita is a rare inherited disorder characterized by abnormal skin manifestations. Morbidity and mortality from this disease is usually due to bone marrow failure, but idiopathic pulmonary fibrosis and an increased cancer predisposition also occur. Families with autosomal dominant dyskeratosis congenit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armanios M,Chen JL,Chang YP,Brodsky RA,Hawkins A,Griffin CA,Eshleman JR,Cohen AR,Chakravarti A,Hamosh A,Greider CW

更新日期：2005-11-01 00:00:00

abstract：:Elucidation of mechanisms that regulate hematopoietic stem cell self-renewal and differentiation would be facilitated by the identification of defined culture conditions that allow these cells to be amplified. We now demonstrate a significant net increase (3-fold, P < 0.001) in vitro of cells that are individually abl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller CL,Eaves CJ

更新日期：1997-12-09 00:00:00

abstract：:To scrutinize how a protein folds at atomic resolution, we performed 200 molecular dynamics simulations (each of 50 ns) of the miniprotein Trp-cage on the computational grid. Within the trajectories, 58 folding and 31 unfolding events were identified and subjected to extensive comparison and classification. Based on a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ota M,Ikeguchi M,Kidera A

更新日期：2004-12-21 00:00:00

abstract：:WOPR-domain proteins are found throughout the fungal kingdom where they function as master regulators of cell morphology and pathogenesis. Genetic and biochemical experiments previously demonstrated that these proteins bind to specific DNA sequences and thereby regulate transcription. However, their primary sequence s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohse MB,Rosenberg OS,Cox JS,Stroud RM,Finer-Moore JS,Johnson AD

更新日期：2014-07-22 00:00:00

abstract：:Leptin acts as a potent inhibitory factor against obesity by regulating energy expenditure, food intake, and adiposity. The obese diabetic db/db mouse, which has defects in leptin receptor, displays enhanced neural responses and elevated behavioral preference to sweet stimuli. Here, we show the effects of leptin on th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawai K,Sugimoto K,Nakashima K,Miura H,Ninomiya Y

更新日期：2000-09-26 00:00:00

abstract：:The tumor promoter phorbol 12-myristate 13-acetate (PMA) induces characteristic reversible changes of cell shape in certain fibroblastic lines: motile lamellas are transformed into noncontractile narrow processes; simultaneously, the actin microfilament network of lamellas is locally disorganized. This reaction to PMA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bershadsky AD,Ivanova OY,Lyass LA,Pletyushkina OY,Vasiliev JM,Gelfand IM

更新日期：1990-03-01 00:00:00

abstract：:A 1,942-base-pair DNA segment encoding the structural gene for diphtheria toxin was sequenced, and the primary structure of the toxin was deduced. Restriction enzyme fragments corresponding to nontoxic or hypotoxic peptides of the toxin were isolated from corynebacteriophage beta and cloned into Escherichia coli on pl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greenfield L,Bjorn MJ,Horn G,Fong D,Buck GA,Collier RJ,Kaplan DA

更新日期：1983-11-01 00:00:00

abstract：:In the presence of the S polypeptide, maize chloroplast DNA-dependent RNA polymerase preferentially transcribes sequences within the 2200-nucleotide-pair-long maize chloroplast chromosome fragment Eco [unk] from a supercoiled chimeric plasmid cloned in Escherichia coli [Jolly, S. O. & Bogorad, L. (1980) Proc. Natl. Ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwarz Z,Jolly SO,Steinmetz AA,Bogorad L

更新日期：1981-06-01 00:00:00

abstract：:Two functional domains in the primary structure of basic fibroblast growth factor (FGF) have been identified on the basis of their ability to interact with the FGF receptor, bind radiolabeled heparin, and modulate the cellular response to FGF. Peptides derived from these two functional domains can act as partial agoni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baird A,Schubert D,Ling N,Guillemin R

更新日期：1988-04-01 00:00:00

abstract：:GTP hydrolysis by elongation factor Tu (EF-Tu), a translational GTPase that delivers aminoacyl-tRNAs to the ribosome, plays a crucial role in decoding and translational fidelity. The basic reaction mechanism and the way the ribosome contributes to catalysis are a matter of debate. Here we use mutational analysis in co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maracci C,Peske F,Dannies E,Pohl C,Rodnina MV

更新日期：2014-10-07 00:00:00

abstract：:Chinese hamster-human somatic cell hybrids were analyzed for the expression of human galactose-1-phosphate uridyltransferase (GALT; UDPglucose:alpha-D-galactose-1-phosphate uridyltransferase, EC 2.7.7.12) by electrophoresis and for the presence of human chromosomes cytogenetically with the aid of Q-banding. Three of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohandas T,Sparkes RS,Sparkes MC,Shulkin JD

更新日期：1977-12-01 00:00:00

abstract：:Rett syndrome (RTT) is a severe neurodevelopmental disorder that affects about 1 in 10,000 female live births. The underlying cause of RTT is mutations in the X-linked gene, methyl-CpG-binding protein 2 (MECP2); however, the molecular mechanism by which these mutations mediate the RTT neuropathology remains enigmatic....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Osenberg S,Karten A,Sun J,Li J,Charkowick S,Felice CA,Kritzer M,Nguyen MVC,Yu P,Ballas N

更新日期：2018-06-05 00:00:00

abstract：:Four overlapping cosmid clones were constructed that contain the complete genome of the attenuated Oka strain of VZV. Transfection of human melanoma cells with the four cosmids resulted in production of infectious VZV. A double-stranded oligonucleotide, encoding a stop codon in all three open reading frames, was inser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen JI,Seidel KE

更新日期：1993-08-01 00:00:00

abstract：:Chromosome 14 was transferred into tumorigenic nasopharyngeal carcinoma and esophageal carcinoma cell lines by a microcell-mediated chromosome transfer approach. Functional complementation of defects present in the cancer cells suppressed tumor formation. A candidate tumor-suppressor gene, cysteine-rich intestinal pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung AK,Ko JM,Lung HL,Chan KW,Stanbridge EJ,Zabarovsky E,Tokino T,Kashima L,Suzuki T,Kwong DL,Chua D,Tsao SW,Lung ML

更新日期：2011-05-17 00:00:00

abstract：:The temperature coefficient of equilibrium isotope fractionation in the heavy elements is shown to be larger at high temperatures than that expected from the well-studied vibrational isotope effects. The difference in the isotopic behavior of the heavy elements as compared with the light elements is due to the large n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bigeleisen J

更新日期：1996-09-03 00:00:00

abstract：:We report the heavy chain variable region sequences from the cDNAs of five previously described monoclonal cell lines producing human antibodies specific for the human immunodeficiency virus type 1 and detail the molecular characteristics, germ-line origins, and extent of somatic mutation among these antibodies. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andris JS,Johnson S,Zolla-Pazner S,Capra JD

更新日期：1991-09-01 00:00:00