Effect of growth conditions on DNA-membrane attachment in Escherichia coli.

abstract：:Purified fractions of human apotransferrin, monoferric transferrins with iron on the acid-labile binding site and on the acid-stable binding site, and diferric transferrin have been prepared. The iron loading and unloading behavior of these preparations has been examined by isoelectric focusing. Iron release from the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebers H,Josephson B,Huebers E,Csiba E,Finch C

更新日期：1981-04-01 00:00:00

abstract：:The autoimmune dermatologic disease pemphigus vulgaris (PV) is associated with the HLA serotypes DR4 and DRw6. Susceptibility to PV could be conferred either by sequences shared between the DR4 and DRw6 haplotypes or by different sequences in these haplotypes. We have examined the distribution of DR and DQ beta-chain ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scharf SJ,Friedmann A,Brautbar C,Szafer F,Steinman L,Horn G,Gyllensten U,Erlich HA

更新日期：1988-05-01 00:00:00

abstract：:To achieve accurate aminoacylation of tRNAs with their cognate amino acids, errors in aminoacylation are corrected by the "editing" mechanism in several aminoacyl-tRNA synthetases. Phenylalanyl-tRNA synthetase (PheRS) hydrolyzes, or edits, misformed tyrosyl-tRNA with its editing domain in the beta subunit. We report t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasaki HM,Sekine S,Sengoku T,Fukunaga R,Hattori M,Utsunomiya Y,Kuroishi C,Kuramitsu S,Shirouzu M,Yokoyama S

更新日期：2006-10-03 00:00:00

abstract：:The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koken MH,Reynolds P,Jaspers-Dekker I,Prakash L,Prakash S,Bootsma D,Hoeijmakers JH

更新日期：1991-10-15 00:00:00

abstract：:Recombinant adenoviral mediated delivery of suicide and cytokine genes has been investigated as a treatment for hepatic metastases of colon carcinoma in mice. Liver tumors were established by intrahepatic implantation of a poorly immunogenic colon carcinoma cell line (MCA-26), which is syngeneic in BALB/c mice. Intrat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caruso M,Pham-Nguyen K,Kwong YL,Xu B,Kosai KI,Finegold M,Woo SL,Chen SH

更新日期：1996-10-15 00:00:00

abstract：:The amounts of mRNAs encoding alpha 1, alpha 6, beta 2, beta 3, gamma 2, and delta subunits of gamma-aminobutyrate type A (GABAA) receptors and the gold immunolabeling density of their translation products were monitored during the growth of neonatal rat granule cells in primary culture. We investigated possible corre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng TM,Zhu WJ,Puia G,Vicini S,Grayson DR,Costa E,Caruncho HJ

更新日期：1994-11-08 00:00:00

abstract：:Circular RNA replicons have been reported in plants and, in one case, in animal cells. We describe such an element in yeast. In certain yeast strains, a 20S RNA species appears on transfer of cells to acetate medium. This phenotype shows cytoplasmic (non-Mendelian) inheritance and the 20S RNA is associated with 23-kDa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsumoto Y,Fishel R,Wickner RB

更新日期：1990-10-01 00:00:00

abstract：:The biconcave disk shape and deformability of mammalian RBCs rely on the membrane skeleton, a viscoelastic network of short, membrane-associated actin filaments (F-actin) cross-linked by long, flexible spectrin tetramers. Nonmuscle myosin II (NMII) motors exert force on diverse F-actin networks to control cell shapes,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith AS,Nowak RB,Zhou S,Giannetto M,Gokhin DS,Papoin J,Ghiran IC,Blanc L,Wan J,Fowler VM

更新日期：2018-05-08 00:00:00

abstract：:An intermolecular intercalation of base pairs was found at the CA step in the I222 crystal structure of the RNA.DNA hybrid, r(CAAAGAAAAG).d(CTTTTCTTTG), which contains two-thirds of the polypurine tract sequence of HIV-1 with a substitution of cytosine for the initial adenine. This sequence crystallized in both P21212...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han GW,Kopka ML,Langs D,Sawaya MR,Dickerson RE

更新日期：2003-08-05 00:00:00

abstract：:Protected areas are crucial for biodiversity conservation because they provide safe havens for species threatened by land-use change and resulting habitat loss. However, protected areas are only effective when they stop habitat loss within their boundaries, and are connected via corridors to other wild areas. The effe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radeloff VC,Stewart SI,Hawbaker TJ,Gimmi U,Pidgeon AM,Flather CH,Hammer RB,Helmers DP

更新日期：2010-01-12 00:00:00

abstract：:The phosphorylation of diacylglycerol (DG), a reaction catalyzed by DG kinase, may be critical in the termination of effector-induced signals mediated by protein kinase C. Synapsin I is a principal target of intracellular protein kinases and is thought to be involved in the release of neurotransmitter from axon termin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kahn DW,Besterman JM

更新日期：1991-07-15 00:00:00

abstract：:Over the last two decades, the number of gene/protein sequences gleaned from sequencing projects of individual genomes and environmental DNA has grown exponentially. Only a tiny fraction of these predicted proteins has been experimentally characterized, and the function of most proteins remains hypothetical or only pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Helbert W,Poulet L,Drouillard S,Mathieu S,Loiodice M,Couturier M,Lombard V,Terrapon N,Turchetto J,Vincentelli R,Henrissat B

更新日期：2019-03-26 00:00:00

abstract：:Normal myeloid precursors and MGI(+)D(+) myeloid leukemic cells can be induced to differentiate to mature cells by the normal protein inducer MGI. The sequence of differentiation is the induction of C3 and Fc rosettes, C3 and Fc immune phagocytosis (IP), synthesis and secretion of lysozyme, and formation of mature mac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lotem J,Sachs L

更新日期：1977-12-01 00:00:00

abstract：:Previous work identified the Rap1 GTPase-activating protein Sipa1 as a germ-line-encoded metastasis modifier. The bromodomain protein Brd4 physically interacts with and modulates the enzymatic activity of Sipa1. In vitro analysis of a highly metastatic mouse mammary tumor cell line ectopically expressing Brd4 demonstr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crawford NP,Alsarraj J,Lukes L,Walker RC,Officewala JS,Yang HH,Lee MP,Ozato K,Hunter KW

更新日期：2008-04-29 00:00:00

abstract：:We partially purified a preparation from Escherichia coli that proteolytically degrades the enzyme glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]. The degradation is at least a two-step process. First, the glutamine synthetase undergoes an oxidative modification. This modification leads to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine RL,Oliver CN,Fulks RM,Stadtman ER

更新日期：1981-04-01 00:00:00

abstract：:Salt and water secretion from intestinal epithelia requires enhancement of anion permeability across the apical membrane of Cl- secreting cells lining the crypt, the secretory gland of the intestine. Paneth cells located at the base of the small intestinal crypt release enteric defensins (cryptdins) apically into the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lencer WI,Cheung G,Strohmeier GR,Currie MG,Ouellette AJ,Selsted ME,Madara JL

更新日期：1997-08-05 00:00:00

abstract：:Interleukin (IL)-1beta is a pluripotent proinflammatory cytokine that signals through the type-I IL-1 receptor (IL-1RI), a member of the Toll-like receptor family. In hypothalamic neurons, binding of IL-1beta to IL-1RI mediates transcription-dependent changes that depend on the recruitment of the cytosolic adaptor pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis CN,Mann E,Behrens MM,Gaidarova S,Rebek M,Rebek J Jr,Bartfai T

更新日期：2006-02-21 00:00:00

abstract：:Soft corals of the family Xeniidae exhibit a unique, rhythmic pulsation of their tentacles (Movie S1), first noted by Lamarck nearly 200 y ago. However, the adaptive benefit of this perpetual, energetically costly motion is poorly understood. Using in situ underwater particle image velocimetry, we found that the pulsa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kremien M,Shavit U,Mass T,Genin A

更新日期：2013-05-28 00:00:00

abstract：:The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berlyn MB,Last RL,Fink GR

更新日期：1989-06-01 00:00:00

abstract：:The highly homologous Rnf2 (Ring1b) and Ring1 (Ring1a) proteins were identified as in vivo interactors of the Polycomb Group (PcG) protein Bmi1. Functional ablation of Rnf2 results in gastrulation arrest, in contrast to relatively mild phenotypes in most other PcG gene null mutants belonging to the same functional gro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voncken JW,Roelen BA,Roefs M,de Vries S,Verhoeven E,Marino S,Deschamps J,van Lohuizen M

更新日期：2003-03-04 00:00:00

abstract：:Group II intron RNAs self-splice in vitro but only at high salt and/or Mg2+ concentrations and have been thought to require proteins to stabilize their active structure for efficient splicing in vivo. Here, we show that a DEAD-box protein, CYT-19, can by itself promote the splicing and reverse splicing of the yeast aI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohr S,Matsuura M,Perlman PS,Lambowitz AM

更新日期：2006-03-07 00:00:00

abstract：:In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environment to an anaerobic s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sciandra JJ,Subjeck JR,Hughes CS

更新日期：1984-08-01 00:00:00

abstract：:Cells in the brain act as components of extended networks. Therefore, to understand neurobiological processes in a physiological context, it is essential to study them in vivo. Super-resolution microscopy has spatial resolution beyond the diffraction limit, thus promising to provide structural and functional insights ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turcotte R,Liang Y,Tanimoto M,Zhang Q,Li Z,Koyama M,Betzig E,Ji N

更新日期：2019-05-07 00:00:00

abstract：:In the presence of the nucleotide analog adenosine 5'-[gamma-thio]triphosphate (ATP[gamma S]), effects of Ca2+ on stiffness and equatorial x-ray diffraction patterns of single skinned fibers of the rabbit psoas muscle were studied. It is shown that cross-bridges in the presence of ATP[gamma S] have properties of the w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kraft T,Yu LC,Kuhn HJ,Brenner B

更新日期：1992-12-01 00:00:00

abstract：:Little is known about how genetic variation and epigenetic marks interact to shape differences in behavior. The foraging (for) gene regulates behavioral differences between the rover and sitter Drosophila melanogaster strains, but the molecular mechanisms through which it does so have remained elusive. We show that th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anreiter I,Kramer JM,Sokolowski MB

更新日期：2017-11-21 00:00:00

abstract：:Evidence for hydrogen bonding between 5'-ribonucleotides in water has been obtained from a 220-MHz proton magnetic resonance study of nitrogenous protons. The amino groups of GMP, AMP, and CMP exhibit proton resonance lines which are somewhat broadened by proton exchange with the solvent at 0 degrees ; their downfield...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raszka M,Kaplan NO

更新日期：1972-08-01 00:00:00

abstract：:The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the DNA lesions 1-methyladenine and 3-methylcytosine, which are generated in single-stranded stretches of DNA. AlkB is an alpha-ketoglutarate- and Fe(II)-dependent dioxygenase that oxidizes the relevant methyl groups...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duncan T,Trewick SC,Koivisto P,Bates PA,Lindahl T,Sedgwick B

更新日期：2002-12-24 00:00:00

abstract：:Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotwein P

更新日期：1986-01-01 00:00:00

abstract：:Purified RNA polymerase II (pol II) from the yeast Saccharomyces cerevisiae pauses without releasing at many locations during in vitro transcription. Pausing can be induced by intrinsic DNA sequence as well as by specific DNA bound proteins such as the RNA pol I termination factor, Reb1p, or lac repressor. Addition of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lang WH,Platt T,Reeder RH

更新日期：1998-04-28 00:00:00

abstract：:Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schuster H,Mikolajczyk M,Rohrschneider J,Geschke B

更新日期：1975-10-01 00:00:00