Abstract:
:Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC.
journal_name
Naturejournal_title
Natureauthors
Rawat UB,Zavialov AV,Sengupta J,Valle M,Grassucci RA,Linde J,Vestergaard B,Ehrenberg M,Frank Jdoi
10.1038/nature01224keywords:
subject
Has Abstractpub_date
2003-01-02 00:00:00pages
87-90issue
6918eissn
0028-0836issn
1476-4687pii
nature01224journal_volume
421pub_type
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