Abstract:
:Two cytosolic fractions (A and B) from Xenopus oocytes are sufficient to support protein import into the nuclei of digitonin-permeabilized cells. Fraction A recognizes the nuclear localization sequence (NLS) and binds the import substrate to the nuclear envelope, whereas fraction B mediates the subsequent passage of the bound substrate into the nucleus. Here we report that two interacting components are required for full fraction-B activity, purify one of these components to homogeneity, and show that it is the highly abundant GTP-binding protein Ran (Ras-related nuclear protein)/TC4.
journal_name
Naturejournal_title
Natureauthors
Moore MS,Blobel Gdoi
10.1038/365661a0subject
Has Abstractpub_date
1993-10-14 00:00:00pages
661-3issue
6447eissn
0028-0836issn
1476-4687journal_volume
365pub_type
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