Abstract：
OBJECTIVE:To investigate whether the decrease in expression of interleukin-2 (IL-2) after trauma is associated with changes in DNA binding activity of nuclear factor of activated T cells (NFAT) and activator protein-1 (AP-1). METHODS:Mice with closed impact injury with fracture in both hind limbs were adopted as the trauma model. Spleen lymphocytes were isolated from traumatized mice and stimulated with Con-A. Culture supernatants were assayed for IL-2 activity, and total RNA was extracted from spleen lymphocytes and assayed for IL-2 mRNA. DNA binding activity of NFAT and AP-1 were measured by electrophoretic mobility shift assay (EMSA). The expression of c-Fos, c-Jun and JunB proteins was determined by the Western blot analysis. RESULTS:DNA binding activity of NFAT and AP-1 gradually decreased to a minimum of 41% and 49%, respectively, of the control on the 4th day after injury, which was closely followed by the decline in IL-2 activity and IL-2 mRNA. A decrease in the expression of c-Fos on the 1st and 4th day after trauma had no significant effect on c-Jun expression; the increase in expression of JunB was only on the 1st day after injury. CONCLUSION:Decreased IL-2 expression is, at least in part, due to a decline in the activation of NFAT and AP-1 in traumatized mice. The decline in DNA binding activity of NFAT and AP-1 is partly due to a trauma-induced block in the expression of c-Fos.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Luo Y,Liang H,Hu C,Xu X,Wang Zkeywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
1348-51issue
9eissn
0366-6999issn
2542-5641journal_volume
115pub_type
杂志文章abstract：:Aluminium citrate (Al-cit) was shown to be biologically effective in preventing silicosis. A total of 917 cases selected from both engineering corps of railway and coalmines with stage 0 or 0+ by radiographs were studied. Cases were randomly divided into Al-cit treatment group and control group with continuing dust ex...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1990-02-01 00:00:00
abstract：BACKGROUND:The decreased degradation of extra-cellular matrix proteins plays an important role in the onset of diabetic nephropathy. Matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1), which are members of the matrix metalloproteinase family, are associated with this process. Angiot...
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pub_type： 杂志文章
doi：
更新日期：2007-11-05 00:00:00
abstract：:Cellular and humoral immune functions in Chinese hemophiliacs with and without human immunodeficiency virus (HIV) infection were compared with those of healthy controls. There was a significant increase in peripheral T8 cells and a significant decrease of peripheral T4 cells as well as the T4/T8 ratio in 3 hemophiliac...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：OBJECTIVE:To analyze the effect and mechanism of hyperbaric oxygen (HBO) treatment for severe brain injury (SBI). METHODS:Fifty-five patients were divided into a treatment group of 35 patients and a control group of 20 patients. We observed the alterations of clinical, brain electric earth map (BEAM), endothelin (ET) ...
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doi：
更新日期：2001-04-01 00:00:00
abstract：BACKGROUND:C-arm-based flat-panel detector cone-beam computed tomography (CBCT) venography has never been used in the management of iliac vein compression syndrome (IVCS). This study aimed to determine the technical feasibility and safety of CBCT venography in the diagnosis of IVCS compared with conventional venography...
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doi：10.1097/CM9.0000000000001046
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2007-12-05 00:00:00
abstract：BACKGROUND:Osteoarthritis (OA) is the most common form of human polyarthritis. Many genetic factors have been implicated in OA. It was reported that a polymorphism in the gene of interleukin-6 (IL-6) was associated with OA of knee. The aim of this study was to determine whether functional IL-6 promoter -174G/C (rs18007...
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pub_type： 杂志文章,meta分析
doi：
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journal_title：Chinese medical journal
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doi：
更新日期：2006-06-05 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
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更新日期：2010-01-05 00:00:00
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doi：
更新日期：2007-04-05 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：1997-11-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2001-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1097/CM9.0000000000000752
更新日期：2020-05-20 00:00:00
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doi：
更新日期：2003-12-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：OBJECTIVE:To test the immunosuppressive effect of cyclosporine (Cs) in a polymer placed in the anterior chamber of corneal allograft recipients. METHODS:Wistar inbred rats with vascularized corneas were recipients of corneal allografts from Sprague-Dawley donor rats. Rats underwent penetrating keratoplasty and were di...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:This review summarizes recent evidences regarding the potential influences of epidural labor analgesia (ELA) on the outcomes of neonates and children. Terms and relevant words including "ELA," "ELA and neonatal outcomes," "ELA and children's development," and "ELA and children's neurocognitive development" were used t...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000900
更新日期：2020-10-05 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：1998-05-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：2005-10-05 00:00:00
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pub_type： 临床试验,杂志文章
doi：
更新日期：2009-09-20 00:00:00
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doi：
更新日期：2012-06-01 00:00:00
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更新日期：2013-11-01 00:00:00
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doi：10.1097/CM9.0000000000001256
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更新日期：2016-01-05 00:00:00
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pub_type： 杂志文章
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更新日期：2004-06-01 00:00:00
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