Abstract:
:We examined the effects of suppressing multidrug resistance-associated protein (MRP) and multidrug resistance 1 (MDR1) gene expression in HCT-8DDP human colon cancer cell lines, which showed both cisplatin and multidrug resistance. Hammerhead ribozymes, designed to cleave MRP mRNA (anti-MRP Rz) and MDR1 mRNA (anti-MDR1 Rz), were transfected into the HCT-8DDP cells. Drug sensitivity was estimated by MTT assay in vitro. The HCT-8DDP/anti-MRP Rz cells were more sensitive to doxorubicin (DOX) and etoposide (VP-16) by 2.5- and 4.1-fold, respectively, compared with HCT-8DDP cells. The HCT-8DDP/anti-MDR Rz cells were more sensitive to DOX and VP-16 by 2.3- and 3.8-fold, respectively. The anti-MRP Rz and anti-MDR1 Rz significantly down-regulated resistance to DOX and VP-16, while anti-MRP Rz and anti-MDR1 Rz did not affect resistance to cisplatin, methotrexate and 5-fluorouracil. The hammerhead ribozyme-mediated specific suppression of MRP or MDR1 was sufficient to reverse multidrug resistance in the human colon cancer cell line.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Nagata J,Kijima H,Hatanaka H,Asai S,Miyachi H,Abe Y,Yamazaki H,Nakamura M,Watanabe N,Mine T,Kondo T,Scanlon KJ,Ueyama Ykeywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
1021-6issue
5eissn
1019-6439issn
1791-2423journal_volume
21pub_type
杂志文章abstract::Specific chromosomal abnormalities, such as del(7q), t(12;14), 12 trisomy, or the rearrangement of 6p, are seen in approximately 30% of uterine leiomyomas despite their benign status. We investigated the association between the shrinkage of uterine leiomyomas treated with a GnRH agonist and the interstitial deletion o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.6.1259
更新日期:2001-06-01 00:00:00
abstract::Recently, the inflammatory cytokine IL-6 has been reported as a potent inducer of epithelial-mesenchymal transition (EMT) in breast cancer cells with an epithelial phenotype. Furthermore, EMT induces stem cell features in normal and transformed mammary cells. We explored whether IL-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1275
更新日期:2012-04-01 00:00:00
abstract::Functional defects in DNA mismatch repair genes have been shown to be associated mainly with hereditary human malignancies. We examined genomic DNA from 88 sporadic transitional cell carcinomas (TCCs) of the urinary tract for mutations in hMSH6 gene by polymerase chain reaction and direct sequencing analysis. Mutation...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::The homeobox transcription factor orthodenticle homeobox 2 (OTX2) plays a critical role in very early neurogenesis, but can become oncogenic when aberrantly expressed later in life. We previously discovered its novel oncogenic role in the malignant childhood brain tumor medulloblastoma and hypothesize an oncogenic rol...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3179
更新日期:2015-11-01 00:00:00
abstract::The tyrosine kinase inhibitor imatinib has been reported to inhibit CD8+ T lymphocytes. Little is known about its effects on CD4+CD25+ regulatory T cells (T(reg) cells) which might regulate the graft-vs.-leukemia (GVL) reaction after allogeneic stem cell transplantation (allo-SCT) and donor lymphocyte infusion (DLI). ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::The effect of immune cells on the growth and development of human prostatic cancer cells was investigated. Cell proliferation of androgen-independent human prostatic cancer cells JCA-1 was reduced 30-40% with the supplementation of a growth modulating activity present in lymphocyte conditioned medium. The slower growi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.979
更新日期:1994-10-01 00:00:00
abstract::Hypoxia, a common phenomenon during the development of malignant solid tumors including breast cancer, serves to propagate a cascade of molecular pathways triggered by hypoxia-inducible factor-1α (HIF-1α). Carbonic anhydrase IX (CA IX), one of the target genes of HIF-1α, has been reported to be involved in progression...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3253
更新日期:2016-01-01 00:00:00
abstract::We have previously shown that the genotoxin-induced apoptosis in mouse embryo fibroblasts was enhanced by the extracellular matrix protein fibronectin (FN). In the present study, we tested the hypothesis that FN regulates the DNA damage response (DDR) signaling pathways in HCT116 (p53-wt) and HT29 (p53-mut) human colo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1034
更新日期:2011-08-01 00:00:00
abstract::Accumulating evidence indicates that microRNAs (miRNAs) have a critical role in cell proliferation and metastasis in hepatocellular carcinoma (HCC). However, the effect of miR‑300 on the development and progression of HCC remains unclear. In the present study, it was observed that miRNA (miR)‑300 expression was signif...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4715
更新日期:2019-04-01 00:00:00
abstract::Chemotherapy is an important treatment option for gastric cancer (GC); however, chemotherapy usually fails due to drug resistance, particularly multidrug resistance (MDR). In our previous studies, microRNA (miR)‑874 was demonstrated to serve an important role in tumour growth, apoptosis and angiogenesis. In the presen...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4593
更新日期:2018-12-01 00:00:00
abstract::Cytokine production in squamous cell carcinoma (SCC) of the head and neck may directly influence the clinical behavior of this aggressive neoplasm since cytokines act as autocrine/paracrine growth factors, as well as immunomodulators. In the present study, the cytokine profiles of 16 SCCs of the head and neck and matc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Interleukin (IL)-4 and IL-10 have a wide variety of activities in the immune system. We re-evaluated the action of IL-4 and IL-10 on human blood monocytes, myeloid dendritic cell (DC1) precursors, using a serum-free culture system. Both IL-4 and IL-10 inhibited the survival of CD14+ monocytes supported by granulocyte-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract::Hsp90α is a molecular chaperone protein involved in the structural maturation of oncogenic signaling proteins. Hsp90 was recently identified as an anticancer target; various studies are ongoing to find ways for managing cancer through Hsp90α. However, this approach is limited by reported side-effects. Hypoxia is a hal...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1450
更新日期:2012-07-01 00:00:00
abstract::5-Fluorouracil (5-FU), a fluoropyrimidine analogue, is one of the most commonly used anticancer drugs for the treatment of gastrointestinal malignancies. Some studies reported that the cytotoxicity of fluoropyrimidines is mediated, in large part, by inhibition of the thymidylate synthase (TS), an essential DNA synthet...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Quinazolinone derivatives are known to possess anticancer activities on cell metastasis and cell death in different human cancer cell lines. Here, we studied the anti-metastasis activity and the underlying mechanisms of the novel quinazoline derivative MJ-56 (6-pyrrolidinyl-2-(3-bromostyryl)quinazolin-4-one). MJ-56 in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1941
更新日期:2013-07-01 00:00:00
abstract::Tetramethypyrazine (TMP), one of the active compounds extracted from the traditional Chinese medicinal herb (Chuanxiong), has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms have not been fully elucidated. In the present study, the anticancer ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3670
更新日期:2016-10-01 00:00:00
abstract::In this study we examined the levels of IL-1 alpha, IL-1 beta, IL-2, IL-6, TNF-alpha and sIL-2R in the sera and culture supernatants of PHA-stimulated lymphocytes from a series of 90 cancer patients. The expression of the IL-2R p55 chain (alpha subunit) on PHA-stimulated lymphocytes was also evaluated together with th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1211
更新日期:1994-12-01 00:00:00
abstract::Overexpression of inducible nitric oxide synthase (iNOS) and the resultant overproduction of NO has been implicated in neuronal inflammatory diseases. Leptomycin B (LMB), a metabolite of Streptomyces, has been identified as a specific inhibitor of CRM1 nuclear export receptor. In this study, we evaluated the effect of...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-12-01 00:00:00
abstract::In the multistep progressive pathogenesis of human breast cancer, comedo ductal carcinoma in situ (DCIS) represents a preinvasive precursor lesion for therapy resistant invasive cancer. Human tissue derived cell culture models exhibiting molecular similarities to clinical DCIS facilitate an important preclinical mecha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::Hypoxia regulates the expression of genes that promote tumor growth, angiogenesis and invasion. We previously studied hypoxic tumor cells in vitro and from hepatic metastases of colorectal cancer and determined several potential prognostic factors for hepatocellular carcinoma (HCC). In this study, we evaluated the pro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2519
更新日期:2014-09-01 00:00:00
abstract::The human prostate gland is a well known source of H1 and H2 relaxin but information is lacking on the expression and potential role of the INSL3 peptide hormone within the prostate gland. In the present study we have investigated the expression of human INSL3 in patients with benign prostate hyperplasia (BPH), prosta...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::The human immunodeficiency virus (HIV) protein negative factor (Nef) is important for AIDS pathogenesis. An anti-Nef single-domain antibody (sdAb19) derived from camelids has been previously generated and shown to effectively block the physiological functions of Nef in vitro and in vivo in nef-transgenic mice. However...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3132
更新日期:2015-10-01 00:00:00
abstract::Cordycepin (3'-deoxyadenosine), a major bioactive compound of Cordyceps militaris, has many pharmacological actions, such as anti-inflammatory and anticancer activities. In this study, the relationship between inhibition of cell motility and anti-invasive activity by cordycepin in L...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1332
更新日期:2012-05-01 00:00:00
abstract::Loss of chromosome sequences at 13q14 (Rbl) and 17p13 (p53) associated with squamous cell carcinoma of head and neck (SCCHN) was evaluated in 12 recurrent tumors and 51 primary tumors from 63 patients. The incidence of LOH at 17p13 was 19 of 50 (38%) tumors, and at 13q14 was 21 of 57 (37%). LOH affecting Rbl and/or p5...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.6.1235
更新日期:1997-06-01 00:00:00
abstract::We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.3.433
更新日期:2000-09-01 00:00:00
abstract::Hypericin is used as a powerful naturally occurring photosensitizer in photodynamic therapy (PDT). Activated by visible light, it kills tumour cells and tissues via generation of reactive oxygen species (ROS). Depending on the protocol, apoptotic cell death can be achieved very effectively by hypericin-PDT. To analyze...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000407
更新日期:2009-10-01 00:00:00
abstract::Over the past few decades, there has been a tremendous increase in cancer biology data and treatment. Cancer research has opened exciting new areas of cellular and molecular biology. Month by month, new genes which regulate the carcinogenesis process are being discovered. The result is an incredible knowledge of cance...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.15.6.1213
更新日期:1999-12-01 00:00:00
abstract::Quantitative nuclear morphometry (QNM) provides a reliable, reproducible method for measuring the degree of nuclear pleomorphism, a qualitative parameter widely incorporated into histopathological grading schemes. Nuclear shape has successfully been shown to predict primary prostate adenocarcinoma metastatic potential...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.3.673
更新日期:1995-09-01 00:00:00
abstract::MUC16 (CA125) is a transmembrane mucin that contributes to the progression of epithelial ovarian cancer (EOC). Expression of MUC16 is not detectable in the epithelial surface of normal ovaries. MUC16 expression is, however, common in serous EOC as well as in metastatic and recurrent tumors. Despite these observations,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2707
更新日期:2015-01-01 00:00:00
abstract::Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3643
更新日期:2016-10-01 00:00:00