Abstract:
BACKGROUND:Increased expression of proinflammatory cytokines, including tumour necrosis factor alpha, interleukin 6, and interferon gamma, as well as activation of proinflammatory signalling molecules such as nuclear factor kappa B, is characteristic of inflammatory bowel disease (IBD). AIMS:To investigate expression and activation of signal transducer and activator of transcription (STAT) 1 in patients with IBD. PATIENTS:Patients with active IBD (n=42), disease specificity controls (n=8), and normal controls (n=12) were investigated. METHODS:Expression and activation of STAT1 were assessed by western blotting and electrophoretic mobility shift assays in extracts of endoscopic colonic biopsies. Cellular localisation was determined by immunohistochemistry. RESULTS:Western blots and immunohistochemical staining revealed an increase in STAT1 expression and activation in mucosal samples from ulcerative colitis and to a lesser extend in Crohn's disease patients. High levels of suppressor of cytokine signalling (SOCS)-3 expression, an inhibitor of STAT activation, were observed in Crohn's disease patients and normal controls in western blot experiments whereas no differences were observed for SOCS-1 expression. Phosphorylated (p) STAT1 was mainly detected in monocytic cells and neutrophils in the inflamed mucosa. Induction of remission by systemic glucocorticoids led to a decrease in levels of pSTAT1. In vitro studies indicated a direct effect of steroid treatment on STAT1 activation. CONCLUSIONS:Expression and activation of STAT1 are predominantly heightened in ulcerative colitis and may therefore play an important role in the pathophysiology of colonic inflammation.
journal_name
Gutjournal_title
Gutauthors
Schreiber S,Rosenstiel P,Hampe J,Nikolaus S,Groessner B,Schottelius A,Kühbacher T,Hämling J,Fölsch UR,Seegert Ddoi
10.1136/gut.51.3.379keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
379-85issue
3eissn
0017-5749issn
1468-3288journal_volume
51pub_type
杂志文章相关文献
GUT文献大全abstract:BACKGROUND AND AIMS:Helicobacter pylori infection and gastric atrophy are both risk factors for gastric cancer. We aimed to elucidate the natural history of gastric cancer development according to H pylori infection and gastric atrophy status. SUBJECTS AND METHODS:A total of 9293 participants in a mass health appraisa...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2004.055400
更新日期:2005-06-01 00:00:00
abstract::To study the mechanism for the adaptive mucosal hyperplasia which occurs independent of luminal nutrition and pancreatico-biliary secretions in isolated Thiry-Vella segments of intestine from lactating rats, and to examine the effects of prolactin on small bowel mucosal structure in the rat, we used two models of expe...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.22.7.558
更新日期:1981-07-01 00:00:00
abstract::Psychological treatments are increasingly being used to help patients with the irritable bowel syndrome (IBS), but the efficacy of such treatments is still debated. This review indicates that there are three ways in which they might have been effective in published studies to date; relating bowel symptoms to stress, s...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.30.11.1601
更新日期:1989-11-01 00:00:00
abstract::This paper reviews the experience gained over the past few years in two techniques of liver support, charcoal haemoperfusion and polyacrylonitrile membrane haemodialysis. Problems with charcoal haemoperfusion have centred around platelet aggregation and hypertension, and new carbon preparations with a variety of diffe...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.19.6.578
更新日期:1978-06-01 00:00:00
abstract:BACKGROUND:Genetic haemochromatosis is a common hereditary iron loading disorder in humans. The disease is associated with loss of function mutations in the HFE gene. This is thought to change iron stores via increased iron absorption. AIMS:In this study we investigated how adaptation of mucosal reductase activity is ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.4.510
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVE:Gluten-free diet (GFD) is the only management for coeliac disease (CD). Available methods to assess GFD compliance are insufficiently sensitive to detect occasional dietary transgressions that may cause gut mucosal damage. We aimed to develop a method to determine gluten intake and monitor GFD compliance in p...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2015-310148
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND:Patients with chronic hepatitis B (CHB) infection are at an increased risk of developing hepatocellular carcinoma (HCC). Risk scores have been developed in Asian populations to predict HCC risk over time. AIM:To assess the performance of HCC risk prediction models in a heterogeneous population of patients w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2014-309099
更新日期:2016-08-01 00:00:00
abstract::The extent to which defects in protein synthesis occurred in an experimental indomethacin induced rat model of nonsteroidal enteropathy has been examined. Male rats (nine) were fed indomethacin (8 mg/kg/day) for three days mixed with a powdered form of chow. The control group of rats (nine) were fed the same diet for ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.2.242
更新日期:1992-02-01 00:00:00
abstract::The motor activity of the transverse, descending, and sigmoid colon was recorded for 24 hours in 14 healthy volunteers with a colonoscope positioned catheter. During the study the patients ate two 1000 kcal mixed meals and one continental breakfast. Colonic motor activity was low before meals and minimal during sleep;...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.28.1.17
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:Although severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was detected in faeces of patients with COVID-19, the activity and infectivity of the virus in the GI tract during disease course is largely unknown. We investigated temporal transcriptional activity of SARS-CoV-2 and its association wi...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2020-322294
更新日期:2021-02-01 00:00:00
abstract::The colonic biopsy specimens of 22 patients with colitis and positive stool cultures for Campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate Campylobacter colitis from chronic inflammatory bowel disease. In addition we tried to identify Campylobacter incl...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.9.945
更新日期:1985-09-01 00:00:00
abstract::A previous cohort study suggested that there might be an association between use of cimetidine and motor neurone disease. The Oxford Record Linkage Study was used to explore this hypothesis. In the analysis the presence of a peptic ulcer in patients after 1976 was taken as a proxy for cimetidine (and ranitidine) use. ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.34.12.1660
更新日期:1993-12-01 00:00:00
abstract:OBJECTIVE:Population-based studies demonstrating the clinical impact of interferon-free direct-acting antiviral (DAA) therapies are lacking. We examined the impact of the introduction of DAAs on HCV-related decompensated cirrhosis (DC) through analysis of population-based data from Scotland. DESIGN:Through analysis of...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-320007
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND AND AIMS:Crohn's disease (CD) and ulcerative colitis (UC) are chronic multifactorial inflammatory bowel diseases (IBDs) with unknown aetiology, but a deregulated mucosal immune response to gut-derived bacterial antigens is thought to be involved. Toll-like receptor ligands, especially lipopolysaccharide (LPS...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2007.128868
更新日期:2009-03-01 00:00:00
abstract::Fifty two patients with abnormal acid gastro-oesophageal reflux were studied by simultaneous oesophageal pH monitoring and continuous aspiration for 16 hours. Aspirates (from discrete two hour periods) were analysed for volume, pH, bile acids (conjugated and unconjugated), trypsin, and pepsin. The results were compare...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.32.10.1093
更新日期:1991-10-01 00:00:00
abstract:OBJECTIVES:Chronic gastro-oesophageal reflux might lead to the development of Barrett's oesophagus (BO) or even oesophageal adenocarcinoma. There has been no definitive systematic review and meta-analysis of data to estimate global prevalence of BO or oesophageal adenocarcinoma in individuals with gastro-oesophageal re...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2020-321365
更新日期:2020-07-30 00:00:00
abstract::A man with long-standing pernicious anaemia developed multiple gastric carcinoid tumours with a background of diffuse enterochromaffin-like cell hyperplasia. There is evidence that enterochromaffin-like cells synthesis and store histamine and that their proliferation is stimulated by high serum gastrin levels. Gastric...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.22.3.237
更新日期:1981-03-01 00:00:00
abstract::Gastric secretion from 24 subjects was collected by continuous nasogastric aspiration during the plateau of secretion evoked by intravenous histamine diphosphate, 0.01 mg/kg/hr. Simultaneously, phenol red was instilled at a constant rate into the proximal part of the stomach. Concentrations of phenol red in 15-minute ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.10.10.787
更新日期:1969-10-01 00:00:00
abstract::In duodenal ulceration the Alcian blue staining acid mucosubstances in goblet cells are reduced and the goblet cells themselves disappear. At the same time periodic acid-Schiff (PAS) staining neutral mucosubstances appear in the cells of the surface epithelium. These changes have been measured quantitatively with the ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.24.10.909
更新日期:1983-10-01 00:00:00
abstract::The metabolism of pure radioiodine labelled Clq has been observed in five patients with ulcerative colitis, five patients with Crohn's disease, and in five control subjects. Both the fractional catabolic rate and the synthesis rate of Clq were increased in the five patients with Crohn's disease and in four of the five...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.20.11.1012
更新日期:1979-11-01 00:00:00
abstract:BACKGROUND:Multiorgan failure is a severe life threatening state where present therapeutic approaches are suboptimal. Epidermal growth factor (EGF) is a potent stimulant of repair in in vitro and in vivo models. We therefore examined its potential beneficial effect in reducing mortality and injury induced by the noxiou...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.48.1.34
更新日期:2001-01-01 00:00:00
abstract::The spectrum of clinical disease associated with specific defects in jejunal brush border membrane sodium/proton exchange is poorly defined and only two patients have been described so far. Jejunal brush border membrane transport studies were performed in a boy who presented with lethal familial protracted diarrhoea i...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.10.1156
更新日期:1990-10-01 00:00:00
abstract:OBJECTIVE:Current strategies to guide selection of neoadjuvant therapy in oesophageal adenocarcinoma (OAC) are inadequate. We assessed the ability of a DNA damage immune response (DDIR) assay to predict response following neoadjuvant chemotherapy in OAC. DESIGN:Transcriptional profiling of 273 formalin-fixed paraffin-...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2018-317624
更新日期:2019-11-01 00:00:00
abstract::Six of 25 renal transplant recipients had a duodenal ulcer at endoscopy. Histamine concentration and the activity of histamine methyltransferase, the degrading enzyme of histamine, were measured in gastric mucosal biopsies obtained at endoscopy and compared with patients with duodenal ulcer but not undergoing transpla...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.1.65
更新日期:1989-01-01 00:00:00
abstract::Forty five patients with refractory oesophagitis, defined as persisting erosive changes or ulceration despite a minimum of three months' treatment with cimetidine 3.2 g daily or ranitidine 0.9 g daily, were treated in an open trial with omeprazole 40 mg daily for up to eight weeks. Endoscopically defined healing was o...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.7.745
更新日期:1990-07-01 00:00:00
abstract::Faecal bile acid excretion was examined in 13 patients with adenomatous polyps of the large bowel and compared with a series of matched healthy subjects. Bile acids were analysed in detail with respect to the composition of individual bile acids and their mode of conjugation. The total excretion of bile acids by the p...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.25.8.824
更新日期:1984-08-01 00:00:00
abstract:OBJECTIVE:As pathogen sensors, Toll-like receptors (TLR) play a role in the first defence line during HCV infection. However, the impact of the DNA sensor TLR9 on the natural course of HCV infection is unknown. To address this, TLR9 promoter polymorphisms (single nucleotide polymorphisms (SNPs)) rs187084 and rs5743836 ...
journal_title:Gut
pub_type: 杂志文章,多中心研究
doi:10.1136/gutjnl-2015-310239
更新日期:2017-10-01 00:00:00
abstract::The effect of sodium taurocholate instillation on the stimulated stomachs of patients with duodenal ulceration has been investigated. Instillation into the stomach of sodium taurocholate significantly reduced pentagastrin stimulated volume and outputs of both acid and sodium, with no change in the calculated volume of...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.24.1.28
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:Non-alcoholic fatty liver disease (NAFLD) is a frequent complication of morbid obesity, but its severity varies greatly and thus there is a strong need to better define its natural history in these patients. DESIGN:Liver biopsies were systematically performed in 798 consecutive patients with severe obesity u...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gutjnl-2016-312238
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:Studies on intestinal T cell clones from the mucosa of patients with coeliac disease have led to the identification of immunogenic gliadin epitopes. One is HLA-DQ8 restricted, its recognition by T cells being increased by introduction of negatively charged residues operated by tissue transglutaminase. AIM:T...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.1.57
更新日期:2003-01-01 00:00:00